2024-03-28T08:37:41Z
https://sciencebiology.org/index.php/BIOMEDICH/oai
oai:ojs.sciencebiology.org:article/36
2018-08-20T01:52:01Z
BIOMEDICH:ART
Starch-Glycerol Based Edible Film and Effect of Rosella (Hibiscus Sabdariffa Linn) Extract and Surimi Dumbo Catfish (Clarias gariepinus) Addition on Its Mechanical Properties
Sedyadi, Endaruji
Aini, Syafiana Khusna
Anggraini, Dewi
Ekawati, Dian Prihatiningtias
Edible film; Rosella Extract; Surimi Dumbo catfish; Mechanical Properties
Effect of Rosella (Hibiscus Sabdariffa Linn) Extract and Surimi Dumbo catfish (Clarias gariepinus) addition on Starch-Based Edible Film-Glycerol Mechanical Properties has been done. The purpose of this study is to create an active environment-friendly packaging material. Surimi additions are intended to improve the mechanical properties of bioplastics and additions of Rosella extract intended as a bio-indicator of acidity. The method used was Solvent Casting. An amount of surimi and rosella extract varied to obtain the best mechanical properties. The results shows that the addition of surimi and rosella flower extract significantly effect the elongation of Edible films produced up to 27%.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2016-10-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/36
10.14421/biomedich.2016.52.33-40
Biology, Medicine, & Natural Product Chemistry; Vol 5, No 2 (2016); 33-40
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/36/29
Copyright (c) 2016 Endaruji Sedyadi, Syafiana Khusna Aini, Dewi Anggraini, Dian Prihatiningtias Ekawati
oai:ojs.sciencebiology.org:article/327
2023-08-09T16:42:43Z
BIOMEDICH:ART
Antidiarrhoeal Activities of Lime (Citrus aurantiifolia) Extract in Experimentally-Induced Diarrhoea Model
Isirima, Joshua Charles
Uahomo, Precious Ojo
Medicine; Pharmacology; Biomedical Science; Natural Product
Diarrhoea; Citrus aurantiifolia; Castor Oil; Lime juice extract; Gastrointestinal Tract; Enteropooling; Diarrhoea model
This study investigated the effects of Lime on Diarrhoea in Wistar rats. A total of 60 Wistar rats were procured and randomly divided into 3 groups of 20 animals each for each of the three t-test models. The twenty healthy Wistar rats for each diarrhoea model were fasted for 6 hours prior to the experiment but allowed free access to water. The twenty animals were randomly divided into 5 groups of 4 animals each for each experiment. Established antidiarrhea models were followed. The test groups received various doses (97.65mg/kg, 195.3mg/kg, and 390.6mg/kg) of Citrus aurantiifolia juice extract; whereas positive controls received Loperamide (2.5mg/kg) and negative controls received distilled water (1ml/kg). The administration was done once daily for 15 days, and the faeces of each animal was collected on the 5th, 10th and 15th day. The result of this study showed that medium and high dose Citrus aurantiifolia has an anti-diarrhoeal effect on castor oil-induced diarrhoea over repeated administration for a minimum of 15 days as it prolonged the onset of diarrhoea, decreased the frequency of defecation and gastrointestinal transit time in Wistar rats. This study shows that Citrus aurantiifolia demonstrates significant anti-diarrhoeal activity and can be used as an anti-diarrhoea agent.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-03-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/327
10.14421/biomedich.2023.121.305-313
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 305-313
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/327/197
Copyright (c) 2023 Joshua Charles Isirima, Precious Ojo Uahomo
oai:ojs.sciencebiology.org:article/97
2020-04-11T02:49:49Z
BIOMEDICH:ART
Anatomical and Histological Study of Shark (Carcharhinus sorrah) Kidney
Supriyati, Hikmah
Rakhmiyati, Rakhmiyati
Luthfi, Muhammad Ja’far
Sharks; Kidney; Anatomy; Histology
Sharks are sea water fishes belong to the class Chondrichthyes, Subclass Elasmobranchii. Sharks are cartilaginous fish that have a different osmoregulation process than any other sea water fish. Cartilaginous fish is the only vertebrate that can maintain urea. This study aims to determine the anatomical and histological structure of the kidney in the anterior, medial and posterior parts of kidney. The study was conducted by observing anatomy of the kidney. Histological preparations were made using the paraffin method. Qualitative descriptive data analysis was done. Research results show that shark kidneys consist of three parts, namely the head kidney, the body kidney, and the tail kidney. Kidney sharks are brownish red with a size of 18 cm long. Histological observations of shark kidney in the head kidney reveals many glomerulus, body kidney reveals many distal and tubule proximal contractile tubules whereas tail kidney reveals stroma that is rarely found in vertebrate kidney.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/97
10.14421/biomedich.2019.82.37-40
Biology, Medicine, & Natural Product Chemistry; Vol 8, No 2 (2019); 37-40
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/97/86
Copyright (c) 2019 Hikmah Supriyati, Rakhmiyati, Muhammad Ja’far Luthfi
oai:ojs.sciencebiology.org:article/392
2024-03-20T02:04:38Z
BIOMEDICH:ART
Analgesic and Antipyretic Activity of Sweet Orange Peel Methanol Extract
Silalahi, Elsa Debora
Lister, I Nyoman Ehrich
Fachrial, Edy
Analgesic; Antipyretic; Sweet Orange; Peel; Methanol
An analgesic-antipyretic drug widely used is paracetamol, which has various health benefits and several adverse effects. Therefore, various natural products have been extensively studied as alternative analgesic-antipyretics, one of which is sweet orange peel. This study aimed to investigate sweet orange peel's analgesic and antipyretic activity by in vivo methods. This experimental study evaluated the analgesic and antipyretic effects of sweet orange peel extract extracted by the maceration method. The analgesic effect was evaluated by tail immersion (Maximum Possible Analgesia) and acetic acid-induced writhing method (total abdominal writhing). Meanwhile, the antipyretic effect was evaluated by the brewer yeast-induced hyperpyrexia (body temperature) method. This study showed that sweet orange peel methanol extract significantly increased the maximum possible analgesia value (132.79%) and reduced the number of abdominal writhing (44.05%) at the highest dose of 750 mg/kg BW. It indicated analgesic activity from sweet orange peels. Meanwhile, the antipyretic effect of sweet orange peel methanol extract was observed from 1-4 hours after administration, and the highest percentage inhibition of body temperature 4 hours after administration was found in a moderate dose, that was 5.98% (P value: 0.042). Therefore, it can be concluded that sweet orange peel methanol extract has analgesic and antipyretic effects with an optimal dose range of 500-750 mg/kg BW.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/392
10.14421/biomedich.2023.122.577-584
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 577-584
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/392/229
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/392/51
Copyright (c) 2023 Elsa Debora Silalahi, I Nyoman Ehrich Lister, Edy Fachrial
oai:ojs.sciencebiology.org:article/157
2022-07-31T08:19:35Z
BIOMEDICH:ART
A Physical Chemistry Study of Black Powder Materials by Solution Combustion Synthesis Method
Hidayanti, Fitria
Lestari, Kiki R.
Sujani, Nano
Raharjo, Jarot
Chemistry
La2NiO4; Solution Combustion Synthesis; Battery NiMH; Hydrogen Storage Alloy
A study on the synthesis of black powder (La2NiO4) material using the solution combustion synthesis method at a variation of synthesis temperature of 60, 70, and 80°C was carried out. It produces a mass of black powder of 2 grams by four times of synthesis process. Then, material characterization was performed on the black powder samples obtained by using X-ray Diffraction (XRD) to determine the phases formed, Scanning Electron Microscopy - Energy Dispersive X-ray Spectroscopy (SEM-EDS) to determine the morphology and analyze the composition elemental on the microscale and Fourier Transform Infra-Red (FTIR) to determine chemical bonds. From the whole black powder sample, XRD analysis showed the phases of Dilantanum Nickel Tetraoxide (La2NiO4), Nickel Oxide (NiO), Lanthanum Oxide (La2O3), and Lanthanum Oxide Ht x-form (La2O3 Ht (x-form)). In addition, it was seen from the visible compositions of the phases that the NiO phase looks more dominant and the variation of the synthesis temperature shows that the La2O3 phase was increasing. This was supported by the EDS analysis, which showed that the EDS spectrum contains elements La, Ni, and O where the element O indicates that oxidation occurs in the elements Ni and La. On the other hand, the SEM analysis results confirm that the black powder sample contains the elements La and Ni, based on the high and low electron images contained in the morphology of the black powder sample. In addition, it was also known that the particles in the black powder sample were micron size and had porous morphology. This occurs due to rapid thermal decomposition events and excessive gas development. In addition, FTIR analysis showed that the O-H bond had been reduced and there are still C-O and C-H bonds indicating the presence of organic elements possessed by glycine.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-10-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/157
10.14421/biomedich.2021.102.93-103
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 2 (2021); 93-103
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/157/126
Copyright (c) 2021 Fitria Hidayanti, Kiki R. Lestari, Nano Sujani, Jarot Raharjo
oai:ojs.sciencebiology.org:article/260
2023-08-09T16:42:43Z
BIOMEDICH:ART
Solanum anomalum Leaf Extract and Fractions Attenuate Oxidative Stress and Liver Injuries in Alloxan-Induced Diabetic Rats
Etuk, Idongesit Charles
Udobang, John Akpan
Ebong, Nwakaego Omonigho
Okokon, Jude Efiom
Natural Product; Chemotherapy, Toxicology
Solanum anomalum; Medicinal plant; liver protective; antioxidant; antioxidative stress
The leaf of Solanum anomalum used in ethnomedicine for the treatment of various ailments such as diabetes was evaluated for antioxidative stress and hepatoprotective potentials against hepatic injuries in alloxan-induced diabetic rats. Antioxidative stress and hepatoprotective activities of leaf extract and fractions (70-210 mg/kg) were assessed by determining oxidative stress markers levels, liver function indices and histopathological study of livers of treated rats. The leaf extract and fractions caused significant (p<0.05 0.001) increases in the levels of oxidative stress markers (SOD, CAT, GPx, GSH) in the livers of the treated diabetic rats. The extract/fractions treatment caused reduction in liver enzymes (ALT, AST and ALP), total and direct bilirubin. Histology of the livers revealed absence or significant reductions in pathological features in the treated diabetic rats compared to untreated diabetic rats. The results show that the leaf extract and fractions of S. anomalum has antioxidative stress and hepatoprotective potentials which may be due to the antioxidant activities of their phytochemical constituents.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
None
2022-10-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/260
10.14421/biomedich.2023.121.33-44
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 33-44
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/260/165
Copyright (c) 2022 Idongesit Charles Etuk, John Akpan Udobang, Nwakaego Omonigho Ebong, Jude Efiom Okokon
oai:ojs.sciencebiology.org:article/28
2018-08-20T01:52:35Z
BIOMEDICH:ART
Tapak Liman (Elephantopus scaber L) As Immunostimulant and Its Effect on Lymphocyte Differentiation in Mice BALB/C
Kelik, Marmi
Faculty of Languages and Science
Tapak Liman; Immunostimulant; Lymphocyte
Tapak liman (Elephantopus scaber L) is one of the plants that have medicinal properties and has been used for maintenance and improvement of health and disease treatment. The purpose of this study was to determine the effect of extracts of Tapak Liman (Elephantopus scaber L) as immunostimulant to the development of lymphocytes in mice BALB / C. The procedure of this study was to test aqueous extracts in vivo with various treatments (control, treatment of 0.5 g / kg, 1.0 g/ kg, 2.0 g / kg) in healthy mice BALB / C for 2 weeks. After the treatment carried out analysis of the percentage and number of cells that express CD4+, CD8+ and CD4+ CD8 + in thymus organ, using flowcytometry. Analysis of data using one-way ANOVA followed Tukey's test with SPSS. From the analysis showed that the extract of Tapak liman at various doses showed no significant effect on the percentage expression of CD4 + CD8 + and CD4 + CD8 + in thymus organs. While the analysis of the number of cells, extracts of Tapak liman show its effect on the number of cells that express CD4+, CD8 + and CD4 + CD8 + in thymus organs. Concentration of 1.0g / kg of mice showed a good effect on the increase in T helper cells (CD4 +), cytotoxic T cells (CD8 +) and Prothymosit cells (CD4 + CD8 +).
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-10-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/28
10.14421/biomedich.2015.42.49-51
Biology, Medicine, & Natural Product Chemistry; Vol 4, No 2 (2015); 49-51
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/28/16
Copyright (c) 2015 Marmi Kelik
oai:ojs.sciencebiology.org:article/283
2023-08-09T16:42:43Z
BIOMEDICH:ART
The Effects of Frequent Therapeutic Administration of Artesunate-amodiaquine and Artemether-lumefantrine on Haematological Markers in BALB/c Mice
Audu, David
Idowu, Olufunmilayo Ajoke
Patel, Vinood B
Mshelbwala, Musa Fakilahyel
Idowu, Adewumi Babatunde
Medicine; Biology
Malaria; Artemether Lumefantrine; Artesunate Amodiaquine; Hematological parameters; Anemia; Neutropenia
Artemisinin Combination Therapy (ACT) is readily available in malaria-endemic nations, leading to repeated drug usage by undiagnosed persons. Repeated use of ACT therapy by non-infected individuals may affect blood cells. This study explored how repeated artesunate-amodiaquine (A/A) and artemether-lumefantrine (A/L) treatment in non-infected mice affected haematological markers. 100 male BALB/c mice were randomly divided into 5 groups: non-infected and Plasmodium berghei NK65 infected treated with A/L and A/A 1X, 2X, 3X, 4X, 5X, and 6X, and the control group. Packed cell volume (PCV), Haemoglobin (Hb), and red blood cell (RBC) were reduced (p>0.05) non-significantly in the non-infected group treated with A/L or A/A six times compared to the control and infected groups. WBC rose in infected and non-infected mice treated with A/L or A/A 1X, 2X, 3X, and 6X, with a substantial rise in non-infected mice treated with A/L (p < 0.01) and A/A (p < 0.001) three times. WBC mainly rose due to lymphocytes, although neutrophils decreased. Repeated therapeutic use of A/L and A/A without infection may cause a haematological change. Continuous efforts are needed to educate the public about screening for malaria parasites before using drugs.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Nigeria Federal Government Tertiary education trust fund (TETfund)
2023-03-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/283
10.14421/biomedich.2023.121.233-240
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 233-240
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/283/186
Copyright (c) 2023 David Audu, Olufunmilayo A Idowu, Vinood B Patel, Musa F Mshelbwala, Adewumi B Idowu
oai:ojs.sciencebiology.org:article/80
2020-04-11T03:13:43Z
BIOMEDICH:ART
Link of Nasopharyngeal Carcinoma and Epstein-Barr Virus
Sugiyanto, Sugiyanto
Aryati, Lina
Kusumo, Fajar Adi
Hardianti, Mardiah Suci
Nasopharyngeal Carcinoma (NPC); Epstein-Barr Virus (EBV); mathematical model; Invasive Carcinoma Cells
Nasopharyngeal Carcinoma (NPC) is a cancer that occurs in nasopharynx which is associated with Epstein-Barr Virus (EBV). Mutation agents in nasopharyngeal neoplasms occur because of EBV infection. Transformation of B-cells due to EBV causes hormone imbalance in lymphoid cells or nasopharyngeal epithelial tissue. Rates of EBV infection have been shown to be prognostic to NPC. The basic level of EBV DNA can be used for stratification prognosis, with higher titers showing greater disease severity and worse outcomes. With mathematical models, there is a correlation between the increase in Epstein-Barr Virus and the increase in Invasive Carcinoma Cells or increase in Nasopharyngeal Carcinoma Cells.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2018-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/80
10.14421/biomedich.2018.72.51-55
Biology, Medicine, & Natural Product Chemistry; Vol 7, No 2 (2018); 51-55
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/80/68
Copyright (c) 2018 Sugiyanto Sugiyanto, Lina Aryati, Fajar Adi Kusumo, Mardiah Suci Hardianti
oai:ojs.sciencebiology.org:article/395
2024-03-20T02:04:38Z
BIOMEDICH:ART
Liver Protection Effect of Red Rose Petal Methanol Extract in Copper-Induced Liver Injury in Male Wistar Rats
Hutagalung, Caroline Febrina
Girsang, Ermi
Mutia, Maya Sari
Suhartina, Suhartina
Natali, Oliviti
Copper; Rose; SGOT; SGPT; Liver Protection
The human body requires low amounts of copper to maintain homeostasis, but high copper intake can cause various health problems. Many previous studies have been performed to look for natural products that can neutralize the excessive copper effect; one such natural product is a rose petal. Therefore, this study aims to investigate the liver protection effect of rose petals in copper-induced liver injury in male Wistar rats. This experimental study used thirty male Wistar rats that were grouped into five groups, including control, standard (quercetin), Rose Petal Methanol Extract-1 (400 mg/kg BW), 2 (800 mg/kg BW), and 3 (1,200 mg/kg BW). All groups were treated for 14 days, and all rats were given copper (II) sulfate suspension for the last three days. On the fifteenth day, all rats were sacrificed to obtain blood and liver tissue SGOT, SGPT, and liver histology study. The results showed that rose petal methanol extract significantly decreased SGOT and SGPT levels compared to the control group (P-value <0.05). The reduced area of ballooning degeneration and necrosis were in line with the increased dose of rose petal extract supported the liver protection effect of rose petal extract. Therefore, it can be concluded that rose petal methanol extract protects the liver against copper impacts.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-09-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/395
10.14421/biomedich.2023.122.507-513
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 507-513
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/395/215
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/395/53
Copyright (c) 2023 Caroline Febrina Hutagalung, Ermi Girsang, Maya Sari Mutia, Suhartina Suhartina, Oliviti Natali
oai:ojs.sciencebiology.org:article/143
2022-01-11T09:15:08Z
BIOMEDICH:ART
Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media
Naser, Ahmad Saifun
Wisnu, Muhammad
Biological, Biotechnology
BAP (6-Benzyl Amino Purine); krisan (Chrysanthemum morifolium Ramat cv Dewi ratih); plant tissue; alternative media (Growmore, Gandasil D and Mutiara)
Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-07-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/143
10.14421/biomedich.2020.92.109-115
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 2 (2020); 109-115
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/143/111
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/143/27
Copyright (c) 2020 Ahmad Saifun Naser, Muhammad Wisnu
oai:ojs.sciencebiology.org:article/208
2022-09-20T02:36:50Z
BIOMEDICH:ART
Anti-oxidant, Anti-inflammatory and Anti-Atherosclerotic Activity of Bioactive Peptide HPAEDR Isolated from Catla catal Muscle on LPS Induced Inflammation on 246.7RAW Macrophage Cells and HCF Induced Hyperlipidemic Zebrafish Larvae
Sethuramalingam, Sabarinathan
Ravi, Revathy Leena
Rajiah, Janet Rani
Biology; Biotechnology; Medicine; Chemistry
anti-inflammatory; antioxidant; peptide; Zebrafish larvae; 246.7RAW macrophage; inflammation
A muscle wasdissected from the Catla catlafish and enzyme hydrolysis was done using various digestive enzymes such as pepsisn, protease, papine, trypsin and alcalase at verity of time intervals (0th, 3rd, 6th, 9th and 12th) hour respectively. Followed by, the amino acid composition was identified and the confirmative assays such as, the anti-oxidant assays (DPPH and Hydroxy radical scavenging activity) and anti-inflammatory assays (HRBC and AD) were done for various peptide hydrolysate. The active hr was identified as 9th hr alcalase hydrolysate which was purified through Ultrafiltration (>30 kDa, 30-10 kDa, 10-3 kDa and <3 kDa). These fractions were again studied for its anti-oxidant and anti-inflammatory activity. Based on the results obtained, the active fraction was identified as 10-3kDa which was further purified and identified using Gel filtration chromatography and LC-MS/MS as HPAEDR (723.76 Da). Further, for in vitro and in vivo studies the peptide derived from CCM was synthetically designed with 98% purity (PhtdPeptides Co., Ltd. Zhemgzhou, China). Additionally, the physiochemical properties (Solubility, emulsifying properties and foaming properties) of these fraction was studied. Finally, the purified fraction was tested for in vitro activity through cell viability, COX-2 production, NO production and TNF-α production. Moreover, the in vivo protective effect is tested on Zebrafish larvae. The results suggest that the active purified peptide fraction isolated from Catla catla muscle has a strong natural anti-oxidant, anti-inflammatory and cholesterol reduction activity which can be used in functional foods and pharmaceuticals.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-08-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/208
10.14421/biomedich.2022.112.151-160
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 2 (2022); 151-160
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/208/153
Copyright (c) 2022 Sabarinathan Sethuramalingam, Revathy Leena Ravi, Janet Rani Rajiah
oai:ojs.sciencebiology.org:article/271
2023-08-09T16:42:43Z
BIOMEDICH:ART
Immunoprotective Effect of Cocos nucifera Oil on Sheep Red Blood Cell-Induced Immunocompromised Rats
Idu, MacDonald
Debby, Igori
Gabriel, Benjamin Ogunma
Phytomedicine/ pharmacology
Immunoprotective; Cocos nucifera; Sheep red blood cell; Immunocompromised
The aim of this study is to investigate the immunoprotective effects of cold-pressed coconut oil in immunocompromised rat. Standard procedure was used to perform this research work with a modified method. The effect of immunomodulatory properties of coconut oil was evaluated after challenging the animals with 0.3 ml sheep red blood cells (SRBCs) intraperitoneally and further treated with graded doses (0.25, 0.5 and 1.0 ml/kg i.p) of the oil extract for 21 days. The hematological, CD4, CD8 biochemical and histopathological analysis were evaluated. Result obtained from this study showed a significant increase in hematological indexes (WBC: 11.70; LYM: 9.9 GRAN: 8.0 and HGB: 15.57) across the treated groups, but majorly at lowest dose (0.25 ml/kg). More so a significant increase in CD4 and CD8 Count specifically at 0.25 ml/kg (7.763 and 7.830). Anti-oxidant property of the oil extract at 0.25 ml/kg had a significant reduction in malondialdehyde with an increased in antioxidant enzymes (SOD, CAT and GPx) when compared with untreated control. The body weight of the animals in the treated groups showed a significant increase at 0.25 ml/kg of the oil extract when compared with the untreated control. Liver function test (ALT, AST and ALP) showed no significant increase in the treatment groups when compared with the controls. The histopathological result reveals a normal physiological structure of the heart, lungs, spleen, liver and kidney in lowest dose of the oil extract. In conclusion, this study validated the ethnomedicinal property of the plant.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
NON
2023-01-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/271
10.14421/biomedich.2023.121.159-169
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 159-169
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/271/177
Copyright (c) 2023 MacDonald Idu, Igori Debby, Benjamin Ogunma Gabriel
oai:ojs.sciencebiology.org:article/52
2018-08-20T01:50:51Z
BIOMEDICH:ART
On Designing Interactive Online Atlas of Reptile Anatomy (Mabouya multifacsiata)
Luthfi, Muhammad Jafar
Riyanto, Riyanto
AtlasAnatomy.org; online anatomy atlas; vertebrates; reptile; interactive atlas
This research is an integration between fields of Biology, Photography, Design, and Informatics Engineering. The study aimed to build an interactive online atlas of reptile anatomy to improve the accessibility and data sharing (free access) of reptile anatomy. Website was developed using SDLC (System Development Life Cycle) which consist of five steps as follows: website’s strategic planning, determine the scope of website, website’s requirements analysis, design and implementations of website, and testing. Based on the results of testing and system implementation, it can be concluded that online interactive atlas (AtlasAnatomy.org) had been successfully built as anatomical educational media of reptile.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2017-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/52
10.14421/biomedich.2017.62.63-69
Biology, Medicine, & Natural Product Chemistry; Vol 6, No 2 (2017); 63-69
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/52/43
Copyright (c) 2017 Muhammad Jafar Luthfi, Riyanto Riyanto
oai:ojs.sciencebiology.org:article/377
2024-03-20T02:04:38Z
BIOMEDICH:ART
Chemical Properties of Liquid Broth Extracted from Freshwater and Marine Shrimp Shells Waste
Fadhallah, Esa Ghanim
Koesoemawardani, Dyah
Indraningtyas, Lathifa
chemical; liquid broth; protein; shrimp shell; waste
Indonesia's shrimp industry is growing rapidly, but a surge in shrimp waste such as shells and heads shrimps are increasing as well. These waste products contain important components such as protein, minerals, and amino acids. This study aims to determine the chemical properties of liquid broth extracted from freshwater and marine shrimp shells, including ash, protein, fat, monosodium glutamate (MSG), and antioxidant. The liquid broth was extracted by boiling shrimp shells and heads in water with a ratio of 1:2 for 1 hour at 80oC. Results indicate that the type of shrimp used did not affect the broth's ash, fat, protein, MSG, or antioxidant content. Marine and freshwater shrimp liquid broths contain 0.56% and 0.28% ash, 0.10% and 0.50% fat, 2.19% and 1.97% protein, 1.5291% and 1.6274% MSG, and 2263.73 ppm and 2786.2 ppm antioxidant.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-08-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/377
10.14421/biomedich.2023.122.437-440
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 437-440
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/377/209
Copyright (c) 2023 Esa Ghanim Fadhallah, Dyah Koesoemawardani, Lathifa Indraningtyas
oai:ojs.sciencebiology.org:article/112
2021-07-22T06:52:01Z
BIOMEDICH:ART
Non-Alkaloidal Compounds from Khat (Catha edulis) Leaves
Kiros, Tsegu
Natural Product Chemistry
Khat; Catha edulis; non-alkaloid; KNA-1; KNA-2
Khat belongs to the family Celastraceae, genus Catha, and species edulis. More than 200 compounds have previously been identified in Khat leaves, including: 40 alkaloids, terpenoids and sterol, flavonoids, glycosides, tannins, amino acids, vitamins and minerals. Researchers have spent their effort and time merely on study of the alkaloidal components (mainly the stimulant agents, cathinone and cathine) of Khat both qualitatively and quantitatively. The two principal Khat stimulant compounds, cathinone and cathine, by now are well established. But, on the contrary, previous studies on the non-alkaloidal constituents of the plant were limited. The objective of this work was therefore to isolate and characterize compounds from non-alkaloidal fractions of the plant’s leaves. In this work, two non-alkaloidal compounds (KNA-1 and KNA-2) were isolated and characterized from the acid-EtOAc extract of fresh and oven-dried leaves of Khat (Catha edulis). From the present study, it is possible to conclude that investing more effort and time on searching additional non-alkaloidal principles from the leaves of Khat is so necessary. And further works could be done in the future to isolate extra non-alkaloidal compounds from the leaves and other parts of Khat and evaluate their biological activity.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Ethiopian Ministry of Education, Addis Ababa University
2020-11-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/112
10.14421/biomedich.2020.92.81-89
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 2 (2020); 81-89
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/112/102
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/112/7
Copyright (c) 2020 Tsegu Kiros
oai:ojs.sciencebiology.org:article/418
2024-03-20T02:04:38Z
BIOMEDICH:ART
Bioactivity of Vernonia arborea Leaves from Bentian Tribe as Natural Antioxidant and Antibacterial Based on Local Knowledge
Sari, Nur Maulida
Aziza, Humairo
Aryani, Farida
Murdianto, Murdianto
Natural Products; Natural Product Chemistry; Ethnobotany; Bioinformatics
Vernonia arborea; antibacterial; DPPH; traditional medicine
The utilization of natural organic materials as raw materials for standardized medicine, including the use of organic materials in medicine manufacture are currently being encouraged by the Government. Based on the information from Bentian Tribe people, Vernonia arborea leaf or Kutu Bu’ut leaf has the potential to be developed as natural medicine derived from plants. This study explored the potential of V.arborea leaf for its phytochemicals screening by Harborne, Kokate and Senthilmurugan method, antioxidant analysis was evaluated by DPPH radical scavenging assay. Antibacterial analysis was examined using agar well diffusion method against Escherichia coli and Propionibacterium acnes. The results showed that the V.arborea leaf contained alkaloid on n-hexan extract and coumarin on ethyl acetate extract. Ethanol extract from V.arborea leaf contained alkaloid, flavonoid, saponin, tannin, and carbohydrate. Antioxidant activity showed that the highest inhibition by 83% at 50 ppm consentration of ethanol solvent. Antibacterial activity of E. coli and P.acnes showed that the highest inhibition zone by 12 mm and 11 mm at 400 µg/well of ethanol extract. Based on the results, the V.arborea leaf contains natural bioactivity and has potential to be further developed as a natural traditional medicine.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Ministry of Education, Culture, Research and Technology
2024-03-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/418
10.14421/biomedich.2023.122.675-680
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 675-680
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/418/247
Copyright (c) 2024 Nur Maulida Sari, Humairo Aziza, Farida Aryani, Murdianto
oai:ojs.sciencebiology.org:article/177
2022-07-31T01:51:06Z
BIOMEDICH:ART
Ethnomedicinal, Phytochemicals, and Pharmacological Aspects of Sentul (Sandoricum koetjape)
Wijaya, Made Dharmesti
Natural Product; Biology; Chemistry; Pharmacy; Medicine;
Ethnomedicine; Pharmacology; Phytochemicals; Sandoricum koetjape; Sentul
Sentul (Sandoricum koetjape) is a tropical plant that has been used as traditional medicine in some Asian countries for decades. Research on phytochemicals and pharmacological activities of this plant extracts has been conducted and shows promising medicinal properties. This review aims to integrate knowledge about S. koetjape focusing on three main aspects namely ethnomedicinal, phytochemicals, and pharmacological, in order to encourage further research on this plant for future drug development. Traditionally, all plant parts of S. koetjape have been used for treating various health problems and diseases such as diarrhea, fever, colic, and leucorrhoea. More than 30 chemicals have been identified from S. koetjape, which the most important compounds are ring-A secotriterpene, oleane-type triterpene, secomultiflorane-type triterpene, hydroxymultiflorane triterpene, and limonoids. In vitro studies showed pharmacological potential of the extracts and phytochemicals constituents of S. koetjape including antibacterial, antifungal, antitumoral, anticancer, insecticide, and antioxidant.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-04-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/177
10.14421/biomedich.2022.111.65-73
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 1 (2022); 65-73
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/177/141
Copyright (c) 2022 Made Dharmesti Wijaya
oai:ojs.sciencebiology.org:article/19
2018-08-20T01:52:56Z
BIOMEDICH:ART
A Discovery and Characteristics Description of Telosma puberula (Asclepiadoideae) in Mount Gedang Atas and Mount Ijo, Baturagung Mountain Yogyakarta
Widodo, Widodo
Telosma puberula; Asclepiadoideae; Mount Gedang Atas; Baturagung Mountains
Population of Telosma puberula in community forest bushes was identified at S.07.48'.44.1"; E.110.31.15.8", 411m, Mount Gedang Atas and S 070 04 '04.1"; E 1100 30 '47.9 ", 415m, Mount Ijo. Telosma puberula was also found in Mount Parangan, Mount Mintorogo, Mount Nglanggeran, and Baturagung Mountains Yogyakarta. The identification was based on herbarium specimens collected by Horsfield in 1802 and 1859 from Java island, Indonesia (K000873052, K000873053). Information about Telosma puberula is very limited. This article describe photographs of morphological characters of the plant’s stems, leaves, flowers, and pollinia.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-10-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/19
10.14421/biomedich.2014.32.47-52
Biology, Medicine, & Natural Product Chemistry; Vol 3, No 2 (2014); 47-52
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/19/7
Copyright (c) 2014 Widodo
oai:ojs.sciencebiology.org:article/43
2018-08-20T01:51:45Z
BIOMEDICH:ART
Antioxidant Capacity Comparison of Ethanolic Extract of Soursop (Annona muricata Linn.) Leaves and Seeds as Cancer Prevention Candidate
Widyastuti, Dyah Ayu
Rahayu, Praptining
Annona muricata Linn.; Antioxidant capacity; DPPH; extraction
Annona muricata Linn. (soursop) is one of tropical plants which have relatively complete chemical compounds. It has flavonoid, tannin, phytosterol, alkaloid, etc. The high antioxidant compound in soursop is believed as cancer prevention so the cancer threat in the world can be minimized. The antioxidant compound in soursop can be found not only in its fruit, but also in other parts like leaves, seeds, etc. Based on that potency, this study aimed to compare antioxidant capacity of soursop leaves and seeds, also to study about the utilization of soursop parts which is usually not used. This research began with maceration to extract leaves and seeds with 96% ethanol. Ethanolic extract of soursop leaves and seeds were then tested for antioxidant capacity with DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The result showed that antioxidant capacity of soursop leaves and seeds are 85,66875% and 39,0166, respectively. The antioxidant capacity of leaves is higher than seeds due to seed’s extraction difficulty so its antioxidant compound could not be extracted optimally. However, either leaves or seeds extract in this study are potential as antioxidant resources because there are no significant differences between antioxidant capacity of both extract.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2017-04-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/43
10.14421/biomedich.2017.61.1-4
Biology, Medicine, & Natural Product Chemistry; Vol 6, No 1 (2017); 1-4
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/43/34
Copyright (c) 2017 Dyah Ayu Widyastuti, Praptining Rahayu
oai:ojs.sciencebiology.org:article/346
2023-08-12T13:44:13Z
BIOMEDICH:ART
Nanotechnology-Based Vaccines
Hussein, Mohamed
Mumtaz, Madiha
Nasir, Iqra
Abdullahi, Anisa
Nanoparticles; Immunogen; Vaccines
Several new infectious diseases have developed in recent years, and a few old ones that were formerly thought to pose no threat to humans have made a comeback. Millions of fatalities are attributed to these illnesses together, having a significant negative influence on the worldwide socioeconomic and healthcare sectors. The lack of appropriate medications for many of these disorders is one of the biggest obstacles to treating them. Yet, several of the most common diseases currently have no vaccinations that are reliable. The ideal vaccine should have several key characteristics, including safety, stability, and the capacity to induce a sufficient and long-lasting immune response with a minimal number of doses. To induce protective immunity against illnesses, different generation vaccines are employed, including attenuated or dead entire organisms (first generation), subunits (second generation), and RNA or DNA vaccines (third generation). To get beyond these obstacles, a reliable vaccination delivery mechanism is needed, one that not only gets the vaccine molecules to the target region where they can trigger long-lasting immune responses but also has few side effects and uses fewer doses. Only a few hundred atoms make up the majority of nanoparticles. Nanoparticles have a relatively significant surface area-to-volume ratio because of their extremely small size. Nanoparticles can have surprising optical, physical, and chemical properties due to this property. Nanotechnology has many benefits for the creation of vaccines for the next generation. A delivery strategy based on nanocarriers can shield vaccines from early deterioration, increase stability, have high adjuvant qualities, and can help with the targeted distribution of an immunogen. The researcher conducts an examination of articles that are in accordance with the issue to be studied. Articles used in the literature review are obtained through the database of international journal providers through PubMed, we investigated clinical studies and discussed what happened in these clinical studies and the extent of the effectiveness of Nanoparticle-Based Vaccines. In order to achieve effective vaccine distribution and generate the required host immunity against infectious diseases, this review article focuses on the applications of nanocarrier-based vaccine formulations and the methodologies utilized for functionalizing nanoparticles.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-06-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/346
10.14421/biomedich.2023.121.343-361
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 343-361
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/346/201
Copyright (c) 2023 Mohamed Hussein, Madiha Mumtaz, Iqra Nasir, Anisa Abdullahi
oai:ojs.sciencebiology.org:article/103
2021-07-21T20:50:54Z
BIOMEDICH:ART
A Mathematical Model of the Covid-19 Cases in Indonesia (Under and Without Lockdown Enforcement)
Sugiyanto, Sugiyanto
Abrori, Muchammad
Corona virus novel 2019 (COVID-19); mathematics model; lockdown; Indonesia
COVID-19 stands for Corona (CO), Virus (VI), Disease (D) and year 2019 (19), which is COVID-19 first appeared in 2019. Mathematical model of covid deployment in Indonesia under and without lockdown case uses the SIRV model, such as Susceptible, Infected, Recovery, and Virus. The results of this model state that under lockdown the spread of COVID-19 could be stopped. If it were not under lockdown it can multiply 1,276 times higher over two months.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2020-04-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/103
10.14421/biomedich.2020.91.15-19
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 1 (2020); 15-19
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/103/92
Copyright (c) 2020 Sugiyanto, Muchammad Abrori
oai:ojs.sciencebiology.org:article/408
2024-03-20T02:04:38Z
BIOMEDICH:ART
Effect of Citrullus lanatus Seeds Extract on Haematological Parameters After Administration with Medroxyprogesterone Acetate on Female Wistar Rats
Nnumolu, Michael Chuks
Physiology; Reproductive Physiology; Haematology
Medroxyprogesterone acetate; Citrullus lanatus seeds; Haematology; Blood Physiology
This study was carried out to investigate the effect of Citrullus lanatus seeds extract on the haematological parameters after administration with medroxyprogesterone acetate on female Wistar rats. Twenty-five rats weighing 170-230 g were divided into five groups containing five rats each. Group I served as control and received only rat chow and water; Group II received rat chow, water and a high dose of MPA only; Group III received rat chow, water, a high dose of MPA and a high dose of extract; group IV received rat chow, water, high dose of MPA and a low dose of extract, and group V received rat chow, water and high dose of extract only. The administration lasted for twenty-eight days after two weeks of acclimatisation. On the twenty-ninth day, the animals were anaesthetised using chloroform, sacrificed and their blood samples obtained via cardiac puncture for haematology analysis. The results showed that the level of haematological parameters significantly increased (p<0.05) in the rats that received a high dose of MPA and a high dose of Citrullus lanatus seed extract compared to the control group. This suggests that MPA affects bone marrow, which produces blood cells, significantly decreasing platelet count, white blood cell count, red blood cell count, haemoglobin count, packed cell volume, mean corpuscular volume, and mean corpuscular haemoglobin concentration. This study also shows that an aqueous extract of Citrullus lanatus seed is potent in increasing the level of haematological parameters when co-administered with MPA.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-11-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/408
10.14421/biomedich.2023.122.611-617
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 611-617
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/408/236
Copyright (c) 2023 Michael Chuks Nnumolu
oai:ojs.sciencebiology.org:article/165
2022-07-31T08:19:35Z
BIOMEDICH:ART
Antibacterial Screening of Bacterial Isolates Associated with Mangrove Soil from the Ngurah Rai Mangrove Forest Bali
Indraningrat, Anak Agung Gede
Wijaya, Made Dharmesti
Suryanditha, Putu Arya
Siskayani, Ayu Savitri
Janurianti, Ni Made Defy
Natural Product; Medicine; Microbiology; Bioprospecting
mangrove soil; bacteria; antibacterial screening
In this study we reported cultivation of bacteria associated with mangrove soil from the Ngurah Rai Mangrove Forest, Bali. Mangrove soil samples were serially diluted using sterile artificial seawater, spread onto Starch Casein M agar and incubated at 28oC for 28 days. Cultivation of mangrove soil samples yielded 165 bacterial colonies with 68 isolates were selected and purified based on different morphology. Of these 68 isolates, 22 isolates displayed antibacterial activities ranging from weak to strong inhibition against at least one of four bacterial indicators namely Staphyloccocus aureus, Streptococus mutans, Escherichia coli and Klebsiella pneumoniae using perpendicular streak method. Overall, 19 out of 22 bacteria isolates displayed weak antibacterial potential and two isolates exhibited moderate antibacterial activity. The isolate SA4 was the only bacterium with strong antibacterial potential with measured clear distance ≥ 10 mm against the four bacterial isolates. Sequence analysis based on 16S rRNA gene fragment assigned the isolate SA4 as Bacillus subtilis strain BIL/BS-168. Overall, this study confirmed the untapped potential of antibacterial activities from bacteria associated with mangrove soil.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-11-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/165
10.14421/biomedich.2021.102.129-133
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 2 (2021); 129-133
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/165/130
Copyright (c) 2021 Anak Agung Gede Indraningrat, Made Dharmesti Wijaya, Putu Arya Suryanditha, Ayu Savitri Siskayani, Ni Made Defy Janurianti
oai:ojs.sciencebiology.org:article/232
2023-08-09T16:42:43Z
BIOMEDICH:ART
Spermicidal, Antifertility and Contraceptive Effect of Azadirachta indica A. Juss. Seed Extract in Female and Male Wistar Rats
Idu, MacDonald
Ogedegbe-George, Sharon
Oriarewo, Precious Eromosele
Gabriel, Benjamin Ogunma
Phytomedicine/ pharmacology
Spermicidal; contraceptive; Azadirachta indica; Wistar rats
This study investigates the spermicidal and contraceptive effect of Azadirachta indica seed aqueous extract in male and female Wistar rats. The animals were divided into four groups (n=6), graded doses (2.5, 5, and 10 mg/kg) of the extract. They were exposed to female rats in a ratio of 2:1 after 24 hours, 7, and 14 days of administration. The spermicidal properties were evaluated. The female contraceptive study involved 14 days of pre-coital and post-coital administration of the neem seed extract, and contraceptive indexes were investigated. The results obtained from the spermicidal activity showed a significant decrease in male hormonal levels at 2.5, 5, and 10 mg/kg of A. indica (0.73, 0.50, and 1.08 ng/ml); and the sperm cells (102.5, 111.5, and 97 counts) after 24 hours. Administration and mating, compared to the control. Also, the histopathology of the testes showed normal testicles. The contraceptive study elicited a significant decrease in estrogen (1.46 ng/ml), luteinizing hormone (0.094 mg/dl) and progesterone (1.82 ng/ml), at 5 mg/kg in day 14 post-coital study compared with the control. The histopathology of the treated uterus had no deteriorating effect compared with the control. The extract elicited spermicidal and contraceptive potential at a reduced dose, validating its folklore claim.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
non
2022-11-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/232
10.14421/biomedich.2023.121.79-88
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 79-88
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/232/163
Copyright (c) 2022 MacDonald Idu, Sharon Ogedegbe-George, Precious Eromosele Oriarewo, Benjamin Ogunma Gabriel
oai:ojs.sciencebiology.org:article/33
2018-08-20T01:52:19Z
BIOMEDICH:ART
Local Stability Analysis of a Mathematical Model of the Interaction of Two Populations of Differential Equations (Host-Parasitoid)
Anggreini, Dewi
Mathematical
Mathematical Model; The interaction of two Populations Model; Stability
Mathematical model has many benefits in life, especially the development of science and application to other fields. The mathematical model seeks to represent real-life problems formulated mathematically to get the right solution. This research is the application of mathematical models in the field of biology that examines the interaction of the two populations that host populations and parasitoid populations. This study differs from previous studies that examine the interaction of two more species that prey and predators where predators kill prey quickly. In this study the parasitoid population slowly killing the host population by living aboard and take food from the host population it occupies. In this study of differential equations are used to construct a mathematical model was particularly focused on the stability of the local mathematical model of interaction of two differential equations that host and parasitoid populations. Stability discussed in this study are stable equilibrium points are obtained from the characteristic equation systems of differential equations host and parasitoid interactions. Type the stability of the equilibrium point is determined on the eigenvalues of the Jacobian matrix. Analysis of stability is obtained by determining the eigenvalues of the Jacobian matrix around equilibrium points. Having obtained the stable equilibrium points are then given in the form of charts and portraits simulation phase to determine the behavior of the system in the future.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2016-04-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/33
10.14421/biomedich.2016.51.9-14
Biology, Medicine, & Natural Product Chemistry; Vol 5, No 1 (2016); 9-14
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/33/24
Copyright (c) 2016 Dewi Anggreini
oai:ojs.sciencebiology.org:article/301
2023-08-09T16:42:43Z
BIOMEDICH:ART
Phytochemical and Antioxidant Activity of Blumea balsamifera and Cordyline fruticosa Based on Ethnopharmacology Knowledge of Muara Tae Tribe, East Kalimantan
Sari, Nur Maulida
Aryani, Farida
Wartomo, Wartomo
Hernandi, Muhammad Fikri
Rositah, Erna
Prayitno, Joko
Natural Products; Natural Product Chemistry; Ethnobotany; Bioinformatics
Blumea balsamifera; Cordyline fruticosa; DPPH; Ethnopharmacology; Phytochemical
Plant use as traditional medicine is still widely practiced in Indonesia. Muara Tae tribe people, West Kutai regency are one of the regions that still rely on Blumea balsamifera and Cordyline fruticosa plants as traditional medicine. This study aims to determine the potential of Blumea balsamifera and Cordyline fruticosa leaves as medicinal plants with phytochemicals and antioxidants. Phytochemical analysis was tested using Harborne and Kokate methods. Antioxidant activity was evaluated by DPPH radical scavenging assay with slight modification. The results of the phytochemical analysis showed that the extracts of n-hexane, ethyl acetate, and ethanol from the leaves of Blumea balsamifera and Cordyline fruticosa contained alkaloids, tannins, and triterpenoids. Antioxidant activity of Blumea balsamifera leaves extract showed that the n-hexane extract display an ability to inhibit DPPH free radical by 50% at 100 ppm concentration, while ethyl acetate and ethanol extracts display an ability by 77% and 81% at 50 ppm concentration. IC50 value of ethyl acetate and ethanol extracts of Blumea balsamifera leaves sequentially were 23.68 g/mL and 17.59 g/mL. Antioxidant activity of Cordyline fruticosa leaves extract showed that the n-hexane and ethyl acetate extract display an ability to inhibit DPPH free radical by 45% and 56% at 100 ppm concentration, while ethanol extracts display an ability by 76% at 50 ppm concentration. IC50 value of ethyl acetate and ethanol extracts of Cordyline fruticosa leaves sequentially were 73.72 g/mL and 20.17 g/mL. Based on the results, Blumea balsamifera and Cordyline fruticosa leaves extracts had the potential to develop as natural antioxidants.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Ministry of Education, Culture, Research and Technology
2023-03-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/301
10.14421/biomedich.2023.121.273-280
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 273-280
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/301/194
Copyright (c) 2023 Nur Maulida Sari, Farida Aryani, Wartomo, Muhammad Fikri Hernandi, Erna Rositah, Joko Prayitno
oai:ojs.sciencebiology.org:article/98
2020-04-11T02:49:49Z
BIOMEDICH:ART
Digital Anthropometer Development for Improving the Measurement Quality of Human Body Dimensions
Kusuma, Trio Yonathan Teja
Wirabhuana, Arya
Yusuf, Faurosi Syafa’atul
Application; Digital Anthropometer; Quality; Process Capability
Digital information technology has become the nerve of information for industry-based companies. However, there are still data or information that retrieved manually, one of them is measurement of the human body dimension. The problem is seemed solved by invention of digital anthropometer application. Yet, the application requires a further improvement especially in its accuracy and user interface. The improvement of accuracy quality of its measurement uses the capability process analysis method. As a result, the current Cp and Cpk is 0.26 and -0.209 while in the developed application is 2.56 and 1.218. Thus, the developed application is stated that quality of measurement increases to meet the criteria of good process capability. The user interface is improved based on the user voices. Data storage databases and percentile calculation in the application was added as one on the significant user-interface improvement.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/98
10.14421/biomedich.2019.82.27-32
Biology, Medicine, & Natural Product Chemistry; Vol 8, No 2 (2019); 27-32
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/98/82
Copyright (c) 2019 Trio Yonathan Teja Kusuma, Arya Wirabhuana, Faurosi Syafa’atul Yusuf
oai:ojs.sciencebiology.org:article/364
2024-03-20T02:04:38Z
BIOMEDICH:ART
Endophytic Fungi Isolated from Pteridium acquilinium and Newbouldia laevis Leaves Exhibited Antioxidant Activities and Inhibitory Potential Against Selected Clinical Bacteria Isolates
Ajah, Obinna
Unegbu, Chika Cecilia
Uzohuo, Emmanuel N
Anyanwu, Ogechi Ozioma
Okezie, Ugochukwu Moses
Anyanwu, Chioma
Joseph, Rich
Endophytes; antibacterial activity; antioxidant potential; Pteridium acquilinium Newbouldia laevis
Endophytic fungi are important components of plant micro-ecosystems and they exist usually inside the healthy tissues of living plants. Endophytes possess encouraging source of biologically active metabolites for pharmaceutical applications. This study isolated endophytic fungi from Pteridium acquilinium and Newbouldia laevis leaves and evaluated their antioxidant and antibacterial activities. A total of ten endophytic fungal species were isolated from the leaves of the plants, from which six were selected and identified, including; Epicoccum thailandicum; Trichoderma atroviride; Lasiodiplodia parva; Trichoderma yunnanense; Colletotrichum cobbittiense; and, Trichoderma crissum. Among the isolated fungi, Trichoderma crissum (266.2956 ± 84.84 µg/ml) and Lasiodiplodia parva (293.0755 ± 64.95 µg/ml) showed significant scavenging activity on comparison with ascorbic acid. Furthermore, the isolated endophytes showed potential antibacterial activity against the tested clinical pathogens. This study validates that endophytic fungi dwelling in the inner tissue of medicinal plants studied could be a potential source of biologically active metabolites with free radical scavenging activities and also for treatment of infectious diseases caused by Staphylococcus aureus, Pseudomonas aeroginosa and Escherichia coli.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Tertiary Education Trust Fund (TETFund) Nigeria
2023-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/364
10.14421/biomedich.2023.122.547-553
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 547-553
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/364/219
Copyright (c) 2023 Obinna Ajah, Chika Cecilia Unegbu, Emmanuel N Uzohuo, Ogechi Ozioma Anyanwu, Ugochukwu Moses Okezie, Chioma Anyanwu, Rich Joseph
oai:ojs.sciencebiology.org:article/144
2022-01-11T09:15:52Z
BIOMEDICH:ART
Effect of Ethanol Extracts of Musa paradisiaca Fruit Pulp and Peels on Haematological Indices and Liver Enzymes of Experimental Rats
Moke, Emuesiri Goodies
Umukoro, Emuesiri Kohworho
Ojugbeli, Evelyn Tarela
Ezedom, Theresa
Daubry, Tarela Melish Elias
Omorodion, Iziegbe Lisa
Pharmacology; Toxicology; Ethnopharamocology
Anaemia; Haematology; Liver Enzymes; Medicinal Plants; Musa paradisiaca
Medicinal plants usage in traditional medicine has risen over the years. Musa paradisiaca has been reported that it possesses various therapeutic efficacies. This study is aimed at evaluating the effect of parts of the ethanol fruit extracts of Musa paradisiaca on haematological indices and serum liver enzymes. Thirty Wistar rats were grouped into five groups of six animals each which were administered 200 mg/kg and 400 mg/kg of Musa paradisiac fruit (MPF) pulp extract or peel extract. Haematological indices and liver enzymes were assayed for at the end of the 14-days experimental period. MPF pulp and MPF peel at 400 mg/kg showed a significant (P<0.05) increase in red cell count and haematocrit level as compared to the control. There was a non-significant (P>0.05) increase in haemoglobin concentration in the treated rats as compared to the control. There was also a non-significant (P>0.05) change in AST, ALT, and ALP level of the treated rats as compared to the control. Fruit pulp and peel of Musa paradisiaca improve erythrocytes count and haematocrit level, and they may not be associated with liver toxicity.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-07-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/144
10.14421/biomedich.2021.101.67-71
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 1 (2021); 67-71
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/144/121
Copyright (c) 2021 Emuesiri Goodies Moke, Emuesiri Kohworho Umukoro, Evelyn Tarela Ojugbeli, Theresa Ezedom, Tarela Melish Elias Daubry, Iziegbe Lisa Omorodion
oai:ojs.sciencebiology.org:article/178
2023-08-09T16:42:43Z
BIOMEDICH:ART
High Performance Liquid Chromatography (HPLC) for Detection of Glucosamine and Chondroitin Sulfate Compounds
Rakhmiyati, Rakhmiyati
Widiyani, Tetri
Budiharjo, Agung
Biosain
HPLC; shark; glucosamine; chondroitin sulfate
Glucosamine and chondroitin sulfate are compounds found in shark cartilage (Carcharhinus sorrah). The two compounds have many health benefits, that is wound healing and helping the process of angiogenesis. This study aims to determine the content of glucosamine and chondroitin sulfate compounds in shark cartilage (SC) extract. The method used was High Performance Liquid Chromatography (HPLC) with potassium phosphate buffer solution at pH 3. The results of this research were SC extract contained glucosamine and chondroitin sulfate compounds with a retention time of 1.914 minutes.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-09-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/178
10.14421/biomedich.2023.121.5-8
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 5-8
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/178/158
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/178/30
Copyright (c) 2022 Rakhmiyati, Tetri Widiyani, Agung Budiharjo
oai:ojs.sciencebiology.org:article/296
2023-08-09T16:42:43Z
BIOMEDICH:ART
Comparative Cough Suppression of Chitosan Crab Extract of Uca tangeri and Dihydrocodeine
Isirima, Joshua Charles
Uahomo, Precious Ojo
Medicine; Pharmacology; Biomedical Science; Natural Product
Cough; Suppression; Uca tangeri; Chitosan Crab; Dihydrocodeine, Comparative
Cough is an inmate primitive reflex and acts as a part of the body's immune system to protect against foreign materials from the respiratory tract. This study was done to investigate the cough suppression potential of Uca tangeri. A day before the test, guinea pigs were placed individually in a transparent chamber (60 36 60 cm) for 5 minutes before cough was induced by exposure to 15% citric acid, delivered using an Omron compressor nebulizer (rate of 0.4 ml/minutes and particle size 5?m) for 10 minutes. The animals were then monitored visually within this exposure time for cough; the latency and counts, of which, were taken as the basal values. The animals exhibiting 10 - 20 bouts of cough were selected for the study and fasted overnight but with access to water. The selected animals were randomly allotted to 5 groups (n=5 per group). The animals were treated orally thus: Group 1 was the control group and received 2 ml/kg of normal saline; group 2 received 25 mg/kg dihydrocodeine; Group 3 received 150 mg/kg extract; group 4 received 300 mg/kg extract and group 5 received 600 mg/kg of the extract. An hour after administration, they were re-exposed to citric acid aerosol (as earlier described) and the latency of cough and cough count were recorded. The procedure was repeated at hours 2 and 3 after treatment. Antitussive activity was then evaluated in each guinea-pig as the percentage reduction in the number of coughs also known as percentage suppression of cough and percentage increase in latency of cough. The results revealed that Uca tangeri exhibited a dose dependent percentage increase in cough latency period as well as percentage increase in suppression of cough which was inferior to dihydrocodeine, but significantly greater than normal saline and basal levels.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-02-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/296
10.14421/biomedich.2023.121.197-203
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 197-203
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/296/182
Copyright (c) 2023 Joshua Charles Isirima, Precious Ojo Uahomo
oai:ojs.sciencebiology.org:article/76
2018-08-20T01:50:14Z
BIOMEDICH:ART
Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening
Amin, Mohamad
Putra, Kurniawan Setia
Amin, Ihya Fakhrurizal
Earlia, Nanda
Maulina, Dina
Lukiati, Betty
Lestari, Umie
Allium cepa L.; anti inflamation; in silico; quercetin
Inflammation is a tissue injury that occurs due to physical trauma or microbiological substances that involve the activities of many cell types. Inflammation can be prevented using the natural medicines from Allium cepa L. Quercetin is one of the bioactive compounds found in Allium cepa L and has been reported to have anti-inflammatory activity. The natural medicines have been used to minimize non-steroidal anti-inflammatory drugs. This study aims to investigated the modeling structures and the protein receptor from quecertin in inflammation mechanism and their optimization of the effectiveness in the human body. The bioinformatics tools used in this study are the database of quercetin compounds, Pubchem and Swis Target Prediction protein prediction databases, PyRx 0.8 molecular docking software, ligand docking, and binding site analysis with PyMOL and LigPlus software. The results from in silico show that quercetin compounds can interact with Muscleblind-like protein 1 target protein with a Binding Affinity minus value which is not much different from the dexamethasone compound. Dexamethason is a standart because it is a corticosteroid drug that can be used as an anti-inflammatory to reduce inflammation, allergic reactions, arthritis and other inflammatory diseases.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2018-04-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/76
10.14421/biomedich.2018.71.27-31
Biology, Medicine, & Natural Product Chemistry; Vol 7, No 1 (2018); 27-31
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/76/60
Copyright (c) 2018 Mohamad Amin, Kurniawan Setia Putra, Ihya Fakhrurizal Amin, Nanda Earlia, Dina Maulina, Betty Lukiati, Umie Lestari
oai:ojs.sciencebiology.org:article/354
2024-03-20T02:04:38Z
BIOMEDICH:ART
Identification of Primary and Secondary Metabolites of Apis cerana Honey using FTIR-ATR Diamond Spectroscopy and Their Botanical Origin
Lestari, Tiffany Hanik
Susandarini, Ratna
Biology; Biochemistry; Natural Product Chemistry
Apis cerana; biochemical characterization; honey; spectroscopy analysis
Apis cerana Fab. is one of the popular honeybees species among beekeepers in Indonesia. This species is easy to care for and produces valuable honey products. Honey from A. cerana is abundantly available in traditional and modern markets in Indonesia. The purpose of this study was to identify the primary and secondary metabolites in the honey produced by A. cerana using FTIR-ATR Diamond spectroscopy. Twelve samples of honey from three provinces in Java Island were used in this study. In general, all honey samples contained protein, carbohydrate, water, alcohol, cellulose, alkaloid, tannin, and flavonoid. Variation on primary and secondary metabolites in honey samples was strongly affected by the botanical origin, geographical origin, and the local condition around beekeeping areas where the honeycombs were placed.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
none
2023-08-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/354
10.14421/biomedich.2023.122.467-475
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 467-475
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/354/217
Copyright (c) 2023 Tiffany Hanik Lestari, Ratna Susandarini
oai:ojs.sciencebiology.org:article/130
2022-01-11T09:12:17Z
BIOMEDICH:ART
Compliance Level of Textual Therapeutic Usage of Kshirakakoli Containing Formulations with a Serial Ethnomedicinal Survey and Modern System of Medicine
Kaur, Gunpreet
Gupta, Vikas
Sharma, Ravinder
Kumar, Sanjiv
Singhal, R G
Singh, Ranjit
Bansal, Parveen
Natural Products; Ayurveda; Herbal drugs
Ethnomedicinal survey; Kshirakakoli; Formulations; Ayurveda; Fritillaria roylei
Fritillaria roylei (Kshirakakoli) is a primal plant used in ancient times. But nowadays, due to biotic and abiotic stress the plant has entered in the list of threatened medicinal plant. In ancient texts effective uses of formulations containing kshirakakoli are well mentioned but the information is not written in simple language due to which the therapeutic value of the plant is not well understood by scientific fraternity. So, there is a major need to perform ethno medicinal survey for the formulations containing kshirakakoli and compare their therapeutic uses as mentioned in text with the modern system of medicine. In this study, a field survey was performed in 4 states i.e., Uttar Pradesh, Uttrakhand, Punjab and Himachal Pradesh where the conversation regarding usage of this plant or formulation was done with 24 local medical practitioners, 18 shopkeepers and 4 traditional healers. The information thus obtained were recorded and then compared. Results showed that the usage of kshirakakoli containing formulations was highest in Himachal Pradesh and Uttrakhand. Only few clinical studies have been done on these formulations. The effectiveness of the formulations against remedies alluded by the trado-medical practitioners claimed was found accurate as per ayurvedic textual literature. Hence, the ethno medicinal survey provides a precise guidance to scientists for future research on these kshirakakoli containing formulations that are useful in plethora of disorders.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/130
10.14421/biomedich.2021.101.7-14
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 1 (2021); 7-14
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/130/107
Copyright (c) 2021 Gunpreet Kaur, Vikas Gupta, Ravinder Sharma, Sanjiv Kumar, R G Singhal, Ranjit Singh, Parveen Bansal
oai:ojs.sciencebiology.org:article/205
2022-09-20T02:36:19Z
BIOMEDICH:ART
Detection of the Atherosclerotic PCSK9 gene Inhibitors Through in silico Method to Improve Targeted Therapy
Sethuramalingam, Sabarinathan
Ravi, Revathy Leena
Rajiah, Janet Rani
Biology; Biotechnology; Bioinformatics; Medicine; Chemistry
PCSK9; Therapeutics; ACE inhibitors; Protein – ligand; Docking
The PCSK9 is one of the most important marks for the evolution of therapeutic agents for atherosclerosis because its interaction with low-density lipoprotein receptors causes atherosclerosis. Protein-ligand interactions help us to understand the true mechanism of pharmacological action. This study seeks to identify the most powerful suppression options for PCSK9. Initially, the reported ACE inhibitors were included in pharmacophore modeling using PharmaGist. Next, ZINCPHARMER was used to screen the selected model against a ZINC database to identify putative drug candidates docked to the target protein to understand the interactions. The 10 best pharmacological candidates for PCSK9 with a binding energy of 9.8-8.2 kcal mol-1 were identified by molecular docking and their pharmacokinetic properties and oral bioavailability were evaluated. The (S) severalplant obtained chemicals have been discovered, including anti-hypersensitive drugs such as “Canadine, Hesperetin, and Labetalol”. According to Biochemistry, these compounds formed a stable “protein-ligand” complex. The (S) canadine PCSK9 complex had the lowest RMSD and was the most stable. Future in vitro studies could identify (S) canadin as a promising atherosclerosis inhibitor for the evolution of novel PCSK9 inhibitors.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Nothing relevant to disclose
2022-07-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/205
10.14421/biomedich.2022.112.119-131
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 2 (2022); 119-131
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/205/147
Copyright (c) 2022 Sabarinathan Sethuramalingam, Revathy Leena Ravi, Janet Rani Rajiah
oai:ojs.sciencebiology.org:article/24
2018-08-20T01:52:43Z
BIOMEDICH:ART
The Phytoestrogenic Potential of Yam Bean (Pachyrhizus erosus) on Ovarian and Uterine Tissue Structure of Premenopausal Mice
Primiani, Cicilia Novi
yam bean, isoflavone; phytoestrogen; ovarium; uterus; menopause
The use of estrogen hormone by public has significantly been improved either as prevention or treatment of disease. Menopausal issues in women are often treated using hormone replacement therapy. In regard to this, yam bean is found to contain genistein and daidzein compounds with a chemical structure that resembles estrogen hormone, therefore yam bean is categorized in the phytoestrogen group. The purpose of this study was to identify the potential of yam bean on ovarian and uterine histology of mice. This research employed a Completely Randomized Design of experimental research approach of one factor namely yam bean in three different dosage treatment: 0.3 g/kg, 0.6 g/kg, and 0.9 g/kg of yam bean for 24 days. The surgery and organ harvesting of ovary and uterus were conducted on Day 25 along with the making of histological preparat using paraffin method and Hematoxylin-eosin (HE) staining. The data was then analyzed descriptively. This research found that there were both secondary and tertiary follicle proliferation as the antrum contains some estrogen level. Meanwhile, the endometrial tissue of the uterus experienced uterine glandular proliferation. To conclude, yam bean was found to be a natural estrogen source.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/24
10.14421/biomedich.2015.41.5-9
Biology, Medicine, & Natural Product Chemistry; Vol 4, No 1 (2015); 5-9
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/24/11
Copyright (c) 2015 Cicilia Novi Primiani
oai:ojs.sciencebiology.org:article/278
2023-08-09T16:42:43Z
BIOMEDICH:ART
In-Vivo Alpha-Amylase and Alpha-Glucosidase Inhibitory Activities of Solanum anomalum Leaf Extract and Fractions
Okokon, Jude Efiom
Etuk, Idongesit Charles
Udobang, John Akpan
Ebong, Nwakaego Omonigho
Natural Product; Oxidative Stress; Toxicology
alpha amylase; alpha glucosidase; hypoglycemia; Solanum anomalum
Solanum anomalum Thonn. ex Schumach. (family Solanaceae), an edible shrub whose fruits and leaves are used medicinally to treat diseases including diabetes was evaluated for effect on alpha amylase and alpha glucosidase enzymes in vivo. The leaf extract (70-210 mg/kg) and fractions (hexane, dichloromethane, ethyl acetate, methanol, 140 mg/kg) of S. anomalum were evaluated in vivo for inhibitory effect on alpha amylase and alpha glucosidase enzymes using starch, sucrose and maltose as substrates. Acarbose was used as reference drug. The leaf extract especially middle dose (140 mg/kg) and fractions (ethyl acetate and hexane) caused significant (p<0.05) reduction in blood glucose levels of treated with the various substrates used. Ethyl acetate fraction exerted the highest inhibitory effect when starch and maltose were used as substrates followed by n-hexane and methanol. n-Hexane was the most active fraction followed by ethyl acetate when sucrose was used as substrate. The results suggest that the leaf extract and fractions of S. anomalum have the potentials to inhibit alpha amylase and glucosidase in rats.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
None
2023-01-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/278
10.14421/biomedich.2023.121.127-132
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 127-132
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/278/175
Copyright (c) 2023 Jude Efiom Okokon, Idongesit Charles Etuk, John Akpan Udobang, Nwakaego Omonigho Ebong
oai:ojs.sciencebiology.org:article/48
2018-08-20T01:50:51Z
BIOMEDICH:ART
The Female Population Growth Projection Year 2021 in Trenggalek Regency by Leslie Matrix Model on the Birth Rate and Life Expectancy
Anggreini, Dewi
Eigen value; eigenvector; leslie matrix
