Kidney Evaluation in Hyperuricemia Rats Treated with Green Tea Leaves (Camellia sinensis L.) Extract

Putranty Widha Nugraheni, Chanif Mahdi

Abstract


Uric acid is an oxidation product of the xanthine oxidase enzyme found in extracellular fluid, and when it exceeds, uric acid will build up and cause hyperuricemia. TNF-α is released by epithelial cells and mesangial cells when inflammation occurs and causes apoptosis in epithelial cells, causing damage to kidney structures and initiating acute kidney poisoning. Green tea extract (Camellia sinensis L.) contains many antioxidants, especially flavonoids with potent antioxidant properties such as lipid peroxidase and free radical absorbers, inhibiting xanthine oxidase. This study expresses the potential of green tea extract on kidney repair caused by HUA. Twenty-four male albino rats (175-225 g) of Wistar strain being fed a high purine diet in 60 consecutive days and divided into six groups randomly, I: negative control, II: positive control, III: allopurinol, IV: green tea extract 150mg of body weight, V: green tea extract 300mg of body weight, and VI: green tea extract 600mg of body weight. Treatment was done for 14 days and measured by total creatinine levels, malondialdehyde levels, and kidney histopathology. The statistical analysis using One Way ANOVA and Post Hoc Tukey analysis by SPSS 23.0 proved that green tea extract with a dose of 600 mg/kg of body weight green could lower levels malondialdehyde of the kidney as much as 58.85% (p<0.01), decreased creatinine level by 24.5% (p>0.05), and improved kidney histopathology. This study proved that green tea extract is a promising alternative for hyperuricemia while improving kidney tissues and lowering malondialdehyde and creatinine levels.


Keywords


Camellia sinensis L.; hyperuricemia; kidney creatinine; kidney histopathology; kidney malondialdehyde

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References


Abraham, E. (2003). Nuclear Factor-κB and Its Role in Sepsis-Associated Organ Failure. Journal of Infectious Diseases, 187(Suppl. 2), S364–S369. https://doi.org/10.1086/374750

Angielski, S. (1992). The distribution of enzymes involved in purine metabolism in rat kidney. Biochimica et Biophysica Acta, 3, 1–6.

Arjumand, W., Seth, A., & Sultana, S. (2011). Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NFκB, TNF-α and caspase-3 expression in wistar rats. Food and Chemical Toxicology, 49(9), 2013–2021. https://doi.org/10.1016/j.fct.2011.05.012

Asghar, M., George, L., & Lokhandwala, M. F. (2007). Exercise decreases oxidative stress and inflammation and restores renal dopamine D1 receptor function in old rats. American Journal of Physiology. Renal Physiology, 293(July 2007), F914–F919. https://doi.org/10.1152/ajprenal.00272.2007

Astuti, D. (2011). Efek Antihiperurisemia Kombinasi Ekstrak Air Kelopak Rosella (Hibiscus sabdariffa L) dan Akar Tanaman Akar Kucing (Acalypha indica L) Pada Tikus Putih Jantan yang Diinduksi Putih Jantan yang Diinduksi Kalium Oksonat.

Bolignano, D., Coppolino, G., Barillà, A., Campo, S., Criseo, M., Tripodo, D., & Buemi, M. (2007). Caffeine and the Kidney: What Evidence Right Now? Journal of Renal Nutrition, 17(4), 225–234. https://doi.org/10.1053/j.jrn.2007.02.006

Boudiaf, K., Houcher, Z., Widad, S., & Benboubetra, M. (2010). Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds ’ extracts. Journal of Applied Biological Sciences, 4(1), 7–16.

Boumerfeg, S., Baghiani, A., Djarmouni, M., & Ameni, D. (2012). Inhibitory Activity on Xanthine Oxidase and Antioxidant Properties of Teucrium polium L . Extracts. Chinese Medicine, 2012(March), 30–41.

Cao, H., Pauff, J. M., & Hille, R. (2010). Substrate orientation and catalytic specificity in the action of xanthine oxidase: The sequential hydroxylation of hypoxanthine to uric acid. Journal of Biological Chemistry, 285(36), 28044–28053. https://doi.org/10.1074/jbc.M110.128561

Chen, G., Tan, M. L., Li, K. K., Leung, P. C., & Ko, C. H. (2015). Green tea polyphenols decreases uric acid level through xanthine oxidase and renal urate transporters in hyperuricemic mice. Journal of Ethnopharmacology, 175, 14–20. https://doi.org/10.1016/j.jep.2015.08.043

Cirillo, P., Sato, W., Reungjui, S., Heinig, M., Gersch, M., Sautin, Y., Nakagawa, T., & Johnson, R. J. (2006). Uric acid, the metabolic syndrome, and renal disease. Journal of American Society Nephrology, 17(12 Suppl 3), S165-8. https://doi.org/10.1681/ASN.2006080909

Dahal, A., & Mulukuri, S. (2015). Flavonoids in Kidney Protection. World Journal of Pharmacy and Pharmaceutical Sciences, 4(03), 362–382.

