In Vitro Mucolytic Activity of Cardamom Fruit (Amomum compactum) Decoction on Duck Egg Albumens

Desy Aryanti Pardilla Vitri, Siti Rahmatul Aini, Legis Ocktaviana Saputri

Abstract


Cardamom (Amomum compactum) is empirically used as a cough medicine by using its seeds and fruits. Cardamom seeds have been shown to have mucolytic activity at a concentration of 0.8%, while cardamom fruit has not been studied. This study aimed to determine the mucolytic activity of cardamom fruit decoction at variation concentrations. Mucolytic activity tests were carried out in vitro on duck egg albumens at 3%, 6%, and 12% variation concentrations of cardamom fruit decoction. N-acetylcysteine 0.1% was positive control, while phosphate buffer and Tween 80 were negative controls. The mucolytic activity was determined based on the viscosity value measured by flow time using an Ostwald viscometer and density measurement using a pycnometer. Data from each group were analyzed using SPSS with parametric One-Way ANOVA and Post Hoc tests. The results showed that cardamom fruit decoction at concentrations of 3%, 6%, and 12% had values that were not significantly different from N-acetylcysteine (p>0.05) and there was no significant difference (p>0.05) between each concentration group. Accordingly, the research concludes that cardamom fruit decoction at concentrations of 3%, 6%, and 12% has mucolytic activity comparable to N-acetylcysteine.

Keywords


Cardamom Fruit; Decoction; Duck Egg Albumens; In Vitro; Mucolytic

Full Text:

PDF

References


Abrami, M., Biasin, A., Tescione, F., Tierno, D., Dapas, B., Carbone, A., Grassi, G., Conese, M., Di Gioia, S., Larobina, D., & Grassi, M. (2024). Mucus Structure, Viscoelastic Properties, and Composition in Chronic Respiratory Diseases. International Journal of Molecular Sciences, 25(3). https://doi.org/10.3390/ijms25031933

Adil, M., Amim, S., & Mohtashin, M. (2018). N-acetylcysteine in Dermatology. Indian Journal of Dermatology, Venereology and Leprology, 84(1), 6–15. https://doi.org/10.4103/ijdvl.IJDVL

Anggraini, I. A. K. D., Darmayanti, L. P. T., & Sugitha, I. M. (2020). Pengaruh Lama Perebusan pada Pembuatan Minuman Herbal Daun Sawo (Manilkara zapota) Terhadap karakteristik dan Daya Hambat Pertumbuhan Escherichia coli. Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 9(3), 272. https://doi.org/10.24843/itepa.2020.v09.i03.p03

Chaiyasit, W., Brannan, R., Chareonsuk, D., & Chanasattru, W. (2019). Comparison of Physicochemical and Functional Properties of Chicken and Duck Egg Albumens. Brazilian Journal of Poultry Science, 21(1), 1–9.

Dahlan, S. M. (2010). Statistik untuk Kedokteran dan Kesehatan. Jakarta: Salemba Medika.

Depkes. (1989). Materia Medika Indonesia (Jilid V). Jakarta: Departemen Kesehatan Republik Indonesia.

Depkes RI. (1980). Materia Medika Edisi VI. Jakarta: Departemen Kesehatan Republik Indonesia.

Desiyana, L. S., Vonna, A., Hafsyari, R., Illian, D. N., & Koresponden, P. (2021). Uji Aktivitas Mukolitik Daun Kersen (Muntingia calabura L.) secara In Vitro. Indonesia. Jurnal Bioleuser, 5(3), 18–21. https://doi.org/https://doi.org/10.24815/j.%20bioleuser.v5i1.22977

Endarini, L. H. (2016). Farmakognosi dan Fitokimia. Jakarta: Kementrian Kesehatan Republik Indonesia.

Ernawati, Suharjon, & Fika, W. (2022). Budidaya Kapulaga (Amomum cardamomum). Kementerian Pertanian.

Fadilah, S., Mu’nisa, A., & Rachmawaty. (2018). Analisis Fitokimia Air Rebusan Daun Kayu Jawa (Lannea coromandelica). Bionature, 19(1), 73–77.

Ferdinan, A., Izzamaulana, Y., & Justicia, A. K. (2023). Uji Aktivitas Mukolitik Ekstrak Etanol Akar Bajakah (Spatholobus littoralis Hassk.) Secara in Vitro In Vitro Test of The Mucolytic Activity of The Ethanol Extract of Bajakah (Spatholobus littoralis Hassk .) Root. Journal Pharmasci, 8(2), 173–178.

