Acute Toxicity and Hypoglycemic Effect of a Polyherbal Formulation on Blood Glucose in Oral Glucose Tolerance Test (OGTT) and Alloxan-Induced Diabetic Rats

Abednego Okeoghene Warri, Emuesiri Goodies Moke, Aishat Oyinkansola Balogun, Kennedy Chibogu Nzeh, Emuesiri Kohworho Umukoro, Earnest Oghenesuvwe Erhirhie


Madam F. Kayes Bitters® is an herbal formulation commonly used in Nigeria and some African countries in the management of diabetes mellitus and other diseases conditions. This study evaluated the in-vivo hypoglycaemic activity, as well as acute toxicity of the polyherbal formulation to provide its efficacy and safety. Healthy albino mice (20-30 g) and Sprague Dawley female rats (90-130 g) were used for this study. Acute toxicity study (LD50) of the herbal formulation was determined by methods originally described by Miller and Tainter in 1994. Following oral dosing with glucose (2 g/kg) in normal fasted animals, herbal formulation (HF) at various doses was administered and blood glucose levels at 30 minutes, 60 minutes, 90 minutes, and 120 minutes were taken and recorded. Diabetes was induced using alloxan 150 mg/kg and diabetic rats were given the HF at doses of 50, 100, and 200 mg/kg with glibenclamide 2.5 mg/kg used as standard drug treatment. Blood glucose level was determined on 1st day, 7th day, 14th and 21st day. The LD50 was greater than 5g/kg with oral administration. The oral glucose tolerance test showed that the group that received 100 mg/kg HF showed a significant reduction (p<0.05) in glucose level after 120 minutes when compared to the basal level of glucose recorded. All treated diabetic groups showed a significant decrease in glucose level on the 21st day. The herbal formulation of Hydrastis canadesis Aloe capensis, Echinacea angustifolia and honey exhibited a significant glucose-lowering activity in alloxan-induced diabetic rats.


alloxan monohydrate; diabetes mellitus; herbal formulation; LD50 value

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Abo KA, Fred-Jaiyesimi AA, Jaiyesimi AE (2008). Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in South Western Nigeria. J Ethnopharmacol 115(1): 67-71.

Abubakar US, Abdullahi S, Ayuba V, Kaigama S, Halidu US, Ayuba MK (2017). Medicinal Plants Used for the Management of Diabetes Mellitus in Zaria, Kaduna State, Nigeria. J Pharm Pharmacogn Res 5(3): 156–164.

Adeneye AA, Agbaje EO (2008). Pharmacological Evaluation of Oral Hypoglycaemic and Antidiabetic Effects of Fresh Leaves Ethanolic Extract of Morinda Lucida Benth. in Normal and Alloxan-induced Diabetic Rats. Afr J Biomed Res 11: 65-71.

American Diabetes Association (2009). Diagnosis and classification of diabetes mellitus. Diabetes Care 32(Suppl 1): S62-7.

Anachuna KK, Oyem CJ, Nwogueze BC, Asiwe JN (2018). Glucose lowering effects and histomorphological changes of Vernonia amygdalina on pancreatic compromised Wistar rats using alloxan monohydrate. Trop J Health Sci 25(2): 27-31.

Asmat U, Abad K, Ismail K (2016). Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J 24(5): 547-553.

Choudhary M, Kochhar A, Sangha J (2014). Hypoglycemic and hypolipidemic effect of Aloe vera L. in non-insulin dependent diabetics. J Food Sci Technol 51(1): 90-6.

Da Silva Xavier G (2018). The Cells of the Islets of Langerhans. J Clin Med 7(3): 54.

Ekor M (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 4: 177.

Erejuwa OO, Sulaiman SA, Wahab MS (2012). Honey - a novel antidiabetic agent. Int J Biol Sci 8(6): 913-34.

Erejuwa OO (2014). Effect of honey in diabetes mellitus: matters arising. J Diabetes Metab Disord 13(1): 23.

Erejuwa OO (2012). Management of diabetes mellitus: could simultaneous targeting of hyperglycemia and oxidative stress be a better panacea? Int J Mol Sci 13(3): 2965-72.

Erhirhie EO, Ilodigwe EE, Ajaghaku DL, Mbagwu IS, Moke EG (2015). Toxicity Evaluation of a Commercial Herbal Preparation Commonly Used in Nigeria. European J Med Plants 5(2): 176-190.

Erhirhie EO, Ihekwereme CP, Ilodigwe EE (2018). Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdiscip Toxicol 11(1): 5-12.

Ezuruike UF, Prieto JM (2014). The use of plants in the traditional management of diabetes in Nigeria: pharmacological and toxicological considerations. J Ethnopharmacol 155(2): 857-924.

Gbolade AA, Ekor MN, Akinlolu AA, Ayoola MD (2008). Antidiabetic activity of stem bark ethanolic extracts of Spondias mombin on alloxan-induced diabetic rats. J Pharmaceut Res 7: 192-195.

Gurib-Fakim A (2006). Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27(1): 1-93.

Hammeso WW, Emiru YK, Ayalew Getahun K, Kahaliw W (2019). Antidiabetic and Antihyperlipidemic Activities of the Leaf Latex Extract of Aloe megalacantha Baker (Aloaceae) in Streptozotocin-Induced Diabetic Model. Evid Based Complement Alternat Med 2019: 8263786.

Kitabchi AE, Umpierrez GE, Miles JM, Fisher JN (2009). Hyperglycemic crises in adult patients with diabetes. Diabetes Care 32(7): 1335-43.

Lahlou M (2013). The success of natural products in drug discovery. Pharmacol Pharm 4: 17-31.

Moke EG, Ilodigwe EE, Okonta JM, Emudainohwo JOT, Ajaghaku DL, Erhirhie OE, Chinwuba P, Ahante E (2015). Antidiabetic Activity and Toxicity Evaluation of Aqueous Extracts of Spondias mombin and Costus afer on Wistar Rats. Br J Pharm Res 6(5): 333-342.

Moke EG, Mordi JC, Umukoro EK (2020). Effects of Methanol Leaf Extract of Cuphea Hyssopifolia Kunth on Liver Enzymes Activity and Antioxidant Indices of Paracetamol-Induced Hepatotoxicity in Wistar Rats. Afr J Biomed Res 23(1): 123-6.

Moke EG, Umukoro EK, Asiwe JN, Omogbiya AI, Erhirhie EO, Ben-Azu B, Anieh FU (2021). Herbal Medicine: Education and Occupation Influences Its Practice among Residents of Port Harcourt, South-South Nigeria. Int J Pharm Phytopharm Res 11(2): 38-44.

Nwanjo HU (2006) Antioxidant activity of the exudates from Aloe barbadensis leaves in diabetic rats. Biokemistri 18(2): 77-81.

Okafo SE, Moke EG, Obi CS (2019). Formulation and evaluation of anti-diabetic tablets containing aqueous extract of Moringa oleifera seeds. JOPHAS 16(5): 3167-3176.

Ozougwu JC, Obimba KC, Belonwu CD, Unakalamba CB (2013). The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. J Physiol Pathophysiol 4(4): 46-57.

Pothuraju R, Sharma RK, Onteru SK, Singh S, Hussain SA (2016). Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review. Phytother Res 30(2): 200-7.

Sofowora A, Ogunbodede E, Onayade A (2013). The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med 10(5): 210-29.

Veeresham C (2012). Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res 3(4): 200-1.

Vickers A, Zollman C, Lee R (2001). Herbal medicine. West J Med 175(2): 125-8.



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Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
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