Molecular Identification and Characterization of Lipase-Producing Bacteria Isolated from Wawolesea Hot Spring, Southeast Sulawesi

Sapto Raharjo, Muhammad Azwar Syah, Andi Tantri, Tien Tien, Lina Lestari

Abstract


The Wawolesea hot spring in Southeast Sulawesi represents an extreme ecosystem characterized by high temperature and salinity, providing a potential habitat for thermo-halophilic bacteria that produce industrial enzymes, particularly lipase. Here, we carried out molecular identification of lipase-producing bacteria isolated from the Wawolesea hot spring using 16S rRNA gene analysis. The sequence comparison showed that isolate K.111 was the most closely related to Bacillus thuringiensis. This supports the hypothesis that the Wawolesea hot spring harbors thermo-halophilic bacterial communities with potential applications in industrial biocatalysis.


Keywords


lipase; thermo-halophilic bacteria; Wawolesea

Full Text:

PDF

References


Andini, L., & Afifatul, N. (2025). Phylogenetic analysis of thermophilic bacterial isolates based on 16S rRNA gene sequences. Jurnal Bioteknologi dan Biosains Indonesia, 12, 33.

Arslan, M., Tezcan, E., Camci, H., & Avci, M. K. (2021). Effect of DNA concentration on band intensity and resolution in agarose gel electrophoresis. Van Sa?l?k Bilimleri Dergisi, 14(3), 326. https://doi.org/10.52976/vansaglik.969547

Aulia, F., Jaya, M., & Ningsih, D. (2022). Diversity of thermophilic bacteria in Indonesian hot springs. Jurnal Mikrobiologi Indonesia, 17, 145.

Buchori, D., Rahmadani, R., & Suryana, H. (2023). Visualisasi DNA genom hasil isolasi bakteri lipolitik. Jurnal Mikrobiologi Indonesia, 8, 101.

Corneles, R., Sutanto, S., & Wijaya, I. (2023). Biochemical and structural properties of thermostable lipase-producing Bacillus spp. isolated from hot springs. Journal of Applied Microbiology, 134, lxad062. https://doi.org/10.1093/jambio/lxad062

Eling, K. S. D., Kurniawan, R., & Muhimmah, I. (2014). Karakteristik primer pada polymerase chain reaction (PCR) untuk sekuensing DNA: Mini review. Seminar Informatika Medis, 93. http://snimed.fit.uii.ac.id/

Firda, A., Rahman, A., & Nurfadila, D. (2025). Genetic distance analysis among Bacillus species based on 16S rRNA gene. Jurnal Bioteknologi dan Biosains Indonesia, 12, 22.

Friliana, R., Rindyaneputri, M., et al. (2025). Phylogenetic relationship of Bacillus isolates from geothermal springs using BLAST analysis. Jurnal Bioteknologi dan Biosains Indonesia, 12, 88.

Gupta, R., Gupta, N., & Rathi, P. (2004). Bacterial lipases: An overview of production, purification and biochemical properties. Applied Microbiology and Biotechnology, 64, 763. https://doi.org/10.1007/s00253-004-1568-8

Gurumurthy, D. M., et al. (2020). Thermophilic bacteria and thermostable enzymes in industrial biotechnology: Challenges and opportunities. Biotechnology Advances, 43, 107581. https://doi.org/10.1016/j.biotechadv.2020.107581

Hasan, F., Shah, A. A., & Hameed, A. (2006). Industrial applications of microbial lipases. Enzyme and Microbial Technology, 39, 235. https://doi.org/10.1016/j.enzmictec.2005.10.016

Ifandi, D., & Alwi, M. (2018). Indonesia’s geothermal potential: Review of energy sustainability and microbiological perspectives. Jurnal Energi Indonesia, 4, 33.

Jaeger, K. E., & Eggert, T. (2002). Lipases for biotechnology. Current Opinion in Biotechnology, 13, 390. https://doi.org/10.1016/S0958-1669(02)00341-5

Jamaluddin, M., et al. (2018). Isolation and screening of thermophilic lipase-producing bacteria from Indonesian geothermal areas. Indonesian Journal of Biotechnology, 23, 45. https://doi.org/10.22146/ijbiotech.34100

Khalid, H., & Abdulrahman, A. (2024). Molecular identification and characterization of lipolytic Bacillus species from geothermal environments. Journal of Genetic Engineering and Biotechnology, 22, 45. https://doi.org/10.1016/j.jgeb.2023.10.007

