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The Effectiveness of Sambiloto Leaf (Andrographis paniculata) as an Immunostimulant Phytobiotic on Erythrocyte, Hemoglobin, and Hematocrit Levels in Broiler Chickens
Abstract
Andrographis paniculata (Sambiloto) as an immunostimulant phytobiotic is effective on the erythrocyte, hemoglobin, and hematocrit levels of broiler chickens. The objective of this study was to determine the effectiveness of sambiloto leaves as a phytobiotic on the erythrocyte, hemoglobin, and hematocrit levels of broilers. The materials used were 50 male Day-Old Chickens (DOC) of the MB 202 strain from PT. Japfa Comfeed Indonesia, Comfeed feed, sambiloto leaves, 3 ml syringes, vacutainer tubes containing EDTA anticoagulant, 70% alcohol swabs, and a Hematology Analyzer for examining hematological parameters. The research method was experimental, utilizing a Completely Randomized Design (CRD). There were 5 treatments, with 4 replications for each treatment. The treatments consisted of P0 as a negative control without sambiloto, P1 as a positive control with vitamins but without sambiloto, P2 with 5% sambiloto, P3 with 10% sambiloto, and P4 with 15% sambiloto. The treatments were administered from day 14 for a duration of 21 days. The observed variables were erythrocytes, hemoglobin, and hematocrit. The obtained data were analyzed using Analysis of Variance (ANOVA), followed by Duncan's Multiple Range Test to determine differences between treatments. The results showed that the inclusion of sambiloto powder in the feed had a highly significant effect (P<0.01) on broiler erythrocytes and hemoglobin, and a significant effect (P<0.05) on hematocrit. The highest average erythrocyte level in broilers was found in the 5% sambiloto treatment, at 12.38 x106/mm3. The highest average hemoglobin level was also in the 5% sambiloto treatment, at 17.12 g/dl. The highest average hematocrit percentage was in the 5% sambiloto treatment, at 67.69%. In conclusion, sambiloto leaves are effective as a phytobiotic on erythrocyte, hemoglobin, and hematocrit levels, with the 5% dose of sambiloto providing the best production value.
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DOI: https://doi.org/10.14421/biomedich.2026.152.2011-2016
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