The Ability of A Consortium of Cellulose-Degrading Bacteria In Fixing Nitrogen, Dissolving Phosphorus and Potassium, and Its Application As A Bifertilizer In Tomato Plants
Abstract
This study aims to determine the ability of cellulose-degrading bacteria to fix nitrogen, dissolve phosphorus and potassium, and assess their effectiveness as biofertilizers for tomato plants. The research method used is quantitative descriptive, involving in-vitro testing and field application with two treatments: P0 (without bacteria) and P1 (with bacteria). The results of this study indicate that cellulose-degrading bacteria possess additional capabilities in nitrogen fixation, as evidenced by a color change in the NFB medium to blue, and demonstrate the ability to solubilize phosphorus and potassium, as indicated by the formation of clear zones in the Pikovskaya and Alexandrov media. Field testing showed that cellulose-degrading bacteria were effective in tomato plants, influencing plant height parameters but not affecting leaf area or number of leaves. Based on the results of the Two-Way ANOVA analysis on the treatments conducted, there was no significant effect, as the P-value was >0.005. Cellulose-degrading bacteria in vitro have the ability to provide nutrients. However, they are not yet effective in enhancing the vegetative growth of tomato plants.
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Afiati, I., & Purnamasari, R. T. (2019). Pengaruh Pemberian Bakteri Endofit Terhadap Pertumbuhan Dan Hasil Tanaman Terung Ungu (Solanum Melongena L.). Jurnal Agroteknologi Merdeka Pasuruan, 3(1), 32–37.
Afifi, L. N., Wardiyati, T., & ... (2018). Respon Tanaman Tomat (Lycopersicum esculentum Mill) Terhadap Aplikasi Pupuk Yang Berbeda. Produksi tanaman, 5(5), 774–781.
Armita, D., Wahdaniyah, W., Hafsan, H., & Al Amanah, H. (2022). Diagnosis Visual Masalah Unsur Hara Esensial Pada Berbagai Jenis Tanaman. Teknosains: Media Informasi Sains Dan Teknologi, 16(1), 139–150. https://doi.org/10.24252/teknosains.v16i1.28639
Badan Pusat Statistik Nasional. (2024). Produksi Tanaman Sayuran, 2021-2023. https://www.bps.go.id/id/statistics-table/2/NjEjMg==/produksi-tanaman-sayuran.html
Bhati, N., Shreya, & Sharma, A. K. (2021). Cost-Effective Cellulase Production, Improvement Strategies, And Future Challenges. Journal of Food Process Engineering, 44(2). https://doi.org/10.1111/jfpe.13623
Choudhary, D., Rawat, M., Mashkey, V. K., Sharma, V., & Kundu, P. (2025). Estimation Of Seaweed Extract And Micronutrient Potential To Improve Net Returns By Enhancing Yield Characters In Tomato Using Correlation Analysis. Journal of Applied Biology and Biotechnology, 13(1), 243–249. https://doi.org/10.7324/JABB.2024.199958
Condrain, K. (2017a). The Nutrient Cycle. Seecon Internasional Gmbh. Https://Sswm.Info/Arctic-Wash/Module-2-Environment-Pollution-Levels-Implications/Further-Resources-Environment-And/The-Nutrient-Cycle
Distani. (2022, October 17). Pupuk Hayati Jadi Solusi Efisiensi Penggunaan Pupuk Kimia. Dinas Pertanian Tulang Bawang.
Edufarmers Internasional. (2024). Jumlah Daun Tanaman. Bertani Academy. https://academy.bertani.co/wiki/jumlah-daun-tanaman
Ekowati, C. N., Mirani, M., Handayani, K., & Agustrina, R. (2021). Detection of Nitrogenase Producing Bacteria From the Soil of Liwa Botanical Garden. Jurnal Ilmiah Biologi Eksperimen Dan Keanekaragaman Hayati (J-BEKH), 8(2), 53–58. https://doi.org/10.23960/jbekh.v8i2.204
Faldi, A., Abadi, S., Purwanti, E. W., & Muditha, I. G. N. (2022). Respons Pertumbuhan dan Produktivitas Tomat Terhadap Berbagai Dosis MOL Limbah Buah-Buahan (Response of Tomato Growth and Productivity to Various Doses of MOL from Fruit Waste). 27(1), 103–108. https://doi.org/10.18343/jipi.27.1.103
Febriani, N. (2023). Uji Potensi beberapa Bakteri dalam Melarutkan P, K, dan Fiksasi N Serta Interaksinya terhadap Tanaman Tembakau (Nicotiana tabacum).
Handayanto, E., & Muddarisna, N. A. F. (2017). Pengelolaan Kesuburan Tanah. Universitas Brawijaya Press (UB Press).