Fitriyanti, R., Emmawati, E., & Yuliantini, A. (2022). Analisis Antosianin dari Buah dengan Berbagai Macam Pelarut Menggunakan Metode Spektrofotometeri UV-VIS. Jurnal Health Sains, 3(7), 812–818.

Fretes, F. de, Messakh, S. T., & Saogo, I. D. M. (2020). Manajemen Keluarga Terhadap Penanganan ISPA Berulang Pada Balita di Puskesmas Mangunsari Salatiga. Jurnal Sains Dan Kesehatan, 2(4), 275–281. https://doi.org/10.25026/jsk.v2i4.144

Hariana, H. A. (2013). 262 Tumbuhan Obat dan Khasiatnya. Cibubur: Penebar Swadya.

Kurniawati, D., Fitrianingsih, S. P., & Suwendar. (2020). Studi Literatur Aktivitas Mukolitik Beberapa Tanaman Genus Hibiscus dan Genus Piper terhadap Mukus Usus Sapi. Prosiding Farmasi, 6(2), 97–100.

Ladeska, V., Dwitiyanti, & Dilar, E. A. (2020). Aktivitas Mukolitik Fraksi Etanol Herba Meniran (Phyllanthus niruri L) Pada Mukosa Usus Sapi Secara In Vitro. Uhamka Repository .

Liu, H., Wang, W., & Gao, X. (2020). Comparison of The Efficacy of Ambroxol Hydrochloride and N?acetylcysteine in The Treatment of Children with Cronchopneumonia and Their Influence on Prognosis. Experimental and Therapeutic Medicine, 20(6), 1–1. https://doi.org/10.3892/etm.2020.9260

Mahmoudi, G. A., Astaraki, P., Mohtashami, A. Z., & Ahadi, M. (2015). N-acetylcysteine Overdose After Acetaminophen poisoning. International Medical Case Reports Journal, 8, 65–69. https://doi.org/10.2147/IMCRJ.S74563

Martin, A., Swarbrick, J., & Cammarata, A. (2016). Farmasi Fisik: Dasar-Dasar Kimia Fisik Dalam Ilmu Farmasetik Edisi Ketiga. UI-Press.

Mulyono. (2009). Membuat Reagen Kimia di Laboratorium. Jakarta: Bumi Kasara.

Nerdy, N., & Manurung, K. (2018). Mucolytic Activity of Rosella (Hibiscus sabdariffa L.) Calyces Edxtract on Cow Intestinal Mucus. Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, 12(2), 43–46. https://doi.org/10.21157/j.ked.hewan.v12i2.9621

Nofriyaldi, A., Adlina, S., & Rusmayanti, W. W. (2022). Formula dan Uji Aktivitas Antibakteri Sediaan Masker Gel Peel-Off Ekstrak Buah Kapulaga (Amomum compactum) terhadap Bakteri Propinium acnes. Seminar Nasional Penelirian Dan Pengabdian 2022, 39–50. https://ejurnal2.poltekkestasikmalaya.ac.id/index.php/prosidingpenelitian/article/view/96

Nofriyaldi, A., Nur Endah, S. R., & Mutiara, A. P. (2023). Efektivitas Daya Antibakteri Ekstrak Etanol Buah Kapulaga (Amomum compactum Sol. Ex Maton) Terhadap Pertumbuhan Propionibacterium acnes. Media Informasi, 19(1), 67–74. https://doi.org/10.37160/bmi.v19i1.123

Pakadang, S. R., Sinala, S., Salasa, A. M., Ratnah, S., Rosmala Dewi, S. T., & Hilaria, M. (2020). Potential of Miana Leaf Extract as Expectorant (Profile Place of Growing, Antioxidant, Sputum Contaminants, Antibacterial, MIC, MKC Expectorant). Majalah Obat Tradisional, 25(2), 94. https://doi.org/10.22146/mot.52500

Raaman, N. (2006). Phytochemical Techniques. India: New India Publishing Agency.

Raghu, G., Berk, M., Campochiaro, P. A., Jaeschke, H., Marenzi, G., Richeldi, L., Wen, F.-Q., Nicoletti, F., & Calverley, P. M. A. (2020). The Multifaceted Therapeutic Role of N-Acetylcysteine (NAC) in Disorders Characterized by Oxidative Stress. Current Neuropharmacology, 19(8), 1202–1224. https://doi.org/10.2174/1570159x19666201230144109

Rambe, R., Rani, Z., & Thomas, N. A. (2021). Uji Efektivitas Mukolitik Ekstrak Umbi Bawang Dayak (Eleutherine bulbosa (Mill) Urb). Journal Syifa Sciences and Clinical Research, 3(2), 71–77. https://doi.org/10.37311/jsscr.v3i2.7041

Riskesdas. (2018). Laporan Riskesdas 2018 Nasional. Jakarta: Kementerian Kesehatan Republik Indonesia.

