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Physicochemical, Antimicrobial, Lethality and In Vitro Antioxidant Profiles of Johnu Tisane: A Coffee (Coffea Arabica) Leaf Decoction Formula


 
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1. Title Title of document Physicochemical, Antimicrobial, Lethality and In Vitro Antioxidant Profiles of Johnu Tisane: A Coffee (Coffea Arabica) Leaf Decoction Formula
 
2. Creator Author's name, affiliation, country Anthony Cemaluk Chinedum Egbuonu; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Prince Ogochukwu Alaebo; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Udumma Nsofor Onuoha; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Chinomso Juliet Njoku; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Chiemeziem Adanma Obike; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Susan Ogechi Nlemadim; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Blessing Ifeoma Chukwu; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Sopuruchi Mary-Augusta Iwejuo; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Onyedikachi Glory Amaechi; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Victor Ifechukwu Obiefuna; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Bessing Nmesomachi Onuoha; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country DivineTreasure Nzubechukwu Okoli; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Precious Chinonso Nwokeoma; Michael Okpara University of Agriculture Umudike; Nigeria
 
2. Creator Author's name, affiliation, country Chukwubuikem Eze; Michael Okpara University of Agriculture Umudike; Nigeria
 
3. Subject Discipline(s)
 
3. Subject Keyword(s) In-vivo anti-oxidative outcomes and roles; Johnu tisane; Requisite physicochemical mix; Requisite anti-oxidant capacity; Rich phyto-constituents-related diverse pharmacologic activities
 
4. Description Abstract Coffee leaves-based tea has relevance in ethno-medications due to its rich phyto-constituents-related diverse pharmacologic activities. Jonhu tisane, a typical Coffea arabica leaf decoction has no supporting scientific basis amidst reported location, processing and specie-related variations. This investigated physicochemical, antimicrobial, lethality and in-vitro antioxidant profiles of Johnu tisane by acceptable methods. Results recorded moisture (92.26 %), ash (0.65 %), unsaponified matter (1.46 mg/100 g), free fatty acid (0.56 mgKOH/g), acid value (1.12 mgKOH/g), potential hydrogen (6.85), lethal concentration (1000 ppm) and concentration-dependent antimicrobial activity. Anti-oxidation results revealed total antioxidant capacity, ferric reducing antioxidant power, nitric oxide, hydrogen peroxide and 2, 2-diphynyl-1-picrylhydrazyl scavenging activities increased concentration-dependently compared to standard. Thus, Johnu tisane demonstrated low minerals and keeping quality; requisite physicochemical mix for consumption and bioactivity; high safety margin; antimicrobial potency; and requisite anti-oxidation capacity for in-vivo antioxidant role. These provided scientific support for its ethno-medicinal uses. They underscored the need to elucidate its pharmacologically active compounds; mechanistic roles in animal models; and the impact of ash to moisture mix variation on potential hydrogen, microbial and antimicrobial activities in relation to shelf life, bioactivity and in vivo anti-oxidative roles for novel insights on preserving sample quality, safety, bioactivity and in-vivo anti-oxidative outcomes.
 
5. Publisher Organizing agency, location Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
 
6. Contributor Sponsor(s)
 
7. Date (YYYY-MM-DD) 2024-05-15
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier https://sciencebiology.org/index.php/BIOMEDICH/article/view/462
 
10. Identifier Digital Object Identifier (DOI) https://doi.org/10.14421/biomedich.2024.131.99-107
 
11. Source Title; vol., no. (year) Biology, Medicine, & Natural Product Chemistry; Vol 13, No 1 (2024)
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2024 Anthony Cemaluk Chinedum Egbuonu, Prince Ogochukwu Alaebo, Udumma Nsofor Onuoha, Chinomso Juliet Njoku, Chiemeziem Adanma Obike, Susan Ogechi Nlemadim, Blessing Ifeoma Chukwu, Sopuruchi Mary-Augusta Iwejuo, Onyedikachi Glory Amaechi, Victor Ifechukwu Obiefuna, Bessing Nmesomachi Onuoha, DivineTreasure Nzubechukwu Okoli, Precious Chinonso Nwokeoma, Chukwubuikem Eze