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Preventive Effect of Moringa oleifera L. and Solanum lycopersicum Fruit Against Toxicity of Induced Paracetamol Rat Spermatozoa
Abstract
The aim of the research was to determine the preventive effect of Moringa oleifera L. extract and Solanum lycopersicum L. fruit on rat spermatozoa after being induced by toxic doses of paracetamol. This type of experimental research used 30 mice divided into 6 treatments, namely K- (paracetamol induction 500mg/kg BW); K+ (M. oleifera extract 600 mg and S. lycopersicum fruit extract 60 mg/kg BW); P1 (M. oleifera extract 600 mg/kgBW); P2 (S. lycopersicum fruit extract 60 mg/kg BW) and P3 (M. oleifera extract 600 mg and S. lycorpesicum fruit extract 60 mg/kg BW). Test animals at P1, P2, and P3 were previously induced by paracetamol. Treatment was given orally for 15 days, while paracetamol induction was given on day 11. On the 16th day, mice were sacrificed using chloroform and dissected to obtain spermatozoa from the cauda epididymis. Observation variables include motility, viability, and morphology of spermatozoa. Data were analyzed using the Analysis of Variance (ANOVA) test and the Honest Significant Difference further test (P<0.05). The results showed that K+ treatment had a better preventive effect. The percentage of normal motility was 79.33%, the percentage of live spermatozoa was 76.16%, and the percentage of normal morphology was 77.67%. The conclusion of the research is that the combination of M. oleifera extract and S. lycopersicum fruit is preventive in preventing spermatozoa damage caused by the effects of excessive use of paracetamol.
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DOI: https://doi.org/10.14421/biomedich.2026.152.2089-2096
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