Rowe, R. C., Sheskey, P. J., & Quinn, M. E. (2009). Handbook of Pharmaceutical Excipient. UK: Pharmaceutical Press.

Rumagit, H. M., Runtuwene, M. R., & Sudewi, S. (2015). Uji Fitokimia dan Uji Aktivitas Antioksidan dari Ekstrak Etanol Spons Lamellodysidea herbacea. Pharmacon, 4(3), 2302–2493.

Saminan. (2016). Efek Obstruksi Pada Saluran Pernapasan Terhadap Daya Kembang Paru. Jurnal Kedokteran Syiah Kuala, 16(1), 36–39. http://jurnal.unsyiah.ac.id/JKS/article/download/5010/4441

Sari, K., Sutoyo, S., & Hidajati, N. (2019). Uji Aktivitas Mukolitik Ekstrak Metanol Tumbuhan Paku Perak (Pityrogramma calomelanos ). Prosiding Seminar Nasional Kimia, 172–178.

Sulistanti, E., Pratama, I. S., Hidayati, A. R., & Wirasisya, D. G. (2022). Uji Aktivitas Mukolotik Rebusan Herba Putri Malu (Mimosa pudica L.) secara In Vitro. Jurnal Medika Udayana, 11(7), 56–60. https://doi.org/eum

Susiami, S., & Mubin, M. F. (2022). Peningkatan Bersihan Jalan Nafas Pada Anak Balita Penderita ISPA Dengan Terapi Uap Air Dan Minyak Kayu Putih Di Poliklinik AKPOL Semarang. Ners Muda, 3(1). https://doi.org/10.26714/nm.v3i1.7089

Sutoyo, S., Tukiran, & Sari, N. I. K. (2020). In Vitro Mucolytic Activity Assay of The Acetone Extract of The Silver Fern (Pityrogramma calomelanos). Atlantis Press, 390, 137–140. https://doi.org/10.2991/icracos-19.2020.29

Syaputra, R. A., Aini, S. R., & Juliantoni, Y. (2021). Aktivitas Mukolitik Sirup Ekstrak Etanolik Biji Kapulaga (Amomum compactum Sol. Ex Maton) pada Mukus Usus Sapi Secara In Vitro. Unram Medical Journal, 10(1), 384–390. https://doi.org/10.29303/jku.v10i1.504

Tenório, M. C. D. S., Graciliano, N. G., Moura, F. A., de Oliveira, A. C. M., & Goulart, M. O. F. (2021). N-acetylcysteine (NAC): Impacts on Human Health. Antioxidants, 10(6). https://doi.org/10.3390/antiox10060967

Tiwow, V. A. (2015). Analisis Aliran Fluida Netonian Pada Pipa Tidak Horizontal. JSPF Jilid, 11(1), 104–108.

Wahyuni, S., Vifta, R. L., & Erwiyani, A. R. (2018). Kajian Aktivitas Antibakteri Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Terhadap Pertumbuhan Streptococcus mutans. Jurnal Inovasi Teknik Kimia, 3(1), 25–30. https://doi.org/10.31942/inteka.v3i1.2122

Wahyuningtyas, A., Suyatno, & Hidajati, N. (2016). Uji Aktivitas Mukolitik Senyawa Flavonoid Hasil Isolasi dari Ekstrak Diklorometana Batang Tumbuhan Paku Chingia sakayensis. Prosiding Seminar Nasional Kimia Dan Pembelajarannya, 13–20.

Wati, L. K. (2017). Formulasi Sirup Ekstrak Daun Sendok (Plantago mayor L.) Sebagai Ekspektoran dengan Parameter Uji Mukolitik. Jurnal Ilmiah As-Syifaa, 9(1), 43–50. https://doi.org/10.33096/jifa.v9i1.240

WHO. (2020). Severe Acute Respiratory Infections Treatment Centre. Word Health Organization.

Windriyati, Y. N., Murrukmihadi, M., & Junita, N. R. (2019). Aktivitas Mukolitik In Vitro Ekstrak Etabolik Herba Meniran (Phyllanthus niruri L) Terhadap Mukosa Usus Sapi. Jurnal Ilmu Farmasi & Farmasi Klinik, 3, 94–100.




DOI: https://doi.org/10.14421/biomedich.2024.132.541-547

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2024 Desy Aryanti Pardilla Vitri, Siti Rahmatul Aini, Legis Ocktaviana Saputri



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC