Evaluation of Antimicrobial Potential using Disc Diffusion Assay of Seagrape Macroalgae Extract (Caulerpa sp.) in the waters of Pasaran Island, Lampung as an Anti-Inflammatory Agent

Annisa Nurjanah, Eri Surya Kurniawan, Giani Giani, Deni Prasetyo, Gustiviana Shandy Pramesti, Revo Aulia Rahmando, An Syafitri, Fais Safiqul Umam, Wanda Andella Putri, Muhammad Kholiqul Amiin

Abstract


Sea grape (Caulerpa sp.) is a green seaweed species with bioactive content consisting of flavonoids, terpenoids, alkaloids, and phenols. This study evaluates the antimicrobial potential of Sea*Grape macroalgae extract (Caulerpa sp.) obtained from Pasaran Island, Lampung. The diffusion assay method assessed the antimicrobial activity of Caulerpa sp. extract against Staphylococcus aureus. Caulerpa sp. samples were extracted using the maceration extraction method and evaporated using two methods: water bath and rotary evaporator. The results showed that Caulerpa sp. extract had antimicrobial activity at a concentration of 10,000 ppm, which was resistant to Staphylococcus aureus. These findings suggest that Caulerpa sp. from the waters of Pasaran Island has the potential to be a source of natural antimicrobial and anti-inflammatory components if the concentration of the extract is more significant, which can be further developed for applications in the health and pharmaceutical fields.

Keywords


Macroalgae; Inflammation; Sea Grape; Biological Agents

Full Text:

PDF

References


Achmadi I, Subekti S, Ardiyanti HB, Amiin MK, Haryanto LNF, Akbar REK and Yudarana MA (2021) Molecular identification and prevalence of ectoparasite worms in barramundi (Lates calcarifer) in Lampung Waters. IOP Conf. Ser.: Earth Environ. Sci. 679 012058. https://doi.org/10.1088/1755-1315/679/1/012058

Agustina, Ri., D. T. Indrawati, & M. A.Masruhin.

(2015). Aktivitas ekstrak daun salam (Eugenia poyantha) sebagai antiinflamsi pada Tikus Putih (Rattus norvegicus). J. Trop. Pharm. Chem, 3(2):120-123. https://doi.org/10.25026/jtpc.v3i2.96

Amiin, M.K.; Lahay, A.F. Anti-Bacterial Effectiveness of Cymodocea rotundata Extract and Assay for Primary Bioactive Composition. J. Aquatropica Asia 2023, 8, 6–12.

Ariyanti, N.K., Darmayasa, I.B.G., & Sudirga, S.K. (2012). Daya hambat ekstrak kulit daun lidah buaya (aloe barbadensis miller) terhadap pertumbuhan bakteri Staphylococcus aureus atcc 25923 dan eschericia coli atcc25922. Jurnal Biologi XVI, (1):1-4.

Arenajo, A. R., Ybañez, A. P., Anne, J., Tan, G. F., Villajuan, C. E., Young, C. P., Reyes, L. A., Fierro, E. M. Del, & Labiaga, A. S. (2018). In vitro anticoagulant potential of Caulerpa sp. (Lato), Eucheuma sp. (Guso), Ananas comosus (Pineapple) peeling and psidium guajava (guava) leaf extracts in icr mice. Journal of Agriculture and Technology Management, 21(1): 30–34.

Darmawati, D. (2017). Kajian pertumbuhan dan kualitas rumput laut Caulerpa sp. yang dibudidayakan pada kedalaman dan jarak tanam berbeda: kajian prospek pengembangan budidaya: the study of the growth and quality of seaweed Caulerpa sp.cultivated on different planting distance. Disertasi Thesis. Universitas Hasanuddin. repository.unhas.ac.id:443/id/eprint/29712.

Darmawati, Niartiningsih, A., Syamsuddin, R., & Jompa, J. (2016). Analisis Kandungan Karotenoid Rumput Laut Caulerpa sp. yang Dibudidayakan di Berbagai Jarak dan Kedalaman. Prosiding Inovasi IPTEKS Perguruan Tinggi Untuk Meningkatkan Kesejahteraan Masyarakat. hlm 196–201.

DiazGranadoz, C., Cardo, D., & McGowan, J. (2008). Antimicrobial resistance: international control strategies, with a focus on limited-resource settings, Int. Antimicroba Agents, 32(1): 1-9.

Fernandez, B. A. (2013). Studi pengunaan antibiotik tanpa resep di Kabupaten Manggarai dan Manggarai Barat - NTT. Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2(2): 1-17.

Frieri, M., Kumar, K., & Boutin, A. (2017). Antibiotic resistance. Journal of infection and public health, 10(4), 369-378. doi.org/10.1016/j.jiph.2016.08.007.

Ines Septiyaningrum, Maya A. F. U., dan Yar Johan. (2020). Identifikasi jenis anggur laut (Caulerpa sp.) Teluk Sepang Kota Bengkulu. Jurnal Perikanan, 10(2): 195-204. doi.org/10.29303/jp.v10i2.215.

Julyasih, K. S. M., & Wirawan, I. G. P. (2017). Potential effect of macro alga Caulerpa sp. and Gracilaria sp. extract lowering malondialdehyde level of wistar rats fed high cholesterol diet. International Journal of Biosciences and Biotechnology, 5(1): 71. doi.org/10.24843/ijbb.2017.v05.i01.p06.

Lahay AF & Amiin MK (2023) Antibacterial Potential of Seagrass Cymodocea Rotundata (Alismatales: Cymodoceaceae) Extract on The Pathogenic Bacteria Staphylococcus Aureus. Jurnal Biologi Tropis, 23(2): 355–360.

