Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

Abstract

Background: Vinca rosea is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of Vinca rosea was selected to investigation its larvicidal activity against the 3^rd instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) Aedes aegypti.  Five concentrations of Vinca fruit extract were tested against the 3^rd instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC₅₀ anf LC₉₀) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC₅₀ and LC₉₀ values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.