A Simple Method for Clearing and Staining Specimens for The Demonstration of Animal Skeleton

Muhammad Ja’far Luthfi, Nyoman Puniawati Soesilo


Teaching skeletal system would be more attractive using real/preserved specimen compared to those using only book. The aim of the research was to develop clearing and staining method of animal skeleton specimen using Alizarin Red S-Alcian Blue as teaching aid tool. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. The spesimen will increase effectiveness in elementary school science teaching and learning.


skeleton; Alizarin Red S; Alcian Blue; elementary school; teaching aid

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DOI: https://doi.org/10.14421/biomedich.2019.81.17-21


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Copyright (c) 2019 Muhammad Ja’far Luthfi, Nyoman Puniawati Soesilo

Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

This work is licensed under a CC BY-NC