Anti-oxidative Effects of Butanol Seed Extract of Parkinsonia aculeata on Carbon Tetrachloride-Induced Liver Damage on Wistar Rats

Muhammad Bashiru AbdulRahman, Yusuf Gumburawa Malami, Sanusi Wara Hassan, Mansur Lawal, Waliu Temitope Adanlawo, Mansur Mohammed Birnin Kebbi, Kamaldeen Olalekan Sanusi

Abstract


Medicinal plants have protective effect because of the presence of several compounds which have different mechanism of action. This study sought to assess the anti-oxidative effects of butanol seed extract of Parkinsonia aculeata on carbon tetrachloride (CCl4)-induced liver damage on Wistar rats. The Wistar rats were put into five groups, each with six rats: Group A received a daily dosage of liquid paraffin (1ml/kg); Group B received 1ml/kg body weight of CCl4 (30% in liquid paraffin intraperitoneal); Group C, D, and E received the seed extracts at 100, 120, and 160 mg/kg every day for two weeks. Induction of CCl4 was three times a week for two weeks simultaneously with the extract to the last day. After sacrificed, the liver was harvested and homogenized, and used for further analyses. There was a significant increase (p<0.05) in the levels of superoxide dismutase and catalase in all extract treated groups compared to positive control, except the catalase levels of group treated with 160mg/kg. Similar results was observed in vitamin C, vitamin E and glutathione in rats treated with 100 and 120mg/kg of the extract. The results of this study revealed that butanol seed extract from P. aculeata has antioxidant properties and can protect Wistar rats' livers from the damaging effects of CCl4.


Keywords


antioxidant; carbon tetrachloride; liver damage; oxidative stress; Parkinsonia aculeata

Full Text:

PDF

References


Achliya, G. S., Wadodkar, S. G., & Dorle, A. K. (2004). Evaluation of hepatoprotective effect of Amalkadi Ghrita against carbon tetrachloride-induced hepatic damage in rats. Journal of Ethnopharmacology, 90(2-3), 229–232. https://doi.org/10.1016/j.jep.2003.09.037

Beckman, K. B., & Ames, B. N. (1998). The free radical theory of aging matures. Physiological Reviews, 78(2), 547–581. https://doi.org/10.1152/physrev.1998.78.2.547

Covacci, V., Torsello, A., Palozza, P., Sgambato, A., Romano, G., Boninsegna, A., Cittadini, A., & Wolf, F. I. (2001). DNA oxidative damage during differentiation of HL-60 human promyelocytic leukemia cells. Chemical Research in Toxicology, 14(11), 1492–1497. https://doi.org/10.1021/tx010021m

Hartman, P. E. (1983). Putative mutagens and carcinogens in foods. IV. Malonaldehyde (malondialdehyde). Environmental Mutagenesis, 5(4), 603–607. https://doi.org/10.1002/em.2860050409

Hassan, S. W., Salawu, K. ., Ladan, M. J., Hassan, L. G., Umar, R. A., & Fatihu, M. Y. (2010). Hepatoprotective, Antioxidant and PhytochemicalProperties of Leaf Extracts of Newbouldia Laevies. International Journal of PharmTech Research, 2(1), 573–584.

Hassan, S. W., Umar, R. A., Ebbo, A. A., & Matazu, I. . (2005). Phytochemical, antibacterial and toxicity studies of Parkinsonia aculeata (Fabaceae). Nig. J. Biochem. Mol. Biol, 20(2), 89–97.

Hewawasam, R. P., Jayatilaka, K. A. P. W., Pathirana, C., & Mudduwa, L. K. B. (2003). Protective effect of Asteracantha longifolia extract in mouse liver injury induced by carbon tetrachloride and paracetamol. The Journal of Pharmacy and Pharmacology, 55(10), 1413–1418. https://doi.org/10.1211/0022357021792

Kamalakkannan, N., & Prince, P. S. M. (2006). Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic wistar rats. Basic & Clinical Pharmacology & Toxicology, 98(1), 97–103. https://doi.org/10.1111/j.1742-7843.2006.pto_241.x

Kidd, P. M. (1997). Glutathione: Systemic Protectant Against Oxidative and Free Radical Damage.

