In Vivo Evaluation of Antidepressant Potential of Temulawak (Curcuma xanthorriza Robb.) Ethanol Extract in Mus musculus

Raja David Virgilius, Zest Lively Limbong, Jericho L. Ginting

Abstract


Temulawak (Curcuma xanthorriza Roxb.) is a medicinal plant from the Zingiberceae family widely used in Indonesia for its various bioactives, including antiviral, antibacterial, and hepatoprotective properties. This research aims to evaluate the antidepressant potential of Curcuma xanthorriza ethanol extract in Mus musculus through in vivo testing. The extraction used the maceration method with 96% ethanol as a solvent, yielding 5.2504% extract. The primary bioactive compounds identified in the extract were terpenoids and curcuminoids, which are known for their neuroprotective and antidepressant effects. The antidepressant activity was assessed using the tail-suspension test (TST) and forced-swimming test (FST), with immobility time as the main parameter. Amitriptyline, a standard antidepressant, was used as a positive control, while sodium carboxymethylcellulose served as a negative control. The results showed that increasing doses of Curcuma xanthorriza extract significantly reduced immobility time, indicating an antidepressant-like effect. Statistical analysis revealed that the immobility time in mice treated with a 650 mg/Kg BW dose of Curcuma xanthorriza extract was not significantly different from the amitriptyline group, suggesting comparable efficacy. These findings highlight the potential of Curcuma xanthorriza ethanol extract as a natural antidepressant, warranting further investigation into its mechanisms and clinical applications.

Keywords


Antidepressant; Curcuma xanthorriza Roxb.; Forced-swimming test Phytochemistry; Tails-suspension test

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References


Abin M., Abhilash VA., A Karunaya., Piyush KJ., Alain LB., & Ashish R. (2024). Conventional vs modern extraction techniques in the food industry. Extraction Processes in the Food Industry, 20(5), 97-146. doi: 10.1016/B978-0-12-819516-1.00013-2

Aisyah TS., & Erminawati W. (2022). Effect of ethanol concentration extraction time with microwave assisted extraction on antioxidant activity of temulawak-extract (Curcuma xanthorriza Roxb.). Journal of Functional Food and Nutraceutical, 3(2), 63-69.

Castagne V., Moser P., Roux S., & Porsold RD. (2010). Rodent models of depression: forced swim and tail suspension behavioral despair tests in rats and mice. Current Protocols in Pharmacology, 49(1), 1-14. doi: 10.1002/0471141755.ph0508s49.

Chand, S. P., & Arif, H. (2023). Depression. In StatPearls. StatPearls Publishing.

Charlie K., Hafiz A., Linda A., & Daniel C. (2024). Laporan kasus: Gangguan depresi mayor pada mahasiswa fakultas kedokteran. Jurnal Pranata Biomedika. 3(1), 32-42. ISSN: 2828-1233

Chittaranjan A., & Sanjay KR. (2010). How antidepressant drugs act: a primer neuroplasticity as the eventual mediator of antidepressant efficacy. Indian Journal of Psychiatry. 52(4), 378-386. doi: 10.4103/0019-5545.74318.

Handayani D., Munim A., & Ranti AS. (2014). Optimation of green tea waste extraction using microwave assisted extraction to yield green tea extract. Traditional Medicine Journal, 19(1), 29-35.

Henny SW., Sri Y., Hanifah SA., & Devi R. (2019). Characterization of synthesized sodium carboxymethylcellulose with variation of solvent mixture and alkali concentration. Open Access Macedonian Journal of Medical Sciences, 7(22), 3878-3881. doi: 10.3889/oamjms.2019.524.

Kim L. (2017). A brief review of the pharmacology of amitriptyline and clinical outcomes in treating fibromyalgia. Biomedicines, 5(2), 20-28. doi: 10.3390/biomedicines5020024

Li Y., Li S., Lin S., Zhang J., Zhao C., & Li H. (2017). Microwave-assisted extraction of natural antioxidants from the exotic Gordonia axillaris fruit: Optimization and identification of phenolic compounds. Molecules. 22, 1481-1495.

Lidvina NY., Matea ND., Vicha PK., Putri TRW., Feranita EP., Melania O., & Dewi S. (2023). Review: extraction of temulawak rhizomes (Curcuma xanthorriza Roxb.) and activity as antibacterial. Jurnal Jamu Indonesia. 8(1), 10-17. doi: 10.29244/jji.v8i1.265.

Rahmat E., Lee J., & Kang Y. (2021). Javanese turmeric (Curcuma xanthorriza Roxb.): ethnobotany, phytochemistry, biotechnology, and pharmacological activities. Evidence-Based Complement Alternative Medicine. 2021(1), 9960813. doi: 10.1155/2021/9960813

Nur F., Muhaimin R., Masruri., Septian TW., & Nashi W. (2022). Potential of Curcuma xanthorriza ethanol extract in inhibiting the growth of T47D breast cancer cell line: in vitro and bioinformatics approach. Journal of Pharmacy & Pharmacognosy Research, 10 (6), 1015-1025. doi: 10.56499/jppres22.1448.10.6.1015

Shumeng LV., Guangzheng Z., Yufei H., Jiamin L., Ni Y., Yitong L., Haoteng M., Yuexiang M., & Jing T. (2023). Antidepressant pharmacological mechanism: focusing on the regulation of autophaghy. Frontiers, 14, 27-34. doi: 10.3389/fphar.2023.1287234

Syamsudin, RAMR., Perdana F.,, Mutiaz FS., Galuh V., Rina APA., Cahyani ND., Apriliya S., Yanti R., & Khendri F. (2019). Temulawak plant (Curcuma xanthorriza Roxb) as a traditional medicine. Jurnal Ilmiah Farmako Bahari, 10(1),51-65. ISSN: 2087-0337.

Ueno H., Takahashi Y., Murakami S., Wani K., Matsumoto Y., Okamoto M., & Ishihara T. (2022). Effect of simultaneous testing of two mice in the tail suspension test and forced swim test. Scientific Reports, 12(1), 1-14. doi: 10.1038/s41598-022-12986-9.

Yunxi Z., Xu C., Yuyang H., & Dingwen H. (2023). The application of antidepressant drugs in cancer treatment. Biomedicine and Pharmacotheraphy. 157, 113986. doi: 10.1016/j.biopha.2022.113985

Zhang CM., Fan PH., Li M., & Lou HX. (2014) Two new sesquiterpenoids from the rhizomes of Curcuma xanthorriza. Helvetica Chicima Acta. 97(9), 1295-1300. doi: 10.1002/hlca.201300440.

Zhang C., Ji J., Ji M., & Fan P. (2015). Acetylcholinesterase inhibitors and compounds promoting SIRT 1 expression from Curcuma xanthorriza. Phytochemistry Letters. 12(1), 215-219. doi: 10.1016/j.phytol.2015.04.007.




DOI: https://doi.org/10.14421/biomedich.2025.141.239-242

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Copyright (c) 2025 Raja David Virgilius, Zest Lively Limbong, Jericho L. Ginting



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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This work is licensed under a CC BY-NC