This research aims to determine the number of female residents in Trenggalek Regency in 2021 based on data on birth rate and life expectancy. The use of eigenvalues and eigenvectors aims to determine the dividing age distribution by Leslie matrix model. The eigenvectors are used to determine the number of female populations of each age interval, while the eigenvalues are used to determine population growth rates. The research method used is to determine the subject of research. The next stage is to collect research data, then analyze the data and last draw conclusions. The research data is obtained from BPS Kabupaten Trenggalek and BPS East Java Province that is data of woman population from year 2010-2015. The result of this research using Leslie matrix model for female population in Trenggalek Regency that is discrete model. The discrete model is divided into fourteen age intervals constructed using the birthrate and life expectancy. The conclusions of the study showed that the number of female population in Trenggalek Regency tended to increase with positive eigen value greater than one. In other words, the growth rate of female population in Trenggalek Regency tends to be positive. The success of Leslie's matrix model is the application of case studies in predicting the number of female populations in Trenggalek District by 2021 using the MAPLE 16 Program.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2017-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/48
10.14421/biomedich.2017.62.37-45
Biology, Medicine, & Natural Product Chemistry; Vol 6, No 2 (2017); 37-45
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/48/39
Copyright (c) 2017 Dewi Anggreini
oai:ojs.sciencebiology.org:article/289
2023-08-12T13:44:13Z
BIOMEDICH:ART
The Anticancer Properties of Guava Leaves (Psidium guajava L.) and Turmeric Rhizome (Curcuma longa L.) Against Breast Cancer: A Literature Study
Asiandu, Angga Puja
Sari, Widya
Sari, Septi Widiya
Majid, Alif Syahrul Abdul
Biology, Cell Biology, Phytochemistry, Medical Biology
apoptosis; curcumin; natural sources; phytochemical;
Breast cancer, one of the most deadly diseases occurring in women, is caused by factors. In the healing process, the sufferer needs treatment, such as radiation techniques, surgery, and chemotherapy. But, these techniques have avoidable weaknesses that damage healthy cells. To date, natural sources can be utilized in medicine field. Guava (Psidium guajava L.) and turmeric (Curcuma longa L.) are two common plants obtaining compounds that inhibit the growth of cancer cells based on phytochemical properties. This study was written to review the potential both of plants as anticancer agents. Through the literature, guava leaf extract consists of flavonoids, tannins, alkaloids, saponins, terpenoids that inhibit the proliferation of cancer cells. Besides, turmeric also has tannins, alkaloids, terpenoids, flavonoids, glycosides, sterols, and curcumin. The presence of curcumin reduces histamine production which induce inflammation and decrease toxin. Because of curcumin, breast cancer cells have dehydration before apoptosis.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-08-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/289
10.14421/biomedich.2023.121.407-412
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 407-412
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/289/205
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/289/40
Copyright (c) 2023 Angga Puja Asiandu, Widya Sari, Septi Widiya Sari, Alif Syahrul Abdul Majid
oai:ojs.sciencebiology.org:article/113
2021-07-21T20:50:54Z
BIOMEDICH:ART
Prevalence of Psychoactive Substance Use Among Nigerian Male Commercial Vehicle Drivers Selected from The Three Major Ethnic Groups in Nigeria
Egwuatu, Tochukwu Frank
Iroanya, Onyekachi Ogbonnaya
Adekoya, Khalid Olajide
Biological; Biology; Forensic Toxicology; Journal Biology; Medicine Journal;
Psychoactive drug; Accident; Drivers; Forensic toxicology; Nigeria
The use of psychoactive substances for several reasons by commercial vehicle drivers has been documented as a major cause of increased incidences of traffic accidents globally. The study aimed at determining the prevalence of psychoactive substance use among Nigerian male commercial vehicle drivers randomly selected from the three major ethnic groups in Nigeria (Igbo, Hausa and Yoruba). A structured questionnaire which included data on sociodemographic status and psychoactive drug use history was introduced to all participants. Generic Multi-Drug Urine Dip Card Test Kit and Shimadzu chromatograph system (Shimadzu Corporation, Kyoto, Japan) were used for toxicological analyses. Out of the 264 Nigerian male commercial vehicle drivers who participated in this study, 111(42.0 %) had used illicit drugs in recent times, 55(20.8 %) took drugs for improved energy and 90(34.1 %) felt high after using illicit drugs. Marijuana was the most abused substance; 77(29.2 %) followed by benzodiazepine; 34(12.9) across all ethnicities studied. Across all ethnicities, HPLC revealed that the Hausa drivers had the highest concentration of psychoactive substances in their urine samples. These findings, reiterates the need for the Nigerian anti-drug agency to develop new strategies in conducting a repressive drug war and extending the same to bus stations.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2020-07-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/113
10.14421/biomedich.2020.91.47-55
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 1 (2020); 47-55
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/113/98
Copyright (c) 2020 Tochukwu Frank Egwuatu, Onyekachi Ogbonnaya Iroanya, Khalid Olajide Adekoya
oai:ojs.sciencebiology.org:article/422
2024-03-20T02:04:38Z
BIOMEDICH:ART
Potency of Biocoagulant from Cationic Modified Starch of Balbis Banana Blossom Waste for Palm Oil Wastewater Treatment: Literature Study
Fadhallah, Esa Ghanim
Hidayat, Alif Fikri Nur
Saffanah, Arfika Julya
Iswahyudi, Bella Amanda
Wasalamah, Diah Shihatin
Putri, Eka Cahyani
Ningtias, Nadila
Ummi, Yana Hijriyatin
banana blossom; biocoagulant; cationic starch; POME
Lampung is one of the provinces producing Indonesia's second-largest banana crop. Operational activities at the Palm Oil Mill produce a by-product of Palm Oil Mill Effluent (POME), which can potentially be the most enormous environmental pollution. Communities often use chemicals to treat liquid waste, which causes health problems, cannot be decomposed, and can damage the environment. Therefore, treating liquid waste using organic materials that are more environmentally friendly, safe for health and easily degraded is necessary. By modifying cationic starch, natural polymers found in banana blossom waste may be utilized for producing natural biocoagulant that are more effective. This paper aims to review the potential of cationic-modified balbis banana blossom waste starch as a natural biocoagulant for processing palm oil waste. Starch was first modified into cationic starch by etherification method with the help of HMMAHC cationic reagents. The mechanism of cationic starch as a biocoagulant is the exchange of starch cation ions and waste anions to form bridges between colloidal particles and then form flocs that can precipitate. The implementation technique of this idea is by collaborating with several parties to ensure the successful use of banana hump starch as a biocoagulant material, providing support and facilities for the industry and promoting the use of biocoagulant, conducting literature studies related to the use of banana hump starch as a natural biocoagulant, testing the effectiveness of biocoagulant, implemented in the palm oil processing industry.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2024-01-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/422
10.14421/biomedich.2023.122.645-649
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 645-649
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/422/241
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/422/56
Copyright (c) 2024 Esa Ghanim Fadhallah, Alif Fikri Nur Hidayat, Arfika Julya Saffanah, Bella Amanda Iswahyudi, Diah Shihatin Wasalamah, Eka Cahyani Putri, Nadila Ningtias, Yana Hijriyatin Ummi
oai:ojs.sciencebiology.org:article/171
2022-07-31T01:51:06Z
BIOMEDICH:ART
The Bioprospecting of Mangrove Red Snapper Cultivation (Lutjanus argentimaculatus Forsskål, 1775) Using Floating Cages
Asiandu, Angga Puja
Malayudha, Achmad Gusti
Biological; Biology; Journal Biology; Aquaculture; Bioeconomics
Aquaculture; Feed; Floating Net Cages; Snapper
Mangrove red snapper is one of the potential and valuable aquaculture products. The high demand for this fish causes high fishing activities. This condition can threaten their existence in the environment. It is necessary to have aquaculture activities to preserve their existence and meet market demand. Indonesia as a country with wide marine waters should be able to take advantage of the potential of fish farming by using a floating cage system. Thus, this article was written to analyze the bioprospecting of red snapper aquaculture. Based on the literature study, the floating net system has the potential to be developed on a large scale because the system has various advantages over fishing directly from nature. Things that must be considered in the cultivation of mangrove red snapper using floating nets are the area and quality of the floating nets, the composition of the feed, and the chemical components of the feed given to the fish. Based on economic potential, mangrove red snapper cultivation has high prospects which can be used as one of Indonesia's leading export products.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/171
10.14421/biomedich.2022.111.27-33
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 1 (2022); 27-33
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/171/137
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/171/29
Copyright (c) 2022 Angga Puja Asiandu, Achmad Gusti Malayudha
oai:ojs.sciencebiology.org:article/6
2018-08-20T01:53:05Z
BIOMEDICH:ART
Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography
Ekaputri, Rahmawati
Sudarsono, Sudarsono
Mulyaningsih, Budi
larvicidal activity; Vinca rosea; Chromatography; secondary metabolites; Aedes aegypti
Background: Vinca rosea is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of Vinca rosea was selected to investigation its larvicidal activity against the 3rd instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) Aedes aegypti. Five concentrations of Vinca fruit extract were tested against the 3rd instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC50 anf LC90) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC50 and LC90 values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2014-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/6
10.14421/biomedich.2014.31.31-33
Biology, Medicine, & Natural Product Chemistry; Vol 3, No 1 (2014); 31-33
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/6/5
Copyright (c) 2014 Rahmawati Ekaputri, Sudarsono, Budi Mulyaningsih
oai:ojs.sciencebiology.org:article/39
2018-08-20T01:52:01Z
BIOMEDICH:ART
Optimization of Binocular Microscope with Micro Digital Camera for Measuring Seminiferous Tubules Epithelium Height
Sutriyono, Sutriyono
seminiferous tubules epithelium; binocular microscope; micro digital camera; mouse testis
Binocular microscope optimization using micro digital camera to measure seminiferous tubules epithelium of mouse testis (Mus muculus) was completed. This research was conducted at Biology Laboratory of Faculty of Science and Technology UIN Sunan Kalijaga. The purpose of this study was to determine the height of the seminiferous tubules epithelium of mouse testis using micro digital camera. A computer was connected with binocular microscope and optilab advance, then calibration of optilab advance were done on objective lens magnification at 4x, 10x and 40x with 10x ocular lens. The analysis used was descriptive analysis. The mean of the seminiferous tubules epithelium height is 105.6 μm. Optimization on computer and binocular microscope with micro digital camera can be used to measure seminiferous tubules epithelium.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2016-10-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/39
10.14421/biomedich.2016.52.41-47
Biology, Medicine, & Natural Product Chemistry; Vol 5, No 2 (2016); 41-47
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/39/30
Copyright (c) 2016 Sutriyono Sutriyono
oai:ojs.sciencebiology.org:article/312
2023-08-12T13:44:13Z
BIOMEDICH:ART
Synthesis, Spectroscopic Analysis and Antidiabetic Properties of Copper (II) Complex of Mangifera indica Leaf Crude Extract
Oladipo, Mary Adelaide
Ajao, Folasade Omobolanle
Adepoju, Adewusi John
Ishola, Kayode Taiwo
Ajeigbe, Olalekan Jamiu
Albino rats; Body weight; Blood glucose; Metal complex
Many applied conventional drugs in treating diabetes have been reported to possess some drawbacks which necessitate a search for alternative therapies. In order to search for a more active antidiabetic agent, this study synthesized and evaluated antidiabetic properties of Mangifera indica crude extract and its Cu (II) complex in alloxan-induced diabetic albino rats. The leaf crude extract and its metal complex were characterized using percentage metal analysis and IR spectroscopic data. Experimental animals were induced by a single intraperitoneal injection of Alloxan monohydrate at a single dose of 140 mg/kg body weight and animals with fasting blood glucose level (BGL) > 200 mg/dL were considered diabetic. Metformin was used as a standard drug. Fasting blood glucose level and body weight were used to assess the antidiabetic activity. One-way ANOVA was used to determine the level of statistically significant at p< 0.05. The crude extract was found to coordinate with the metal ion through O donor atom of C=O and O-H of phenol and ketone respectively. The Cu (II) complex of the crude extracts at tested dose of 600mg/kg demonstrated more antidiabetic activity without weight gain than the standard drug. It is concluded that the Cu (II) complex could be a potential material in the development of more active and negative-side-effect-free antidiabetic drug.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-03-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/312
10.14421/biomedich.2023.121.315-321
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 315-321
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/312/196
Copyright (c) 2023 Mary Adelaide Oladipo, Folasade Omobolanle Ajao, Adewusi John Adepoju, Kayode Taiwo Ishola, Olalekan Jamiu Ajeigbe
oai:ojs.sciencebiology.org:article/91
2020-04-11T02:51:47Z
BIOMEDICH:ART
Hepatotoxic Assessment of Tramadol-Diclofenac Use: A Study in a Rat Model
Adikwu, Elias
Nelson, Ebinyo Clemente
Medicine Journal; Medicine
tramadol; diclofenac; co-treatment; toxicity; liver; rat
The concurrent use of tramadol and diclofenac may increase hepatotoxic risk due to their individual hepatotoxic effects. This study assessed the hepatotoxic effect of tramadol-diclofenac administration in albino rats. Twenty-four adult male albino rats (200-220g) randomized into four groups were orally administered with tramadol (12mg/kg/day), diclofenac (6mg/kg/day) and tramadol-diclofenac for 14 days respectively. The rats were anesthetized, blood samples were collected and evaluated for serum liver function and lipid parameters. Liver samples were weighed and evaluated for biochemical parameters and histology. The effects of tramadol-diclofenac on the body and liver weights did not differ significantly (p>0.05) when compared to control. Also, effects were not significant (p>0.05) on blood glucose, and serum cholesterol, triglyceride, low and high density lipoprotein cholesterol levels when compared to control. Liver and serum levels of aminotransferases, alkaline phosphatase, lactate dehydrogenase, gamma–glutamyl transferase, conjugated bilirubin and total bilirubin increased significantly in rats treated with tramadol (p<0.05), diclofenac (p<0.01) and tramadol-diclofenac (p<0.001) when compared to control. Furthermore, significant decreases in liver catalase, glutathione, superoxide dismutase, glutathione peroxidase levels with significant increases in malondialdehyde levels occurred in rats treated with tramadol (p<0.05), diclofenac (p<0.01) and tramadol-diclofenac (p<0.001) when compared to control. Hepatocyte necrosis was observed in rats treated with tramadol-diclofenac. Tramadol-diclofenac may increase hepatotoxic risk at doses used for this study.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/91
10.14421/biomedich.2019.82.41-45
Biology, Medicine, & Natural Product Chemistry; Vol 8, No 2 (2019); 41-45
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/91/88
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/91/6
Copyright (c) 2019 Elias Adikwu, Ebinyo Clemente Nelson
oai:ojs.sciencebiology.org:article/399
2024-03-20T02:04:38Z
BIOMEDICH:ART
In silico Study on Structural Inhibition of Bacterial DNA Gyrase by Major Secondary Metabolites Found in Grape Seed Extract
Lawan, Haritha
Tharakee, Hashitha
Natural Product; Natural Product Chemistry; Computational chemistry
Grape seed extract; Polyphenols; catechin; molecular Docking; Pharmacokinetic; Physicochemical
In the early 20th century, during the era of investigating and identifying essential “vitamins”, scientific research focused on grape seed extracts and their bioactive components, particularly polyphenols. Extensive studies have demonstrated that grape seed extract, rich in proanthocyanidins, offers protection against a wide spectrum of diseases, encompassing inflammation, cardiac ailments, peptic ulcers, hypertension, diabetes, cancer, and microbial infections. To explore potential secondary metabolites within grape seed extract that could serve as structural inhibitors of bacterial DNA Gyrase, molecular docking studies were performed. The docking results revealed that two phytochemicals, namely (-)-catechin and Procyanidin-B2, exhibited the highest potency in inhibiting DNA gyrase subunit B. Subsequent in silico physicochemical and pharmacokinetic parameter predictions were conducted using specialized web servers for the examined phytochemicals. Notably, (-)-catechin displayed superior inhibitory and ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) characteristics, suggesting its potential for utilization in synthesizing novel antibacterial compounds.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-11-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/399
10.14421/biomedich.2023.122.585-592
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 585-592
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/399/233
Copyright (c) 2023 Haritha Lawan, Hashitha Tharakee
oai:ojs.sciencebiology.org:article/158
2022-07-31T08:19:35Z
BIOMEDICH:ART
Phytochemical Screening, Antioxidant and Antibacterial Activities of the Root Extract of Cyphostemma adenocaule (Steud. ex A. Rich.) Wild & R.B.Drumm
Yakubu, Abdulbasit Haliru
Mohammed, Mohammed Mustapha
Bababe, Abdulqadir Bukar
Braimah, Hassan Yesufu
Natural Product Chemistry; Phytochemistry; Medicinal Chemistry
Cyphostemma adenocaule; Phytochemical screening; antioxidant and antibacterial activity
Plant secondary metabolites have provided important bioactive principles for developing new lead compounds. Within their confinement, they exhibit unique chemical diversity, which influences their diverse biological properties. The Vitaceae family is known for its potent antioxidant and antibacterial phytoconstituents, among other biological properties. Cyphostemma adenocaule is one of the family members explored for its ethnomedicinal properties. This study undertook the evaluation of the phytochemical, antioxidant, and antibacterial properties of the root extract of Cyphostemma adenocaule. Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, carbohydrates & glycoside, saponins, and tannins. The methanol root extract had the highest activity in the DPPH assay, providing IC50 (50% inhibition) of 10.87µg/ml, followed by n-Hexane (IC50 74.10µg/ml) and chloroform (IC50 74.31µg/ml) extract. In the antibacterial assay, the chloroform extract was active against E. coli (24.00±0.15) and had moderate activity against Staph. aureus (12.5±0.18). The n-Hexane extract was completely inactive against the test organisms while the methanol extract showed poor activity against the test organisms. The present study adds to the existing literature on Cyphostemma adenocaule with scientific evidence into its biological properties.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-10-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/158
10.14421/biomedich.2021.102.105-110
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 2 (2021); 105-110
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/158/127
Copyright (c) 2021 Abdulbasit Haliru Yakubu, Mohammed Mustapha Mohammed, Abdulqadir Bukar Bababe, Hassan Yesufu Braimah
oai:ojs.sciencebiology.org:article/267
2023-08-09T16:42:43Z
BIOMEDICH:ART
The Association Between Some Endocrine Conditions and COVID-19: A Review
Smail, Harem Othman
COVID-19; diabetes mellitus; bidirectional; obesity; pathophysiology; blood pressure; hypothyroidism
The review aimed to understand and explain the association between some major endocrine conditions and COVID-19. Since March 2019, COVID-19 has been identified as a pandemic by the World Health Organization (WHO) and has infected millions of individuals worldwide. According to the literature review, endocrine disorders include diabetes, obesity, hypertension, and thyroid. The development and progress of COVID-19 patients could be affected but not yet approved by all the studies. In diabetes mellitus, COVID-19 may affect cytokines and increase releases of IL-1, IL-6, and complications caused by diabetes. In obese patients, there was an increased risk of progressing to severe COVID-19. Because of the international spread of severe acute respiratory syndrome coronavirus, clinicians should pay special attention to obese patients who should be monitored closely with timely and aggressive care. COVID-19 pathophysiology and risk in a high incidence of hypertension has been found in patients with COVID-19 and can be studied in many kinds of studies, such as in China. However, hypertension is considered one of the most significant risk factors for COVID-19. In addition to the above data on associations between COVID-19 and endocrine disorders, data on thyroid function or thyroid disease in COVID-19 is not yet available and cannot be commonly reported.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-10-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/267
10.14421/biomedich.2023.121.45-53
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 45-53
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/267/166
Copyright (c) 2022 Harem Othman Smail
oai:ojs.sciencebiology.org:article/29
2018-08-20T01:52:35Z
BIOMEDICH:ART
Antioxidant Potential of Black, Green and Oolong Tea Methanol Extracts
Widowati, Wahyu
Herlina, Tati
Ratnawati, Hana
Constantia, Gabriella
Deva, I Dewa Gde Sathya
Maesaroh, Maesaroh
antioxidant; black tea extract; green tea extract; oolong tea extract; total phenolic content
Degenerative diseasesandchronicdiseasesare oftencausedbyoxidativestress. Oxidative stress caused by free radicals. Antixodant as inhibitor are needed to prevent it which is one of antioxidant sources is tea. Tea processinggenerally produce various kinds of teas such as black, green and oolong tea. Tea processing affect thecontent ofphenolic compounds. The aim of the research is to evaluate phytochemical content, totalphenolic content of blacktea, green tea andoolongtea extracts using catechin, quercetin, kaempferol, myricetin as standard, and to evaluate the antioxidative potency of black tea, green tea and oolong tea extracts compared to catechin, quercetin, kaempferol, myricetin. Phytochemical assay using modified Farnsworth method, the antioxidant activity were measured by by its 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity. Green tea extract contained highest phenolic and flavonoid. The highest antioxidant activity was green tea extract with IC50=0,487μg/mL. Green tea extract content phenol and flavonoid are higher compared to the other extracts, green tea extract has the highest antioxidant activity.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-10-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/29
10.14421/biomedich.2015.42.35-39
Biology, Medicine, & Natural Product Chemistry; Vol 4, No 2 (2015); 35-39
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/29/17
Copyright (c) 2015 Wahyu Widowati, Tati Herlina, Hana Ratnawati, Gabriella Constantia, I Dewa Gde Sathya Deva, Maesaroh Maesaroh
oai:ojs.sciencebiology.org:article/319
2023-08-09T16:42:43Z
BIOMEDICH:ART
Synthesis and Characterization of Cinnamon Loaded BSA Microparticles with Antidiabetic Properties
Wanniarachchi, Binuki
Sathsarani, H.M.W.K.
