Liver and Renal Cell Damage Following Excess Bee Honey Consumption in Male Wistar Rat

Akpevwoghene Agbatutu, Jerome Ndudi Asiwe, Olusegun Gafar Adebayo

Abstract


Honey is a widely used natural product with several health benefits. However, there is paucity of information on its excessive usage. The present study investigated the effect of excess honey consumption on hepato-renal functions in male wistar rats. Twenty-eight adult male Wistar rats were selected into four groups (n=7) and treated with distilled water (control) and 1ml, 2ml and 3ml of honey respectively for 5 weeks. Thereafter, the animals were euthanised and blood as well as kidney and liver were collected for further studies. There was a significant increase in Creatinine, Bilirubin, Urea AST, ALP, total protein as well as a significant decrease in RBC, WBC, haemoglobin, lymphocyte and PCV. Histology of the liver and kidney revealed a significant degeneration and necrosis in a dose dependent manner. This study suggest that excess honey consumption causes liver and renal cellular damage as well as haematological alterations.

Keywords


Honey; Excess consumption; liver enzyme; electrolyte; renal function; haematological indices; histopathology

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References


Abdelaziz I, Elhabiby MI and Ashour AA (2012). Toxicity of cadmium and protective effect of bee honey, vitamins C and B complex. Human and Experimental Toxicology 1–9.

Abdulrhman M, El-Hefnawy M, Ali R, El-Goud AA (2008). Honey and type 1 diabetes mellitus. In: Liu CP, editor. Type Diabetes – Complications, Pathogenesis, and Alternative Treatments. Croatia: In Tech; 2008.

Adebolu TT (2005). Effect of natural honey on local isolates of diarrhea causing bacteria in Southwestern Nigeria. Afr J Biotechnol. 4:1172–4.

Adesoji A Fasanmade and Oluwakemi T Alabi (2008). Differential effect of honey on selected variables in alloxan-induced and fructose-induced diabetic rats. African Journal of Biomedical Research, Vol. 11 191-196.

Ahmed S, Othman NH (2013). Honey as a potential natural anticancer agent: A review of its mechanisms. Evid Based Complement Alternat Med. 829070.

Al-Qayim M, Ghali L, Al-Azwai T (2014) Comparative effects of propolis and malic acid on hematological parameters of aluminum exposed male rats. Glob J Biosci Biotechnol 3(1):6–11.

AL-Waili N, Boni N (2003). Natural honey lowers plasma prostaglandin concentrations in normal individuals. J Med Food 6:129–133.

Al-Waili NS, Saloom KY, Akmal M, Al-Waili F, Al-Waili TN, Al-Waili AN, Ali A (2006) Honey ameliorates influence of hemorrhage and food restriction on renal and hepatic functions, and hematological and biochemical variables. Int J Food Sci Nutr 57(5–6):353–362.

Al-Waili NS, Saloom KY, Al-Waili TN, Al-Waili AN, Akmal M, Al- Waili FS, Al-Waili HN (2006). Influence of various diet regimens on deterioration of hepatic function and haematological parameters following carbon tetrachloride: a potential protective role of natural honey. Nat Prod Res 20(13):1258–1264.

Armstrong WD and Carr CW. Physiological chemistry: laboratory direction. 3rd ed. Minneapolis, MN: Burges publishing Co, p.75.

Ashrafi S, Mastronikolas S, Wu CD (2005). Use of Honey in Treatment of Aphthous Ulcers IADR/AADR/CADR 83rd General Session. Baltimore, MD., USA: pp. 9–12.

Asiwe JN, Anachuna KK, Moke EG, Sanusi KO, Okonofua DE Omeru O, Fasanmade AA, (2021). High dietary salt intake alleviates fasting blood glucose in streptozotocin-induced diabetic male Wistar rats. Thai J Pharm Sci.,45:172-177.

Asiwe, JN., Kolawole, TA., Anachuna, KK., Ebuwe, EI., Nwogueze, BC., Eruotor, H., Igbokwe V., (2022). Cabbage juice protect against Lead-induced liver and kidney damage in male Wistar rat. Biomarkers, 1-8 (ahead of print) doi:10.1080/1354x.2021.2022210.

Attia WY, Gabry MS, El-Shaikh KA, Othman GA (2008). The anti-tumor effect of bee honey in Ehrlich ascite tumor model of mice is coincided with stimulation of the immune cells. J Egypt Public Health Assoc 15:169–83.

