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The Effect of Methanol Concentrations Against Phenolic Total Content and Antioxidant of Balakacida Leaves (Chromolaena odorata)
Abstract
The balakacida plant with the Latin name Chromolaena odorata is a plant that lives in a tropical climate. The balakacida plant is a plant of the Asteraceae Family. Phytochemical tests on balakacida leaves showed the presence of secondary metabolites of the tannin, phenol, flavonoid, saponin and steroid groups. This study aims to extract balakacida leaves and test the total phenolic and antioxidant content. Sample extraction was carried out by the maceration method using methanol solvent. Testing of total phenolic content using the Folin Ciocalteu method, while testing of antioxidant content using the DPPH method using a UV-Vis spectrophotometer. The results showed that the concentration of methanol solvent significantly affected the acquisition of total phenolics and antioxidants. The highest total phenolic content was obtained at a methanol extract concentration of 80% of 145.64 mg GAE/g. For antioxidant content, a methanol extract concentration of 60% showed the highest activity with an average DPPH % inhibition of 59.77%. It can be concluded that a methanol extract concentration of 60% is the optimal concentration to produce balakacida leaf extract with the best antioxidant activity, although not with the highest total phenolic content.
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Agustin, A., Widayanti, E., Ikayanti, R., Kesuma, S., Prodi, ), Farmasi, A., Makanan, D., Kesehatan, P., & Malang, K. (2022). Penetapan Kadar Fenol Total Ekstrak Etanol Berbagai Biji Buah Salak Bali (Salacca zalanca var. ambonensis) Menggunakan Metode Folin Ciocalteu Total Phenolic Content Determination of Ethanolic Extract from Various Salak Bali Seeds (Salacca zalanca var. ambonensis) Using the Folin Ciocalteu Method. Jurnal Nutriture, 1(3), 19–25.
Allo, I. S., Suryanto, E., & Koleangan, H. S. J. (2022). Aktivitas Antioksidan Fenolik Bebas Dan Terikat Dari Dari Tepung Cangkang Pala (Myristica fragrans Houtt). Chemistry Progress, 15(2), 83–92. https://doi.org/10.35799/cp.15.2.2022.44496
Amalia Rachmawati, R., Wisaniyasa, N. W., & Suter, I. K. (2020). Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Meniran (Phyllanthus niruri L.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 9(4), 458. https://doi.org/10.24843/itepa.2020.v09.i04.p10
Amin, A., Waris, R., Aminah, A., Yuliana, D., & Fitri, N. (2024). Hubungan Kemampuan Aktivitas Antioksidan dengan Pelarut Ekstraksi Daun Pecut Kuda (Stachytarpheta jamaicensis L.). Journal of Pharmaceutical and Health Research, 5(3), 33–41. https://doi.org/10.47065/jharma.v5i3.5221
Ayumi, S. T., Zakiah, Z., & Linda, R. (2023). Potensi Ekstrak Daun Kirinyuh (Chromolaena odorata L.) Sebagai Biostimulan Terhadap Perkecambahan Kacang Tanah (Arachis hypogaea L.). Protobiont, 12(1), 28–33.
