Modulatory Efficiency of Vitamin C (AscoraModulatory Efficiency of Vitamin C (Ascorbic Acid) on Collagen-Induced Platelet Aggregation and Dysfunctionbic Acid) on Collagen-Induced Platelet Aggregation and Dysfunction

Osah Martins Onwuka, Chukwukadibie Adigwe, Oghenefego Michael Adheke, Chamberlin Jamike Elem, Josiah Soipiriala Hart

Abstract


Platelet aggregation, coagulation, and activation are crucial for hemostasis. Collagen treatment can impair hemostatic processes leading to bleeding disorders like thrombosis; vitamin C may mitigate these effects.  Hence, modulatory efficiency of vitamin C on collagen-induced platelet aggregation and dysfunction was investigated. Thirty (30) Wistar rats (135g-155g) were divided equally into; Group 1 (Control), Group 2 (Collagen-induced), and Group 3 (Collagen + Vitamin C treated). Platelet aggregation, prothrombin time, bleeding time, fibrinogen levels assessed coagulation and platelet function. Thromboxane B2 and P-selectin levels measured platelet and endothelial activation. Platelet count, mean platelet volume (MPV), platelet distribution width (PDW), and plateletcrit (PCT) evaluated platelet production and size variability. Statistical significance was set at p < 0.05. Group 2 exhibited higher platelet aggregation, prolonged prothrombin and bleeding times and elevated fibrinogen, thromboxane B2, and P-selectin levels, along with increased platelet count, MPV, PDW, and PCT, compared to Group 1. Group 3 showed significant reductions in all these parameters compared to Group 2 (p < 0.05). Vitamin C demonstrated significant modulatory effect on collagen-induced platelet aggregation and dysfunction which suggests that vitamin C may have therapeutic potential in mitigating platelet dysfunction and coagulation impairments associated with collagen-induced pathophysiological conditions.

Keywords


Platelets aggregation; Collagen-induced; Vitamin C

Full Text:

PDF

References


Ashor AW, Lara J, Mathers JC, Siervo M. Effect of vitamin C on endothelial function in health and disease: a systematic review and meta-analysis of randomised controlled trials. Atherosclerosis. 2014; 235(1):9-20.

Brass LF, Newman DK, Wannemacher KM, Zhu L, Stalker TJ. Signal transduction during platelet plug formation. Platelets. 2013; 2:319-46.

Broos K, Feys HB, De Meyer SF, Vanhoorelbeke K, Deckmyn H. Platelets at work in primary hemostasis. Blood reviews. 2011; 25(4):155-67.

Cetin MS, Cetin EH, Akdi A, Aras D, Topaloglu S, Temizhan A, Aydogdu S. Platelet distribution width and plateletcrit: novel biomarkers of ST elevation myocardial infarction in young patients. Polish Heart Journal (Kardiologia Polska). 2017; 75(10):1005-12.

Chandler AB, Earhart AD, Speich HE, Kueter TJ, Hansen J, White MM, Jennings LK. Regulation of CD40L (CD154) and CD62P (p-selectin) surface expression upon GPIIb-IIIa blockade of platelets from stable coronary artery disease patients. Thrombosis research. 2010; 125(1):44-52.

Farndale RW, Sixma JJ, Barnes MJ, De Groot PG. The role of collagen in thrombosis and hemostasis. Journal of Thrombosis and Haemostasis. 2004; 2(4):561-73.

Ferroni P, Vazzana N, Riondino S, Cuccurullo C, Guadagni F, Davi G. Platelet function in health and disease: from molecular mechanisms, redox considerations to novel therapeutic opportunities. Antioxidants & Redox Signaling. 2012; 17(10):1447-85.

Ghoshal K, Bhattacharyya M. Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis. The Scientific World Journal. 2014; 2014(1):781857.

Hosseini E, Ghasemzadeh M. Different stages of platelet adhesion to the site of vascular injury. Iranian Journal of Blood and Cancer. 2012; 4(3):133-42.