Leite, A. C. R., Araújo, T. G., Carvalho, B. M., Silva, N. H., Lima, V. L. M., & Maia, M. B. S. (2007). Parkinsonia aculeata aqueous extract fraction: biochemical studies in alloxan-induced diabetic rats. Journal of Ethnopharmacology, 111(3), 547–552. https://doi.org/10.1016/j.jep.2006.12.032

Madrigal-Santillán, E., Madrigal-Bujaidar, E., Álvarez-González, I., Sumaya-Martínez, M. T., Gutiérrez-Salinas, J., Bautista, M., Morales-González, Á., García-Luna y González-Rubio, M., Aguilar-Faisal, J. L., & Morales-González, J. A. (2014). Review of natural products with hepatoprotective effects. World Journal of Gastroenterology, 20(40), 14787–14804. https://doi.org/10.3748/wjg.v20.i40.14787

Moscarella, S., Duchini, A., & Buzzelli, G. (1994). Lipoperoxidation, trace elements and vitamin E in patients with liver cirrhosis. European Journal of Gastroenterology & Hepatology, 6, 633–636.

Nandi, A., Yan, L.-J., Jana, C. K., & Das, N. (2019). Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases. Oxidative Medicine and Cellular Longevity, 2019, 9613090. https://doi.org/10.1155/2019/9613090

Patrick-Iwuanyanwu, K. C., Wegwu, M. ., & Ayalogu, E. O. (2007). The Protective Nature of Garlic, Ginger and Vitamin E on CCl4-Induced Hepatotoxicity in Rats. Asian Journal of Biochemistry, 2(6), 409–414. https://doi.org/10.3923/ajb.2007.409.414

Poli, G. (1993). Liver damage due to free radicals. British Medical Bulletin, 49(3), 604–620. https://doi.org/10.1093/oxfordjournals.bmb.a072634

Recknagel, R. O., Glende, E. A. J., Dolak, J. A., & Waller, R. L. (1989). Mechanisms of carbon tetrachloride toxicity. Pharmacology & Therapeutics, 43(1), 139–154. https://doi.org/10.1016/0163-7258(89)90050-8

Rutkowski, M., Gezegorczyk, K., & Paradowski, M. (1998). Colorimetric determination of vitamin C concentration in blood plasma with phosphotungstate reagent- a modification of kyaw method. Diag. Lab., 34, 243.

Rutkowski, M., Gezegorczyk, K., & Paradowski, M. (2005). Colorimetric determination of vitamin C concentration in blood plasma with phosphotungstate reagent- a modification of kyaw method. Diag. Lab., 41, 375.

Serbinova, E., Kagan, V., Han, D., & Packer, L. (1991). Free radical recycling and intramembrane mobility in the antioxidant properties of alpha-tocopherol and alpha-tocotrienol. Free Radical Biology & Medicine, 10(5), 263–275. https://doi.org/10.1016/0891-5849(91)90033-y

Venukumar, M. R., & Latha, M. S. (2002). Antioxidant activity ofcurculigo orchioides in carbon tetrachloride-induced hepatopathy in rats. Indian Journal of Clinical Biochemistry : IJCB, 17(2), 80–87. https://doi.org/10.1007/BF02867976

Wadekar, R. R., Supale, R. S., Tewari, K. M., Patil, K. S., & Jalalpure, S. S. (2008). Screening of roots of Baliospermum montanum for hepatoprotective activity against paracetamol induced liver damage in albino rats. International Journal of Green Pharmacy, 2(4), 220–223. https://doi.org/10.4103/0973-8258.44737

Yasui, K., & Baba, A. (2006). Therapeutic potential of superoxide dismutase (SOD) for resolution of inflammation. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 55(9), 359–363. https://doi.org/10.1007/s00011-006-5195-y

Zhang, H.-Y., & Wang, L.-F. (2002). Theoretical elucidation on structure-antioxidant activity relationships for indolinonic hydroxylamines. Bioorganic & Medicinal Chemistry Letters, 12(2), 225–227. https://doi.org/10.1016/s0960-894x(01)00724-7




DOI: https://doi.org/10.14421/biomedich.2022.111.83-87

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2022 Muhammad Bashiru AbdulRahman, Yusuf Gumburawa Malami, Sanusi Wara Hassan, Mansur Lawal, Waliu Temitope Adanlawo, Mansur Mohammed Birnin Kebbi, Kamaldeen Olalekan Sanusi



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC