Analysis of Bull Sperm DNA Abnormalities Due to Cadmium Accumulation

Fuad Fitriawan

Abstract


Sperm abnormalities can occur by various causes. Abnormality of sperm is usually characterized by abnormal sperm motility and viability. This was caused by the inability of mitochondria on ATP-ase in producing ATP and ecto-enzyme Cik role in keeping the movement so that movement of sperm motility declines. Research that leads to total abnormal sperm DNA analysis is still rare. The purpose of this research was to get the results of the molecular characteristic picture of the overall characteristics of DNA loci that have abnormalities in bull sperm and get a picture of differences in overall DNA loci of abnormal and normal sperm. This research was conducted in October-December 2016. With the results of Group I consists of D, E with a percent similarity of 92.308%, group II consists of C and Group I with a percentage similarity of 50.125%, group II consists of A and B with a percent similarity of 100%, group II and group III with a percentage similarity of 0%. Based on the above data it can be concluded that the treatment A and B is not suspected to cause DNA damage compared to treatment C, D and E.

Keywords


Abnormality; Spermatozoa; Cow; PCR-RAPD; cadmium accumulation

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References


Agritubella, M. S. 2009. Infertilitas. Artikel kesehatan reproduksi http://situs.kesrepro.info/kb/referensi2.htm. Retrieved at 29 March 2010.

Coffee, C. J. 1998. Metabolism. 1st ed. Fence Creek Publising. Madison: xiii + 454 p.

Curry MR, Watson PF. 1995. Sperm Structure and Function in: Graud zinkas JG, Yovich JL, (eds). Gametes-The Spermatozoon. Great Britain: Cambridge University Press. Pages 45-67.

Curry, M. R. & Watson, P. F. 1995. Sperm Structure and Function. Great Britain. Cambridge University Press. Pages 45-67.

Destiany. M. 2007. Pengaruh Pemberian merkuri Klorida terhadap Struktur Mikroanatomi hati Ikan Mas. Thesis. Department of Biology Universitas Negeri Semarang

Fitriawan, F. 2006. Kemampuan Anti MPS dalam Menghambat Motilitas dan Viabilitas Spermatozoa Kambing, Domba, dan Sapi. Thesis Department of Biology Faculty of Science and Technology UIN Malang.

Ginzburg, SA, 1972. Fertilization in fishes and the problem of polyspermy. Wiener Bindery. Jerussalem.

Guven, M. C., Can, B., Saran, Y. 1998. Ultrastructural Changes in the Spermatozoa of Infertil Man. Journals from dept. histology-embriology, medical School of Ankara University Turkey.

Guyton AC, Hall JE. 1997. Buku Ajar Fisiologi Kedokteran. Diterjemahkan oleh Irwati S, LMA Ken Ariata T, Alek S. Jakarta: Penerbit Buku Kedokteran EGC. Pages: 309-314.

Hafez, E.S.E. 1977. Techniques for Improving Reproduction Efficiency, pp.455-479. The CV Mosby Company: St. Louis.

Mason, CF. 1981. Biology of Freshwater Pollution.Longman lnc, New York.

Moleong, L. J. 1997. Metodologi Penelitian Kualitatif. Bandung, P.T. Remaja Rosda Karya.

Muchtaromah, B. 1999. Pengaruh Pemberian Metoklopramid terhadap gambaran Histologis Testis Tikus Putih (Rattus norwegicus). Thesis Postgraduate Program Department of Bioreproduksi UNAIR Surabaya.

Nelson, RW. 2003. Morphology of Normal and Abnormal Canine Spermatozoa. Couto CG. Journal of Small Animal Internal medicine. Mosby

Partodiharjo, Soebadi, 1992. Ilmu Reproduksi Hewan. Mutiara Sumber Widya. Jakarta Pusat. Cetakan ke III Pages 523-531.

Rijnders PM. 1996. The Spermatozoon-Theory. In: Brass JW, et al. Editors. IVF lab-laboratory as pects of in vitro vertilization. NV Organon. Pages 23-39.

Salisbury, G. W. 1985. Inseminasi Buatan Pada Sapi. Gadjah Mada University Press Yogyakarta.

Sarkar S, Hanry JB. 1996. Andrology Laboratory and fertility Assessment, in: Henry JB, Editors. Clinical Diagnosis and Management by Laboratory Metods. Philadelphia.

Setyono, P., Soetarto, E. S., 2008. Biomonitoring Degradasi Ekosistem Akibat Limbah CPO Muara Sungai Mentaya Kalimantan Tengah dengan Metode Elektromorf Isozim Esterase. Jurnal Biodiversitas Vol 9. Nomor 3. Universitas Sebelas Maret Surakarta. Pages 232-236.

Sutarno, 2008. Diktat Materi Bioteknologi dan Seluler. Faculty of Mathematics and Natural Sciences Universitas Sebelas Maret Surakarta. Pages. 35-40.

Susilowati, T. 2005. Fisiologi Spermatozoa: Kapasitasi, reaksi Akrosom dan Fertilisasi. Faculty of Animal Husbandry Universitas Brawijaya Malang.

Toelihere. 1985. Fisiologi Reproduksi pada Ternak. Publisher Angkasa Bandung. Pages 93 – 115.

Toelihere. 1993. Analisis Kualitas Semen pada Ternak. Publisher Angkasa Bandung.




DOI: https://doi.org/10.14421/biomedich.2017.61.5-8

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Biology, Medicine, & Natural Product Chemistry

ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Sunan Kalijaga State Islamic University &
Society for Indonesian Biodiversity

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