Jayawardena, Bimali M.
Dewangani, H.G.N.
Biological; Biology; Journal Biology; Medicine Journal; Medicine; Natural Product; Natural Product Chemistry; Journal Natural Product
Cinnamon; Cross-linking agent; Diabetes; Microparticles; Nutraceutical
Conventional medicine that is being used to treat diabetes exert adverse side effects and therefore scientists have focused on natural hypoglycemic agents. Sri Wijaya (CCSW) is an accession of Cinnamomum zeylanicum, which shows higher hypoglycemic activity. Pressured water extract of its dried quills can be used as an antidiabetic nutraceutical. Higher stability, ease of storage and transportation, make powder form nutraceuticals more preferred. The objective of this study was to develop cinnamon encapsulated microparticles as a powder form nutraceutical with higher hypoglycemic activity. Four different products were synthesized. Two of them were synthesized using bovine serum albumin (BSA) (8.8 % (w/v), pH=5) in the presence of citric acid and ascorbic acid as cross-linking agents separately. The other products were synthesized using BSA (20 mg/ mL, pH=9) in the presence of same cross-linking agents. Antidiabetic activity of the products was determined using alpha-amylase and alpha glucosidase inhibition assays and compared with that of crude cinnamon extract and positive control acarbose. Since the product synthesized using BSA (20 mg/ mL, pH=9) and citric acid showed the highest alpha amylase inhibition activity, solubility, cinnamon loading percentage and cinnamon entrapment efficiency those conditions were concluded as the optimum conditions required to synthesize microparticles with higher hypoglycemic activity. Particle size, polydispersity index, and zeta potential of that product were 1.281 (0.004) m, 0.460 (0.018) and -1.09 (0.03) mV respectively. According to the SEM image, microparticles have a spherical morphology. The UV-visible spectrum and the FT-IR spectrum confirm the entrapment of cinnamon compounds.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
University Grant RP/03/02/06/01/2021
2023-03-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/319
10.14421/biomedich.2023.121.241-250
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 241-250
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/319/187
Copyright (c) 2023 Binuki Wanniarachchi, H.M.W.K. Sathsarani, Bimali M. Jayawardena, H.G.N. Dewangani
oai:ojs.sciencebiology.org:article/82
2020-04-11T03:13:43Z
BIOMEDICH:ART
Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)
Rakhmiyati, Rakhmiyati
Luthfi, Muhammad Ja’far
Common house gecko (Hemidactylus frenatus); Autotomy; Alizarin Red- S Alcian Blue; cartilage; bone (osteon)
Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2018-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/82
10.14421/biomedich.2018.72.57-59
Biology, Medicine, & Natural Product Chemistry; Vol 7, No 2 (2018); 57-59
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/82/70
Copyright (c) 2018 Rakhmiyati, Muhammad Ja’far Luthfi
oai:ojs.sciencebiology.org:article/353
2024-03-20T02:04:38Z
BIOMEDICH:ART
Targeting H3N2 Influenza Virus RNA-dependent RNA Polymerase by Using Bioactives from Essential Oils from Eucalyptus polybrachtea, Cymbopogon citratus and Cymbopogon khasianus
Sharma, Arun Dev
Kaur, Inderjeet
Docking; Eucalyptus oil; Lemon grass oil; Palmarosa oil; Herbal Drug
A dramatic surge of H3N2 influenza virus is of grave concern worldwide and particularly in India. H3N2 cause acute respiratory infection, however, a few drugs are available for its mitigation. Subsequently, researchers have been involved in efforts to discover novel antiviral mechanisms that can lay the basis for new anti-influenza drugs. Influenza virus RNA-dependent RNA polymerase (RdRP) is a multi-functional hetero-trimer, implicated in the production of viral mRNA, hence plays a major role in viral infectivity thus directly associated with survival of the virus. RdRP have been cited as anappropriate target for therapeutic drug design. In the present study molecular docking was designed to estimate the effect of potent bioactive moleculesfrom essential oils from Eucalyptus polybrachtea (eucalyptus oil, EO), Cymbopogon citratus (lemon grass essential oil, LEO) and Cymbopogon khasianus (palmarosa essential oil, PEO) against RdRP protein. GC-FID (gas chromatography with flame-ionization detection) based composition profile, and in-silico docking study was conducted by using CB-dock 2 analysis followed by 2D interactions. GC-FID revealed eucalyptol, geranial and geraniolas major phytocompounds in EO, LEO and PEO respectively. The docking score indicated effective binding of ligands to RdRP. Interactions results indicated that, RdRP/ligand complexes form hydrogen, van der waals forces, pi-alkyl, alkyl, and pi-Sigma interactions. Based on above findings of aroma profile and docking, therefore, it was recommended that essential oils from above mentioned aromatic cropsmay represent potential herbal treatment to mitigate H3N2 infections.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-09-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/353
10.14421/biomedich.2023.122.515-524
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 515-524
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/353/223
Copyright (c) 2023 Arun Dev Sharma, Inderjeet Kaur
oai:ojs.sciencebiology.org:article/147
2022-01-11T09:12:17Z
BIOMEDICH:ART
Ethnobotanical Study of Edible Plant Communities on the Slopes of Mount Merapi and Merbabu, Indonesia
Umartani, Lita Ayu
Nahdi, Maizer Said
Culture; Ethnobotany; in-depth interviews; Rice (Oryza sativa L.); Mount Merapi; Mount Merbabu
Ethnobotany is a study of the interaction between local people and their natural environment, especially regarding the use of plants as food and medicinal ingredients. Edible plants are a daily basic need whose existence is a necessity for the people on the slopes of Mount Merapi and Merbabu. How to use plants is transferred from generation to generation to form a culture. The research was carried out in March-May 2020 with the aim of digging local knowledge about plants used as food, including staple food, vegetables and medicines by studying the species diversity, including the benefits of plant parts, habit, how to use, process and how to obtain these species. The data were collected by using a combination of qualitative and quantitative methods with in-depth interviews through 40 respondents who were selected by purposive sampling. The results showed that the communities on the slopes of Mount Merapi and Merbabu used 74 plant species from 37 families as food sources. The favorite family is Fabaceae, followed by Zingiberaceae and Solanaceae. The most widely used habitus of plants were herbs (36.49%), followed by bush (28.38%), shrubs (18.92%) and trees (16.22%). Plant parts that are widely used are leaves (29.73%), fruit (17.57%), tubers (10.81%), seeds (9.46%), roots, rhizomes and flowers (6.76%), shoots (5.40%), stems (2.70%) and water, skin and heartwood (1.35%). The most common ways to use it are eaten raw as vegetables (29.73%), boiled (16.22%) and drink (12.16%). How to obtain it are through own cultivation (72.97%), and buying at the market (21.62%). The highest use values were Adas (Foeniculum vulgare Mill.) (0.25), chili pepper (Capsicum annum L.) (0.20), turmeric (Curcuma dosmetica loir) and water spinach (Ipomoea aquatica Forsk.) (0.17). The highest importance values were rice (Oryza sativa L.) (5.23%), and fennel (Foeniculum vulgare Mill.) (4.57%).
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-07-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/147
10.14421/biomedich.2021.101.33-39
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 1 (2021); 33-39
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/147/112
Copyright (c) 2021 Lita Ayu Umartani, Maizer Said Nahdi
oai:ojs.sciencebiology.org:article/209
2022-09-20T02:36:50Z
BIOMEDICH:ART
Chemical Compositions and Antioxidant Activity of Volatile Oils from Morinda citrifolia and Beta vulgaris Leaves from Nigeria
Onanuga, Adesegun Olusimba
Okpala, Ejike Onwudiegwu
Natural Product; Natural Product Chemistry
Morinda citrifolia L.; Beta Vulgaris L.; Antioxidant; free radical scavenging activity volatile oil
Morinda citrifolia L. and Beta Vulgaris L leaves are both ethnomedicinal use for the treatment of arthritis, indigestion and skin infections with no reports on their essential oils compositions. The colourless volatile oils with a percentage yield of 0.6 and 0.4 (w/w) for Morinda citrifolia L. and Beta Vulgaris L respectively were obtained. Forty-five compounds representing 94.31 % of the total percentage compositions were identified in the leaf essential oil of M. citrifolia with the most abundant compound as 14-beta-H-pregna- (33.13%). Forty-eight compounds representing 74.18% of the total oil composition were identified in the leaf oil of B. Vulgaris with phytol (24.20%) as the dominant compound. The essential oils showed good free radical scavenging activity when compared to ascorbic acid used as control, with % inhibition varying from 88.74 ± 0.010 to 96.61 ± 0.004 as compared to 95.68 ± 0.010 to 97.31±0.003 of the ascorbic acid at (100 to 6.25 mg/ml) concentrations. The leaves essential oils of Morinda citrifolia L. and Beta Vulgaris L contains chemical compounds that might be responsible for their antioxidant activity. This result validates the traditional usage of these plants in the treatment of arthritis, indigestion and skin infections.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
None
2022-08-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/209
10.14421/biomedich.2022.112.161-167
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 2 (2022); 161-167
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/209/152
Copyright (c) 2022 Adesegun Olusimba Onanuga, Ejike Onwudiegwu Okpala
oai:ojs.sciencebiology.org:article/288
2023-08-09T16:42:43Z
BIOMEDICH:ART
Lupenone Isolated from Diospyros melanoxylon Bark Non-competitively Inhibits alpha-amylase Activity
Durgam, Mohan Krishna
Vemuri, Praveen Kumar
Bodiga, Vijaya Lakshmi
Bodiga, Sreedhar
Diospyros melanoxylon; Ebenaceae; Triterpenoid ketone; pancreatic amylase; non-competitive inhibition
Diabetes mellitus is a chronic disease that poses a serious global health problem, due to its associated effects on obesity and aging. Therapeutic strategies for targeting diabetes include the downregulation and/or inhibition of enzymes such as a-amylase and a-glucosidase, hydrolyzing the dietary carbohydrates in intestine. There is increasing interest for a-amylase inhibitors from natural sources. Our objective was to undertake the phytochemical screening of bark extracts of Diospyros melanoxylon for potential a-amylase inhibitory activity and further identification of the active principle and the underlying mechanisms of inhibition. Enzyme-assay guided fractionation of the Diospyros melanoxylon bark extract led to the isolation of a triterpene, Lupenone as a potential inhibitor of a-amylase, with a non-competitive inhibition and inhibitor constant = 30 mM. Lupenone-mediated inhibition of a-amylase responsible for the breakdown of dietary sugar may be effective in preventing postprandial hyperglycemia in the diabetic subjects.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
None
2023-01-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/288
10.14421/biomedich.2023.121.171-176
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 171-176
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/288/178
Copyright (c) 2023 Mohan Krishna Durgam, Praveen Kumar Vemuri, Vijaya Lakshmi Bodiga, Sreedhar Bodiga
oai:ojs.sciencebiology.org:article/70
2018-08-20T01:50:14Z
BIOMEDICH:ART
Leukocytes Description of Mudskipper (Periophthalmodon schlosseri) of Barito River Estuary, Desa Tanipah, Kalimantan Selatan
Santoso, Heri Budi
Hidayaturrahmah, Hidayaturrahmah
Muhamat, Muhamat
leukocytes’ differentiation; Desa Tanipah; Periathalmodon schlossery; mudskipper
Leukocytes have an important role in driving away infections from pathogen microorganism by phagocytosis together with macrophages. The aim of this research was to analyze the leukocytes’ differentiation (eosinophil, basophil, neutrophil, lymphocytes, and monocytes) of mudskipper‘s blood (Periophthalmodon schlosseri). Samples were taken from desa Tanipah Kalimantan Selatan. The sampling locations were decided by purposive or taking the data intentionally according to the consideration of mudskipper‘s location which is not homogenous. The data collection were conducted using Line Transect which means the sample was taken according to how many encounters can be taken. There were 15 mudskippers taken from Desa Tanipah used in this research. The parameter observed wasthe differentiation of leukocytes which consists of the percentages of monocytes, lymphocytes, eosinophil, basophil, and heterophil. The result showed that the percentage of lymphocytes in mudskippers is 62+4,1% and monocytes 24,7+0,8%, and the neutrophil is 0,5+0,1% and eosinophil 0,6+0,1% and no basophils were found. According to the result, it can be concluded that lymphocytesis the most dominant one amongst others.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2018-04-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/70
10.14421/biomedich.2018.71.1-4
Biology, Medicine, & Natural Product Chemistry; Vol 7, No 1 (2018); 1-4
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/70/61
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/70/3
Copyright (c) 2018 Heri Budi Santoso, Hidayaturrahmah Hidayaturrahmah, Muhamat Muhamat
oai:ojs.sciencebiology.org:article/396
2024-03-20T02:04:38Z
BIOMEDICH:ART
Alkaloids Lead to Potential Inhibition of the Acyl Carrier Protein Reductase to Attenuate Tuberculosis; an in-silico Analysis
Kamran, Pernia
Ibrahim, Ahsan
Natural Product Chemistry
Tuberculosis; alkaloids; ACP reductase; molecular docking analysis; Mycobacterium tuberculosis
Tuberculosis (TB) is a contagious infection that mostly affects the lungs. Mycobacterium tuberculosis causes tuberculosis infection, leading to granulomatous lesions in affected lung tissue. It is one of the most prevalent and deadly infectious diseases among the under developed countries. This study aims to investigate the possible inhibition of the acyl carrier protein reductase for preventing tuberculosis by well-known alkaloids, thereby reducing Mycobacterium tuberculosis growth in the lungs and thereby reducing the incidence of latent and active TB. About five natural alkaloids were subjected to the molecular docking analysis, which produced favorable findings in terms of best pose and binding energies of these compounds towards the active residues of mycobacterial ACP reductase, with values ranging from -10 kcal/mol to -9.1 kcal/mol. The molecular dynamics simulation produced similar encouraging results. All of the prospective alkaloid compounds were subjected to an in-silico toxicity investigation, which determined that every compound was safe and non-toxic. Further studies may be necessary for effective formulation development employing these compounds as part of the process of drug discovery and development. The findings from this study may be helpful in the development of the novel nanoformulations using natural products for pharmacotherapy of tuberculosis infection.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-08-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/396
10.14421/biomedich.2023.122.441-450
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 441-450
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/396/211
Copyright (c) 2023 Pernia Kamran, Ahsan Ibrahim
oai:ojs.sciencebiology.org:article/111
2021-07-22T06:52:01Z
BIOMEDICH:ART
A Chemical Overview of Azanza garckeana
Bioltif, Yilni Edward
Edward, Naanma Bioltif
Tyeng, Terry Dalyop
Azanza garckeana; Chemical; Compounds; Mansonone
Azanza garckeana is a popular fruit tree in Nigeria, specifically in Gombe State, where it is locally called ‘goron tula’ which means ‘Kola of Tula’. It is also found in part of some African countries. Different plant part of this small tree/shrub has recorded different uses by the locals; uses ranging from its fruits being edible and others parts helping to remedy different diseases, especially sexually related diseases. It also records use as booster for sexual performance. The uses of the plant are majorly attributed to the presence of chemicals. Its local use initiates the necessity of this review to enhance the research for drug discovery since Chemicals are the chief constituencies responsible for its medicinal importance.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2020-11-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/111
10.14421/biomedich.2020.92.91-95
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 2 (2020); 91-95
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/111/103
Copyright (c) 2020 Yilni Edward Bioltif, Naanma Bioltif Edward, Terry Dalyop Tyeng
oai:ojs.sciencebiology.org:article/438
2024-03-20T02:04:38Z
BIOMEDICH:ART
In vivo Alpha-amylase and Alpha-glucosidase Inhibitory Potentials of Panicum maximum Jacq. (Guinea grass) Leaf Extract on Wister Rats
Enin, Godwin Ndarake
Okokon, Jude Efiom
David, Enobong Mfon
Emmanuel, Saviour Elisha
Ekanem, Esther Michael
Antia, Bassey Sunday
Chemistry; Natural Product; Pharmacology; Biology; Medicine
Panicum maximum; alpha amylase; alpha glucosidase; phytochemicals
Panicum maximum Jacq. (Guinea grass) a medicinal plant used traditionally in the treatment of diseases including diabetes was evaluated for its effect on alpha amylase and alpha glucosidase enzymes in vivo. The crude ethanol extracts (150, 300 and 450 mg/kg) of P. maximum were investigated using starch, sucrose, glucose and maltose as substrates and acarbose as reference drug. The leaf extract caused significant (p<0.05) reduction in blood glucose levels of the treated rats with the four substrates used. The findings show that the leaf extract of Panicum maximum has the potentials to inhibit alpha amylase and alpha glucosidase in rats.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2024-03-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/438
10.14421/biomedich.2023.122.681-685
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 681-685
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/438/249
Copyright (c) 2024 Godwin Ndarake Enin, Jude Efiom Okokon, Enobong Mfon David, Saviour Elisha Emmanuel, Esther Michael Ekanem, Bassey Sunday Antia
oai:ojs.sciencebiology.org:article/182
2023-03-09T02:45:02Z
BIOMEDICH:ART
Phytochemical Constituents of F. Sagittifolia Warburg ex Mildbraed & Burret Leaves with Antimicrobial Activity
Taiwo, Olayombo Margaret
Olaoluwa, Olaoluwa Omosalewa
Aiyelaagbe, Olapeju Oluyemisi
Matasyoh, Josphat Clement
Natural Product Chemistry;Medicinal Chemistry;Organic Chemistry
Antimicrobial activity; F. sagittifolia; Isoflavonoids; Natural products; Steroidal glycoside
The leaves and bark of Ficus sagittifolia have been used as a cure for stomach and pulmonary disorders, respectively. The bark is edible and is taken against colic. From the leaves of F. sagittifolia, a steroidal glycoside named Stigmast-5,22-diene-3-O-?-D-glucopyranoside 1 and three isoflavonoids named 5-hydroxy-3-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one 2, 5-hydroxy-3(4-hydroxylphenyl)-8,8-dimethylpyrano[2,3-f]-chromen-4(8H)-one 3 and 5-hydroxy-3-(4-hydroxyphemyl)-8,8-dimethylpyrano[3,2-g}-chromen-4(8H)-one 4 were isolated, and this is the first report of the isolation of these compounds from this plant. The structural elucidation of the compounds was based on 1D and 2D NMR, IR and MS data analyses. Compounds 1 and 2 inhibited the growth of Pseudomonas aeruginosa and Aspergillus Niger at 0.0625 mg/mL, respectively while compounds 2 and 4 were active against Helicobacter pylori at 0.0625 mg/mL. These findings corroborate the ethno-medicinal use of F. sagittifolia leaves as a treatment for stomach disorders.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
African German Network of Excellence in Science (AGNES)
2022-06-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/182
10.14421/biomedich.2022.111.75-81
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 1 (2022); 75-81
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/182/142
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/182/31
Copyright (c) 2022 Olayombo Margaret Taiwo, Olaoluwa Omosalewa Olaoluwa, Olapeju Oluyemisi Aiyelaagbe, Josphat Clement Matasyoh
oai:ojs.sciencebiology.org:article/20
2018-08-20T01:52:56Z
BIOMEDICH:ART
Larvicidal Activity of A Mixture of Cashew Nut Shell Liquid and Water-Soluble Extract of Soap Nut Fruit (Sapindus rarak DC.) Against 3rd Instar Larvae of Aedes aegypti
Raraswati, Glory Resia
Sudarsono, Sudarsono
Mulyaningsih, Budi
Larvacidal activity; Thin Layer Chromatography; Sapindus rarak DC
Cashew Nut Shell Liquid (CNSL) which has been known as a waste of processing cashew fruits which is contain phenolic compounds have activity as larvicides. Cashew nut shell liquid is not soluble in the water where the larvae grow. Cashew nut shell liquid mixed with water-soluble extract of soapnut fruit which serves as a natural surfactant that can emulsify oil in water. The test subjects were larvae of Aedes aegypti third instar. Test subjects were divided into treatment group and control group. In the treatment group, test subjects were the mixture of CNSL and Ethylacetat Soluble Extract (ESE) in tap water. The Larvae mortality observations were done 24 hours after the treatment. LC50 and LC90 as final test data were analyzed using probit analysis. Extract constituents of CNSL and Water Soluble Extract of SoapNut fruit (WSEoSN) were investigated using Thin Layer Chromatography (TLC) method. The effect of CNSL as larvicides against third instar larvae of Ae. aegypti with were LC50 of 14,12 ppm, while the LC90 of 24,85 ppm.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-10-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/20
10.14421/biomedich.2014.32.53-57
Biology, Medicine, & Natural Product Chemistry; Vol 3, No 2 (2014); 53-57
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/20/8
Copyright (c) 2014 Glory Resia Raraswati, Sudarsono, Budi Mulyaningsih
oai:ojs.sciencebiology.org:article/37
2018-08-20T01:51:45Z
BIOMEDICH:ART
Analysis of Bull Sperm DNA Abnormalities Due to Cadmium Accumulation
Fitriawan, Fuad
Biology
Abnormality; Spermatozoa; Cow; PCR-RAPD; cadmium accumulation
Sperm abnormalities can occur by various causes. Abnormality of sperm is usually characterized by abnormal sperm motility and viability. This was caused by the inability of mitochondria on ATP-ase in producing ATP and ecto-enzyme Cik role in keeping the movement so that movement of sperm motility declines. Research that leads to total abnormal sperm DNA analysis is still rare. The purpose of this research was to get the results of the molecular characteristic picture of the overall characteristics of DNA loci that have abnormalities in bull sperm and get a picture of differences in overall DNA loci of abnormal and normal sperm. This research was conducted in October-December 2016. With the results of Group I consists of D, E with a percent similarity of 92.308%, group II consists of C and Group I with a percentage similarity of 50.125%, group II consists of A and B with a percent similarity of 100%, group II and group III with a percentage similarity of 0%. Based on the above data it can be concluded that the treatment A and B is not suspected to cause DNA damage compared to treatment C, D and E.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2017-04-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/37
