Analysis of Nutritional Composition and Antioxidant Activity of Yogurt Enriched with Butterfly Pea (Clitoria ternatea L.) Extract

Yulia Windarsih, I Made Budiarsa, Manap Trianto, Akram Akram, Abdul Ashari

Abstract


The growing demand for functional foods has encouraged the development of dairy products enriched with natural bioactive ingredients to improve nutritional value and health benefits. This study aimed to analyze the nutritional composition and antioxidant activity of yogurt enriched with butterfly pea (Clitoria ternatea L.) extract. Yogurt was prepared from fresh goat milk with varying concentrations of butterfly pea extract and evaluated for protein, fat, carbohydrate, moisture, ash, vitamin C contents, antioxidant activity, and sensory acceptance. Proximate analysis, UV–Vis spectrophotometry, and the DPPH method were used for chemical determinations, while a hedonic test assessed color, taste, aroma, and texture. Results showed that increasing butterfly pea extract significantly decreased protein and fat contents but increased vitamin C and antioxidant activity, with the highest values observed in yogurt containing 60% extract and 4% formulation (N60K2). Moisture and ash contents were not significantly affected. Sensory evaluation indicated that yogurt with 60% extract and 2% formulation (N60K1) was preferred regarding color, taste, aroma, and texture. Considering compliance with the Indonesian National Standard (SNI) and consumer acceptance, N60K1 was the optimal formulation. The findings suggest that butterfly pea extract can enhance the functional properties of yogurt, particularly its antioxidant potential, while maintaining acceptable sensory quality. This provides insights for developing functional dairy products with natural plant-based bioactives.

Keywords


Antioxidant Activity; Butterfly Pea; Clitoria ternatea; Nutritional Composition; Yogurt

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Akalu, Z. K., & Geleta, S. H. (2019). Comparative analysis on the proximate composition of tubers of Colocasia esculenta L. Schott and Dioscorea alata cultivated in Ethiopia. American Journal of Bioscience and Bioengineering, 7(6), 93–101.

Al-Bedrani, D. I., Alkaisy, Q. H., & Rahi, A. K. (2023). Evaluation of milk source on physicochemical, texture, rheological and sensory properties of yogurts. Journal of Applied and Natural Science, 15(1), 128.

Ali, M. A., Kamal, M. M., Rahman, M. H., Siddiqui, M. N., Haque, M. A., Saha, K. K., & Rahman, M. A. (2022). Functional dairy products as a source of bioactive peptides and probiotics: Current trends and future prospectives. Journal of Food Science and Technology, 59(4), 1263–1279.

Arab, M., Yousefi, M., Khanniri, E., Azari, M., Ghasemzadeh-Mohammadi, V., & Mollakhalili-Meybodi, N. (2023). A comprehensive review on yogurt syneresis: Effect of processing conditions and added additives. Journal of Food Science and Technology, 60(6), 1656–1665.

Arain, M. A., Rasheed, S., Jaweria, A., Khaskheli, G. B., Barham, G. S., & Ahmed, S. (2023). A review on processing opportunities for the development of camel dairy products. Food Science of Animal Resources, 43(3), 383.

Bankole, A. O., Irondi, E. A., Awoyale, W., & Ajani, E. O. (2023). Application of natural and modified additives in yogurt formulation: Types, production, and rheological and nutraceutical benefits. Frontiers in Nutrition, 10, 1257439.

Brodziak, A., Król, J., Bar?owska, J., Teter, A., & Florek, M. (2020). Changes in the physicochemical parameters of yoghurts with added whey protein in relation to the starter bacteria strains and storage time. Animals, 10(8), 1350.

Chalid, S. Y., Kinasih, P. N., Hatiningsih, F., & Rudiana, T. (2021). Antioxidant activities and profile of amino acid of yoghurt from beef milk fermentation with dadih starter. Jurnal Kimia Valensi, 7(1), 58–68.

De Mejia, E. G., Zhang, Q., Penta, K., Eroglu, A., & Lila, M. A. (2020). The colors of health: Chemistry, bioactivity, and market demand for colorful foods and natural food sources of colorants. Annual Review of Food Science and Technology, 11(1), 145–182.

Farag, M. A., Jomaa, S. A., Abd El-Wahed, A., & El-Seedi, H. R. (2020). The many faces of kefir fermented dairy products: Quality characteristics, flavour chemistry, nutritional value, health benefits, and safety. Nutrients, 12(2), 346.

