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Phytochemical Screening and Antioxidant Studies of Trigonella foenum-graecum Seed Extract
Abstract
Drugs are available to combact free radicals and other health related diseases. Fenugreek is one of the medicinal plants to check radicals and other health related diseases. The bioactive compounds in Trigonella foenum-graecum seed were extracted using Methanol through cold merceration technique. The phytochemical screening and antioxidant activities were carried out on the seed using standard methods. The results of phytochemical analysis carried out on the Methanol seed extract of Fenugreek indicates flavonoid, phenol, terpenoid, alkaloids, tannin, Glycoside, Fatty acid, phenols and saponins. However, the IC50 details was evaluated using DPPH free radical. The result of antioxidant activity of the ethylacetate extract exhibited high inhibition at low concentration of 0.1520 mg/cm3 of DPPH while low inhibition of chloroform extract at high concentration of 0.4816 mg/cm3.
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Akbari, S., Abdurahman, N.H., Yunus, R.M., Alara, O.R. & Abayomi. O.O (2019). Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil. Materials Science for Energy Technologies. 2: 349-355.
Alara, O.R., Abdurahman, N.H., Ukaegbu., & Abdulmudalip, S. K. (2017). Effect of drying methods in the free radicals scavenging activity of Vernonia amgdalina in Malaysia. J King Saud Univ-Sci.
Amr Amin, AyshaAlkaabi, Shamaa Al-Falasi, Sayel, & Daoud, A. (2005). “Chemopreventive activities of T. foenum-graecum (Fenugreek) against breast cancer Emirates” Cell Biology International. 29, pp.687-694
Basch, E., Ulbricht, C., Kuo, G., Szapary, P. & Smith M. (2003). Therapeutic applications of fenugreek. Alter Medi Rev;8(1):20-27.
Benzidia, B., Barbouchi, M., Hammouch, H., Belahbib, N., Zouarhi, M., Erramli, H., Ait Daoud, N., Badrane, N. & Hajjaji, N. (2018). Chemical composition and antioxidant activity of tannins extract from green rind of Aloe vera (L.) Burm. F., J. King Saud Univ. - Sci, https://doi.org/10.1016/j.jksus.2018.05.022.
Colombo, M.L. & Bosiosio. E. (1996). “Pharmacological Activities of Chelidonium majus” L (Papaveraceae). Pharmacological Resource. 33: 127-134
Crow, J. M. (2008): Cancer drugs for the next decade unveiled. Chemistry World, 32 (12): 21.
Flores, G. R., Martínez, H. H., Tamez, P. G., Guerra, R. T., Licea, R. Q. & Ramiro. (2010). Antitumor and immunomodulating potential of Coriandrum sativum, Piper nigrum and Cinnamomum zeylanicum. Journal of Natural Products (India), 3: 54-61.
Harbourne, J. B. (1988): Phytochemical Methods, A guide to Modern Techniques of Plant Analysis”, 2nd ed, New York, Chapman and Hall, 4-193.
Jagessar, M. R.C. & Gomes A. G. (2008): An evaluation of the antibacterial and antifungal activity of leaf extracts of Momordica Charantia against Candida albicans, Staphylococcus aureus and Escheria coli. Nature and Science, 6 (1): 1-14
Kukic, J., Popovic, V., Petrovic, S., Mucaji, P., Ciric, A., Stojkovic, D. & Sokovic, M. (2008). Antioxidant and antimicrobial activity of Cnaracar dunculu sex tracts. Food Chemistry, 107 (2): 861-868.
Kumar, N., Ahmad, A.H., Singh, S.P., Pant, D., Prasad, A. & Rastogi, S.K. (2021). Phytochemical analysis and antioxidant activity of Trigonella foenum-graecum seeds. Journal of Pharmacognosy and Phytochemistry; 10(1): 23-26
Manga, H.M., Haddad, M., Pieters, L., Penge, A. & Leclercq, J.Q. (2006): Anti-inflammatory compounds from leaves and root bark of Alchornea cordifolia. Journal of Ethnopharmacology, 115 (1): 25-29
Megha, D., Anissa, M., Balkrishna, U., & Rohini K. (2012). Effect of Trigonella foenum-graecum (Fenugreek/ Methi) on Hemoglobin Levels in Females of Child Bearing Age. Biomedical Research; 23(1):47-50.
Moniruzzaman, Shahinuzzaman, Haque, A., Khatun, R. & Yaakob, Z. (2015). Gas chromatography mass spectrometry analysis and in vitro antibacterial activity of essential oil from Trigonella foenum-graecum S. Asian Pacific Journal of Tropical Biomedicine. http://dx.doi.org/10.1016/j.apjtb.2015.09.010
Nwauzoma, A.B., Chondhary, M.I., Ali, Z. & Olaiya, O,C. (2013). Biological activity of crude extracts of citrus species from Nigeria. Nature and Science: 11(7): 135-139.
Nile, H. S. & Khobragade, C.N. (2010): Antioxidant activity and flavonoid derivatives of Plumbago zeylanica. Journal of Natural Products, 3 (16): 130-133.
Okwute, S.K. (1989). Unusual sources of antibacterial substances. A survey of some Nigerian plants. Nigeria Defence Academy journal. 1:133-141.
Pang, Y., Ahmed, S., Xu, Y., Beta, T., Zhu, Z., Shao, Y. & Bao J. (2018). Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice. Food Chem; 240:212 -221
Pawar, V.S. & Hagar, S. (2012). Adaptogenic activity of Trigonella foenum-gracum (Linn) seeds in rodents exposed to anoxia and immobilization stress. Asian Pacific Journal of Tropical Biomedicine. 2(1); S208-S211
Prasad, N. R., Viswanathan, S., Devi, R. J., Nayak, V., Swetha, C.V., Archana, R.B., Rajkumar, P, J. & Rajkumar, J. (2008): Preliminary Phytochemical screening and antimicrobial activity of Samanea saman. Journal of Medicinal Plants Research, 2 (10): 268-270
Pundarikakshudu, K., Shah, D.H., Panchal, A.H., & Bhavsar, G.C.(2016). Anti-inflammatory Activity of Fenugreek (Trigonellafoenum-graecum Linn) Seed Petroleum Ether Extract. Indian Journal of Pharmacology; 48(4):441-444.
Renuka C. (2009). Evaluation of the antidiabetic effect of Trigonellafoenum-graecum seed powder on alloxan induced diabetic albino rats, International Journal of Pharmaceutical and Technical Research.;1:4:1580-1584.
Szymanska, R., Pospisil, P. & Kruk, J. (2016). Plant derived antioxidants in disease prevention. Oxid Medi Cell Longe ID: 1920208.
Woldemichael M.G., Wachter G., Singh P. M., Maiese M., William, T. & Barbara, N. (2003). Antibacterial Diterpenes from Calceolaria pinifolia. J. Nat. Prod., 66 (2): 242-246.
Yadav, R., Kaushik, R., & Gupta, D. (2011). “The health benefit of Trigonella foenum graecum: A Review” International Journal of Engineering Research and Applications (IJERA). 1, pp.032-035.
Zameer, S., Najmi, A. K., Vohora, D. & Akhtar M. (2017). A review on therapeutic potentials of Trigonella foenum graecum (fenugreek) and its chemical constituents in neurological disorders: Complementary roles to its hypolipidemic, hypoglycemic, and antioxidant potential. Nutr Neurosci;21(8):539-545.
DOI: https://doi.org/10.14421/biomedich.2026.151.1737-1741
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