Analysis of Flavonoid and Tannin Contents in Pumpkin Seed (Cucurbita moschata) Extract

Adi Fahri, Sutrisnawati Sutrisnawati, Manap Trianto, Zulaikhah Dwi Jayanti, Fatmah Dhafir, Masrianih Masrianih

Abstract


Pumpkin is one of the herbal plants that possesses various medicinal properties, including antidiabetic, antifungal, antibacterial, anti-inflammatory, and antioxidant activities, due to the presence of bioactive compounds in its seeds. This study aimed to identify the bioactive compounds present in pumpkin seed extract using qualitative and quantitative descriptive methods. Qualitative analysis was conducted through color reaction tests using several specific reagents to identify the presence of flavonoid and tannin compounds. Quantitative analysis was performed using the UV-Vis spectrophotometric method based on standard solution calibration curves to determine the concentration of each compound. The qualitative test results indicated that the pumpkin seed extract positively contained flavonoids and tannins, as evidenced by the formation of specific color changes in each test. The quantitative analysis showed that the average flavonoid content was 9.34 mg/g, while the average tannin content was 9.42 mg/g. Based on the results of this study, it can be concluded that pumpkin seed extract contains high levels of flavonoid and tannin compounds, indicating its potential as a natural source of antioxidants. This study is expected to serve as a basis for further development regarding the potential utilization of pumpkin seeds with pharmacological effects.


Keywords


Pumpkin seeds; Flavonoids; Tannins; Qualitative analysis; UV-Vis spectrophotometry

Full Text:

PDF

References


Aditiya, A. P., & Ismawati, R. (2023). Uji Sensori, Kandungan Gizi, dan Nilai Ekonomi Cookies yang Disubstitusi Tepung Kacang Merah dan Tepung Biji Labu Kuning sebagai Snack Tinggi Zat Besi. Jurnal Gizi Universitas Negeri Surabaya, 3 (2), 297–305.

Arifah, F. N., Fitria, F., & Putri, D. E. (2023). Analisis kadar tanin pada ekstrak etanol daging buah maja (Aegle marmelos (L.) Corr)) asal mlati mojo kediri. Jurnal Pharma Bhakta, 3 (2), 66–73.

Basri, R., Abidin, Z., & Pratama, M. (2023). Penetapan Kadar Tanin Ekstrak Etanol Biji Alpukat (Persea americana Mill .) Dengan Menggunakan Metode Spektrofotometri UV-Vis. Makassar Natural Product Journal, 1 (2), 125–137.

Elvira, E., Abidin, Z., & Razak, R. (2024). Analisis Kandungan Flavonoid Ekstrak Etanol Daun Kenikir (Cosmos caudatus). Makassar Pharmaceutical Science Journal, 1 (37), 347–357.

Hakim, A. R., & Saputri, R. (2020). Narrative Review: Optimasi Etanol sebagai Pelarut Senyawa Flavonoid dan Fenolik. Jurnal Surya Medika, 6 (1), 177–180. DOI : 10.33084/jsm.v6i1.1641

Hari, D. G., Lovena, T. N., & Khairiah, U. (2025). Penetapan Kadar Flavonoid Ekstrak Etanol Daun Labu Kuning (Cucurbita moschata Dulchesne) Menggunakan Spektrofotometri Uv-Vis Determination of Flavonoid Content of Ethanol Extract of Yellow Pumpkin Leaves ( Cucurbita moschata Dulchesne ) Using Uv-Vis Spe. Jurnal Farmasimed (JFM), 8 (1), 191–197.

Hartati, M., & Noer, S. (2020). Penetapan kadar senyawa tanin kulit bawang merah (Allium ascalonicum L.). Journal Sinasis, 1 (1), 165–168.

Hidayah, H., Sinangling, B. A., Mulyawan, I., & Kholisoh, T. (2023). Aktivitas Kandungan Flavonoid Jamun (Syzygium cumini) Sebagai Senyawa Anti Inflamasi. Journal Of Social Science Research Volume, 3 (2), 10790–10796.

Hidjrawan Yusi. (2018). Identifikasi Senyawa Tanin pada Daun Belimbing Wuluh (Averrhoa bilimbi L.) No Title. Jurnal Optimalisasi Volume, 4 (2), 78–82. DOI : 10.35308/JOPT.V4I2.1475

Maria, R. D., & Devi, A. (2019). Pengaruh Pemberian Rebusan Daun Pucuk Labu Kuning Terhadap Peningkatan Kadar Hb Ibu Hamil TM III. Jurnal Kesehatan, 10 (1), 27. DOI : 10.35730/jk.v10i1.377

Ningrum, M. W., Nuryanti, S., & Info, A. (2022). Analisis Kadar Senyawa Flavonoid Pada Daun Bawang Hutan (Eleutherine palmifolia (L.) Merr.). Media Eksakta, 18 (2), 150–154.