Gan, J., Kong, X., Wang, K., Chen, Y., Du, M., Xu, B., ... & Yu, T. (2023). Effect of fermentation using different lactic acid bacteria strains on the nutrient components and mineral bioavailability of soybean yogurt alternative. Frontiers in Nutrition, 10, 1198456.

Giannakourou, M. C., & Taoukis, P. S. (2021). Effect of alternative preservation steps and storage on vitamin C stability in fruit and vegetable products: Critical review and kinetic modelling approaches. Foods, 10(11), 2630.

Gulcin, ?. (2020). Antioxidants and antioxidant methods: An updated overview. Archives of Toxicology, 94(3), 651–715.

Gyawali, R., Feng, X., Chen, Y. P., Lorenzo, J. M., & Ibrahim, S. A. (2022). A review of factors influencing the quality and sensory evaluation techniques applied to Greek yogurt. Journal of Dairy Research, 89(2), 213–219.

Habiba, M. U., Augustin, M. A., Varela, C., Morris, H., Rahman, M. M., & Bozkurt, H. (2025). Probiotic dairy innovations: Exploring buffalo milk potential for food product development. Comprehensive Reviews in Food Science and Food Safety, 24(4), e70236.

Handayani, L., Aprilia, S., Arahman, N., & Bilad, M. R. (2024). Identification of the anthocyanin profile from butterfly pea (Clitoria ternatea L.) flowers under varying extraction conditions: Evaluating its potential as a natural blue food colorant and its application as a colorimetric indicator. South African Journal of Chemical Engineering, 49(1), 151–161.

Hasanah, N. N., Mohamad Azman, E., Rozzamri, A., Zainal Abedin, N. H., & Ismail-Fitry, M. R. (2023). A systematic review of butterfly pea flower (Clitoria ternatea L.): Extraction and application as a food freshness pH-indicator for polymer-based intelligent packaging. Polymers, 15(11), 2541.

Ikrawan, Y., Rukmana, J., Yelliantty, Hariadi, H., Hidayat, & Rahmawati, L. (2023). Effect of trehalose and butterfly pea (Clitoria ternatea L.) on physicochemical characteristics of drum dried milk powder. Food Science and Technology, 43, e118622.

Islam, S. R., Tanzina, A. Y., Foysal, M. J., Hoque, M. N., Rumi, M. H., Siddiki, A. Z., ... & Mannan, A. (2021). Insights into the nutritional properties and microbiome diversity in sweet and sour yogurt manufactured in Bangladesh. Scientific Reports, 11(1), 22667.

Jena, R., & Choudhury, P. K. (2025). Bifidobacteria in fermented dairy foods: A health beneficial outlook. Probiotics and Antimicrobial Proteins, 17(3), 1–22.

Jeyaraj, E. J., Lim, Y. Y., & Choo, W. S. (2021). Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. Journal of Food Science and Technology, 58(6), 2054–2067.

Juturu, V., & Wu, J. C. (2016). Microbial production of lactic acid: The latest development. Critical Reviews in Biotechnology, 36(6), 967–977.

Karimi, F., Hamidian, Y., Behrouzifar, F., Mostafazadeh, R., Ghorbani-HasanSaraei, A., Alizadeh, M., ... & Asrami, P. N. (2022). An applicable method for extraction of whole seeds protein and its determination through Bradford's method. Food and Chemical Toxicology, 164, 113053.

Kaur, R., Kaur, G., Mishra, S. K., Panwar, H., Mishra, K. K., & Brar, G. S. (2017). Yogurt: A nature's wonder for mankind. International Journal of Fermented Foods, 6(1), 57–69.

Kok, C. R., & Hutkins, R. (2018). Yogurt and other fermented foods as sources of health-promoting bacteria. Nutrition Reviews, 76(Suppl. 1), 4–15.

Kumar, S. A., Negi, A., Santhoshkumar, P., Moses, J. A., & Sinija, V. R. N. (2025). Coconut: Expanding avenues in processing and an exposition on non?conventional value?added products. Journal of the Science of Food and Agriculture, 105(3), 1522–1532.

Lakshan, S. A. T., Jayanath, N. Y., Abeysekera, W. P. K. M., & Abeysekera, W. K. S. M. (2019). A commercial potential blue pea (Clitoria ternatea L.) flower extract incorporated beverage having functional properties. Evidence?Based Complementary and Alternative Medicine, 2019(1), 2916914.

Li, B., Ye, L., Zhao, Y., Liu, Y., Chen, Y., & Zhang, H. (2025). A comprehensive review of probiotic yogurt: Nutritional modulation, flavor improvement, health benefits, and advances in processing techniques. Agricultural Products Processing and Storage, 1(1), 17.