10.14421/biomedich.2017.61.5-8
Biology, Medicine, & Natural Product Chemistry; Vol 6, No 1 (2017); 5-8
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/37/35
Copyright (c) 2017 Fuad Fitriawan
oai:ojs.sciencebiology.org:article/276
2023-08-12T13:44:13Z
BIOMEDICH:ART
Fiber Concentration on Fermentation of Cleome Gynandra L Based on Storage Time and Solvent Change
Restusari, Lily
Dewi, Ayu Komala
Elisanti, Alinea Dwi
Biological;journal natural product
Cleoma gynandra L; joruk maman; fiber; storage; solvent change
Cleome gynandra L (CGL) or Maman plant, is the basic ingredient of Malay food in Riau Province, Indonesia. The young leaves and stems of CGL are processed into fermented food (Joruk Maman). It contains crude fiber and is useful for lowering blood cholesterol levels. However, effective storage of this CGL has not been carried out. This study wants to see the effect of storage time and solvent change on the fiber content of Joruk Maman. An experimental study applied a completely randomized design (CRD) using 5 groups and 2 repetitions. This sample of CGL leaves was taken from one seller in the Rokan Hilir market of Riau Province. The primary outcome was a difference to the number of fibers in Joruk Maman without solvent change (p =0.001) and with solvent change (p = 0.001) based on the day group and there was no difference base on the temperature group. Secondary outcome was the difference in duration time to produce the highest fiber content at room temperature with the solvent change and not. The highest fiber content occurred at 5 days of storage at room temperature without solvent changing. Meanwhile, by changing the solvent, the fiber content would be optimal for 1-day of storage.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
DIPA funds Poltekkes Kemenkes Riau 2020
2023-06-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/276
10.14421/biomedich.2023.121.363-369
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 363-369
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/276/188
Copyright (c) 2023 Lily Restusari, Ayu Komala Dewi, Alinea Dwi Elisanti
oai:ojs.sciencebiology.org:article/106
2021-07-21T20:50:54Z
BIOMEDICH:ART
Analysis of Body Posture using Rapid Entire Body Assessment (REBA) and Rapid Upper Limb Assessment (RULA) to Improve the Posture of Sand Paper Machine Operators and Reduce the Risk of Low Back Pain
Kusuma, Trio Yonathan Teja
Rapid Eentire Body Assessment; Rapid Upper Limb Assessment; Low Back Pain
The metal casting industry, Nitikan is a micro business whose production process is carried out manually. The equipment used is simple and without considering the user's health. This can be seen in the product finishing process that uses a sanding machine. The equipment is not designed according to the user's posture so the operator bends in doing work movements, of course this is very risky to experience lower back pain injuries. This study aims to analyze the work activities of the finishing process using the Rapid Entire Body Assessment (REBA) and Rapid Upper Limb Assessment (RULA) approach and design work tools that can be applied to improve work posture. The results of data processing and analysis obtained a Rapid Entire Body Assessment score on a sandpaper machine single head of 5 and a Rapid Upper Limb Assessment score of 4, the score indicates that the work position has the potential to experience a risk of low back pain, so there must be an improvement in work posture. Work posture improvement is done by designing a tool in the form of a sandpaper machine that takes into account ergonomic dimensions to reduce the risk of lower back injury. The final result after the improvement is obtained Rapid Entire Body Assessment score of 3 and Rapid Upper Limb Assessment score of 4. This shows that the risk of lower back injuries can be minimized.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2020-04-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/106
10.14421/biomedich.2020.91.21-25
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 1 (2020); 21-25
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/106/95
Copyright (c) 2020 Trio Yonathan Teja Kusuma
oai:ojs.sciencebiology.org:article/410
2024-03-20T02:04:38Z
BIOMEDICH:ART
Investigating the Insecticidal Properties of Alangium salviifolium Root Extracts on Culex quinquefasciatus Mosquitoe
Yagoo, Alex
Milton, M.C. John
Vilvest, Jelin
Alangium salviifolium roots; Culex quinquefasciatus; plant extract; toxicity
This study evaluated the effectiveness of successive hexane, chloroform, and methanol extracts of Alangium salviifolium roots in combating mosquito-borne diseases caused by Culex quinquefasciatus mosquitoe in India. The hexane extract showed the highest efficacy with LC50 values of 105.0 ppm (larvicidal activity), 108.0 ppm (pupicidal activity), and 65.6 ppm (ovicidal activity). The chloroform extract had LC50 values of 156.8 ppm (larvicidal), 376.0 ppm (pupicidal), and 20.0 ppm (ovicidal), while the methanol extract had LC50 values of 235.7 ppm (larvicidal), 441.8 ppm (pupicidal), and 30.4 ppm (ovicidal). The hexane extract of A. salviifolium roots shows potential as a mosquito control agent, specifically against Cx. quinquefasciatus mosquitoes. Further studies are needed to assess the safety and effectiveness of this extract. This research highlights the potential of plant extracts as alternative mosquito control agents for mitigating mosquito-borne diseases in India.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-12-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/410
10.14421/biomedich.2023.122.619-624
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 619-624
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/410/237
Copyright (c) 2023 Alex Yagoo, M.C. John Milton, Jelin Vilvest
oai:ojs.sciencebiology.org:article/166
2022-07-31T08:19:35Z
BIOMEDICH:ART
Repurposing Dihydroartemisinin-Piperaquine-Doxycycline as an Antimalarial Drug: A Study in Plasmodium berghei-Infected Mice
Georgewill, Udeme Owunari
Adikwu, Elias
artemisinins; doxycycline; malaria; repurposing; resistance
Artemisinin-based combination (ACT) therapy is the mainstay for malaria treatment. However, Plasmodium parasite with decreased susceptibility to ACT has emerged. Hence, it is imperative to discover new drugs or explore new drug combinations that can decrease Plasmodium parasite resistance. This study assessed the antiplasmodial activity of dihydroartemisinin-piperaquine- doxycycline (D-P-DX) on mice infected with Plasmodium berghei. Swiss albino mice (25-30g) of both sexes inoculated with 1x107 Plasmodium berghei intraperitoneally were used. The mice were randomly grouped and orally treated with DX (2.2 mg/kg), D-P (1.71/13.7 mg/kg) and D-P-DX daily in curative, suppressive and prophylactic studies. The negative and the positive controls were treated daily with normal saline (0.2mL) and chloroquine (CQ) (10mg/kg), respectively. After treatment, blood samples were assessed for percentage parasitemia, hematological and lipid parameters. Also, the mice were observed for mean survival time. D-P, DX, and D-P-DX produced significant decreases in percentage parasitemia at p<0.05, p<0.01 and p<0.001, respectively when compared to negative control. In the curative study, D-P, DX, and D-P-DX produced 64.9%, 71.1%, and 93.6% parasitemia inhibitions when compared to 70.0% inhibition produced by CQ. Plasmodium berghei -induced alterations in packed cell volume, white blood cells, red blood cells, hemoglobin, high-density lipoprotein cholesterol, total cholesterol, low-density lipoprotein cholesterol, and triglyceride levels were significantly restored by DX (p<0.05) and D-P (p<0.01) and D-P-DX (p<0.001) when compared to the negative control. D-P-DX showed significant antiplasmodial activity against Plasmodium berghei- infected mice. It may be clinically useful for the treatment of malaria.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/166
10.14421/biomedich.2021.102.135-140
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 2 (2021); 135-140
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/166/132
Copyright (c) 2021 Udeme Owunari Georgewill, Elias Adikwu
oai:ojs.sciencebiology.org:article/273
2023-08-09T16:42:43Z
BIOMEDICH:ART
Cardiotoxicity Effects of Herbal Medicine, A Review Article
Gavanji, Shahin
Natural Product
Cardiotoxicity; heart damage; herbal medicine; toxicity; adverse effects
With the development of human's modern society more and more people tend to use complementary and alternative medicine (CAM). Toxicological studies indicated that many herbal medicines have direct toxic effects on the circulatory system or cardiovascular system and cause harmful effects on the body. Cardiotoxicity or heart damage is a serious issue defined as heart electrophysiology dysfunction, affecting the cardiac structure, and muscle damage that arises from the drug or chemical poisoning agents, that may lead to heart failure. The aim of this review article is to provide various information about the potential adverse effects of herbal medicine on the cardiovascular system and introduce herbs that induced cardiac toxicity. To provide this review, all reported cases of cardiac toxicity induced by herbal medicines and natural products were collected through research articles and documents, and the most relevant articles, and books in various authentic search engines including Scopus, PubMed, SID (scientific information database), Science Direct and Google Scholar, from 1984 to April 2022 were searched, and selected herbs with therapeutic properties which induce toxic effects on the cardiovascular system are introduced. In this review, scientific data regarding cardiotoxicity showed that 16 herbs from 11 families may increase cardiac toxicity. Therefore, it's important to use herbal medicines and natural products under the guidance of medical professionals.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-12-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/273
10.14421/biomedich.2023.121.89-96
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 89-96
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/273/169
Copyright (c) 2022 Shahin Gavanji
oai:ojs.sciencebiology.org:article/34
2018-08-20T01:52:19Z
BIOMEDICH:ART
Determination of Leisure Levels of Village Patronage UIN Sunan Kalijaga Yogyakarta: Improving Governance Patronage towards Rural Green Village and Environmentally Friendly
Supriatna, Supriatna
Niyartama, Thaqibul Fikri
Kuswidi, Iwan
Mathematical
Patronage village; Comfort Level; Temperature Humidity Index (THI)
This study took place in the village of Patronage UIN Sunan Kalidjaga Yogyakarta that consist of 13 hamlets (Klidon, Banjarsari, Wonosalam, Dongkelsari, Puntuk, Tanjung Sari, Karang Lo, Purworejo, Tanjung, Banturejo, Nglengkong and Surirejo), Sukoharjo Village, District Ngaglik, DIY Sleman regency. Data are collect and analysed in order to obtain results in a level of comfort. The analysis was performed by using a formula based on the comfort level Temperature Humidity Index (THI). The results showed that Hamlet Klidon, Banjarsari, Wonosalam, and Dongkelsari shows Not Comfortable, whereas Hamlet Puntuk, Tanjung Sari, Karang Lo, Purworejo, Tanjung, Banturejo, Nglengkong, Surirejo and Mujen show Strongly Comfortable mainly on the clock 10:00 to 15:00.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2016-04-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/34
10.14421/biomedich.2016.51.15-18
Biology, Medicine, & Natural Product Chemistry; Vol 5, No 1 (2016); 15-18
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/34/25
Copyright (c) 2016 Supriatna, Thaqibul Fikri Niyartama, Iwan Kuswidi
oai:ojs.sciencebiology.org:article/315
2023-08-09T16:42:43Z
BIOMEDICH:ART
Phytochemical Analysis and Antibacterial Activity of Methanol and Ethyl Acetate Extracts of Detarium microcarpum Guill. & Perr.
Dahiru, Mubarak Muhammad
Abaka, AbudulAzeez Mumsiri
Artimas, Susan Pwakangdi
Medicine Journal; Medicine; Natural Product; Natural Product Chemistry
Antibacterial activity; Antimicrobial activity; Antibacterial resistance; Detarium microcarpum; Phytochemical analysis
This research aimed to investigate the phytoconstituents and antibacterial effects of methanol and ethyl acetate stem bark extracts of Detarium microcarpum (DM). The phytochemicals were detected and quantified while the antibacterial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhi was established determining the zone of inhibition (ZI), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Phytochemical screening showed alkaloids (16.33% 0.88) were present in the methanol extract only while saponins and flavonoids were detected in concentrations of 31.00% 2.31 and 21.01% 2.33 respectively for the methanol extract and 21.67% 1.76 and 38.01% 1.16, for the ethyl acetate. The methanol extract exhibited the highest ZI on S. aureus (21.3 mm 1.11) with its least inhibition observed on E. coli (6.5 mm 0.77) while the ethyl acetate extract demonstrated the highest ZI on S. typhi (19.1 mm 2.01). S. typhi exhibited more sensitivity to DM extracts at the least concentrations of 12.5 mg/ml (methanol) and 25 mg/ml (ethyl acetate) while the MBC results showed that the 12.5 mg/ml and 25 mg/ml were the effective respective concentration for methanol and ethyl acetate extracts against S. typhi. Conclusively, DM exhibited an antibacterial effect against the test organisms with notable inhibitory and bactericidal effects.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-03-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/315
10.14421/biomedich.2023.121.281-288
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 281-288
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/315/193
Copyright (c) 2023 Mubarak Muhammad Dahiru, AbudulAzeez Mumsiri Abaka, Susan Pwakangdi Artimas
oai:ojs.sciencebiology.org:article/99
2020-04-06T11:50:09Z
BIOMEDICH:ART
A Simple Method for Clearing and Staining Specimens for The Demonstration of Animal Skeleton
Luthfi, Muhammad Ja’far
Soesilo, Nyoman Puniawati
skeleton; Alizarin Red S; Alcian Blue; elementary school; teaching aid
Teaching skeletal system would be more attractive using real/preserved specimen compared to those using only book. The aim of the research was to develop clearing and staining method of animal skeleton specimen using Alizarin Red S-Alcian Blue as teaching aid tool. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. The spesimen will increase effectiveness in elementary school science teaching and learning.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2019-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/99
10.14421/biomedich.2019.81.17-21
Biology, Medicine, & Natural Product Chemistry; Vol 8, No 1 (2019); 17-21
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/99/83
Copyright (c) 2019 Muhammad Ja’far Luthfi, Nyoman Puniawati Soesilo
oai:ojs.sciencebiology.org:article/398
2024-03-20T02:04:38Z
BIOMEDICH:ART
The Correlation Between Widal Diagnostic Test, Total Leukocyte Count, and Platelet Count in Suspected Typhoid Fever Patients at RS Aura Syifa Kediri
Loviana, Novi
Savitri, Lisa
Krissanjaya, Rochmad
Kasimo, Elfred Rinaldo
Medical Technology Laboratory; Biology; Immunology
Widal; leukocytes; platelets; S. Typhi
Typhoid fever is an acute systemic infectious disease that is related to poor personal hygiene and environmental sanitation. The diagnosis of typhoid fever is established through laboratory examinations, including serological, hematological, and bacteriological tests. This research aims to determine the correlation between the Widal diagnostic test and the total leukocyte count and platelet count in suspected typhoid fever patients at RS Aura Syifa Kediri. The study design used in this research is quantitative descriptive analysis with a cross-sectional approach. The data was collected retrospectively from secondary sources, specifically medical records of patients from August 1, 2021, to March 31, 2022, at RS Aura Syifa Kediri. The total population comprised 157 patients, and a purposive sampling method was used to select 41 patients who met the inclusion and exclusion criteria. The statistical analysis using the Spearman correlation test resulted in p-values ≥ α = 0.05 for the following correlations: Widal test O with leukocytes (0.538 ≥ 0.05), Widal test H with leukocytes (0.915 ≥ 0.05), Widal test O with platelets (0.476 ≥ 0.05), and Widal test H with platelets (0.965 ≥ 0.05). These findings indicate that there is no significant correlation between the Widal test O and H with the platelet count in patients with typhoid fever. Therefore, it can be concluded that there is no significant relationship between the Widal test O and H results and the platelet count in typhoid fever patients.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-09-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/398
10.14421/biomedich.2023.122.555-558
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 555-558
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/398/230
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/398/55
Copyright (c) 2023 Novi Loviana, Lisa Savitri, Rochmad Krissanjaya, Elfred Rinaldo Kasimo
oai:ojs.sciencebiology.org:article/148
2022-07-31T08:19:35Z
BIOMEDICH:ART
Effect of Diabetes Mellitus and Hypertension on Osmotic Fragility and Hemorheological Factors in Male Wistar Rats
Okonofua, David Ehikhuemen
Asiwe, Jerome Ndudi
Anachuna, Kenneth Kelechi
Moke, Emuesiri Goodies
Sanusi, Kamaldeen Olalekan
Adagbada, Ebunoluwa Oluwabusola
Yusuf, Mariam Onono
Alawode, Damilola Ifeoluwa
Fasanmade, Adesoji Adedipe
Medicine; Pharmacology; Physiology
diabetes mellitus; hemorheology; high salt diet; hypertension; viscosity
Diabetes mellitus is a common risk factor for erythrocyte osmotic stress. This study was aimed at exploring the effect of streptozotocin (STZ)-induced diabetes mellitus and salt-induced hypertension on osmotic fragility and hemorheological variables in male Wistar rats. Thirty male rats were grouped into five groups of six animals each as follows: negative control (zero salt in diet); positive control (normal salt diet - 0.3% salt); high salt diet (8% salt) (HSD only); STZ induced diabetes and normal salt diet (STZ only); STZ induced diabetes and high salt diet (STZ + HSD). At the end of a 4 weeks period, hematological variables, osmotic fragility, rheology and cardiovascular responses were assessed. There was an increase (p<0.05) in the mean arterial pressure and heart rate of HSD, STZ and HSD + STZ groups indicating a salt induced hypertension. There was a decrease in the body weight of STZ and HSD +STZ groups. There was significant increase (p<0.05) in the haematocrit, platelets estimates and fibrinogen concentrations in the experimental groups when compared with the controls. The STZ and STZ + HSD groups showed a reduced clotting time which corresponded to the increased platelet estimates and fibrinogen concentration. The increase in haematocrit, platelet and plasma protein resulted in the increased blood viscosity and a decreased flow rate. The osmotic fragility test was also observed to be increased (p<0.05) in HSD, STZ only and STZ + HSD groups. Diabetes mellitus and hypertension increase the rate of hemolysis of erythrocyte, as well as increase blood viscosity.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/148
10.14421/biomedich.2021.102.73-79
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 2 (2021); 73-79
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/148/122
Copyright (c) 2021 David Ehikhuemen Okonofua, Jerome Ndudi Asiwe, Kenneth Kelechi Anachuna, Emuesiri Goodies Moke, Kamaldeen Olalekan Sanusi, Ebunoluwa Oluwabusola Adagbada, Mariam Onono Yusuf, Damilola Ifeoluwa Alawode, Adesoji Adedipe Fasanmade
oai:ojs.sciencebiology.org:article/249
2023-08-09T16:42:43Z
BIOMEDICH:ART
Proximate Composition, Levels of Some Essential Mineral Elements and Anti-Nutritional Components of Some Yam Species Found in Minna, Niger State
Godfrey, Eneogwe Okechukwu
Esther, Ibrahim Izihyi
Faith, Obuye
Chemistry; Journal Natural product
proximate composition; anti-nutritional; mineral content; Dioscorea dumenturom; Dioscorea rotundata; Dioscorea cayenensis
Samples of Dioscorea dumenturom, Dioscorea rotundata and Dioscorea cayenensis were investigated for their proximate composition, anti-nutritional and mineral contents using standard analytical methods. These varieties of Dioscorea analysed showed a significant difference (P≤0.05) amongst them. However, from the results, it was observed that Dioscorea rotundata had the highest ash (8.05±0.05 %) and crude fibre content (13.11±0.10 %) which indicates that it contains more mineral stuffing and is best for softening of stool. Dioscorea cayenensis had the highest fat content (16.31±0.30 %), indicating that it is a better source of calories than other yam species analysed. Dioscorea dumenturom had the lowest moisture content (3.51±0.01 %) as well as the highest crude protein (12.29±0.01 %) and carbohydrates (69.04±0.10 %) than other yam species analysed, indicating its longer shelf-life, high bodybuilding capacity and better source of energy than other yam species analysed. The anti-nutritional constituent of alkaloid and tannin were lowest in Dioscorea cayenensis while Dioscorea rotundata had the least cyanide, phytate and oxalate content. This implies that these particular yams are safer for consumption. The elemental analysis in mg/100g indicated that the yam species contained appreciable levels of essential minerals, with Dioscorea dumenturom having the highest sodium, calcium, iron, potassium, phosphorous and magnesium concentration of 32.05±0.07 mg/100g,190.57±0.01mg/100g, 5.98±0.03 mg/100g, 80.12±0.17 mg/100g, 237.10±0.48 mg/100g and 100.22±0.03 mg/100g respectively. All these mineral concentrations exist within the permissible limit of WHO and hence indicate that the yam species can serve as a good source of minerals.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Godfrey Okechukwu Eneogwe, Federal University Lokoja
Esther Izhiyi I brahim, Federal University Lokoja
Faith Obuye, Federal University Lokoja
2022-09-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/249
10.14421/biomedich.2023.121.9-16
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 9-16
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/249/162
Copyright (c) 2022 Eneogwe Okechukwu Godfrey, Ibrahim Izihyi Esther, Obuye Faith
oai:ojs.sciencebiology.org:article/275
2023-08-09T16:42:43Z
BIOMEDICH:ART
Bioactive Compound in Solanum torvum and Its Potential as Functional Food and Drink: A Review
Putri, Nina Nurazizah Purnomo
Anggriani, Rista
Sukardi, Sukardi
food technology
Solanum torvum, antioxidant, bioactive compound, nutrition, functional food and drink.
Solanum torvum is a fruit used as food and has medical properties. This study aims to provide an updated understanding of Solanum Torvums health benefits as a functional food through a study literature review. The research utilizes electronic databases (PubMed, Science Direct, Scopus, Nature, Clinical Key, and Springer) from July-October 2022. Solanum Torvum contains various bioactive components, vitamins, mineral, nutritions. It shows that Solanum torvum has potential sources as functional food. However, studies about solanum torvum consumption as a functional food and the clinical trial of its health benefits in humans are still limited. Further in vivo and in vitro studies are necessary to present the effect of solanum torvum consumption on health.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
University of Muhammadiyah Malang
2023-02-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/275
10.14421/biomedich.2023.121.205-213
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 205-213
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/275/183
Copyright (c) 2023 Nina Nurazizah Purnomo Putri, Rista Anggriani, Sukardi
oai:ojs.sciencebiology.org:article/78
2020-04-11T03:13:43Z
BIOMEDICH:ART
Degradation Study of Biodegradable Plastic Using Nata De Coco as A Filler
Elfiana, Tiara Nur
Fitria, Anisa Nur Izza
Sedyadi, Endaruji
Prabawati, Susy Yunita
Nugraha, Irwan
Biodegradable; Cellulose; Ganyong canna; Nata de coco
Starch is known as a biodegradable raw material that can be degraded by bacteria and microorganisms in the soil. Starch has cellulose which is kind of plant cellulose. This study shows the biodegradation rates of plastic made from Ganyong Canna (Canna edulis Kerr) as a cellulose source which is added with nata de coco as a filler. The biodegradable plastic functional group was confirmed by using FITR. The results show that the O-H group of Ganyong Canna (Canna edulis Kerr) biodegradable plastic is located at wave number 3298.03 cm-1 and shifted to 3290.32 cm-1 after addition of nata de coco. The C-H bonds functional groups in Canna biodegradable plastics and nata de coco plastics are at wave numbers 2920.01 cm-1 and 2916.16 cm-1. While the C-O bonds functional groups in biodegradable starch plastics and nata de coco is shown at wave numbers 995.05 cm-1. The mechanical properties of biodegradable plastics testing are thickness, tensile strength, and elongation based on the ASTM method. The thickness is about 0.1005 mm, the tensile strength of biodegradable plastic is 4,3244 MPa and the elongation value range about 13.9639% while the WVTR range about 14.20 g/m² hours. The results show that the increase of the plastic degradation made from nata de coco occurs between 5% - 38% per days. It is faster than the plastic made from pure Ganyong Canna (Canna edulis Kerr) starch. These results indicate that nata de coco could be added in biodegradable plastic on packaging materials for better degradation.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2018-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/78
10.14421/biomedich.2018.72.33-38
Biology, Medicine, & Natural Product Chemistry; Vol 7, No 2 (2018); 33-38
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/78/67
Copyright (c) 2018 Tiara Nur Elfiana, Anisa Nur Izza Fitria, Endaruji Sedyadi, Susy Yunita Prabawati, Irwan Nugraha
oai:ojs.sciencebiology.org:article/375
2024-03-20T02:04:38Z
BIOMEDICH:ART
Nephroprotective Activities of Ethanol Root Extract and Fractions of Hippocratea africana Against Doxorubicin-Induced Kidney Toxicity
Noah, Kufre U.
Udobang, John A.
Okokon, Jude E.
Anagboso, Martin O.
Ebong, Nwakaego Omonigho
Renoprotective; Hippocratea africana; doxorubicin; oxidative stress
Hippocratea africana root used locally in the treatment of poisoning was investigated to confirm its antidotal potential in rats. The root extract (200-600 mg/kg) and fractions; dichloromethane (DCM) and aqueous, 400 mg/kg) were evaluated for nephroprotective activity against doxorubicin-induced kidney injury in rats. Kidney function parameters, kidney oxidative stress markers and kidney histology were used to assess the kidney protective effect of the extract. The root extract and fractions (200-600 mg/kg) significantly (p<0.05-0.01) reduced the levels of creatinine, urea and electrolytes that were elevated by doxorubicin. Also, the MDA level elevated by doxorubicin was reduced by the extract and fractions co-administration, while the levels of GSH, GST, SOD, GPx, and CAT that were decreased by doxorubicin were significantly (p<0.01) elevated by the root extract/fractions. Histology of the kidney sections of extract/fractions -treated animals showed reductions in the pathological features compared to the organotoxic-treated animals. The chemical pathological changes were consistent with histopathological observations suggesting marked nephroprotective potential. The anti-toxic effect of this plant may in part be mediated through the chemical constituents of the plant. The plant, Hippocratea africana possesses anti-toxicant properties which can be exploited in the treatment of doxorubicin related toxicities.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-08-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/375
10.14421/biomedich.2023.122.477-484
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 477-484
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/375/216
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/375/50
Copyright (c) 2023 Kufre U. Noah, John A. Udobang, Jude E. Okokon, Martin O. Anagboso, Nwakaego Omonigho Ebong
oai:ojs.sciencebiology.org:article/135
2022-01-11T09:12:17Z
BIOMEDICH:ART
Production and Optimization of Pectinase from Pectinolytic Fungi Cultivated on Mango peels and Pectin Subjected to Submerged Fermentation
Mulluye, Kelemu
Kebede, Ameha
Bussa, Negussie
Mango peels; Pectinase; Pectin; Submerged fermentation
Pectinases are the group of enzymes that degrade pectin. This study was conducted with the aim of isolation of efficient pectinase producing pectinolytic fungi from the decomposing mango peels using extracted mango peels pectin as a growth substrate under submerged fermentation, determining optimum pectinase production conditions with regards to some physicochemical parameters. The organisms were screened for the production of pectinase using Pectin agar media, and the two active pectinolytic fungi (P1 and P2) were isolated. pectinase production media was later used for the Lab scale production of pectinase by inoculating p1 and p2 and incubating for 7 days. The enzyme was extracted after seven days of fermentation and every day tested for their pectinolytic activity. P2 showed relatively higher pectinolytic activity and was therefore used for further studies. P2 was inoculated into a broth containing mango pectin under submerged fermentation. Results indicate that a pectin yield of mango peel 17.75%. Different parameters optimization processes were investigated on submerged fermentation namely pH, incubation period, temperature and substrate concentration optima were found 6, 4 days, 35oC and 1.5% respectively. The result suggests that mango peels have high pectin content and can be used for the value-added synthesis of pectinase.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/135
10.14421/biomedich.2021.101.15-21
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 1 (2021); 15-21
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/135/108
Copyright (c) 2021 Kelemu Mulluye, Ameha Kebede, Negussie Bussa
oai:ojs.sciencebiology.org:article/206
2022-09-20T02:36:20Z
BIOMEDICH:ART
The Triterpenes of Kageneckia oblonga
Tapia-Merino, Jorge
Arrue, Lily
Martín, José San
Muñoz, Orlando
Natural products
Kageneckia oblonga; motiol; triterpene derivative; hopane
Three known triterpenes were isolated from leaf extracts of Kageneckia oblonga by conventional chromatographic methods: ursolic acid, benthamic acid and a third called Kc-III. The structure of Kc-III was determined by RMN spectroscopy, FT-IT and HR-MS. The compound was identified as fern-7-en-3β-ol (motiol), not previously reported in Kageneckia.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-08-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/206
10.14421/biomedich.2022.112.133-136
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 2 (2022); 133-136
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/206/148
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/206/33
Copyright (c) 2022 Jorge Tapia-Merino, Lily Arrue, José San Martín, Orlando Muñoz
oai:ojs.sciencebiology.org:article/25
2018-08-20T01:52:43Z
BIOMEDICH:ART
The Effect Of Intensive Keramba On The Presence Of Parasite Organisms In Rivers Of Lingsar Area
Supriadi, Supriadi
Janah, Maratun
Intensive keramba; parasites organism; diversity index
The application of intensive keramba in rivers could affect the presence of parasite organisms throughout the river downstream. The aims of this research are to find out the diversity of parasite species and the effect of intensive aquaculture method developed by the community on the presence of various parasitic organisms, particularly in the downstream area. A total of 65 Tilapia fish samples (O. niloticus) that was collected from 3 areas ( 15 samples from upstream, 25 samples in keramba and 25 samples from downstream areas) have been examined in the laboratory of Faculty of Mathematic and Natural Science, University of Mataram. Methods employed to identify parasites that infected fish samples are native method and flotation method. This research has identified 7 species of parasites which were divided into 2 groups: ectoparasites (Trichodina sp., Amylodinium sp., Oogonium sp., Dactylogirus sp., Trematode) and endoparasites (Entamoeba sp. dan Camallanus sp.). Diversity index calculation indicated that parasite organisms in upstream area were lower in number than that in the downstream and intensive karamba area (H’= (0,825; 1,596 dan 1.324 respectively). These data has showed there was a difference in species diversity and evenness index of parasite organisms in the upstream, downstream and intensive keramba area. In conclusion, there was significant influence of the application of intensive keramba on the appearance of various parasite organisms that could affect the sustainability of fish aquaculture.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/25
10.14421/biomedich.2015.41.11-15
Biology, Medicine, & Natural Product Chemistry; Vol 4, No 1 (2015); 11-15
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/25/13
Copyright (c) 2015 Supriadi, Maratun Janah
oai:ojs.sciencebiology.org:article/285
2023-08-09T16:42:43Z
BIOMEDICH:ART
Ethnobotanical Survey of Aromatic and Medicinal Plants Used in Traditional Medicine and Agri-Food in The Fez-Meknes Region
El Finou, Hamza
Salhi, Nadia
Halmoune, Asma
El Rhaffari, Lhoussaine
Biology; Medicine; Natural Product; Natural Product Chemistry; Journal Natural Product;
Agri-food; ethnobotany; medicinal plants; monograph; traditional medicine
In order to identify the medicinal plants used in agri-food and traditional medicine by the population of Fez-Meknes region (Morocco), a floristic and ethnobotanical study was carried out in 4 provinces of this region (Fez, Meknes, Azrou, Taza). Questionnaire forms were used to survey the usual users of the plants and herbalists and to collect as much information as possible on the therapeutic and dietary use of aromatic and medicinal plants in the region. For plants used for food, our survey identified 29 species divided into 26 genera and 16 families, including herbaceous plants (70%), trees (16.60%), shrubs (10%) and bushes (3.30%). The study of the medicinal flora used in traditional medicine also allowed the inventory 81 species belonging to 47 families. Leaves are the most commonly used part and the majority of remedies were prepared in the form of infusion (47%) and decoction (26%). Among all the diseases treated, digestive diseases are the most cited (25%), followed by dermatological diseases (21%). The present study allowed us to evaluate some traditional practices used by the Fez-Meknes region population. In this context, it is essential to carry out similar investigations in other regions of the kingdom, in order to safeguard this precious natural heritage by means of a monograph that is as complete as possible and to validate the remedies and preparations identified using rigorous scientific protocols.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-01-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/285
10.14421/biomedich.2023.121.133-141
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 133-141
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/285/174
Copyright (c) 2023 Hamza El Finou, Nadia Salhi, Asma Halmoune, Lhoussaine El Rhaffari
oai:ojs.sciencebiology.org:article/49
2018-08-20T01:50:51Z
BIOMEDICH:ART
Clustering of 18 Local Black Rice Base on Total Anthocyanin
Kristamtini, Kristamtini
Wiranti, Endang Wisnu
Clustering; black rice; exploration; total anthocyanin
Black rice has a high anthocyanin content in the pericarp layer, which provides a dark purple color. Anthocyanin serve as an antioxidant that control cholesterol level in the blood, prevent anemia, potentially improve the body's resistance to disease, improve damage to liver cells (hepatitis and chirrosis), prevent impaired kidney function, prevent cancer/tumors, slows down antiaging, and prevent atherosclerosis and cardiovascular disease. Exploration results at AIAT Yogyakarta, Indonesia from 2011 to 2014 obtained 18 cultivar of local black rice Indonesia. The names of the rice are related to the color (black, red or purple) formed by anthocyanin deposits in the pericarp layer, seed coat or aleuron. The objective of the study was to classify several types of local black rice from explorations based on the total anthocyanin content. The study was conducted by clustering analyzing the total anthocyanin content of 18 local black rice cultivars in Indonesia. Cluster analysis of total anthocyanin content were done using SAS ver. 9.2. Clustering dendogram shows that there were 4 groups of black rice cultivars based on the total anthocyanin content. Group I consists of Melik black rice, Patalan black rice, Yunianto black rice, Muharjo black rice, Ngatijo black rice, short life of Tugiyo black rice, Andel hitam 1, Jlitheng, and Sragen black rice. Group II consists of Pari ireng, Magelang black hairy rice, Banjarnegara-Wonosobo black rice, and Banjarnegara black rice. Group III consists of NTT black rice, Magelang non hairy black rice, Sembada hitam, and longevity Tugiyo black rice. Group IV consist only one type of black rice namely Cempo ireng. The grouping result indicate the existence of duplicate names among the black rice namely Patalan with Yunianto black rice, and short life Tugiyo with Andel hitam 1 black rice.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2017-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/49
10.14421/biomedich.2017.62.47-51
Biology, Medicine, & Natural Product Chemistry; Vol 6, No 2 (2017); 47-51
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/49/40
Copyright (c) 2017 Kristamtini Kristamtini, Endang Wisnu Wiranti
oai:ojs.sciencebiology.org:article/297
2023-08-12T13:43:39Z
BIOMEDICH:ART
Peptide Fractions from Pepsin-digested Moringa oleifera Seed Proteins Inhibit Hemoglobin Glycation and Carbohydrate-hydrolyzing Enzymes
Ekun, Oluwafemi Emmanuel
Moringa oleifera; Peptide; Pepsin; Hemoglobin; ?-Amylase; ?-Glucosidase
The multidirectional abilities of peptide digests and fractions obtained from the hydrolysis of food-based proteins have been investigated in recent times. This study aims to evaluate the effects of pepsin-derived Moringa oleifera seed protein hydrolysates and fractions on hemoglobin glycation and the carbohydrases - ?-amylase and ?-glucosidase. Proteins were extracted from M. oleifera seeds and consequently digested using pepsin. The hydrolysates obtained were separated into fractions of <1 kD, 1-3 kD, and 3-5 kD ranges using size-exclusion chromatography and comparison with elution volumes of known standards. The activities of the hydrolysates and peptide fractions against both the non-enzymatic glycation of hemoglobin and the carbohydrases were determined in vitro. Results revealed that the hydrolysate and its peptide fractions demonstrated varying abilities against the glycation of hemoglobin, with the unfractionated hydrolysate showing better activities (78.230 0.774 % at a maximum concentration of 1.0 mg/ml) than its peptide fractions. Also, the hydrolysates and fractions demonstrated higher inhibitory effects on a-amylase (with all fractions displaying above 50% inhibition at a final concentration of 1.0 mg/mL) than against a-glucosidase. Kinetic analysis of a selected fraction showed that it inhibited ?-amylase via a mixed mechanism (Ki = 0.029 mg/mL) but displayed an uncompetitive mode for ?-glucosidase inhibition (Ki = 0.333 mg/mL). Therefore, it is inferred that M. oleifera seed proteins encode potentially therapeutic peptide sequences that could be further processed to formulate potential antidiabetic agents.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/297
10.14421/biomedich.2023.121.413-422
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 413-422
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/297/206
Copyright (c) 2023 Oluwafemi Emmanuel Ekun
oai:ojs.sciencebiology.org:article/119
2021-07-22T06:52:01Z
BIOMEDICH:ART
The Role of Gene Therapy in the Treatments of Type 1 Diabetes Mellitus: a review
Smail, Harem Othman
Biology
Type 1 diabetes mellitus; Gene therapy; β cells; Virus vectors and islet
The main aims of this review were to understand the roles of gene therapy in the treatment and prevention of type 1 diabetes mellitus and I will discuss a brief history, approaches, vector types with the future of diabetes following clinical use. Type 1 diabetes mellitus is a metabolic condition that is identified by insufficient insulin development due to pancreatic damage to beta cells. Control, long life, and diagnosis of these metabolic disorders have become vital sources for many scientists and researchers. After 2000, the latest approaches to molecular medicine were introduced as one of the possible therapeutic options for diabetes type 1 diagnosis. Many genes have been reported as a clinical trial so that damaged genes can be treated and three main approaches shown about 50 years ago are islet transplantation, β cell regeneration, and insulin gene therapy to cure and prevent diabetes type. Treating diabetes through gene therapy can promise children and adolescents, but more clinical applications are needed to recognize it as a permanent route.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2020-11-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/119
10.14421/biomedich.2020.92.57-64
Biology, Medicine, & Natural Product Chemistry; Vol 9, No 2 (2020); 57-64
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/119/99
Copyright (c) 2020 Harem Othman Smail
oai:ojs.sciencebiology.org:article/440
2024-03-20T02:04:38Z
BIOMEDICH:ART
Identification and Toxicity Profiling of Column Fractions of Ethanol Leaf Extract of Ziziphus mauritiana
Owolarafe, Tajudeen Alowonle
Kailani, Salawu
Biochemistry; Natural Product; bioactivities
Dioscoreside C; chromatographic fractions; Ziziphus mauritiana; toxicity
Different plants may contain varying amounts of phytochemicals and also different degrees of toxicity. This study aimed to evaluate the phytochemicals present in the most active column fraction of ethanol leaf extract of the Ziziphus mauritiana plant and their toxic effects using brine shrimp lethality assay and animal model. Crude ethanol extract was obtained by maceration while fraction was achieved using a column chromatography experiment. Toxicity was evaluated with brine shrimp lethality assay and albino rat’s models while characterization was achieved with liquid chromatography-mass spectrophotometry. Ziziphus mauritiana leaves revealed 42 fractions pooled into seven fractions. Fraction three (3) was the most toxic with the brine shrimp lethality assay of (31.48ug/ml) and its toxicological evaluation revealed an adverse effect on the hematological parameter, biochemical indices, and histo-architecture of the liver and kidney of the experimental model studied. LCMS analysis of the most toxic fraction revealed the presence of Antirrhinoside, Lucidumol A, Apigenin 7-glucuronide-4'-(6''-malonylglucoside), Dioscoreside C, Camellioside D, and others which have been reported for various pharmacological effects including adverse effects, The mode of toxicity may be synergistic, individual, or antagonistic which may explain the moderate toxicity observed in animal model. Administration of these fractions may lead to toxicity despite their potential.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