Harjanti, R. A., Tohari, & Hidayah Utami, S. N. (2014). Pengaruh Takaran Pupuk Nitrogen dan Silika terhadap Pertumbuhan Awal (Saccharum officinarum L.) pada Inceptisol. Vegetalika, 3(2), 35–44.
Irwan, A. W., & Wicaksono, F. Y. (2017). Perbandingan pengukuran luas daun kedelai dengan metode gravimetri, regresi dan scanner Comparations of soybean’ s leaf area measurement using gravimetry, regression , and scanning. Jurnal Kultivasi, 16(3), 425–429.
Islam, F., & Roy, N. (2018). Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses. BMC Research Notes, 11(1), 1–6. https://doi.org/10.1186/s13104-018-3558-4
Jaya, D. K., Hasibuan, S. Y. K., & Bria, D. (2021). Isolation and Characterization of Potassium-Solubilizing Bacteria from Two Different Rhizospheres and a Cow Manure in IPB University. Jurnal Biologi Tropis, 21(2), 336–342. https://doi.org/10.29303/jbt.v21i2.2559
Kementan RI. (2021, November 6). Mengenal Pupuk Hayati. KEMENTAN, Pusat Perpustakaan Dan Literasi Pertanian.
Ketut, D., Sukmadewi, T., Made, N., Suardani, A., & Candra, I. P. (2022). Isolasi dan Uji Kemampuan bakteri Pelarut Kalium Dari Tanah Sawah Dengan Sistem Irigasi Subak. 10(3), 413–419.
Kurniawan, A., Sari, S. P., Asriani, E., Kurniawan, A., Sambah, A. B., Triswiyana, I., & Prihanto, A. A. (2019). Kapasitas Hidrolisis Bakteri Pendegradasi Selulosa Dari Ekosistem Mangrove. Journal of Tropical Marine Science, 2(2), 76–82. https://doi.org/10.33019/jour.trop.mar.sci.v2i2.1409
Larasati, E. D., Rukmi, I., Kusdiyantini, E., Cinta, R., & Ginting, B. (2018). Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut (Vol. 20, Issue 1).
Lingkungan, T. G. I. (2024). Siklus Nutrisi. StudySmarter. https://www.studysmarter.co.uk/explanations/environmental-science/ecology-research/nutrient-cycling/
Mardaus, Sari, I., & Yusuf, E. Y. (2019). Produksi Tanaman Tomat (Solanum lycopersicum L.) Dengan Pemberian Sp-36 Dan Dolomit Di Tanah Gambut. Jurnal AGROINDAGIRI, 4(2), 25–35. https://doi.org/10.32520/jai.v4i2.1271
Mardyansah, D., & Trimulyono, G. (2021). Isolation, Characterization, and The Potential Test of Phosphate Solubilizing Bacteria from Teak and Sengon Rhizosphere at Limestone Mountains, South Tulungagung Regency: Lentera Bio. 10, 188–198.
Marwantika, A. I. (2020). Pembuatan Pupuk Organik Sebagai Upaya Pengurangan Ketergantungan Petani Terhadap Pupuk Kimia Di Dusun Sidowayah, Desa Candimulyo, Kecamatan Dolopo, Kabupaten Madiun. InEJ: Indonesian Engagement Journal, 1(1), 17–28. https://doi.org/10.21154/inej.v1i1.2044
Murtiyaningsih, H., & Hazmi, M. (2017). Isolasi Dan Uji Aktivitas Enzim Selulase Pada Bakteri Selulolitik Asal Tanah Sampah Isolation And Cellulase Enzyme Activities Assays In Cellulolytic Bacteria Origin From Soil Waste. 135 Agritrop, 15(2), 293–308. http://jurnal.unmuhjember.ac.id/
Nawaz, A., Qamar, Z. U., Marghoob, M. U., Imtiaz, M., Imran, A., & Mubeen, F. (2023). Contribution of potassium solubilizing bacteria in improved potassium assimilation and cytosolic K+/Na+ ratio in rice (Oryza sativa L.) under saline-sodic conditions. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1196024
Nurtika, N., & Gunadi, N. (2009). Respons Tanaman Tomat terhadap Penggunaan Pupuk Majemuk NPK 15-15-15 pada Tanah Latosol pada Musim Kemarau: J. Hort. 19(1), 40–48.
Olaniyan, F. T., Alori, E. T., Adekiya, A. O., Ayorinde, B. B., Daramola, F. Y., Osemwegie, O. O., & Babalola, O. O. (2022). The use of soil microbial potassium solubilizers in potassium nutrient availability in soil and its dynamics. Annals of Microbiology, 72(1). https://doi.org/10.1186/s13213-022-01701-8
Pane RDPP, GInting, E., & Hidayat F. (2022). Mikroba pelarut fosfat dan potensinya dalam meningkatkan pertumbuhan tanaman. Warta Pusat Penelitian Kelapa Sawit, 27(1), 51–59.
