Ascorbic Acid and Carotenoid Contents in Tomato Callus: Effects of Explant Source and Subculture

Imam Purwanto, Yulita Nurchayati, Erma Prihastanti

Abstract


The production of natural compounds in tomato can be achieved through callus culture by selecting the most suitable explant. The objectives of this study were to examine the effects of explant source and culture maintenance on the growth, ascorbic acid content, and carotenoid content of tomato callus. Callus induction was carried out on MS medium supplemented with 2,4-D. The resulting calli were divided into two groups: one subcultured into fresh medium and the other maintained without subculture. The study design used a completely randomized design of two factors, they were explants source (cotyledons, hypocotyls and root sprouts) and subculture treatment. The observed parameters were growth of callus, carotenoid and ascorbic acid content analized by spectrophotometer. The results showed that the highest callus weight (0.29 g) was obtained from hypocotyl explants. Subculture treatment significantly affected ascorbic acid content but did not influence carotenoid content. Callus from cotyledon explants produced the highest ascorbic acid content (15.2 g/100 g). Subculture treatment increased callus fresh weight, carotenoid content, and ascorbic acid content compared to the non-subculture treatment. In conclusion, hypocotyls were the best explant source for increasing biomass, while cotyledons were the most effective for enhancing ascorbic acid production.


Keywords


Callus culture; Explants; Secondary Metabolites; Subculture.

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Al-Oqab, M. A., Zaid, S., Al-Ammouri, Y., and Alawdi, S. H. (2023). Effect of nutrient media enhanced with plant growth regulators on genetic stability in sub-cultures of Digitalis purpurea callus. Journal of Applied Biology & Biotechnology, 11(2), 1–9. DOI: https://doi.org/10.7324/JABB.2023.110227.

Ariani R, Anggraito YU, Rahayu ES. (2016). Respons Pembentukan Kalus Koro Benguk (Mucuna Pruriens L.) pada Berbagai Konsentrasi 2,4-D dan BAP. Indonesian Journal of Mathematics and Natural Science, 39(1), 20?28. DOI: https://doi.org/10.15294/ijmns.v39i1.7695.

Cardoso, J. C., Oliveira, M. E. B. D., dan Cardoso, F. D. C. (2019). Advances and Challenges on the in Vitro Production of Secondary Metabolites from Medicinal Plants. Horticultura Brasileira, 37(2), 124– 132. DOI: https://doi.org/10.1590/S0102-053620190201.

Felfoldi, Z., Ranga, F., Roman, I.A., Sestras, A.F., Vodnar, D.C., Prohens, J., and Sestras, R.E. (2022). Analysis of Physico-Chemical and Organoleptic Fruit Parameters Relevant for Tomato Quality. Agronomy, 12, 1232. DOI: 10.3390/agronomy12051232

Fitriana, N., Semiarti, E., & Rumiyati. (2017). Respon Pertumbuhan dan Kandungan Karotenoid Kalus dari Kecambah Wortel (Daucus carota L.) dengan Jenis Eksplan yang Berbeda. Skripsi S1 Biologi, Universitas Gadjah Mada.

Handayani, S. U. dan T. D. Kurnia. (2018). Seleksi 25 Genotif F1 Tomat (Lycopersicon esculentum) Berdasarkan Potensi Hasil dan Karakter Morfologi Buah. Fakultas Pertanian dan Bisnis. Universitas Kristen Satya Wacana.

Huh, Y. S., Lee, J. K., and Nam, S. Y. (2017). Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims). Journal of Plant Biotech. 44(3), 335-342. DOI:https://doi.org/10.5010/JPB.2017.44.3.335.

Hussein, S., Halmi, M. I. E. and Kiong, A. L. P. 2016. Modelling the growth kinetics of callus cultures from the seedling of Jatropha curcas L. according to the modified Gompertz model. Journal of Biochemistry, Microbiology and Biotechnology, 4, 20-23. DOI: https://doi.org/10.54987/jobimb.v4i1.284

Indriani, R., Prihastanti, E., Budihastuti, R., & Nurchayati, Y. (2020). Effect of Subculture Frequency Toward Growth and Carotenoid Content from Tomato (Lycopersicon esculentum Mill.) Callus. Jurnal Biodjati, 5(2), 303-315. DOI: https://doi.org/10.15575/biodjati.v5i2.5840

Iskandar, N.N. and Iriawati. (2016). Vinblastine and Vincristine Production on Madagascar Periwinkle (Catharanthus roseus (L.) G. Don) Callus Culture Treated with Polethylene Glycol. Makara Journal of Science, 20/1, 7-16.

