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Subacute Toxicity Study of Leaf Extract of Saccharum officinarum
Abstract
Saccharum officinarum (Family-Poaceae) is used traditionally to treat malaria and fever, among others. Evaluation of subacute administration ethanol leaf extract of Saccharum officinarum for possible effect on hematological indices, liver and kidney functions, and lipid profile of rats was carried out. The leaf extract (170, 340, 510 mg/kg body weight) was orally administered to male Wistar rats daily for 30 days, and the rats were sacrificed under light diethyl ether anesthesia after the administration. Subacute administration of S. officinarum leaf extract resulted in an insignificant increase in the body weights of rats without any significant (p>0.05) effect on the weights of liver and kidney when compared to control. The leaf extract treatment did not affect WBC, lymphocytes, monocytes, eosinophil, and basophil percentages. However, it caused significant (p<0.05) decreases in RBC and platelet counts, hemoglobin concentration, and PCV percentage, especially at the middle dose (374 mg/kg), and also prolonged bleeding and clotting time significantly (p<0.05) when compared to control. The leaf extracts non-dose-dependently caused insignificant (p>0.05) decreases in total protein, albumin, and ALT levels. ALP was significantly (p<0.05) decreased at the highest dose (510 mg/kg). However, AST, total and conjugated bilirubin levels were significantly (p<0.01-0.001) decreased only at higher doses (340 and 510 mg/kg) of the extract. The leaf extract did not cause any significant (p>0.05) effect on urea, creatinine, potassium, and sodium as well as total cholesterol, triglyceride, VLDL, and LDL levels of rats, but the highest dose (510 mg/kg) significantly (p<0.05) increased Cl level and reduced HDL level of rats when compared to control. The leaf extract exerts mild to moderate effects on the histology of the livers and kidneys of rats. Chronic study is advocated to investigate the effect of prolonged administration of rats’ extract organs and systems.
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Abolaji, AO., Adebayo, AH. and Odesanmi, OS. (2007). Effect of ethanolic extract of Parinari polyandra (Rosaceae) on serum lipid profile and some electrolytes in pregnant rabbits. Research Journal of Medicinal Plants 1: 121- 127.
Akwetey GA, Achel DG.(2010). Ethnopharmacological use of herbal remedies for the treatment of malaria in the Dangme West District of Ghana. J Ethnopharmacol 129 (3): 367-376.
Ali, B.H., Ben, T.H. and Basheer, A.A. (2001). Sex related differences in the susceptibility of rat to gentamicin nephrontoxicity: influence of gonad-ectomy and hormonal replacement therapy. Indian Journal of Pharmacology.33: 369-373.
Ashafa, A. O. T., Yakubu M. T., Grierson D. S. and Afolayan,A. J. (2009).Effects of aqueous extract from the leaves of Chrysocoma ciliata L. on some biochemical parameters of Wistar rats. African Journal of Biotechnology 8 (8): 1425-1430.
Bashir, L., Shittu, O. K., Busari, M. B., Sani, S., and Aisha, M. I. (2015). Safety evaluation of giant African land snails (Archachatina maginata) haemolymph on hematological and biochemical parameters of albino rats. Journal of Advanced Medicine and Pharmaceutical Science, 3(3), 122-30.
Berinyuy, E. B., Lawal, B., Olalekan, A. A., Olalekan, I. A., Yusuf, A. A., Sakpe, S., and Ossai, P. C. (2015). Hematological status and organs/body-weight parameters in Wister rats during chronic administration of Cassia occidentalis.International Blood Research and Review, 4(3), 1-7.
Coutinho ID,Baker JM, Ward JL, Beale MH, Creste S,Cavalheiro AJ. (2016). Metabolite profiling of sugarcane genotypes and identification of flavonoid glycosides and phenolic acids J. Agric. Food Chem.2016, 64, 21, 4198–4206.
Dufour, D.R., Lott, J.A., Nolte, F.S., Gretch, D.R., Koff, R.S. and Seeff, L.B. (2000). Diagnosis and monitoring of hepatic injury: I. Performance characteristics of laboratory tests. Clinical Chemotheraphy. 46:2027– 2049.
Edem UA, Okokon JE, Bassey AL, Okokon PJ. (2022).Antioxidative stress and hepatoprotective activities of leaf extract and fractions of Sacharum officinarum in Plasmodium berghei infected mice. Journal of Current Biomedical Research.2(4):317-337.
