Email this article 
Histopathological Description of Mouse Liver in a Sepsis Model Infected with Escherichia coli Treated with Paederia foetida L. Leaf Extract for Sepsis Prevention
Abstract
The leaf of Paederia foetida L. is one type of medicinal plant that can be used as a preventive medicine for sepsis. This plant contains secondary metabolites such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. The objective of this study was to determine the histopathological description of the liver in a mouse sepsis model infected with E. coli, with the administration of Paederia foetida L. leaf extract for sepsis prevention, and to ascertain the influence and effective dosage of the leaf extract as a preventive measure against liver histopathology in the sepsis model induced by E. coli. The method employed was a Completely Randomized Design (CRD). The study used 24 male white mice divided into 6 (six) groups. Data analysis was conducted using One Way ANOVA. The results of the study revealed the histopathological profile of liver cell degeneration in group PI (100mg/kg BW) at 20.79%±0.03, group PII (200mg/kg BW) at 21.63%±0.02, and group PIII (500mg/kg BW) at 9.08%±0.02. Necrosis rates were observed in group PI (100mg/kgBW) at 22.62%±0.04, group PII (200mg/kg BW) at 17.63%±0.02, and group PIII (500mg/kg BW) at 6.05%±0.02. The presence of polymorphonuclear leukocytes (PMN) was detected in group PI (100mg/kgBW) at 39.56%±0.03, group PII (200mg/kgBW) at 28.05%±0.02, and group PIII (500mg/kg BW) at 18.45%±0.03. The test results showed a significant effect of P. foetida L. leaf extract as a preventive measure against liver histopathology in the mouse sepsis model infected with E. coli, with significant values for necrosis (p=0.000), cell degeneration (p=0.000), and PMN (p=0.000). The most effective dosage of P. foetida L. leaf extract as a preventive measure against liver histopathology in the mouse sepsis model infected with E. coli was the dosage used in group PIII (500mg/kgBW).
Keywords
Full Text:
PDFReferences
Angus, D. C., & Poll, T. Van Der. (2015). Severe sepsis and septic shock. The New England Journal of Medicine, 369(9), 840–851. https://doi.org/10.1056/NEJMRA1208623
Bai, R., Yao, C., Zhong, Z., Ge, J., Bai, Z., Ye, X., Xie, T., & Xie, Y. (2021). Discovery of natural anti-inflammatory alkaloids: Potential leads for the drug discovery for the treatment of inflammation. European Journal of Medicinal Chemistry, 213(33), 574–785. https://doi.org/10.1016/J.EJMECH.2021.113165
Chen, P. Y., Liao, Y. H., Huang, W. T., Lin, Y. C., & Hou, Y. Te. (2023). Effects of tannic acid on liver function in a small hepatocyte–based detachable microfluidic platform. Biochemical Engineering Journal, 190(21), 108–120. https://doi.org/10.1016/J.BEJ.2022.108757
Dwiridal, H. P., Adrial, A., & Darwin, E. (2021). Gambaran Histopatologi Hepar Mencit (Mus musculus Balb/C) yang Diinfeksi dengan Plasmodium berghei. Jurnal Ilmu Kesehatan Indonesia, 2(2), 1–8. https://doi.org/10.25077/JIKESI.V2I2.258
Fikru, A., Makonnen, E., Eguale, T., Debella, A., & Abie Mekonnen, G. (2012). Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L. Journal of Ethnopharmacology, 143(2), 469–474. https://doi.org/10.1016/j.jep.2012.06.049
Ghozali, I. (2009). Aplikasi Analisis Multivariate Dengan Program SPSS (4th ed.). Universitas Diponegoro.
Jarczak, D., Kluge, S., & Nierhaus, A. (2021). Sepsis-Pathophysiology and Therapeutic Concepts. Frontiers in Medicine, 8. https://doi.org/10.3389/FMED.2021.628302
Kemenkes RI. (2017). Pedoman Nasional Pelayanan Kedokteran Tata Laksana Sepsis. Kementerian Kesehatan Rebublik Indonesia.
Maleki, S. J., Crespo, J. F., & Cabanillas, B. (2019). Anti-inflammatory effects of flavonoids. Food Chemistry, 299(112), 1254–1266. https://doi.org/10.1016/J.FOODCHEM.2019.125124
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: an overview. Journal of Nutritional Science, 5(3), 1–15. https://doi.org/10.1017/JNS.2016.41
Purwanto, D. S., & Astrawinata, D. A. W. (2018). Mekanisme Kompleks Sepsis dan Syok Septik. Jurnal Biomedik:JBM, 10(3), 143–151. https://doi.org/10.35790/JBM.10.3.2018.21979
Salamah, K. Z., & Halim, G. (2021). Uji Efektivitas Antidiare Ekstrak Etanol 96% Daun Sembukan (Paederia Foetida L.) Pada Mencit (Mus Musculus) Yang Diinduksi Bakteri Escherichia Coli. INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL, 6(2), 10–19. https://doi.org/10.52447/INRPJ.V6I2.5282
Silaban, H. (2021). The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria. Journal of Drug Delivery and Therapeutics, 11(6), 61–67. https://doi.org/10.22270/JDDT.V11I6.5037
Singer, M., Deutschman, C. S., Seymour, C., & Shankar-Hari, M. (2016). The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA, 315(8), 810. https://doi.org/10.1001/JAMA.2016.0287
Tan, P., Jin, L., Qin, X., & He, B. (2022). Natural flavonoids: Potential therapeutic strategies for non-alcoholic fatty liver disease. Frontiers in Pharmacology, 13(17), 166–178. https://doi.org/10.3389/FPHAR.2022.1005312
Waheed, A., Barker, J., Barton, S. J., Owen, C. P., Ahmed, S., & Carew, M. A. (2012). A novel steroidal saponin glycoside from Fagonia indica induces cell-selective apoptosis or necrosis in cancer cells. European Journal of Pharmaceutical Sciences: Official Journal of the European Federation for Pharmaceutical Sciences, 47(2), 464–473. https://doi.org/10.1016/J.EJPS.2012.07.004
Wang, W., Liu, Y., Sun, M., Sai, N., You, L., Dong, X., Yin, X., & Ni, J. (2019). Hepatocellular Toxicity of Paris Saponins I, II, VI and VII on Two Kinds of Hepatocytes-HL-7702 and HepaRG Cells, and the Underlying Mechanisms. Cells, 8(7), 1445–1458. https://doi.org/10.3390/CELLS8070690
WHO. (2021). Sepsis. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/sepsis
DOI: https://doi.org/10.14421/biomedich.2024.132.485-492
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Lisa Savitri, Ana Retnowati, Elfred Rinaldo Kasimo, Rochmad Krissanjaya, Syntia Tanu Juwita
Biology, Medicine, & Natural Product Chemistry |
|