Dewantara, L. A. R., Ananto, A. D., & Andayani, Y. (2021). Penetapan Kadar Fenolik Total Ekstrak Kacang Panjang (Vigna unguiculata) dengan Metode Spektrofotometri UV-Visible. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 2(1), 102. https://doi.org/10.31764/lf.v2i1.3759
Fatah, M. I., Muldiyana, T., & Kusnadi, K. (2024). Pengaruh Konsentrasi Pelarut Terhadap Aktivitas Antioksidan Sediaan Serum Ekstrak Kulit Buah Naga Merah (Hylocereus Polyrhizus). JIFI (Jurnal Ilmiah Farmasi Imelda), 7(2), 61–70. https://doi.org/10.52943/jifarmasi.v7i2.1562
Fatmawati, I. S., Haeruddin, & Mulyana, W. O. (2023). Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Belimbing Wuluh (Aveerrhoa bilimbi L.) dengan Metode DPPH. Sains: Jurnal Kimia Dan Pendidikan Kimia, 12(1), 41–49. http://sains.uho.ac.id/index.php/journal
Fertiasari, R., & Kristiandi, K. (2024). Identifikasi Kandungan Antioksidan Pada Teh Celup Kemasan. Journal of Food Security and Agroindustry, 2(2), 69–73. https://doi.org/10.58184/jfsa.v2i2.369
Firdiyansyah, M. R., Sakti, A. S., & Kusumo, D. W. (2023). Review Article : Aplikasi Sejumlah Metode Ekstraksi Modern Untuk. Lumbung Farmasi; Jurnal Ilmu Kefarmasian, 13(2), 1–11.
Hakim, A. R., Nayaken, P. O., & Alawiyah, T. (2023). Pengaruh Metode Ekstraksi terhadap Kadar Alkaloid Total Ekstrak Daun Kirinyuh (Chromolaena odorata). Indonesian Journal of Pharmacy and Natural Product, 6(02), 194–200. https://doi.org/10.35473/ijpnp.v6i02.2508
Hidayatullah, M., Rakhmatullah, A. N., & Perdana, D. (2023). Penetapan Kadar Fenolik Total Dan Flavonoid Total Ekstrak Etanol Batang Bajakah Tampala ( Spatholobus littoralis Hassk .). 6(2), 41–52.
Lia Agustin, & Rilla Agustina. (2021). Komparasi Unjuk Kerja Peralatan Spektrofotometer Uv-Vis Perkin Elmer Lambda 3 Dengan Hitachi U-2900 Pada Penentuan Total Phenolic Content. Jurnal Teknik Ilmu Dan Aplikasi, 9(1), 42–45. https://doi.org/10.33795/jtia.v9i1.9
Makin, F. M. P. R., Welsiliana, W., & Wiguna, G. A. (2022). Karakterisasi Stomata dan Trikomata Daun Kirinyuh (Chromolaena odorata L.). Journal Science of Biodiversity, 3(1), 61–67. https://doi.org/10.32938/jsb/vol3i1pp61-67
Marsella, V. R., & Saleh, C. (2024). Review Artikel : Identifikasi Fitokimia , Potensi Aktivitas Antioksidan Dan Toksisitas Daun Balakacida ( Chromolaena Odorata L .) Article Review : Phytochemical Identification , Potential Antioxidant Activity And Toxicity Of Balakacida Leaves ( Chromolaen. 239–242.
Mumarli, M., Mulqie, L., Farmasi, P., Matematika, F., Alam, P., & Bandung, U. I. (2024). Penapisan Fitokimia Ekstrak Etanol Daun Kirinyuh Hasil Metode Ekstraksi Refluks dan Maserasi. 107–114.
Ramayani, S. L., Permatasari, E. A., Novitasari, I., & Maryana, M. (2021). Pengaruh Metode Ekstraksi Terhadap Kadar Total Fenolik, Kadar Total Flavonoid Dan Aktivitas Antioksidan Ekstrak Daun Mengkudu (Morinda citrifolia L.). Jurnal Ilmu Farmasi Dan Farmasi Klinik, 18(01), 40. https://doi.org/10.31942/jiffk.v18i01.4898
Safitri, E. I., Anggraeni, S., Utomo, A. N., & Hidayati, D. N. (2023). Perbandingan Kadar Flavonoid Dan Fenolik Ekstrak Etanol Kulit Dan Biji Mangga (Mangifera Indica L.) Varietas Arummanis Dan Manalagi. Medfarm: Jurnal Farmasi Dan Kesehatan, 12(1), 19–29. https://doi.org/10.48191/medfarm.v12i1.172
Sari, W. E., Darmawi, D., Wianda, M., Erina, E., Zamzami, R. S., Hambal, M., Salim, M. N., Hennivanda, H., & Lubis, T. M. (2021). 5. Antimicrobial Activity of Balakacida (Chromolaena odorata) Endophytic Bacteria Isolated from Aceh Besar Against Staphylococcus aureus and Pseudomonas aeruginosa. Jurnal Medika Veterinaria, 14(2), 125–131. https://doi.org/10.21157/j.med.vet..v14i2.19415
Suhanda, H. (2022). Troubleshooting Dalam Analisis Spektrofometer UV-VIS. In Sustainability (Switzerland). http://scioteca.caf.com/bitstream/handle/123456789/1091/RED2017-Eng-8ene.pdf?sequence=12&isAllowed=y%0Ahttp://dx.doi.org/10.1016/j.regsciurbeco.2008.06.005%0Ahttps://www.researchgate.net/publication/305320484_Sistem_Pembetungan_Terpusat_Strategi_Melestari
Sukma, M., Nurlansi, & Nasrudin. (2022). Total Fenolik dan Aktivitas Antioksidan Seduhan Kulit Batang Soni (Dillenia serrata Thunb). Jurnal Ilmu Kimia Dan Pendidikan Kimia, 11, 27–34. http://ojs.uho.ac.id/index.php/SAINSe-mail:
Susanti, S., Sundari, R. S., Rizkuloh, L. R., & Mardianingrum, R. (2021). Pengaruh Perbedaan Pelarut Terhadap Kadar Fenol Total Dan Aktivitas Antioksidan Ekstrak Gadung (Dioscorea hispida Dennst.). Biopropal Industri, 12(1), 43. https://doi.org/10.36974/jbi.v12i1.6482
Tommy, M., Putra Pratama, N., & Purnomo Sari, K. R. (2022). Perbandingan Kadar Total Fenolik dan Flavonoid Ekstrak Etanol Daun, batang, dan Akar Kirinyuh (Chromolaena odorata L.) dengan Menggunakan Metode Spektrofotometri UV-Vis. Jurnal Pharmacia Mandala Waluya, 1(5), 217–231. https://doi.org/10.54883/jpmw.v1i5.48
Tulnisa, H., Ahmad, A. R., & Hasnaeni, H. (2025). Optimasi Metode Ekstraksi Maserasi Dan Ultrasound Assisted Extraction (Uae) Rimpang Kunyit (Curcuma Longa L.) Dan Aktivitas Antioksidannya. Jurnal Review Pendidikan Dan Pengajaran, 8(2), 4101–4111. https://doi.org/10.31004/jrpp.v8i2.44563
Utami, Y. P., Mubarak, F., & Rahman, N. F. (2023). Variasi Teknik Pengeringan Daun Tekelan (Chromolaena odorata L.) Mempengaruhi Aktivitas Antioksidan: Penelitian Laboratorium dengan Metode ABTS. Health Information : Jurnal Penelitian, 15(2), 180–189. https://doi.org/10.36990/hijp.v15i2.775
Wardani, M. R., Hairani, R., Studi, P., Kimia, S., Matematika, F., Alam, P., & Mulawarman, U. (2024). Uji Fitokimia Dan Uji Toksisitas Ekstrak Etanol Batang Bajakah (Uncaria Cordata (Lou .) Merr.) Terhadap Artemia Salina L . Phytochemical Test And Toxicity Test Of Bajakah Stem ( Uncaria cordata ( Lour .) Merr .) Ethanol Extract On Artemia salina L . 63–68.
Ziadaturrif’ah, D., Darmanti, S., & Budihastuti, R. (2019). Buletin Anatomi dan Fisiologi Volume 4 Nomor 2 Agustus 2019 Potensi Autoalelopati Ekstrak Daun Kirinyuh (Chromolaena odorata L.) The Potential Allelophatic of Leaf Kirinyuh Extract. 4.
DOI: https://doi.org/10.14421/biomedich.2026.151.239-244
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