Li, H., Gao, J., Chen, W., Qian, C., Wang, Y., Wang, J., & Chen, L. (2022). Lactic acid bacteria isolated from Kazakh traditional fermented milk products affect the fermentation characteristics and sensory qualities of yogurt. Food Science & Nutrition, 10(5), 1451–1460.

Li, N., Yang, M., Guo, Y., Tong, L. T., Wang, Y., Zhang, S., ... & Liu, L. (2022). Physicochemical properties of different pea proteins in relation to their gelation ability to form lactic acid bacteria induced yogurt gel. LWT, 161, 113381.

Li, W., O’Kelly, B. C., Fang, K., & Yang, M. (2022). Briefing: Water content determinations of peaty soils using the oven-drying method. Environmental Geotechnics, 9(1), 3–11.

Marpaung, A. M., Lee, M., & Kartawiria, I. S. (2020). The development of butterfly pea (Clitoria ternatea) flower powder drink by co-crystallization. Indonesian Food Science and Technology Journal, 3(2), 34–37.

Maulid, D. Y., Nusaibah, N., Siregar, A. N., Idris, M. H., & Yuniarti, E. (2025). The effect of fermentation time on the chemical and sensory characteristics of yoghurt containing Eucheuma cottonii seaweed. G-Tech: Jurnal Teknologi Terapan, 9(1), 474–481.

Mefleh, M., Darwish, A. M. G., Mudgil, P., Maqsood, S., & Boukid, F. (2022). Traditional fermented dairy products in southern Mediterranean countries: From tradition to innovation. Fermentation, 8(12), 743.

Mehwish, A. U., Manzoor, M., Aleem, K., Shafi, F., Shamoon, F., & Rani, A. (2023, April). Rohail (2023) A review exploring the incorporation of microalgae to enhance the nutritional profile and health benefits of dairy products. Agrobiol Rec, 13, 92–100.

Milovanovic, B., Djekic, I., Miocinovic, J., Djordjevic, V., Lorenzo, J. M., Barba, F. J., ... & Tomasevic, I. (2020). What is the color of milk and dairy products and how is it measured? Foods, 9(11), 1629.

Molina, C. V., Lima, J. G., Moraes, I. C., & Pinho, S. C. (2019). Physicochemical characterization and sensory evaluation of yogurts incorporated with beta-carotene-loaded solid lipid microparticles stabilized with hydrolyzed soy protein isolate. Food Science and Biotechnology, 28(1), 59–66.

Oliveira, S. M., Brandao, T. R., & Silva, C. L. (2016). Influence of drying processes and pretreatments on nutritional and bioactive characteristics of dried vegetables: A review. Food Engineering Reviews, 8(2), 134–163.

Parniakov, O., Apicella, E., Koubaa, M., Barba, F. J., Grimi, N., & Lebovka, N. (2016). Ultrasound-assisted green solvent extraction of high?added value compounds from microalgae Nannochloropsis spp. Bioresource Technology, 198, 262–267.

Patel, S., & Prajapati, J. B. (2013). Food and health applications of exopolysaccharides produced by lactic acid bacteria. Advances in Dairy Research, 1(2), 1–8.

Patel, S., Majumder, A., & Goyal, A. (2012). Potentials of exopolysaccharides from lactic acid bacteria. Indian Journal of Microbiology, 52(1), 3–12.

Patil, S., & Jadhav, V. V. (2020). A review on natural food colorants. Journal of Pharmacognosy and Phytochemistry, 9(3), 196–200.

Pereira, A. L. F., Maciel, T. C., & Rodrigues, S. (2011). Probiotic beverage from cashew apple juice fermented with Lactobacillus casei. Food Research International, 44(5), 1276–1283.

Pescuma, M., Hébert, E. M., Mozzi, F., & Font de Valdez, G. (2015). Functional fermented whey?based beverage using lactic acid bacteria. International Journal of Food Microbiology, 194, 40–48.

Pratama, F., & Dwivany, F. M. E. (2021). Natural blue pigments from butterfly pea (Clitoria ternatea L.) flower petal extract. IOP Conference Series: Earth and Environmental Science, 755(1), 012050.

Putra, A. A., Astuti, R. T., & Yunianta, Y. (2020). Antioxidant activity of butterfly pea (Clitoria ternatea L.) extract in yogurt drink during cold storage. Jurnal Pangan dan Agroindustri, 8(2), 89–96.

Rizqiati, H., Bintari, S. H., & Lestari, D. (2017). Karakteristik fisik dan sifat organoleptik yoghurt drink dengan penambahan sari buah naga merah (Hylocereus polyrhizus). Jurnal Aplikasi Teknologi Pangan, 6(3), 111–115.

Rosida, R., Sari, E. N., & Kurniawan, D. (2021). Physical and chemical properties of yogurt from soy milk with addition of butterfly pea flower extract (Clitoria ternatea L.). IOP Conference Series: Earth and Environmental Science, 653(1), 012054.

Rosyidi, D., Hidayat, M. N., & Nurwantoro, N. (2022). Quality characteristics of goat milk yogurt enriched with purple sweet potato (Ipomoea batatas L.) extract. Jurnal Ilmu Ternak, 22(2), 111–118.

Saepudin, E., & Rachmawati, D. (2020). Karakteristik yoghurt dengan substitusi susu kedelai dan penambahan sari buah naga (Hylocereus polyrhizus). Jurnal Ilmu dan Teknologi Pangan, 5(1), 1–8.

Sari, D. P., Agustina, W., & Fitriyani, R. (2020). Physical, chemical, and sensory characteristics of yogurt enriched with black rice (Oryza sativa L.) extract. Jurnal Teknologi Hasil Pertanian, 13(1), 1–10.

Septiani, R., Sutrisno, S., & Sumarni, S. (2017). Pengaruh lama fermentasi terhadap sifat fisik dan kimia yoghurt susu kambing. Jurnal Ilmu Peternakan Terapan, 1(1), 15–21.

Shori, A. B. (2016). Influence of food matrix on the viability of probiotic bacteria: A review based on dairy and non?dairy beverages. Food Bioscience, 13, 1–8.

Sipayung, R. E., & Sinaga, M. (2020). Karakteristik fisikokimia dan organoleptik yoghurt susu sapi dengan penambahan sari buah naga (Hylocereus polyrhizus). Jurnal Teknologi dan Industri Pangan, 31(2), 101–110.

Soomro, A. H., Masud, T., & Anwaar, K. (2002). Role of lactic acid bacteria (LAB) in food preservation and human health – A review. Pakistan Journal of Nutrition, 1(1), 20–24.

Sornplang, P., & Piyadeatsoontorn, S. (2016). Probiotic isolates from unconventional sources: A review. Journal of Animal Science and Technology, 58(1), 26.

Suharyono, S., & Wulandari, D. (2021). Pengaruh penambahan ekstrak bunga telang (Clitoria ternatea) terhadap mutu fisik, kimia dan organoleptik yoghurt. Jurnal Teknologi Pertanian, 22(3), 211–220.

Sutaryo, T., & Fitriani, E. (2018). Kualitas yoghurt dengan penambahan sari buah naga merah (Hylocereus polyrhizus). Jurnal Teknologi Hasil Pertanian, 11(2), 83–90.

Syam, N., & Widowati, S. (2017). Karakteristik fisikokimia dan organoleptik yoghurt drink dengan penambahan sari buah naga (Hylocereus polyrhizus). Agritech, 37(1), 53–58.

Tamime, A. Y., & Robinson, R. K. (2007). Yoghurt: Science and technology (3rd ed.). Woodhead Publishing.

Vasileva, I., Petkova, N., & Denev, P. (2016). Total phenolic content, antioxidant activity and chemical composition of industrially produced tomato powders. Journal of Food Processing and Preservation, 40(6), 1194–1201.

Wulandari, A., & Susanto, W. H. (2014). Pengaruh penambahan sari buah naga merah (Hylocereus polyrhizus) terhadap karakteristik yoghurt drink. Jurnal Pangan dan Agroindustri, 2(3), 121–130.

Yuliana, N. D., & Saputra, R. (2019). Pengaruh penambahan sari buah naga merah (Hylocereus polyrhizus) terhadap sifat organoleptik yoghurt drink. Jurnal Ilmiah Teknosains, 5(2), 135–142.

Zioud, A., Alkhatib, H. S., & Al?Hroub, H. A. (2017). Formulation and evaluation of flavored probiotic yogurt using natural ingredients. International Journal of Advanced Research, 5(1), 1874–1884.




DOI: https://doi.org/10.14421/biomedich.2025.142.831-841

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Copyright (c) 2025 Yulia Windarsih, I Made Budiarsa, Manap Trianto, Akram, Abdul Ashari



Biology, Medicine, & Natural Product Chemistry
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