Babacan S, Rand AG (2007). Characterization of honey amylase. J Food Sci.72:C050–5.

Bahrami, M. Ataie-Jafari, A. Hosseini, S. Foruzanfar, M.H. Rahmani, M. Pajouhi, M (2009). Effects of natural honey consumption in diabetic patients: An 8-week randomized clinical trial. Int. J. Food Sci. Nutr. 60, 618–626.

Bansal V, Medhi B, Pandhi P (2005). Honey – A remedy rediscovered and its therapeutic utility. Kathmandu Univ Med J (KUMJ) 3:305–9.

Bartels H, Bohmer M, and Heierli C (1972). Serum creatinine determination without protein precipitation. Clin Chem Acta 37: 193–197.

Bazzoni GB, Bollini AN, Herna´ndez GN, Contini Mdel C, Chiarotto MM, Rasia ML (2005) In vivo effect of aluminum upon thephysical properties of the erythrocyte membrane. J Inorg Biochem 99:822–827

Bessey OA, Lowry DH, Brock JM (1946). Method for the determination of alkaline phosphatase with five cubic milliliters of serum. J Biol Chem. 146: 321.

Betts J. (2008). The clinical application of honey in wound care. Nurs Times.104:43–4.

Candiracci M, Piatti E, Dominguez-Barragán M, García-Antrás D, Morgado B, Ruano D, (2012). Anti-inflammatory activity of a honey flavonoid extract on lipopolysaccharide-activated N13 microglial cells. J Agric Food Chem. 60:12304–11.

Chepulis, L.M (2007). The effect of honey compared to sucrose, mixed sugars, and a sugar-free diet on weight gain in young rats. J. Food Sci. 72, S224–S229.

Dashora N, Sodde V, Bhagat J, Kirti SP, Labo R (2011). Antitumor activity of Dendrophoe falcate against Ehrlich ascites carcinoma in Swiss albino mice. Pharm Crops.7:1.

Doumas BT, Watson WA, and Biggs HG (1971). Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta 31(1):87–96.

Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E (2008). Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem Toxicol. 46:3774–9.

Eteraf-Oskouei T, Najafi M (2013). Traditional and modern uses of natural honey in human diseases: A review. Iran J Basic Med Sci. 16 (6) :731–742.

Fawcett JK Scott JE (1960). A rapid and precise method for determination of urea. J Clin Pathol 13: 156–159.

French VM, Cooper RA, Molan PC (2005). The antibacterial activity of honey against coagulase-negative staphylococci. J Antimicrob Chemother. 56:228–31.

Ghorbel I, Maktouf S, Kallel C, Ellouze Chaabouni S, Boudawara T, Zeghal N (2015). Disruption of erythrocyte antioxidant defense system, hematological parameters, induction of pro-inflammatory cytokines and DNA damage in liver of co-exposed rats to aluminium and acrylamide. Chem Biol Interact 236:31–40.

Ghosh S, Playford RJ (2003). Bioactive natural compounds for the treatment of gastrointestinal disorders. Clin Sci (Lond). 104:547–56.

Hassapidou M, Fotiadou E, Maglara E, Papadopoulou SK (2006). Energy intake, diet composition, energy expenditure, and body fatness of adolescents in Northern Greece. Obesity (Silver Spring) 14:855–62.

Heba M Halawa, Nagwa E El-Nefiawy, Noha A Makhlouf, Awatef A Mady (2009). Evaluation of honey protective effect on Lead induced oxidative stress in rats. Jasmr, 4 (2): 197-208.

Helmy N, El-Soud A (2012). Honey between traditional uses and recent medicine. Maced J Med Sci. 5:205–14.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA. Gelam (2012). honey inhibits the production of proinflammatory, mediators NO, PGE (2), TNF-a, and IL-6 in carrageenan-induced acute paw edema in rats. Evid Based Complement Alternat Med. 2012:109636.

Iglesias MT, De Lorenzo C, Del Carmen Polo M, Martín-Alvarez PJ, Pueyo E (2004). Usefulness of amino acid composition to discriminate between honeydew and floral honeys. Application to honeys from a small geographic area. J Agric Food Chem. 52:84–9.

Islam A, Khalil I, Islam N, Moniruzzaman M, Mottalib A, Sulaiman SA (2012). Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year. BMC Complement Altern Med. 12:177.