Khairunnisah, Bagis, F. A. Z., Andriani, F., Anwar, K., Gifari, Z. A. L., Rosyidi, A., & Ali, M. (2022). Isolasi, identifikasi, dan karakterisasi Pediococcus spp. dan Lactobacillus spp. dari saluran pencernaan entok (Cairina moschata) sebagai kandidat probiotik unggas. Jurnal Ilmiah Indonesia, 7, 18136. http://dx.doi.org/10.36418/syntax-literate.v7i12.10835

Lee, S. Y., Lim, J. M., & Park, E. H. (2022). Characterization of thermostable lipase from thermophilic Bacillus sp. for industrial applications. Frontiers in Microbiology, 13, 987654. https://doi.org/10.3389/fmicb.2022.987654

Mahadi, I., Sayuti, I., & Nursal, N. (2024). Ekstraksi DNA Nibung (Oncosperma tigillarium (Jack) Ridl.) menggunakan beberapa jenis buffer terhadap kualitas genomik DNA. Jurnal Biologi Papua, 16(2), 129. https://doi.org/10.31957/jbp.3780

Muhamad, A. S., Sulfatimah, Y., Yaddi, Y., Isnaini Ulfa, N., & Elviantari, A. (2024). Identifikasi molekuler bakteri lipolitik yang diisolasi dari sedimen mangrove Teluk Kendari. Jurnal Penelitian Biologi, 11(1), 68.

Muharni, M., & Anggraini, D. (2015). Molecular identification of lipolytic bacteria isolated from geothermal springs in West Sumatra. Indonesian Journal of Biotechnology, 20, 22. https://doi.org/10.22146/ijbiotech.12345

Muzuni, M., Jamaluddin, J., Suriana, S., Ardiansyah, A., & Yanti, N. A. (2024). Skrining bakteri termohalofilik penghasil L-asparaginase dari sumber air panas Wawolesea Sulawesi Tenggara dan uji aktivitas enzimnya. Alchemy: Jurnal Penelitian Kimia, 20, 12.

Nafian, M., et al. (2022). Mapping geothermal microbial diversity in Indonesia: An overview. Jurnal Ilmu Alam Indonesia, 9, 210.

Norashirene, M. N., Baharum, S. N., Sabri, S., et al. (2013). Thermostable lipases from Bacillus and Geobacillus species: Biochemical characterization and industrial perspectives. Enzyme and Microbial Technology, 52, 297. https://doi.org/10.1016/j.enzmictec.2013.02.004

Panda, A. K., Mishra, S., Mishra, P. K., & Thatoi, H. (2018). Thermostable microbial lipases: Sources, properties and applications. Biotechnology Reports, 20, e00268. https://doi.org/10.1016/j.btre.2018.e00268

Parista, N., Kurnia, E., & Hasanah, N. (2024). Analisis kualitas pita DNA hasil isolasi genom bakteri. Jurnal Bioteknologi dan Biosains Indonesia, 11, 155.

Sahadeva, M. L., & Pertiwi, N. P. D. (2024). Konstruksi pohon filogenetik spesies dalam famili Orchidaceae berdasarkan marka gen matK kloroplas: Studi in silico. Wahana Matematika dan Sains: Jurnal Matematika, Sains, dan Pembelajarannya, 17(3), 12. https://doi.org/10.23887/wms.v17i3.87986

Saleky, D., & Amir, N. (2023). Reliability of phylogenetic tree reconstruction based on 16S rRNA gene sequences. Jurnal Bioteknologi dan Biosains Indonesia, 10, 70.

Sendo, E., Rahmawati, D., & Lestari, A. (2022). Purification and biochemical characterization of thermostable lipase from thermophilic bacteria isolated from hot springs. Enzyme and Microbial Technology, 161, 110098. https://doi.org/10.1016/j.enzmictec.2022.110098

Setyawati, D., & Zubaidah, A. (2021). Pengaruh suhu anil terhadap hasil PCR amplifikasi gen bakteri. Jurnal Bioteknologi dan Biosains Indonesia, 8, 123.

Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Production, purification, characterization, and applications of lipases. Biotechnology Advances, 19, 627. https://doi.org/10.1016/S0734-9750(01)00086-6

Singh, A. K., & Kumar, R. (2019). Microbial lipases: A review of biotechnological applications and molecular approaches. Critical Reviews in Biotechnology, 39, 545. https://doi.org/10.1080/07388551.2019.1576021

Vertiana, R., Rasyid, M., et al. (2023). Bootstrap reliability in phylogenetic analysis of Bacillus isolates. Jurnal Bioteknologi dan Biosains Indonesia, 11, 60.




DOI: https://doi.org/10.14421/biomedich.2026.151.215-220

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2026 Sapto Raharjo, Muhammad Azwar Syah, Andi Tantri, Tien, Lina Lestari



Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

CC BY NC
This work is licensed under a CC BY-NC