Purba, T., Ningsih, H., & Abdus, P. (2021a). Buku Tanah dan Nutrisi Tanaman.
Pusat Statistik, B. (2024). Analisis Produktivitas Jagung Dan Kedelai di Indonesia the Analysis of Maize and Soybean Yield in Indonesia (the Result of Crop-Cutting Survey).
Puspitorini, P., Serdani, A. D., Agroteknologi, P., Balitar, U. I., & Kambing, K. (2025). Pengaruh jenis dan dosis pupuk organik terhadap pertumbuhan dan hasil tanaman tomat (Lycopersicum esculentum Mill.) Varietas servo. 15(1), 1–7.
Rahmah, M. E., Mayasari, U., & Rasyidah. (2024a). Potensi Bakteri Selulolitik Sebagai Biofertilizer Secara In Vitro dari Limbah Padat Pabrik Kelapa Sawit. Biogenerasi, 9(1), 706–715.
Rawat, P., Das, S., Shankhdhar, D., & Shankhdhar, S. C. (2021). Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake. Journal of Soil Science and Plant Nutrition, 21(1), 49–68. https://doi.org/10.1007/s42729-020-00342-7
Rivki, M., Bachtiar, A. M., Informatika, T., Teknik, F., & Indonesia, U. K. (2023). Isolasi Bakteri Pelarut Kalium Rhizosfer Padi di Sragen Sebagai Ketahanan Dan Pertumbuhan Tanaman. Bioeksperimen, 9(112).
Sapalina, F., Noviandi Ginting, E., & Hidayat, F. (2022). Bakteri Penambat Nitrogen Sebagai Agen Biofertilizer. WARTA Pusat Penelitian Kelapa Sawit, 27(1), 41–50. https://doi.org/10.22302/iopri.war.warta.v27i1.80
Simanungkalit, R. D. M., Suriadikarta, D. A., Saraswati, R., Setyorini, D., & Hartatik, W. (2006). Pupuk Organik Dan Pupuk Hayati Organic Fertilizer and Biofertilizer. In Balai Besar Litbang Sumberdaya Lahan Pertanian.
Singh, J. S., Seneviratne, G., & Health, M. C. (2017). Agro-Environmental Sustainability (J. S. Singh & G. Seneviratne, Eds.; Vol. 1). Springer International Publishing. https://doi.org/10.1007/978-3-319-49724-2
Susilo, D. E. H. (2015). Identifikasi Nilai Konstanta Bentuk Daun untuk Pengukuran Luas Daun Metode Panjang Kali Lebar pada Tanaman Hortikultura di Tanah Gambut. Anterior Jurnal, 14(2), 139–146. https://doi.org/10.33084/anterior.v14i2.178
Theresia, V. S., Fandy, H., & Setyono, Y. T. (2019). Pengaruh Pemberian Dosis Pupuk NPK dan Hayati terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium ascalonicum L.) The Effect of NPK fertilizer and Biofertilizer on the Grow... Substitusi pupuk MoP View project Marginal land View project. Jurnal Produksi Tanaman, 7 (2), 2151–2160. https://www.researchgate.net/publication/340116193
Trumble, J. T., Kolodny-Hirsch, D. M., & Ting, I. P. (1993). Plant compensation for arthropod herbivory. Annual Review of Entomology, 38(1), 93–119. https://doi.org/10.1146/annurev.en.38.010193.000521
Wibowo, N. A., Tjahjana, B. E., Heryana, N., & Sakiroh. (2012). Peranan mikroorganisme dalam pengelolaan hara terpadu pada perkebunan kakao. Bunga Rampai: Inovasi Teknologi Bioindustri Kakao, 91–98.
Wicaksono, F. A., Pujawati, E. D., & Payung, D. (2020). Studi Pertumbuhan Nyawai (Ficus variegata blume) dI KHDTK Riam Kiwa Kalimantan SELATAN. Jurnal Sylva Scienteae, 3(3), 509. https://doi.org/10.20527/jss.v3i3.2184
Widowati, T., Simarmata, R., & Nurjanah, L. (2024). Aktivitas Pemacu Pertumbuhan Tanaman dari Bakteri Endofit Bawang Merah (Allium cepa L.). 22(4), 887–893. https://doi.org/10.14710/jil.22.4.887-893.
DOI: https://doi.org/10.14421/biomedich.2026.151.1505-1513
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