Jandrey KE. Assessment of platelet function. Journal of Veterinary Emergency and Critical Care. 2012; 22(1):81-98.

Kamal AH, Tefferi A, Pruthi RK. How to Interpret and Pursue an Abnormal Prothrombin Time, Activated Partial Thromboplastin Time, and Bleeding Time in Adults. InMayo Clinic Proceedings 2007; 7(82):864-873.

Kattula S, Byrnes JR, Wolberg AS. Fibrinogen and fibrin in hemostasis and thrombosis. Arteriosclerosis, thrombosis, and vascular biology. 2017; 37(3):e13-21.

Krishnegowda M, Rajashekaraiah V. Platelet disorders: an overview. Blood Coagulation & Fibrinolysis. 2015; 26(5):479-91.

Kroll MH, Kharghan VA. Platelets in pulmonary vascular physiology and pathology. Pulmonary circulation. 2012; 2(3):291-308.

Malik A, Rehman FU, Shah KU, Naz SS, Qaisar S. Hemostatic strategies for uncontrolled bleeding: A comprehensive update. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2021; 109(10):1465-77.

May JM, Harrison FE. Role of vitamin C in the function of the vascular endothelium. Antioxidants & redox signaling. 2013; 19(17):2068-83.

Onwuka OM, Nkpurukwe CI, Osuji AC. Comparative analysis of the impact of artificially and naturally ripened fruit on obesity-induced biochemical and hematological alterations. Gastroenterol Hepatol Open Access. 2023; 14(6):175-9.

Onwuka OM, Okerulu AL, Nwosu NC. Administration of chemically ripened banana (Musa acuminate) juice to male Wistar rats depletes blood cells via impaired hematopoiesis. Bio-Research. 2022; 20(2):1552-9.

Pehlivan FE. Vitamin C: An antioxidant agent. Vitamin C. 2017; 2:23-35.

Periayah MH, Halim AS, Saad AZ. Mechanism action of platelets and crucial blood coagulation pathways in hemostasis. International journal of hematology-oncology and stem cell research. 2017; 11(4):319.

Smyth EM. Thromboxane and the thromboxane receptor in cardiovascular disease. Clinical lipidology. 2010; 5(2):209-19.

Stissing T, Dridi NP, Ostrowski SR, Bochsen L, Johansson PI. The influence of low platelet count on whole blood aggregometry assessed by Multiplate. Clinical and Applied Thrombosis/Hemostasis. 2011; 17(6):E211-7.

Tomaiuolo M, Brass LF, Stalker TJ. Regulation of platelet activation and coagulation and its role in vascular injury and arterial thrombosis. Interventional cardiology clinics. 2017; 6(1):1.

Violi F, Pignatelli P, Basili S. Nutrition, supplements, and vitamins in platelet function and bleeding. Circulation. 2010; 121(8):1033-44.

Vizioli L, Muscari S, Muscari A. The relationship of mean platelet volume with the risk and prognosis of cardiovascular diseases. International journal of clinical practice. 2009; 63(10):1509-15.

Wilkinson IB, Megson IL, MacCallum H, Sogo N, Cockcroft JR, Webb DJ. Oral vitamin C reduces arterial stiffness and platelet aggregation in humans. Journal of cardiovascular pharmacology. 1999 Nov 1;34(5):690-3.

Wohlauer MV, Moore EE, Thomas S, Sauaia A, Evans E, Harr J, Silliman CC, Ploplis V, Castellino FJ, Walsh M. Early platelet dysfunction: an unrecognized role in the acute coagulopathy of trauma. Journal of the American College of Surgeons. 2012; 214(5):739-46.




DOI: https://doi.org/10.14421/biomedich.2024.132.609-615

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2025 Osah Martins Onwuka, Chukwukadibie Adigwe, Oghenefego Michael Adheke, Chamberlin Jamike Elem, Josiah Soipiriala Hart



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC