Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton

Muhammad Ja’far Luthfi


Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.


Skeleton; Alizarin Red S; Alcian Blue; Reptilia; Double-staining

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Copyright (c) 2016 Muhammad Ja’far Luthfi

Biology, Medicine, & Natural Product Chemistry
ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Published by Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity.

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This work is licensed under a CC BY-NC