Edem UA, Udobang JA, Okokon JA.(2023a). Antiinflammatory and antipyretic activities of ethanol leaf extract of Saccharum officinarum in mice. Journal of Medical and Pharmaceutical Sciences.10(8):29-36.
Edem UA, Okokon JE, Bassey AL, Iyanyi UL. (2023b). Antiulcer activity of leaf extract of Saccharum officinarum. Nigerian Journal of Pharmaceutical and Applied Sciences Research 12(2):22-27.
Evans, G.O. and Whitehorn, L.C. (1995). Effects of pyridoxal 5’-phosphate on plasma alanine aminotransferase determinations in toxicological studies. Toxicology Letter. 80: 34-37.
Farah AO, Nooraain H, Noriham A, Azizah AH, Nurul HR. (2013). Acute and oral subacute toxicity study of ethanolic extract of Cosmos caudatus leaf in Sprague Dawley Rats. International Journal of Biosciences, Biochemistry and Bioinformatics. 3(4): 301-305.
Flaoyen, A., Hove, K. and Wilkins, A.L. (2001). Tolerance to nephrotoxic component of Narthecium ossifragum in sheep: The effects of repeated oral doses of plant extracts. Vetinary Research and Communication disease. 25: 127-136.
Hall, R.L. (2001). Principles of clinical pathology for toxicology studies. In Principles and methods of toxicology 4th Ed, ed A Wallace Hayes Taylor and Francis, Philadelphia.
Hartwell, J.L. 1967–1971. Plants used against cancer. A survey. Lloydia 30–34.
Jackson J (1996). Diet and Health: Implication for reducing chronic disease risks. National Research Council. National Academic Press, Washington DC.
Kaplan A, Szabo LVL. and Opheim KE. (1979). Clinical Chemistry: Interpretation and Techniques: Lea & Febiger Philadelphia; 1979.
Lum, G. (1995). Low activities of aspartate and alanine aminotransferases. Their significance in alcoholic liver disease. Laboratory Medicine. 26: 273–276.
Mayes PA (1996). Lipid transport and storage. In: Harper’s biochemistry. Murray RK, Granner DK, Mayes PA, Rodwell VW (Eds), 24th ed. Prentice Hall International, Inc., USA, pp. 254- 255.
Mayne, P.D. (1994). The kidneys and renal calculi. In: Clinical chemistry in diagnosis and treatment. 6th ed. London: Edward Arnold Publications, pp.2-24.
Naganna B. (1989). Plasma proteins. In: Tawlar GP, Srivastava LM, Moudgil KD (Eds.). Textbook of Biochemistry and Human Biology. 2nd edn. India: Prentice-Hall of India Private Ltd. p. 172.
Obidike IC, AmoduB, EmejeMO. (2015). Antimalarial properties of SAABMAL®: an ethnomedicinal polyherbal formulation for the treatment of uncomplicated malaria infection in the tropics.Indian J Med Res. 141(2): 221–227.
Ojewunmi O, Oshodi T, Ogundele O, Micah C and Adenekan S.(2013). Evaluation of the anti-diabetic and antioxidant activities of aqueous extracts of Morinda lucida and Saccharum officinarum leaves inalloxan-induced diabetic rats. Intl J. Biochemistry Res Review, 3(3): 266-277.
Okokon JE, Mobley R, Edem UA, Bassey AL, Fadayomi I, Horrocks P, Drijfhout F, Li WW. (2022). In vitro and in vivo antimalarial activities and chemical profiling of sugarcane leaves. Science Reports 41598 Article No:14391 doi. org/ 10. 1038/ s41598 -022-14391-8.
Okokon JE, Davies K, Edem UA, Bassey AL, Udobang JA.(2021). Analgesic activity of ethanol leaf extract of Saccharum officinarum.Trop J Nat Prod Res. 5(6):1142-1145.
Okokon JE, Udoh AE, Nyong EE, Eno L, Udo NM.(2019). Psychopharmacological studies on leaf extract of Saccharum officinarum. Trop J Nat Prod Res. 3(2):26-30.
Palaksha MN, Ravishankar K and Girijasastry V. (2013). Phytochemical screening and evaluation of in-vitro antibacterial and anthelmintic activities of Saccharum officinarum leaf extracts. World J Pharmacy and Pharmaceutical Sci, 2(6):5761-5768.