Noer, S., Pratiwi, R. D., & Gresinta, E. (2018). Penetapan Kadar Senyawa Fitokimia (Tanin, Saponin dan Flavonoid) sebagai Kuersetin Pada Ekstrak Daun Inggu (Ruta angustifolia L.). Jurnal Eksakta, 18 (1), 19–29. DOI : 10.20885/eksakta.vol18.iss1.art3

Nurjannah, I., Mustariani, B. A. A., & Suryani, N. (2022). Skrining Fitokimia Dan Uji Antibakteri Ekstrak Kombinasi Daun Jeruk Purut (Citrus hystrix) Dan Kelor (Moringa oleifera L.) Sebagai Zat Aktif Pada Sabun Antibakteri. Spin Jurnal Kimia & Pendidikan Kimia, 4 (100), 23–36. DOI : 10.20414/spin.v4i1.4801

Nuryanti, S., Fitriana, F., & Ohorella, F. A. (2023). Aktivitas Antibakteri Isolat Fungi Endofit Biji Labu Kuning (Cucurbita moschata ex. Poir). As-Syifaa Jurnal Farmasi, 15 (2), 158–164. DOI : 10.56711/jifa.v15i2.1069

Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5 (47). 1-15. DOI : 10.1017/jns.2016.41

Pangisian, J., Sangi, M. S., & Kumaunang, M. (2022). Analisis Senyawa Metabolit Sekunder dan Uji Aktivitas Antioksidan serta Antibakteri Biji Buah Pangi (Pangium edule Reinw). Jurnal LPPM Bidang Sains Dan Teknologi, 7 (1), 11–19.

Pelu, A. D., Ira P. Ely, & Lukman La Bassy. (2020). Skrining Fitokimia Dan Uji Aktivitas Ekstrak Etanol Biji Labu Kuning (Cucurbita moschata) Terhadap Daya Hambat Bakteri Staphylocuccus aureus. Jurnal Sains Dan Kesehatan, 4 (1), 61–70. DOI : 10.57214/jusika.v4i1.151

Pratama, M., Razak, R., & Rosalina, V. S. (2019). Analisis Tanin Total Ekstrak Etanol Bunga Cengkeh (Syzgium aromaticum L.) Menggunakan Metode Spektrofotometri UV-Vis. Jurnal Fitofarmaka Indonesia, 6 (2), 368–373. DOI : 10.33096/jffi.v6i2.510

Qamarani, Q., & Aryani, A. (2023). Potensi Senyawa Flavonoid sebagai Pengobatan Luka. Jurnal Riset Farmasi, 3 (2), 69–74. DOI : 10.29313/jrf.v3i2.3113

Qodri, U. L. (2023). Pengukuran ?-Karoten pada Daging Labu Kuning (Cucurbita Moschata Durch) Menggunakan Pelarut Etanol, Metanol dan Heksan. Jurnal Syntax Admiration, 4 (7), 989–999. DOI : 10.46799/jsa.v4i7.731

Rahmat, S., & Hairani, S. P. (2025). Identifikasi Senyawa Metabolit Sekunder Sebagai Senyawa Kompleks pada Tanaman Tradisional. Jurnal Ilmu Komputer Dan Ilmu Pengetahuan Alam, 3 (5), 17–26. DOI : 10.62383/polygon.v3i5.747

Ramadhani, M. A., Sunnah, I., & Mardiyanti, D. (2024). Analisis Flavonoid Total Fraksi N-Heksan Ekstrak Biji Labu Kuning ( Cucurbita moschata ) Secara Spektrofotometri UV-VIS. Journal of Holistics and Health Sciences, 6 (2), 307–317. DOI : 10.35473/jhhs.v6i2.501

Udayani, U., Ratnasari, R., & Nida, N. (2022). Penetapan Kadar Senyawa Fitokimia (Alkaloid, Flavonoid dan Tanin) pada Ekstrak Etanol Rimpang Kunyit Hitam (Curcuma caesia Roxb.). Jurnal Pendidikan Tambusai, 6 (1), 2088–2093.

Wahid, R. A. H. (2020). Analisis Kualitatif dan Kuantitatif Tanin Ekstrak Kulit Buah Delima Putih (Punica Granatum L.) Menggunakan Metode Kromatografi Cair Kinerja Tinggi (KCKT). Indonesian Journal of Pharmacy and Natural Product, 3 (2). DOI : 10.35473/ijpnp.v3i2.538

Wildan, A. A., Hayyan, M. A., Naufal, R., Akifurahman, S., & Makassar, M. A. N. K. (2024). Ekstrak Biji Labu Kuning sebagai Biodisinfektan dalam Sabun Cuci Piring Pencegah Infeksi Sistem Kekebalan pada Balita. Prosiding Seminar Nasional Pembangunan Dan Pendidikan Vokasi Pertanian, September, 7 (3), 681–690. DOI : 10.47687/snppvp.v5i1.1018




DOI: https://doi.org/10.14421/biomedich.2026.151.1003-1009

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2026 Adi Fahri, Sutrisnawati Sutrisnawati, Manap Trianto, Zulaikhah Dwi Jayanti, Fatmah Dhafir, Masrianih Masrianih



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC