The Antivirulence Mechanisms of Phytate Against Pathogenic Bacteria in Skin Infections

Nabilatul Zhofiroh, Rian Ka Praja, Elsa Trinovita, Ysrafil Ysrafil, Ranintha BR Surbakti

Abstract


Skin infections caused by the bacteria Staphylococcus aureus, Streptococcus pyogenes, and Propionibacterium acnes are often a common health problem. One treatment is antibiotics, but the cases of antibiotic resistance are increasing. Thus, new treatment alternatives are needed. This study aimed to analyze the molecular mechanism of phytate antivirulence against pathogenic bacteria of skin infection. This study used a bioinformatics approach involving analysis of phytate interactions with bacterial virulent proteins via STITCH, functional classification of proteins with VICMpred, and prediction of virulence properties using VirulentPred. B-cell and MHC epitopes were analyzed using IEDB, while protein subcellular location was determined through PSORTb. The results showed that phytate interacted specifically with virulent proteins in all three bacteria, most of which functioned in cellular and metabolic processes. These virulent proteins also have immunologically relevant epitopes. Subcellular location analysis showed that phytate protein targets were dispersed in the cytoplasmic membrane and cytoplasm. These findings indicated that phytate has a significant antivirulence mechanism by targeting virulent proteins of skin pathogenic bacteria, thus potentially becoming a therapeutic agent to treat skin infections while reducing antibiotic resistance.

Keywords


Phytate; Antivirulence; Staphylococcus aureus; Streptococcus pyogenes; Propionibacterium acnes

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References


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DOI: https://doi.org/10.14421/biomedich.2025.142.731-738

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Copyright (c) 2025 Nabilatul Zhofiroh, Rian Ka Praja, Elsa Trinovita, Ysrafil Ysrafil, Ranintha BR Surbakti



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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