Palaksha, M. N., Ravishankar, K., GirijaSastry, V. (2015). Biological evaluation of in vivo diuretic, and antiurolithiatic activities of ethanolic leaf extract of Saccharum officinarum. Indo Amer J Pharm Res. 5(06): 2232-2238.
Panagiotakos B, Pitsavos C, Skoumas J, Chrysohoou C, Toutouza M, Stefanadis CI, Toutouzas PK (2003). Importance of LDL/HDL ratio as a predicator for coronary heart disease events in patients with heterozygous familial hypercholesterolemia: A 15-year follow-up (1987-2002). Journal of Current Medical Research and Opinion., 19: 89-94.
Phillip DM (1995). Plasma enzyme in diagnosis. In: Clinical chemistry in diagnosis and Treatment, 6th ed. Arnold Publishers, London.p.303- 307.
Ping KY, Darah I, Chen Y, Sreeramanan S and Sasidharan S. (2013). Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats. Biomedical Research International. 2: 1-14.
Rej, R. (1977). Review: the role of coenzymes in clinical enzymology. Annual Clinical Lab Science.7: 455–468.
Saori, N., Kimio, S., Makoto, M., Tadashi, H. and Yoshihide, O. (2003). Hepatic Alanine-glyoxylate Aminotransferase Activity and Oxalate Metabolism in Vitamin B6 Deficient Rats. The Journal of Urology. 2003; 169:683-686.
Shin, M.O., Yoon, S. and Moon, J.O. (2010). The proanthocyanidins inhibit dimethylnitrosamine-induced liver damage in rats. Archieve Pharmacology Research. 33:167-173.
Shittu, O. K., Lawal, B., Abubakar, N. A. Berinyuy, B. E., Busari, M. B., & Ibrahim, A. O. (2015b). Toxicological implications of methanol extract from Nigerian bee propolis on some selected rat tissues. Journal of Pharmacology and Biomedical Science, 5(7), 524-31.
Shittu, O. K., Lawal, B., Alozieuwa, B. U., Haruna, G. M., Abubakar, A. N., &Berinyuy, E. B. (2015a). Alteration in biochemical indices following chronic administration of methanolic extract of Nigeria bee propolis in Wistar rats. Asian Pacific Journal of Tropical Disease, 5(8), 654-657.
Singh A, Lal UR,Mukhtar HM, Singh PS, Shah G, Dhawan RK.(2015). Phytochemical profile of sugarcane and its potential health aspects.Pharmacogn Rev. 9(17): 45–54.
Sun J, He X, Zhao M, Li L, Li C, Dong Y. (2014). Antioxidant and Nitrite-Scavenging Capacities of Phenolic Compounds from Sugarcane (Saccharum officinarum L.) Tops Molecules 2014, 19: 13147-13160.
Tepongning, R.N., Mbah, J.N., Avoulou, F.L., Jerme, M.M., Ndanga, E-K, K. and Fekam, F.B. (2018). Hydroethanolic extracts of Erigeron floribundus and Azadirachta indica reduced Plasmodium berghei parasitaemia in Balb/c Mice. Evidence-based Complementary and Alternative Medicine. 2018: 5156710. doi: 10.1155/2018/5156710.
Tietz, W W. 1990. Clinical Guide to Laboratory tests. 2nd edn. Sanders Company. Philadelphia, PA. pp. 554-556.
Waner, T. and Nyska, A. (1991). The toxicological significance of decreased activities of blood alanine and aspartate aminotransferase. Vetenary Research Committee. 15: 73-78.
Yakuba MT., Bilbis LS., Lawal M. and Akanji MA. (2003). Evaluation of selected parameters of rat liver and kidney function following repeated administration of yohimbine. Biochemistries. 15(2): 50-6.
Yakubu MT., Akanji MA., and Oladiji AT (2008). Alterations in serum lipid profile of male rats by oral administration of aqueous extract of Fadogia argrestis stem. Research of Journal Medicinal Plant. 2: 66-73.
Yakubu, M. T., Akanji, M. A., and Oladiji, A. T. (2005). Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats. Asian Journal of Andrology,7(4), 399-404.
DOI: https://doi.org/10.14421/biomedich.2025.141.91-100
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