Investigating the Insecticidal Properties of Alangium salviifolium Root Extracts on Culex quinquefasciatus Mosquitoe

Alex Yagoo, M.C. John Milton, Jelin Vilvest


This study evaluated the effectiveness of successive hexane, chloroform, and methanol extracts of Alangium salviifolium roots in combating mosquito-borne diseases caused by Culex quinquefasciatus mosquitoe in India. The hexane extract showed the highest efficacy with LC50 values of 105.0 ppm (larvicidal activity), 108.0 ppm (pupicidal activity), and 65.6 ppm (ovicidal activity). The chloroform extract had LC50 values of 156.8 ppm (larvicidal), 376.0 ppm (pupicidal), and 20.0 ppm (ovicidal), while the methanol extract had LC50 values of 235.7 ppm (larvicidal), 441.8 ppm (pupicidal), and 30.4 ppm (ovicidal). The hexane extract of A. salviifolium roots shows potential as a mosquito control agent, specifically against Cx. quinquefasciatus mosquitoes. Further studies are needed to assess the safety and effectiveness of this extract. This research highlights the potential of plant extracts as alternative mosquito control agents for mitigating mosquito-borne diseases in India.


Alangium salviifolium roots; Culex quinquefasciatus; plant extract; toxicity

Full Text:



Abbot WS (1925) A method for computing the effectiveness of an insecticide. Ecol.Entomol.18, 265-267.

Alvarado-Esquivel C, González-López EM, & Cerrillo-Soto SM (2021) Vector-borne diseases in Mexico: A systematic review. Acta Tropica, 218, 105927. 10.1016/j.actatropica.2021.105927

Ali SI, Venkatesalu V. (2020). Evaluation of the larvicidal potential of root and leaf extracts of Saussurea costus (Falc.) Lipsch. against three mosquito vectors: Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. Rev Soc Bras Med Trop. 10.1590/0037-8682-0018-2019

Ansari MA, Ahmad A, & Khan MA (2017) Bioactivity of Alangium salvifolium against red flour beetle, Tribolium castaneum. Biocatalysis and Agricultural Biotechnology, 9, 162-166.

Bhatt S, Gething PW, Brady OJ (2013) The global distribution and burden of dengue. Nature. 496(7446):504-507. 10.1038/nature12060

Baskar K, Ignacimuthu S, Paulraj GM, Alwin R. (2011) Insecticidal and nematicidal properties of some Indian medicinal plants. Journal of Medicinal Plants Research, 5(22), 5375-5379. 10.5897/JMPR11.977

Das NG, & Dhiman S (2015) Mosquito repellent action of neem (Azadirachta indica) oil. Journal of Vector Borne Diseases, 52(1), 59-66. 10.4103/0972-9062.163010

Dhanasekaran S, Suresh J, Ramar M, Karthi S, Shivakumar MS (2018) Oviposition deterrent and ovicidal activities of medicinal plant extracts against Aedes aegypti L. and Culex quinquefasciatus Say (Diptera: Culicidae). Journal of Parasitic Diseases, 42(1), 22-30. 10.1007/s12639-017-0917-8

Finney DJ (1971) Probit Analysis. Cambridge University Press, London, pp.68-78.

Govindarajan M, Rajeswary M, & Sivakumar R (2015) Mosquito larvicidal properties of Feronia limonia, Cleistanthus collinus and Pteridium aquilinum plant extracts against Culex quinquefasciatus. Journal of King Saud University-Science, 27(4), 340-346. 10.1016/j.jksus.2014.10.003

Isman MB (2006) Botanical insecticides: for richer, for poorer. Pest Management Science, 62(11), 1107-1112. 10.1002/ps.1292

Kavitha V, Murugan K, Senthil-Nathan S, Shivakumar MS. (2018) Repellent and insecticidal activity of Alangium salvifolium (L.f.) Wang against the stored grain pest Sitophilus oryzae (L.). Journal of Pharmacognosy and Phytochemistry, 7(2), 1741-1744.

Marimuthu S, Rahuman AA, Santhoshkumar T, Jayaseelan C, & Kirthi AV (2019) Larvicidal activity of Andrographis paniculata against Aedes aegypti (Linnaeus) and Culex quinquefasciatus (Say). Journal of King Saud University-Science, 31(4), 1004-1009.

Mondal, r. P., ghosh, a., burman, s., & chandra, g. (2022). Efficacy of ethyl acetate extract of Alangium salviifolium fruit pericarp against Culex quinquefasciatus larvae. Notulae Scientia Biologicae, 14(2), 11251.

Ministry of Health and Family Welfare (2020) National health profile 2019.

National Vector Borne Disease Control Programme (2020) Annual report 2019-20.

Reegan AD, Saravana Kumar P, Asharaja AC, Devi C, Jameela, S, Balakrishna K, Ignacimuthu S (2021) Larvicidal and ovicidal activities of phenyl acetic acid isolated from Streptomyces collinus against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae), Exp Parasitol, 226-227.

Saxena V, Pandey S, Kerketta P, Pandey A (2013) Larvicidal potential of some weeds against mosquito species Aedes aegypti and Anopheles stephensi. Journal of Medicinal Plants Research, 7(6), 252-257. 10.5897/JMPR12.817

Sharma P, Mohan L, Srivastava CN, Singh SK, Tripathi YB (2015) Evaluation of larvicidal and pupicidal potential of botanical extracts against medically important mosquitoes. Journal of Mosquito Research, 5(12), 1-8. 10.5376/jmr.2015.05.0012

Shukla R, Mishra A, Singh P, & Singh RK (2019) Larvicidal potential of Alangium salvifolium (L.f.) Wangerin (Cornaceae) against Aedes aegypti (Diptera: Culicidae). Journal of Parasitic Diseases, 43(4), 719-723.

Tack W, Madder M, Baeten L, De Frenne P, & Gruwez R (2021) Vector-borne diseases and their relevance for public health in the European Union. Heliyon, 7(2), e06260. 10.1016/j.heliyon.2021.e06260

Tabanca N, Bernier UR, Tsikolia M, Becnel JJ, Sampson BJ, Agramonte NM, Zhang J, Wedge DE (2017) Efficacy of plant-derived and synthetic compounds on clothing as repellents against Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae). Journal of Economic Entomology, 110(2), 576-581. 10.1093/jee/tow312

World Health Organization (2021) Mosquito-borne diseases.

World Health Organization (2020) World malaria report 2020.

World Health Organization (2020) Dengue and severe dengue.

WHO (2005) Guidelines for laboratory and field testing of mosquito larvicides. WHO Geneva. 13, WHO_CDS_WHOPES_GCDPP_2005.

Yagoo, A., Milton, M.C.J., Vilvest, J., Johnson, I., Balakrishna, K., 2023. Mosquito larvicidal, pupicidal and ovidical effects of the different extracts of the leaves of Peltophorum pterocarpum against Aedes aegypti and Culex quinquefasciatus. Futur J Pharm Sci 9, 32.



  • There are currently no refbacks.

Copyright (c) 2023 Alex Yagoo, M.C. John Milton, Jelin Vilvest

Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

This work is licensed under a CC BY-NC