Doehner, W., Jankowska, E. A., Springer, J., Lainscak, M., & Anker, S. D. (2016). Uric acid and xanthine oxidase in heart failure - Emerging data and therapeutic implications. International Journal of Cardiology, 213, 15–19. https://doi.org/10.1016/j.ijcard.2015.08.089

Feig, D. I., Soletsky, B., & Johnson, R. J. (2008). Effect of allopurinol on blood pressure of adolescents with newly diagnosed essential hypertension: a randomized trial. JAMA, 300(8), 924–932. https://doi.org/10.1001/jama.300.8.924

Forester, S. C., & Lambert, J. D. (2011). The role of antioxidant versus pro-oxidant effects of green tea polyphenols in cancer prevention. Molecular Nutrition & Food Research, 55(6), 844–854. https://doi.org/10.1002/mnfr.201000641

Fraga, C. G., Galleano, M., Verstraeten, S. V., & Oteiza, P. I. (2010). Basic biochemical mechanisms behind the health benefits of polyphenols. Molecular Aspects of Medicine, 31(6), 435–445. https://doi.org/10.1016/j.mam.2010.09.006

Goicoechea, M., Vinuesa, S. G. De, Verdalles, U., Ruiz-caro, C., Ampuero, J., Rinco, A., & Arroyo, D. (2010). Effect of Allopurinol in Chronic Kidney Disease Progression and Cardiovascular Risk. Clinical of American Society of Nephrology, 5, 1388–1393. https://doi.org/10.2215/CJN.01580210

Hosten, A. O. (1982). BUN and Creatinine.

Jatuworapruk, K., Srichairatanakool, S., Ounjaijean, S., Kasitanon, N., Wangkaew, S., & Louthrenoo, W. (2014). Effects of green tea extract on serum uric acid and urate clearance in healthy individuals. Journal of Clinical Rheumatology, 20(6), 310–313. https://doi.org/10.1097/RHU.0000000000000148

Kakuda, T., Nozawa, A., Unno, T., Okamura, N., & Okai, O. (2000). Inhibiting Effects of Theanine on Caffeine Stimulation Evaluated by EEG in the Rat. Bioscience Biotechnology Biochemistry, 64(2), 287–293.

Kang, D.-H., Nakagawa, T., Feng, L., Watanabe, S., Han, L., Mazzali, M., Truong, L., Harris, R., & Johnson, R. J. (2002). A Role for Uric Acid in the Progression of Renal Disease. Journal of the American Society of

Nephrology, 13(12), 2888–2897. https://doi.org/10.1097/01.ASN.0000034910.58454.FD

Kang, D., Park, S., Lee, I., & Johnson, R. J. (2005). Uric Acid – Induced C-Reactive Protein Expression : Implication on Cell Proliferation and Nitric Oxide Production of Human Vascular Cells. Journal of American of Society Nephrology, 16(17), 3553–3562. https://doi.org/10.1681/ASN.2005050572

Kramer, H. M., & Curhan, G. (2002). The association between gout and nephrolithiasis: The National Health and Nutrition Examination Survey III, 1988-1994. American Journal of Kidney Diseases, 40(1), 37–42. https://doi.org/10.1053/ajkd.2002.33911

Lago, J. H. G., Toledo-Arruda, A. C., Mernak, M., Barrosa, K. H., Martins, M. A., Tibério, I. F. L. C., & Prado, C. M. (2014). Structure-Activity association of flavonoids in lung diseases. Molecules, 19(3), 3570–3595. https://doi.org/10.3390/molecules19033570

Lelyana, R. (2008). Asam Urat Darah: Studi Eksperimen pada Tikus Rattus norwegicus Galur Wistar. http://eprints.undip.ac.id/19270/1/Rosa_Lelyana.pdf

