Synthesis, Spectroscopic Analysis and Antidiabetic Properties of Copper (II) Complex of Mangifera indica Leaf Crude Extract

Mary Adelaide Oladipo, Folasade Omobolanle Ajao, Adewusi John Adepoju, Kayode Taiwo Ishola, Olalekan Jamiu Ajeigbe


Many applied conventional drugs in treating diabetes have been reported to possess some drawbacks which necessitate a search for alternative therapies. In order to search for a more active antidiabetic agent, this study synthesized and evaluated antidiabetic properties of Mangifera indica crude extract and its Cu (II) complex in alloxan-induced diabetic albino rats. The leaf crude extract and its metal complex were characterized using percentage metal analysis and IR spectroscopic data. Experimental animals were induced by a single intraperitoneal injection of Alloxan monohydrate at a single dose of 140 mg/kg body weight and animals with fasting blood glucose level (BGL) > 200 mg/dL were considered diabetic. Metformin was used as a standard drug. Fasting blood glucose level and body weight were used to assess the antidiabetic activity. One-way ANOVA was used to determine the level of statistically significant at p< 0.05. The crude extract was found to coordinate with the metal ion through O donor atom of C=O and O-H of phenol and ketone respectively. The Cu (II) complex of the crude extracts at tested dose of 600mg/kg demonstrated more antidiabetic activity without weight gain than the standard drug. It is concluded that the Cu (II) complex could be a potential material in the development of more active and negative-side-effect-free antidiabetic drug.


Albino rats; Body weight; Blood glucose; Metal complex

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Adeoye A T, Oyagbemi A A, Adedapo A D, Omobowale T O, Ayodele A E, Adedapo A A (2017). Antidiabetic and antioxidant activities of the methanol leaf extract of Vernonia amygdalina in alloxan-induced diabetes in Wistar ratsJ. med. plant. econ. dev. 1(1): 30-45

Aderibigbe A O, Emudianughe T S, Lawal B A S (2001). Evaluation of the antidiabetic action of mangifera indica in mice, Phytotherapy Research 15 (5): 456458

Andrew O, Yusuf S, Jangabe L M, Lawal B S, Adamu A A (2013). ?-glucosidase inhibitory potential of selected anti-diabetic plants used in north-western Nigeria. Journal of Medicinal Plants Research, 7(27): 2010-2018.

Atangwho I J, Ebong P E, Eyong E U, Eteng M U, Uboh F E (2007). A potential prophylactic anti-diabetic agent in lipid complication. Int. J. Appl. Sci. 18(1):103 106.

Bandaranayake W. M. (2006). Quality control, screening, toxicity, and regulation of herbal drugs, in: I. Ahmad, F. Aqil, and M. Owais (Eds), Modern Phytomedicine Turning. Medicinal Plants into Drugs. Wiley-VCH GmbH & Co. KGaA, Weinheim

Chao EC, Henry R R (2010). SGLT2 inhibitiona novel strategy for diabetes treatment.Nat. Rev. Drug Discov. 9: 551559.

Coates J (2001). Interpretation of Infrared Spectra, A Practical Approach, Encyclopedia o of Analytical Chemistry in R.A. Meyers (Ed.), John Wiley & Sons Ltd, Chichester.

Dineshkumar B, Mitra A, Manjunatha M (2010). Studies on the antidiabetic and hypolipidemic potentials of mangiferin (Xanthone glucoside) in STZ-induced type 1 and type 2 diabetic rat models. Int J Adv Pharm Sci. 1: 7585.

Ganogpichayagrai A, Palanuvej C, Ruangrungsi N (2017) Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves. J Adv Pharm Technol Res., 8:19-24

Garrido-Suarez B B, Garrido G, Castro-Labrada M, Merino N, Valdes O, Rodeiro I, Delgado Hernandez R (2014). Anti-hypernociceptive effect of mangiferin in persistent and neuropathic pain models in rats. Pharmacol. Biochem. Behav. 124, 311319. 10.1016/j.pbb.2014.06.019

Jurca T, Marian E, Vica? L G, Mure?an M E (2017). Metal Complexes of Pharmaceutical Substances, Spectroscopic Analyses - Developments and Applications, in: E. Sharmin Editor and F. Zafar Editor Ed(s)., IntechOpen, London.

Krentz AJ, Bailey CJ (2005) Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs 65: 385411

Mohammed A, Kumar D, Rizvi S I (2015). Antidiabetic potential of some less commonly used plants in traditional medicinal systems of India and Nigeria. J Intercult Ethnopharmacol, 4(1): 78-85. doi: 10.5455/jice.20141030015241.

Patarakijavanic P, Sato V H, Kongkiatpaiboon S, Chewchinda, S. (2019) A review of the antidiabetic potential of Mangifera indica leaf extract. Songklanakarin J. Sci. Technol. 41 (4): 942 950

Pepato M T, Mori D M, Baviera A M, Harami J B, Vendramini R C, Brunetti I L (2005). Fruit of the jambolan tree (Eugenia jambolana Lam.) and experimental diabetes J. Ethnopharmacol 96:43-48

Sabu M C, Subburaj T (2002). Effect of Cassia auriculata Linn. on serum glucose level, glucose utilization by isolated rat hemidiaphragm, J Ethnopharmacol 85: 201-206

Sharma S, Nasir A, Prabhu K M, Murthy P S, Dev G (2003). Hypoglycaemic and hypolipidemic effect of ethanolic extract of seeds of Eugenia jambolana in alloxan-induced diabetic rabbits. J Ethnopharmacol 85: 201-206

Shastr K (1980). Comments on Charaka Samhita. Chanukah Bharati, Varanasi, India.

Upadhyay J., Polyzos S.A., Perakakis N, Thakkar B, Paschou S A, Katsiki N (2018) Pharmacotherapy of type 2 diabetes: An update. Metabolism 78:13-42.

Wadood A, Ghufran M, Jamal S B, Naeem M, Khan A, Ghaffar R (2013). Phytochemical analysis of medicinal plants occurring in local area of Mardan, Biochem Anal Biochem 2: 144. doi: 10.4172/2161-1009.

World Health Organization (WHO)M. (2016). Global report on diabetes. Retrieved from;jsessionid=F E60BB6C97974A6BCD4D02F64F018EA?sequence=1



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Copyright (c) 2023 Mary Adelaide Oladipo, Folasade Omobolanle Ajao, Adewusi John Adepoju, Kayode Taiwo Ishola, Olalekan Jamiu Ajeigbe

Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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