Jansen, S.A.; Kleerekooper, I.; Hofman, Z.L.M.; Kappen, I.F.P.M.; Stary-Weinzinger, A.; Van Der Heyden,M.A.G (2012). Grayanotoxin poisoning: “Mad honey disease” and beyond. Cardiovasc. Toxicol. 12, 208–215.

Khalil I, Moniruzzaman M, Boukraâ L, Benhanifia M, Islam A, Islam N (2012). Physicochemical and antioxidant properties of Algerian honey. Molecules. 17:11199–215.

Lay-flurrie K (2008). Honey in wound care: Effects, clinical application and patient benefit. Br J Nurs. 17:S30, S32–6.

Li, S.; Shin, H.J.; Ding, E.L.; van Dam, R.M (2009). Adiponectin levels and risk of type 2 diabetes: A systematic review and meta-analysis. JAMA 302, 179–188.

Manyi-Loh CE, Clarke AM, Ndip RN (2011). Identification of volatile compounds in solvent extracts of honeys produced in South Africa. Afr J Agric Res. 6:4327–34.

Mato I, Huidobro JF, Simal-Lozano J, Sancho MT (2003). Significance of nonaromatic organic acids in honey. J Food Prot. 66:2371–6.

Moore, O.A., Smith, L.A., Campbell, F., Seers, K., McQuay, H.J., and Moore, R.A. (2001). Systematic review of the use of honey as a wound dressing. BMC Complement. Altern. Med. 1, 2.

Murugavel P and Pari L (2007). Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats. Hum Exp Toxicol 26: 527–534.

Onyije F.M, O.G. Avwioro, A.D. Atoni1, A. Nduku and V.C. Zenebo (2011). Evaluation of the Kidney Following Administration of Unprocessed Honey in Wistar Rats. Asian J. Med. Pharm. Res. 2 (1): 21-25.

Özta¸san, N. Altinkaynak, K. Akçay, F. Göçer, F. Dane, ¸S (2005). Effects of mad honey on blood glucose and lipid levels in rats with streptozocin-induced diabetes. Turk. J. Vet. Anim. Sci., 29, 1093–1096.

Pataca LC, Borges Neto W, Marcucci MC, Poppi RJ (2007). Determination of apparent reducing sugars, moisture and acidity in honey by attenuated total reflectance-Fourier transform infrared spectrometry. Talanta. 71:1926–31.

Perry PW, Doumas BT, and Bayse DD (1983). A candidate reference method of determination of bilirubin in serum: test for transferability. Clin Chem. 29: 297.

Rashed MN, Soltan ME (2004). Major and trace elements in different types of Egyptian mono-floral and non-floral bee honeys. J Food Compos Anal. 17:725–735.

Reitman S and Frankel S (1957). A colorimetric method for the glutamic–pyruvate transaminase. Am J Clin Pathol 28: 56–63.

Sato T, Miyata G 2000. The nutraceutical benefit, part iii: Honey. Nutrition. 16:468–9.

Vorlova L, Pridal A (2002). Invertase and diastase activity in honeys of Czech provenience. Acta Univ Agric. 5:57–66.

White JW 1980. Detection of honey adulteration by carbohydrate analysis. J Assoc Off Anal Chem. 63:11–8.

Wilson JI, George BO, Umukoro GE (2011). Effects of honey on the histology of liver in adult Wistar rats. Biology and Medicine, 3 (1): 1-5.

Yaghoobi R, Kazerouni A, Kazerouni O, (2013). Evidence for clinical use of honey in wound healing as an anti-bacterial, anti-inflammatory anti-oxidant and anti-viral agent: A review. Jundishapur J Nat Pharm Prod. 8:100–4.

Yaghoobi, N. Al-Waili, N Ghayour-Mobarhan, M. Parizadeh, S.M.R. Abasalti, Z. Yaghoobi, Z. Yaghoobi, F Esmaeili, H. Kazemi-Bajestani, S.M.R. Aghasizadeh, R (2008). Natural honey and cardiovascular risk factors; effects on blood glucose, cholesterol, triacylglycerole, CRP, and body weight compared with sucrose. Sci. World J. 8, 463–469.




DOI: https://doi.org/10.14421/biomedich.2022.111.35-43

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Copyright (c) 2022 Akpevwoghene Agbatutu, Jerome Ndudi Asiwe, Olusegun Gafar Adebayo



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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