DOI: https://doi.org/10.7454/mss.v20i1.5656

Jamil, S.Z.M.R., · E.R. Rohani1 · Syarul Nataqain Baharum, and N. M. Noor. (2018). Metabolite profiles of callus and cell suspension cultures of mangosteen. Biotech, 8, 322. DOI: https://doi.org/10.1007/s13205-018-1336-6

Julianti, R.F., Nurchayati, Y., & Setiari, N. (2021). Pengaruh Konsentrasi Sukrosa dalam Medium MS terhadap Kandungan Flavonoid Kalus Tomat (Solanum lycopersicum syn. Lycopersicum esculentum). Metamorfosa: Journal of Biological Sciences, 8(1), 141–149. https://doi.org/10.24843/metamorfosa.2020.v08.i01.p015

Nakasha, J. J., Sinniah, U. R., Kemat, N., & Mallappa, K. S. (2016). Induction, subculture cycle, and regeneration of callus in safed musli (Chlorophytum borivilianum) using different types of phytohormones. Pharmacognosy Magazine, 12 (Suppl 4), S460-S464. DOI:10.4103/0973-1296.191457.

Nurchayati, Y., Prihastanti, E., & Budihastuti, R. (2023). In-vitro Callus Development and the Bioactive Compounds of Tomato (Lycopersicon esculentum Mill.). Al-Kauniyah: Jurnal Biologi, 16(1), 89–97. https://doi.org/10.15408/kauniyah.v16i1.21565

Nurchayati, Y., Santosa, L. H. Nugroho, A. Indrianto. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel L. Biosaintifika, 8(2), 135-140. DOI: 10.15294/biosaintifika.v8i2.5177.

Oleszkiewicz, T., Klimek-Chodacka, M., Milewska-Hendel, A., Zubko, M., Stróz, D., Kurczy?ska, E., Boba, A., & Baranski, R. (2018). Unique chromoplast organisation and carotenoid gene expression in carotenoid-rich carrot callus. Planta, 248(6), 1455–1471. https://doi.org/10.1007/s00425-018-2988-5

Perangin Y. Yayuk, P. Yenni, A. Murni, S.R. Nurhayati. (2019). Pemanfaatan kandungan metabolit sekunder yang dihasilkan tanaman pada cekaman biotik. Jurnal Agriland. 7(1), 39-47. DOI: https://doi.org/10.30743/agr.v7i1.3471.

Pillai, S. K., and E.A. Siril. (2019). Enhanced Production of Berberine Through Callus Culture of Tinospora cordifolia (Willd.) Miers ex Hook F. and Thoms. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 1-9. DOI: https://doi.org/10.1007/s40011-019-01106-9.

Rasud, Y., and Bustaman. (2020). Induksi Kalus secara In Vitro dari Daun Cengkeh (Syizigium aromaticum L.) dalam Media dengan Berbagai Konsentrasi Auksin. Jurnal Ilmu Pertanian Indonesia, 25(1), 67-72. DOI: https://doi.org/10.18343/jipi.25.1.67.

Rodinah R, Hardarani N, and Ariani, H.D. (2018). Modifikasi Media Dan Periode Subkultur pada Kultur Jaringan Pisang Talas (Musa paradisiaca VAR. SAPIENTUM L.). Jurnal Hexagro, 2(2), 1–6. DOI: https://doi.org/10.36423/hexagro.v2i2.129

Schaub, P., Rodriguez-Franco, M., Cazzonelli, C.I., Álvarez, D.,Wüst, F., and Welsch, R. (2018). Establishment of an Arabidopsis callus system to study the interrelations of biosynthesis, degradation and accumulation of carotenoids. PLoS ONE , 13. DOI: https://doi.org/10.1371/journal.pone.0192158.

Ulva, M., Y. Nurchayati, N. Setiari, and E. Prihastanti. (2019). Pertumbuhan Kalus Tomat (Lycopersicon esculentum Mill.) Varietas Permata F1 dari Jenis Eksplan dan Konsentrasi Sukrosa yang Berbeda secara In Vitro. Life Science, 8 (2), 160-169. DOI: https://doi.org/10.15294/lifesci.v8i2.37103.

Wang, G., Ren, Y., Bai, X., Su, Y., and Han, J. 2022. Contributions of Beneficial Microorganisms in Soil Remediation and Quality Improvement of Medicinal Plants. Plants, 11(23), 3200. DOI: https://doi.org/10.3390/plants11233200.

Wei, Y., Wang, X., Shao, X., Xu, F., and Wang, H. (2019). Sucrose treatment of mung bean seeds results in increased vitamin C, total phenolics, and antioxidant activity in mung bean sprouts. Food Science & Nutrition, 7(12), 4037–4044. DOI: https://doi.org/10.1002/fsn3.1269

Wu, K., Liu, Y., Xu, Yuven., Yu, Z., Cao, Q., Gong, H, Yang, Y., Ye, J., and Jia, X. (2024). Unveiling the molecular mechanisms of browning in Camellia hainanica callus through transcriptomic and metabolomic analysis. International Journal of Molecular Sciences, 25(20), 11021. DOI: https://doi.org/10.3390/ijms252011021.




DOI: https://doi.org/10.14421/biomedich.2026.151.525-533

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Copyright (c) 2026 Imam Purwanto, Yulita Nurchayati, Erma Prihastanti



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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