Latief, M., Amanda, H., Utami, A., Muhaimin, & Nurhayati. (2019). Isolation of active compounds from the leaf extract of patah kemudi (Abroma augusta L.) and its anti-inflammatory activity. Journal of Physics: Conference Series, 1282(1): 1–8.doi.org/10.1088/17426596/1282/1/012064.

Lewis, J. S. ., Weinstein, M. P. ., Bobenchik, A. M. ., Cameau, Shelley., Cullen, S. K. ., Dingle, Tanis., Galas, M. F. ., Humphries, R. M. ., Kirn, T. J. ., Limbago, Brandi., Mathers, A. J. ., Pierce, V. M. ., Richter, S. S. ., Satlin, Michael., Schuetz, A. N. ., Sharp, Susan., & Simner, Patricia. (2023). M100 : Performance Standards For Antimicrobial Susceptibility Testing. Clinical and Laboratory Standards Institute.

Marfuah, I., Dewi, E. N., & Rianingsih, L. (2018). Kajian potensi ekstrak anggur laut (Caulerpa racemosa) sebagai antibakteri terhadap bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 7(1), 7-14.

Narande J. M., Anne W., dan Adithya Y. (2013). Uji efek antiinflamsi ekstrak etanol daun suji (Dracaena angustifolia Roxb) Terhadap edema kaki tikusputih jantan galur wistar. Pharmacon, 2(3): 14-18. doi.org/10.35799/pha.2.2013.2317.

Nome, W., Salosso, Y., & Eoh, B. C. (2019). Analisis metabolit sekunder dan kandungan nutrisi dari makroalga hijau (Chlorophyceae) di Perairan Teluk Kupang. Jurnal Aquatik, 2(1): 100–112. DOI: https://doi.org/10.35508/aquatik.v2i1.2526.

Palaniyappan, S., Sridhar, A., Kari, Z. A., Téllez-Isaías, G., & Ramasamy, T. (2023). Evaluation of phytochemical screening, pigment content, in vitro antioxidant, antibacterial potential and GC-MS metabolite profiling of green seaweed Caulerpa racemosa. Marine Drugs, 21(5), 278. doi.org/10.3390/md21050278.

Pérez, M., Falqué, E., & Domínguez, H. (2016). Antimicrobial action of compounds from marine seaweed. Marine Drugs, 14(3): 52.

Ridhowati, S & Asnani. 2016. Potensi Anggur Laut Kelompok Caulerpa racemosa Sebagai Kandidat Sumber Pangan Fungsional Indonesia. Oseana Majalah Ilmiah Semi Populer, 41(4): 50 - 62.

Rusli, A., Metusalach, Tahir, M. M., Salengke, & Syamsuar. (2016). Analysis of bioactive compounds of Caulerpa recemosa, sargassum sp. And gracillaria verrucosa using different solvents. Jurnal Teknologi, 78(4–2): 15–19. doi.org/https://doi.org/10.11113/jt.v78.8146.

Saputri, A. U., Purnamayati, L., & Anggo, A. D. (2019). Aktivitas antibakteri anggur laut (Caulerpa lentillifera) terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Ilmu Dan Teknologi Perikanan, 1(1): 15–20. /doi.org/10.14710/jitpi.2019.5240.

Siagian, K.D., Daniel, L., Sepriyanto,D., & Eva, S. . (2018). Uji aktivitas antifungi anggur laut (Caulerpa sp.) asal Pulau Ambai Serui terhadap fungi Candida krusei dan Candida albicans. Pharmaceutical Journal of Indonesia, 15(01): 179–184. DOI: 10.30595/pharmacy.v15i1.3018.

Subekti S, Amin MK, Kismiyati K. (2019). Prevalence of trematodes on red snapper (Lutjanus argentimaculatus) in floating net cages at Lampung, Indonesia. Indian Vet. J, 96(2):46–48.

Tapotubun, A. M., Matrutty, T. E. A. A., Riry, J., Tapotubun, E. J., Fransina, E. G., Mailoa, M. N., Riry, W. A., Setha, B., & Rieuwpassa, F. (2020). Seaweed Caulerpa sp position as functional food. IOP Conference Series: Earth and Environmental Science, 517(1). doi.org/10.1088/1755-1315/517/1/012021.

Utami, T. P., Hanna, C., dan Miftah, I. (2021). Potensi farmakologi makroalga Genus Caulerpa bagi pengembangan obat bahan alam. Jurnal Ilmiah Farmasi Attamru (JIFA), 2(2), 37-47. doi.org/10.31102/attamru.2021.2.2.37-47.

Utami, E. R. (2012). Antibiotika, resistensi, dan rasionalitas terapi. Saintis, 1(1): 124-138.

Waji RA & Sugrani A. (2009). Makalah Kimia Organik Bahan Alam Flavonoid (Quercetin). Makasar: Universitas Hasanuddin.

Wang, Q., Jin, J., Dai, N., Han, N., Han, J., & Bao, B. (2016). Anti-inflammatory effects, nuclear magnetic resonance identification, and high-performance liquid chromatography isolation of the total flavonoids from Artemisia frigida. Journal of Food and Drug Analysis, 24(2): 385–391. doi.org/10.1016/j.jfda.2015.11.004.




DOI: https://doi.org/10.14421/biomedich.2025.141.17-24

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2025 Annisa Nurjanah, Eri Surya Kurniawan, Giani Giani, Deni Prasetyo, Gustiviana Shandy Pramesti, Revo Aulia Rahmando, An Syafitri, Fais Safiqul Umam, Wanda Andella Putri, Muhammad Kholiqul Amiin



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC