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Bioactivities of Prosopis africana Whole Fruit: Antibacterial and Urease Inhibition Properties
Abstract
Prosopis africana (Guill. & Perr.) Taub. (Fabaceae) is a West African tree traditionally used for treating infections. However, a systematic evaluation of the bioactivities of its fruit remains limited, and studying the fruit offers a more sustainable and conservation-friendly approach compared to harvesting the bark or roots. This study aimed to investigate the antibacterial and urease inhibitory properties of the whole fruit extract of P. africana and its solvent-partitioned fractions. The powdered whole fruit was macerated in 70% methanol to obtain a crude extract (PAF-0). A 1.1 kg portion of PAF-0 was subsequently partitioned using 1 L volumes per cycle into n-hexane (PAF-1), dichloromethane (PAF-2), ethyl acetate (PAF-3), and aqueous (PAF-4) fractions. Antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria using the Microplate Alamar Blue Assay (MABA). Jack bean urease inhibition was assessed spectrophotometrically. All experiments included vehicle controls (DMSO) and were performed with three independent replicates (n=3). The dichloromethane fraction (PAF-2) exhibited the strongest antibacterial activity, showing 84% and 88% inhibition against Escherichia coli and Salmonella typhi, respectively, which was comparable to the standard drug ofloxacin. In the urease inhibition assay, the n-hexane fraction (PAF-1) and the crude extract (PAF-0) were the most potent, with IC50 values of 27.1 µg/mL and 27.6 µg/mL, respectively. The findings indicate that the dichloromethane and n-hexane fractions of P. africana fruit possess significant antibacterial and urease inhibitory activities. This provides a scientific basis for its traditional uses against infections and highlights its potential as a rich source of bioactive compounds for further pharmacological development.
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Adamu, H. M., Ibrahim, J. S., Amin, B. D. E., Mahmoud, A. A., & Idris, A. H. (2024). Exploring the phytochemical properties of Prosopis africana plants and assessing their antimicrobial potential against selected clinical strains. Path of Science, 10(6), 1001–1008. https://pathofscience.org/index.php/ps/article/view/3144
Akhlaq, M., Rizwani, G. H., Sultana, S., Dilshad, R., Ahmad, M., Nisar, R., Afzal, A., Zainab, R., Ozdemir, F. A., & Solowski, G. (2025). Urease enzyme: Structure, function, and its role in Helicobacter pylori pathogenesis and treatment strategies. Annual Methodological Archive Research Review, 3(8), 1088–1111. https://amresearchreview.com/index.php/Journal/article/view/684
Al-Rooqi, M. M., Mughal, E. U., Raja, Q. A., Hussein, E. M., Naeem, N., Sadiq, A., Asghar, B. H., Moussa, Z., & Ahmed, S. A. (2023). Flavonoids and related privileged scaffolds as potential urease inhibitors: A review. RSC Advances, 13(5), 3210–3233. https://pubs.rsc.org/en/content/articlelanding/2023/ra/d2ra08284e
Alimata, B., Seydou, S., Souleymane, C., Eli, C., Esther, B.-K. L. M., Vincent, O., Ablassé, R., Noufou, O., & Martin, K. (2021). Therapeutic properties of aqueous extracts of leaves and stems bark of Prosopis africana (Guill. & Perr.) Taub. (Fabaceae) used in the management of dental caries. Journal of Drug Delivery and Therapeutics, 11(6), 108–114. https://jddtonline.info/index.php/jddt/article/view/5152
Almarmouri, C., El-Gamal, M. I., Haider, M., Hamad, M., Qumar, S., Sebastian, M., Ghemrawi, R., Muhammad, J. S., Burucoa, C., & Khoder, G. (2025). Anti-urease therapy: A targeted approach to mitigating antibiotic resistance in Helicobacter pylori while preserving the gut microflora. Gut Pathogens, 17(1), 37. https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-025-00636-y
Chopra, B., & Dhingra, A. K. (2021). Natural products: A lead for drug discovery and development. Phytotherapy Research, 35(9), 4660–4702. https://doi.org/10.1002/ptr.7099