Lin, J., Chen, P., Ho, C., & Lin-Shiau, S.-Y. (2000). Inhibition of Xanthine Oxidase and Suppression of Intracellular Reactive Oxygen Species in HL-60 Cells by Theaflavin-3 , 3 ‘ - Inhibition of Xanthine Oxidase and Suppression of Intracellular Reactive Oxygen Species in HL-60 Cells by Theaflavin-3 , 3 ′ -di. Journal of Agricultural and Food Chemistry, 48(October), 2736–2743. https://doi.org/10.1021/jf000066d

Lipkowitz, M. S. (2012). Regulation of uric acid excretion by the kidney. Current Rheumatology Reports, 14(2), 179–188. https://doi.org/10.1007/s11926-012-0240-z

Lippi, G., Montagnana, M., Franchini, M., Favaloro, E. J., & Targher, G. (2008). The paradoxical relationship between serum uric acid and cardiovascular disease. Clinica Chimica Acta, 392(1–2), 1–7. https://doi.org/10.1016/j.cca.2008.02.024

Nair, V., Vietti, D. E., & Cooper, C. S. (1981). Degenerative Chemistry of Malondialdehyde, Structure, Stereochemistry, and Kinetics of Formation of Enaminals from Reaction with Amino Acids. Journal of American Chemistry Society, 103(32), 3030–3036.

Noeman, S. A., Hamooda, H. E., & Baalash, A. A. (2011). Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetology & Metabolic Syndrome, 3(1), 17. https://doi.org/10.1186/1758-5996-3-17

Nugraheni, P. W., Rahmawati, F., Mahdi, C., & Prasetyawan, S. (2017). Green Tea Extract (Camellia sinensis L.) Effects on Uric Acid Levels on Hyperuricemia Rats (Rattus norvegicus). The Journal of Pure and Applied Chemistry, 6(3), 246–254.

Paganini-hill, A., Kawas, C. H., & Corrada, M. M. (2007). Non-alcoholic beverage and caffeine consumption and mortality : The Leisure World Cohort Study ☆. Preventive Medicine, 44, 305–310. https://doi.org/10.1016/j.ypmed.2006.12.011

Rahmawati, F., Nugraheni, P. W., Mahdi, C., Srihardyastutie, A., & Prasetyawan, S. (2018). Optimization Of Elevating Blood Uric Acid Levels With High Purine Diet. The Journal of Pure and Applied Chemistry Research, 7(1), 19–25. https://doi.org/10.21776/ub.jpacr.2018.007.01.357

Rashidinejad, A., Birch, E. J., & Everett, D. W. (2016). Green tea catechins suppress xanthine oxidase activity in dairy products: An improved HPLC analysis. Journal of Food Composition and Analysis, 48, 120–127. https://doi.org/10.1016/j.jfca.2016.03.001

Rogers, P. J., Smith, J. E., Heatherley, S. V, & Pleydell-Pearce, C. W. (2008). Time for tea : mood , blood pressure and cognitive performance effects of caffeine and theanine administered alone and together.

Psychopharmacology, 195, 569–577. https://doi.org/10.1007/s00213-007-0938-1

Soobrattee, M. A., Neergheen, V. S., Luximon-Ramma, A., Aruoma, O. I., & Bahorun, T. (2005). Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 579(1–2), 200–213. https://doi.org/10.1016/j.mrfmmm.2005.03.023

Toora, B., & Rajagopaj, G. (2002). Measurement of creatinine by Jaffe ’ s reaction - Determination of concentration of sodium hydroxide required for maximum color development in standard , urine and protein free filtrate of serum. Indian Journal of Experimental Biology, 40(1), 352–354.

Tugcu, V., Ozbek, E., Tasci, A. L. I. I., Kemahli, E., & Bas, M. (2006). Selective nuclear factor k -B inhibitors , pyrolidium dithiocarbamate and sulfasalazine , prevent the nephrotoxicity induced by gentamicin. Journal Compilation BJU International, 98, 680–686. https://doi.org/10.1111/j.1464-410X.2006.06321.x

Zhen, M., Wang, Q., Huang, X., Cao, L., Chen, X., Sun, K., Liu, Y., Li, W., & Zhang, L. (2007). Green tea polyphenol epigallocatechin-3-gallate inhibits oxidative damage and preventive effects on carbon tetrachloride – induced hepatic fibrosis. Journal of Nutritional Biochemistry, 18, 795–805. https://doi.org/10.1016/j.jnutbio.2006.12.016




DOI: https://doi.org/10.14421/biomedich.2022.111.17-26

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