Antioxidant Activities of the Leaf Extract and Fractions of Dryopteris filix-mas (L.) Schott could be Attributed to The Abundance of Polyphenol Compounds

Earnest Oghenesuvwe Erhirhie, Emmanuel Emeka Ilodigwe, Daniel Lotanna Ajaghaku, Blessing Ogechukwu Umeokoli, Peter Maduabuchi Eze, Festus Basden Chiedu Okoye


Dryopteris filix mas (D filix-mas) is wildly used in ethnomedicine for the management of rheumatoid arthritis, wounds and other diseases. We investigated the anti-oxidant activities of its leaf extract, and chromatographic fractions. The ethanol leaf extract was partitioned into four fractions; n-hexane, ethyl acetate, n-butanol and water. Ferric reducing anti-oxidant power (FRAP), 1, 1-diphenyl-2-picrylhydrazil (DPPH) and nitric oxide (NO) scavenging in vitro assays were carried out on the extract and fractions at 6.25, 12.5, 25, 50, 100, 200, 400 and 800 µg/mL. The most active fraction (ethyl acetate fraction) was further purified using chromatographic techniques to isolate its major compound whose structure was elucidated using ID nuclear magnetic resonance (NMR) and mass spectrometry. The ethyl acetate fraction produced the highest free radical scavenging activity among the other fractions. The fraction (VLC-E7) from which the bioactive compound, quercetin-3-O-αL-rhamnopyranoside, was isolated had the best FRAP and DPPH scavenging activities with EC50 and IC50 values of 88.81 ± 3.41 and 26.87 ± 0.24 respectively more than the ethyl acetate fraction. This study revealed that the polyphenol flavonoid, quercetin-3-O-αL-rhamnopyranoside could be responsible for antioxidant activity of ethno-medicinal property of D filix-mas leaf.


Dryopteris filix-mas; wounds; rheumatoid arthritis; quercetin-3-O-αL-rhamnopyranoside; anti-oxidant properties

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Ahlem, R., Souad, I.B., Béatrice, B., Valérie, M.L., Fathi, M., Evelyne, O., Jamila, K.C and Malika, T.A., 2015, Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae), JAPS, 5 (01): 052-057.

Ajaghaku, D.L., Obasi, O., Umeokoli, B.O., Ogbuatu, P., Nworu, C.S., Ilodigwe, E.E., and Okoye, F.B.C., 2017, In vitro and in vivo antioxidant potentials of Alchornea floribunda leaf extract, fractions and isolated bioactive compounds, Avicenna J Phytomed, 7 (1): 80-92.

Bafor, E.E., Omokaro, W.O., Uwumarongie, O.H., Elvis-Offiah, U.B., Omoruyi, O, Viegelmann, C.V. and Edrada-Ebel, R., 2017. Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity. Journal of Medicinal Plants for Economic Development, 1(1):1-12.

Deepa, M. and Renuka, D., 2014, Potent anti-inflammatory medical plant-a review, Int J Pharm Pharm Sci., 6 (4):43-49.

Erhirhie EO, Emeghebo CN, Ilodigwe EE, Ajaghaku DL, Umeokoli BO, Eze PM, Ngwoke KG, Okoye FBC. Dryopteris filix-mas (L.) Schott ethanolic leaf extract and fractions exhibited profound anti-inflammatory activity. Avicenna J Phytomed, 2019; 9(4): 396-409.

Ezeja, M.I., Yusuf, N., Omeh, S., Onoja, O. and Ijeoma, H.U., 2015, Antiinflammatory and Antioxidant Activities of the Methanolic Leaf Extract of Cissus aralioides, Am J Pharmacol Sci, 3 (1): 1 -6.

Habibur, R., Manjula, K., Anoosha, T., Nagaveni, K., Chinna, M.E., and Dipankar, B., 2013, In-vitro antioxidant activity of Citrulus lanatus seed extracts, Asian Journal of Pharm. Clin. Res. 6 (3): 152-157.

Hussain T.,Tan, B., Yin, Y., Blachier, F., Tossou, M.C.B., Rahu N., 2016. Oxidative Stress and Inflammation: What Polyphenols Can Do for Us. Hindawi Publishing Corporation

Oxidative Medicine and Cellular Longevity Volume 2016, Article ID 7432797, 9 pages

Khatoon, M., Islam, E, Islam, R., Rahman, A.A., Alam, A.H.M.K., Khondkar, P., Rashid, M. and Parvin, S., 2013, Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves, BMC Res Notes. 6:121. 1-7.

Kumar, S., Sandhir, R. and Ojh, S., 2014, Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves, BMC Res Notes. 7:560.1-7.

Li J., Liu X., Shen L., Zeng W., Qiu G., 2016, Natural plant polyphenols for alleviating oxidative damage in man: Current status and future perspectives Tropical Journal of Pharmaceutical Research; 15 (5): 1089-1098.

Maalik, A., Farhan, A.K., Amara, M., Adeem, M., Saira, A., Muhammad, A., Sabiha, K., Yasir, A. and Imran, T., 2014, Pharmacological Applications of Quercetin and its Derivatives: A Short Review, Trop J Pharm Res, 13 (9): 1561-1566.

Mandal, A. and Mondal, A.K., 2014, Studies on antimicrobial activities of some selected ferns and lycophytes in Eastern India with special emphasis on ethno-medicinal uses, Afr. J. Plant Sci, 5(7):412-420.

Mir, S.A, Jan, Z., Mir, S., Dar, A.M. and Chitale, G., 2017, A Concise Review on Biological Activity of Tridax procumbens Linn, Organic Chem Curr Res, 6: 177.

Monika, M., Michae S., Maegorzta P. and Hanna, C., 2011, Evaluation of antioxidant potential of flavonoids: An in vitro study, Acta Pol Pharm, 68 (4):611-615.

Nimse, S.B., Pal, D., 2015. Free radicals, natural antioxidants, and their reaction mechanisms. Royal Society of Chemistry, 5, 27986-28006. doi: 10.1039/C4RA13315C.

Ojo, O.A., Oloyede, O.I., Olarewaju, O.I., Ojo, A.B., Ajiboye, B.O. and Onikanni, S.A., 2013, Toxicity Studies of the Crude Aqueous Leaves Extracts of Ocimum gratissimum in Albino Rats, IOSR-JESTFT, 6 (4):34-39.

Patel, A., Patel, A., Patel, N.M., 2010, Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea Linn.leaves (Leguminosae), Pharmacogn Res. 2:152–8.

Sekendar, A.M., Mostafa, K., Raihan, M.O., Rahman, M.K., Hossain, M.A. and Alam, M.S., 2012, Antioxidant and Cytotoxic activities of Methanolic extract of Dryopteris filix-mas (L.) Schott Leaves, Int. j. drug dev. res, 4(2): 223-229.

Tagarelli, G., Tagarelli, A. and Piro, A., 2010, Folk medicine used to heal malaria in Calabria (southern Italy), J Ethnobiol Ethnomed, 6 (27): 1-16.

Urban, J., Tauchen, J., Langrova, I. and Kokoska, L., 2014, In vitro motility inhibition effect of Czech medicinal plant extracts on Chabertia ovina adults, J. Anim. Plant Sci, 21(2): 3293-3302.

Uwumarongie, H.O., Enike, M.A. and Bafor, E.E., 2016, Pharmacognostic evaluation and gastrointestinal activity of Dryopteris filix-mas (L.) schott (Dryopteridaceae), Ew J Herbal ChemPharmacol Res, 2(1): 19 – 25.

Valentyna, M., Iryna, T., Tetyana, D. and Larysa, M., 2017, A review of the medicinal ferns of Ukraine, Scr. Sci. Pharm, 4 (1): 46-62.

Yan, H., Yuan, Y., Xi, Z., Kun, Z., Shaohua, C. and Zhiyun, D., 2015. Curcumin, Inflammation, and Chronic Diseases: How Are They Linked?, Molecules, 20, 9183-9213; doi:10.3390/molecules20059183.

Zhang, Y., Wang, D., Yang, L., Zhou, D., Zhang, J. 2014, Purification and Characterization of Flavonoids from the Leaves of Zanthoxylum bungeanum and Correlation between Their Structure and Antioxidant Activity, PLoS ONE, 9(8): e105725. doi:10.1371/journal.pone.010572.



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Copyright (c) 2020 Earnest Oghenesuvwe Erhirhie, Emmanuel Emeka Ilodigwe, Daniel Lotanna Ajaghaku, Blessing Ogechukwu Umeokoli, Peter Maduabuchi Eze, Festus Basden Chiedu Okoye

Biology, Medicine, & Natural Product Chemistry

ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Sunan Kalijaga State Islamic University &
Society for Indonesian Biodiversity

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