Dean, M. (2024). Exploring ethnobotanical knowledge: Qualitative insights into the therapeutic potential of medicinal plants. Golden Ratio of Data in Summary, 4(2), 154–166. https://doi.org/10.52970/grdis.v4i2.491
Doughari, J. H., & Saa-Aondo, M. (2021). Phytochemical analysis of crude methanol extracts and antimicrobial activity of n-hexane fractions of methanol seed and pod extracts of Prosopis africana on some selected microorganisms. Archives, 2, 121–137. https://pharmacologyonline.silae.it/files/archives/2021/vol2/PhOL_2021_2_A016_Doughari.pdf
Kebede, T., Gadisa, E., & Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PLOS ONE, 16(3), e0249253. https://doi.org/10.1371/journal.pone.0249253
Lu, Q., Tan, D., Xu, Y., Liu, M., He, Y., & Li, C. (2021). Inactivation of jack bean urease by nitidine chloride from Zanthoxylum nitidum: Elucidation of inhibitory efficacy, kinetics and mechanism. Journal of Agricultural and Food Chemistry, 69(46), 13772–13779. https://doi.org/10.1021/acs.jafc.1c05609
Malongweni, S. O., Mrubata, K., van Tol, J., Abd Elbasit, M. A., & Harebottle, D. M. (2025). Consequences of invasive Prosopis (mesquite) on vegetation, soil health, biodiversity, and compliance of management practices in South African rangelands: A review. Grasses, 4(1), 2. https://doi.org/10.3390/grasses4010002
Nava-Solis, U., Rodriguez-Canales, M., Hernandez-Hernandez, A. B., Velasco-Melgoza, D. A., Moreno-Guzman, B. P., Rodriguez-Monroy, M. A., & Canales-Martinez, M. M. (2022). Antimicrobial activity of the methanolic leaf extract of Prosopis laevigata. Scientific Reports, 12(1), 20807. https://doi.org/10.1038/s41598-022-25455-0
Oliyaei, N., Hashempur, M. H., Zomorodian, K., Tanideh, N., Noori, M., Abbasi, A., Mahdavi, M., & Iraji, A. (2024). An overview of potential algae-derived bioactive compounds against urease-positive microorganisms. Algal Research, 77, 103372. https://doi.org/10.1016/j.algal.2024.103372
Pikhtirova, A., Pecka-Kie?b, E., & Zigo, F. (2023). Antimicrobial activity of saponin-containing plants: Review. Journal of Dairy, Veterinary & Animal Research, 2(4), 121–127. https://medcraveonline.com/JDVAR/JDVAR-12-00336.pdf
Salam, M. A., Al-Amin, M. Y., Salam, M. T., Pawar, J. S., Akhter, N., Rabaan, A. A., & Alqumber, M. A. (2023). Antimicrobial resistance: A growing serious threat for global public health. Healthcare, 11(13), 1946. https://doi.org/10.3390/healthcare11131946
Salawu, K. M., Wang, Y., Maharjan, R., & Ajaiyeoba, E. O. (2020). Antibacterial screening and isolation of compounds from Detarium microcarpum stem bark against methicillin resistant Staphylococcus aureus. Journal of Science and Practice in Pharmacy, 7(1), 400–405. https://jsppharm.org/home/abstract.php?id=82
Singh, K., Gupta, J. K., Chanchal, D. K., Shinde, M. G., Kumar, S., Jain, D., Almarhoon, Z. M., Alshahrani, A. M., Calina, D., & Sharifi-Rad, J. (2025). Natural products as drug leads: Exploring their potential in drug discovery and development. Naunyn-Schmiedeberg's Archives of Pharmacology, 398(5), 4673–4687. https://doi.org/10.1007/s00210-025-03899-5
Zhong, J., Lu, P., Wu, H., Liu, Z., Sharifi-Rad, J., Setzer, W. N., & Suleria, H. A. (2022). Current insights into phytochemistry, nutritional, and pharmacological properties of Prosopis plants. Evidence-Based Complementary and Alternative Medicine, 2022, 2218029. https://doi.org/10.1155/2022/2218029
Zhou, G., Wang, Q., Wang, Y., Wen, X., Peng, H., Peng, R., Shi, Q., Xie, X., & Li, L. (2023). Outer membrane porins contribute to antimicrobial resistance in Gram-negative bacteria. Microorganisms, 11(7), 1690. https://doi.org/10.3390/microorganisms11071690
DOI: https://doi.org/10.14421/biomedich.2025.142.1547-1550
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Copyright (c) 2026 Kayode Muritala Salawu, Saad Toyin Abdullahi, Akeem Ganiyu Rabiu, Olutosin Alarape Adegboye, Muhammad Aamer, Nuzhat Shehla, Yan Wang
Biology, Medicine, & Natural Product Chemistry |
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