Chemical Composition and Evaluation of Anti-tyrosinase and Anti-Oxidative Effects of Topical Cream Formulation from Acacia sieberiana, Vitellaria paradoxa and Beeswax

Alfred Ngenge Tamfu, Alain Yaya Koudoro, Selcuk Kucukaydin, Theophile Olaye, Pascal Dossa Cokou Agbangnan, Dominique Codjo Koko Sohounhloue, Dominique Codjo Koko Sohounhloue, Felicien Avlessi, Felicien Avlessi


Skin diseases can get natural therapies from medicinal plant-based products. In this study, a topical cream was formulated from ethanol extract of Acacia sieberiana, beeswax and Vitellaria paradoxa (shea) butter. GC-MS characterization with co-injection of the topical cream revealed Stearic acid (31.43%), Palmitic acid (23.15%), Oleic acid (21.44%) and Linoleic acid (16.20%) as the major components. Seven phenolic conpounds were identified and quantified by HPLC- DAD and Ferulic acid (12.81±0.26 mg/g) was the most abundant. The cream showed good antioxidant properties evaluated through ?-Carotene-linoleic acid assay, DPPH radical scavenging, ABTS•+ assay, CUPRAC assay, and metal chelating assay. The cream had higher activity in the DPPH assay (IC50 = 32.10±0.84 µg/mL), ABTS•+ assay (IC50 = 22.49±0.62 µg/mL) and CUPRAC assay (IC50 = 49.27±0.79 µg/mL) than ?-Tocopherol. The antioxidant effects are an indication that the cream can reduce oxidative stress on the skin including aging, carcinogenesis and inflammation. At 100 µg/mL, the topical cream showed tyrosinase inhibition of 48.23±0.87% regarded as relatively good compared to the standard tyrosinase inhibitor kojic acid, which showed 79.50±0.32% inhibition at the same concentration. The cosmetic cream was able to inhibit the melanin production rate-limiting enzyme, tyrosinase, indicating that it can control hyperpigmentation and skin spots.


Acacia sieberiana; topical cream; GC-MS; phenolic composition; skin diseases; antioxidant; tyrosinase inhibition

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Copyright (c) 2023 Alfred Ngenge Tamfu, Alain Yaya Koudoro, Selcuk Kucukaydin, Theophile Olaye, Pascal Dossa Cokou Agbangnan, Dominique Codjo Koko Sohounhloue, Felicien Avlessi

Biology, Medicine, & Natural Product Chemistry
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