TETFUND (Tertiary Trust Fund)
2024-02-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/440
10.14421/biomedich.2023.122.651-662
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 651-662
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/440/242
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/440/58
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/440/59
Copyright (c) 2024 Tajudeen Alowonle Owolarafe, Salawu Kailani
oai:ojs.sciencebiology.org:article/168
2022-07-31T01:51:06Z
BIOMEDICH:ART
Liver and Renal Cell Damage Following Excess Bee Honey Consumption in Male Wistar Rat
Agbatutu, Akpevwoghene
Asiwe, Jerome Ndudi
Adebayo, Olusegun Gafar
Biology; Natural product;
Honey; Excess consumption; liver enzyme; electrolyte; renal function; haematological indices; histopathology
Honey is a widely used natural product with several health benefits. However, there is paucity of information on its excessive usage. The present study investigated the effect of excess honey consumption on hepato-renal functions in male wistar rats. Twenty-eight adult male Wistar rats were selected into four groups (n=7) and treated with distilled water (control) and 1ml, 2ml and 3ml of honey respectively for 5 weeks. Thereafter, the animals were euthanised and blood as well as kidney and liver were collected for further studies. There was a significant increase in Creatinine, Bilirubin, Urea AST, ALP, total protein as well as a significant decrease in RBC, WBC, haemoglobin, lymphocyte and PCV. Histology of the liver and kidney revealed a significant degeneration and necrosis in a dose dependent manner. This study suggest that excess honey consumption causes liver and renal cellular damage as well as haematological alterations.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/168
10.14421/biomedich.2022.111.35-43
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 1 (2022); 35-43
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/168/138
Copyright (c) 2022 Akpevwoghene Agbatutu, Jerome Ndudi Asiwe, Olusegun Gafar Adebayo
oai:ojs.sciencebiology.org:article/13
2018-08-20T01:53:05Z
BIOMEDICH:ART
The Effect of Water-Soluble Stem Extract “Kayu Kuning“ (Arcangelisia flava L.Merr) On The Growth Inhibition of Candida albicans ATCC 10231 and Trichophyton mentagrophytes IN VITRO
Setyowati, Rini
Sudarsono, Sudarsono
P, Setyowati E
Arcangelisia flava L.Merr; “kayu kuning”; growth inhibitor; Candida albicans; Trichophyton mentagrophytes
“Kayu kuning” (Arcangelisia flava L.Merr) was used when someone has a skin problem caused by Candida albicans and Trichophyton mentagrophytes. Scientific based medicine on this traditional knowledge was necessary be done. Stem powderwas extracted by distilled water.The extract was then evaporated. Qualitative and quantitative analysis of the active substance e.g., Berberin chloride by Thin Layer Chromatography (TLC) The antifungal activity againts Candida albicans and Trichophyton mentagrophyteswere tested by using agar diffusion and microdilution methods. The absorbance from microdilution were analized by One way ANOVA. The conclusion showed that the extract contained 1.55±0.12% w/walkaloid calculated as Berberine chloride. The inhibition zone for Candida albicans and Trichophyton mentagrophytes were 16.65±4.52 and 6.55±0.05 mm respectively. The MIC vallue for both fungi was 10 mg/mL.The MBC value for Candida albicans was 40 mg/mL and for Trichophyton mentagrophytes was 50 mg/mL. From the analysis with one-way ANOVA, shows that there are significant differences between the positive control group and the test solution with the negative control group with p=0.020 for Candida albicans and p=0.028 for Trichophyton mentagrophytes (p<0.050). Post hoc Tukey analysis results showed that both inter-group and between the concentration of the test solution to the control group did not differ significantly positive because the value of p>0.050.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2014-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/13
10.14421/biomedich.2014.31.15-19
Biology, Medicine, & Natural Product Chemistry; Vol 3, No 1 (2014); 15-19
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/13/2
Copyright (c) 2014 Rini Setyowati, Sudarsono, Setyowati E P
oai:ojs.sciencebiology.org:article/41
2018-08-20T01:52:01Z
BIOMEDICH:ART
Histological Study of Common House Gecko (Hemidactylus frenatus) Regenerated Tail
Rakhmiyati, Rakhmiyati
Luthfi, Muhammad Jafar
Common house gecko (Hemidactylus frenatus); autotomy; tail histology; cartilaginous tube of regenerated tail
Common house gecko (Hemidactylus frenatus) belongs to suborder Lacertilia that has capacity to shed its tail (autotomy) as a self-defense mechanism. After autotomy, tail regeneration occurs. Axiale skeleton of an original tail is composed of bony vertebrae, whereas the the regenerate one is comprised of cartilaginous tube. The purpose of this study was to determine the histological difference between axial skeleton of the original tail and the regenerate one of the common house gecko. Twenty four individuals consist of twelve common house gecko with original tail and twelve with regenerate tail were used. Microanatomical observations were carried on histological slide of original and regenerated tail stained with Hematoxylin-Eosin and Mallory Acid Fuchsin. The results showed that the original tail comprised of bony vertebrae whereas regenerated tail supported by cartilaginous tube.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2016-10-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/41
10.14421/biomedich.2016.52.49-53
Biology, Medicine, & Natural Product Chemistry; Vol 5, No 2 (2016); 49-53
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/41/31
Copyright (c) 2016 Rakhmiyati Rakhmiyati, Muhammad Jafar Luthfi
oai:ojs.sciencebiology.org:article/314
2023-08-12T13:44:13Z
BIOMEDICH:ART
Uncovering the Antioxidant Power: Investigating the Skin and Flesh of Crystal Guava with Chloroform and Methanol Extractions and DPPH Assay
Ulmillah, Aulia
Alghifari, Arif
Widiani, Nurhaida
Natural Product; Natural Product Chemistry; Journal Natural Product;
Antioxidant; DPPH; Chloroform; Crystal guava; Fruit flesh; Methanol; Peel;
Crystal guava (Psidium guajava var. crystal) is one type of guava plant that has high economic value due to its thick flesh and few seeds. The crystal guava plant is believed to have antioxidant activity, which is a substance that can prevent the formation of free radicals in the body. This research aims to determine the level of antioxidants in chloroform and methanol extracts of the skin and flesh of crystal guava fruit using the DPPH method. Samples were taken through a stepwise maceration process and solvents of chloroform and methanol, then analyzed using probit analysis and SPSS 25 software. The results of the study showed that both chloroform and methanol extracts of the skin and flesh of crystal guava fruit have antioxidant activity. The results showed that the IC50 value of the chloroform extract of crystal guava fruit skin is 218.88 ppm and is classified as moderate, the methanol extract of crystal guava fruit skin is 89.78 ppm and is classified as strong, the chloroform extract of crystal guava fruit flesh is 270.56 ppm and is classified as weak, and the methanol extract of crystal guava fruit flesh is 185.72 ppm and is classified as moderate.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-03-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/314
10.14421/biomedich.2023.121.323-328
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 323-328
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/314/195
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/314/42
Copyright (c) 2023 Aulia Ulmillah, Arif Alghifari, Nurhaida Widiani
oai:ojs.sciencebiology.org:article/92
2020-04-11T02:52:54Z
BIOMEDICH:ART
Liver Profile of Atazanavir/Ritonavir in Pregnant Albino Rats
Adikwu, Elias
Kemelayefa, James
Ocheiga, Winifred
Medicine;
Atazanavir/ritonavir; pregnancy; liver; toxicity; rats
Medication use during pregnancy is challenging due to the occurrence of maternal or fetal toxicities. Atazanavir/ritonavir (ATV/r) has hepatotoxic potential hence; use in pregnant patients living with human immunodeficiency virus may cause maternal hepatotoxicity. This study assessed the liver profile of ATV/r in pregnant albino rats. Thirty pregnant albino rats randomized into groups were orally treated daily with ATV/r (4.28/1.43 mg/kg-34.3/11.4 mg/kg) for 16 days. After treatment, the rats were weighed and sacrificed. Blood samples were collected and examined for serum biochemical parameters. Liver samples were weighed and assessed for biochemical and histological changes. Body and liver weights were normal (p>0.05) in ATV/r-treated pregnant rats when compared to control. Serum total cholesterol, triglyceride, low density lipoprotein cholesterol and blood glucose levels were significantly (p<0.01) elevated whereas high density lipoprotein cholesterol level was significantly (p<0.01) decreased in rats treated with ATV/r (34.3/11.4 mg/kg) when compared to control. Liver and serum aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase, total bilirubin, and conjugated bilirubin levels were significantly increased in a dose-dependent fashion in rats treated with ATV/r; 8.57/2.86 mg/kg (p<0.05), 17.1/5.72 mg/kg (p<0.01) and 34.3/11.4 mg/kg (p<0.001) when compared to control. Liver superoxide dismutase, catalase, glutathione and glutathione peroxidase levels were significantly decreased whereas malondialdehyde levels were significantly increased in a dose-dependent fashion in rats treated with ATV/r; 8.57/2.86 mg/kg (p<0.05), 17.1/5.72 mg/kg (p<0.01) and 34.3/11.4 mg/kg (p<0.001) when compared to control. Necrotic hepatocytes were observed at higher doses of ATV/r. ATV/r may not be hepatotoxic in pregnant women living with HIV at the clinical dose.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/92
10.14421/biomedich.2019.82.47-52
Biology, Medicine, & Natural Product Chemistry; Vol 8, No 2 (2019); 47-52
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/92/89
Copyright (c) 2019 Elias Adikwu, James Kemelayefa, Winifred Ocheiga
oai:ojs.sciencebiology.org:article/386
2024-03-20T02:04:38Z
BIOMEDICH:ART
Quercetin Bioavailability Evaluation on Standardized Herbal Medicine Containing Guava Leaf Extract with HPLC
Yulion, Rizky
Yulianis, Yulianis
Suntri, Suntri
Natural Product; Natural Product Chemistry; Journal Natural Product;Biological
HPLC; Quercetin; Bioavailability; Standardized Herbal Medicine
Standardized herbal medicines are classified as one of Indonesia's natural medicine ingredients in addition to herbal medicine and phytopharmaceuticals. The safety and efficacy of standardized herbal medicines are scientifically proven through preclinical trials, and raw materials and products have been standardized. One includes standardized herbal medicines is diapet, psidii, lelap, fitolac, and glucogarp. To determine the bioavailability of standardized herbal products containing guava leaf extract (Psidium guajava L.). which uses single and combined natural materials. The method used is experimental with a crossover design. Blood samples are taken from the marginalis vein of the rabbit ear at 0.5 hours; 1; 2; 4; and 6. The level of quercetin in the blood is determined by the reverse-phase HPLC method. The mobile phase used is methanol:aquabidest (59:41,v/v), stationary phase octadecyl silica (C18), flow rate 1 mL/min, UV-Vis detector 370 nm, and injection volume 20 ?L. The value of bioavailability parameters obtained in the parameters Cpmax, Tmax, and AUC of product A is 1.486454 ?g / ml; 1.4 hours and 10.2615291 ?g/ml/h, product B is 1.29224019 ?g/ml; 1.5 hours and 11.30810501 ?g/ml/hour. Based on the results of this study, it can be concluded that the bioavailability profile of the two products is not much different, so it is expected that the effects caused are the same.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-11-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/386
10.14421/biomedich.2023.122.593-599
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 593-599
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/386/221
Copyright (c) 2023 Rizky Yulion, Yulianis Yulianis, Suntri Suntri
oai:ojs.sciencebiology.org:article/162
2022-07-31T08:19:35Z
BIOMEDICH:ART
Acute Toxicity and Hypoglycemic Effect of a Polyherbal Formulation on Blood Glucose in Oral Glucose Tolerance Test (OGTT) and Alloxan-Induced Diabetic Rats
Warri, Abednego Okeoghene
Moke, Emuesiri Goodies
Balogun, Aishat Oyinkansola
Nzeh, Kennedy Chibogu
Umukoro, Emuesiri Kohworho
Erhirhie, Earnest Oghenesuvwe
alloxan monohydrate; diabetes mellitus; herbal formulation; LD50 value
Madam F. Kayes Bitters® is an herbal formulation commonly used in Nigeria and some African countries in the management of diabetes mellitus and other diseases conditions. This study evaluated the in-vivo hypoglycaemic activity, as well as acute toxicity of the polyherbal formulation to provide its efficacy and safety. Healthy albino mice (20-30 g) and Sprague Dawley female rats (90-130 g) were used for this study. Acute toxicity study (LD50) of the herbal formulation was determined by methods originally described by Miller and Tainter in 1994. Following oral dosing with glucose (2 g/kg) in normal fasted animals, herbal formulation (HF) at various doses was administered and blood glucose levels at 30 minutes, 60 minutes, 90 minutes, and 120 minutes were taken and recorded. Diabetes was induced using alloxan 150 mg/kg and diabetic rats were given the HF at doses of 50, 100, and 200 mg/kg with glibenclamide 2.5 mg/kg used as standard drug treatment. Blood glucose level was determined on 1st day, 7th day, 14th and 21st day. The LD50 was greater than 5g/kg with oral administration. The oral glucose tolerance test showed that the group that received 100 mg/kg HF showed a significant reduction (p<0.05) in glucose level after 120 minutes when compared to the basal level of glucose recorded. All treated diabetic groups showed a significant decrease in glucose level on the 21st day. The herbal formulation of Hydrastis canadesis Aloe capensis, Echinacea angustifolia and honey exhibited a significant glucose-lowering activity in alloxan-induced diabetic rats.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-10-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/162
10.14421/biomedich.2021.102.111-115
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 2 (2021); 111-115
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/162/128
Copyright (c) 2021 Abednego Okeoghene Warri, Emuesiri Goodies Moke, Aishat Oyinkansola Balogun, Kennedy Chibogu Nzeh, Emuesiri Kohworho Umukoro, Earnest Oghenesuvwe Erhirhie
oai:ojs.sciencebiology.org:article/269
2023-08-09T16:42:43Z
BIOMEDICH:ART
Acute Toxicity LD50 Fraction Ethyl Acetate Aquilaria malaccensis, Ficus benjamina, Mikania micrantha, and Fraction Water Cinnamomum burmanii in Mus Musculus
Yulion, Rizky
Perawati, Santi
Hartesi, Barmi
Anggresani, Lia
Andriani, Lili
Indriani, Lesra
Syahila, Lara
Ramadani, Suci
Monika, Nadia
Natural Product, Pharmacology, Ethnopharmacology
Beringin leaf (Ficus benjamina L.); Gaharu leaf (Aquilaria malaccensis); Kayu manis cortex (Cinnamomum burmanii); Sembung rambat leaf (Mikania micrantha Kunth); Toxicity acute LD50.
Research on the acute toxicity of LD50 had been carried out on the ethyl acetate fraction of Gaharu leaves (Aquilaria malaccensis), beringin leaves (Ficus benjamina), sembung rambat leaves (Mikania micrantha), and kayu manis cortex (Cinnamomum burmanii) water fraction the base of use as traditional treatment by the Suku Anak Dalam (SAD) empirically obtained from ancestors. This research methodwas experimental and it used male and female mice. The control group was given a 1% NaCMC doses of 625 mg/kg body weight, 1,250 mg/kg body weight, 2,500 mg/kg body weight, and 5,000 mg/kg body weight. The test preparation was administered orally once per day. The Thomson-Weil method was used for the measurement of LD50 values. The weight ratio of organs was applied for heart, liver, lungs, kidneys, and stomach. The results showed that the LD50 value for male mice was 2454mg/kg body weight; 2454mg/kg body weight; 1546 mg/kg body weight and 2065mg/kg body weight respectfully. The LD50 value for female mice was a pseudo LD50. The value of the organ weight ratio in each sample showed P<0.05 values that were obtained in gaharu leaves in the liver and kidney organs (male); heart in females. Beringin leaves value P<0.05 lung organs (male). Sembung rambat leaves P<0.05 values of the heart, liver, and stomach organs (male); heart and stomach (female). Cinnamomum cortex value P<0.05 liver and stomach organs (male). The conclusions showed that the LD50 value of the ethyl acetate fraction of gaharu leaf, sembung rambat leaf, beringin leaf, and kayu manis cortex in male mice was slightly toxic, while in female mice it could not be counted.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2022-10-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/269
10.14421/biomedich.2023.121.55-60
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 55-60
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/269/167
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/269/38
Copyright (c) 2022 Rizky Yulion, Santi Perawati, Barmi Hartesi, Lia Anggresani, Lili Andriani, Lesra Indriani, Lara Syahila, Suci Ramadani, Nadia Monika
oai:ojs.sciencebiology.org:article/30
2018-08-20T01:52:35Z
BIOMEDICH:ART
Effect of Lunasia amara Blanco on Sperm Number, Sperm Motility, and Testicular Histology of Male Rats
Luthfi, Muhammad Ja’far
Department of Biology
Sanrego; male fertility; testis histology; sperm quality; sperm number; sperm motility
Sanrego (Lunasia amara), has been used in the folk medicine to increase and/or to treat male fertility. However there is no scientific evidence to confirm the positive effect of the plant on an improvement of male fertility. The objective of this research was to study the effects of the plant (on adult Sprague-Dawley male rats) at the doses of 30 mg/kg, 60 mg/kg, and 90 mg/kg on the sperm count, motility, and testicular histology. Administration were given by force-feeding between 10.00 am and 12.00 pm daily for a period of 42 days followed by sperm quality analysis and testicular histology evaluation. The sperm analysis showed that the sanrego increased the sperm count and sperm motility. The testicular histology also revealed positive effect of the plant on spermatogenesis. Overall the present study showed the sanrego is potential plant to increase male fertility.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2015-10-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/30
10.14421/biomedich.2015.42.31-33
Biology, Medicine, & Natural Product Chemistry; Vol 4, No 2 (2015); 31-33
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/30/18
Copyright (c) 2015 Muhammad Ja’far Luthfi
oai:ojs.sciencebiology.org:article/316
2023-08-09T16:42:43Z
BIOMEDICH:ART
Chemical Composition and Evaluation of Anti-tyrosinase and Anti-Oxidative Effects of Topical Cream Formulation from Acacia sieberiana, Vitellaria paradoxa and Beeswax
Tamfu, Alfred Ngenge
Koudoro, Alain Yaya
Kucukaydin, Selcuk
Olaye, Theophile
Agbangnan, Pascal Dossa Cokou
Sohounhloue, Dominique Codjo Koko
Sohounhloue, Dominique Codjo Koko
Avlessi, Felicien
Avlessi, Felicien
Biological; Natural Product Chemistry;
Acacia sieberiana; topical cream; GC-MS; phenolic composition; skin diseases; antioxidant; tyrosinase inhibition
Skin diseases can get natural therapies from medicinal plant-based products. In this study, a topical cream was formulated from ethanol extract of Acacia sieberiana, beeswax and Vitellaria paradoxa (shea) butter. GC-MS characterization with co-injection of the topical cream revealed Stearic acid (31.43%), Palmitic acid (23.15%), Oleic acid (21.44%) and Linoleic acid (16.20%) as the major components. Seven phenolic conpounds were identified and quantified by HPLC- DAD and Ferulic acid (12.810.26 mg/g) was the most abundant. The cream showed good antioxidant properties evaluated through ?-Carotene-linoleic acid assay, DPPH radical scavenging, ABTS+ assay, CUPRAC assay, and metal chelating assay. The cream had higher activity in the DPPH assay (IC50 = 32.100.84 g/mL), ABTS+ assay (IC50 = 22.490.62 g/mL) and CUPRAC assay (IC50 = 49.270.79 g/mL) than ?-Tocopherol. The antioxidant effects are an indication that the cream can reduce oxidative stress on the skin including aging, carcinogenesis and inflammation. At 100 g/mL, the topical cream showed tyrosinase inhibition of 48.230.87% regarded as relatively good compared to the standard tyrosinase inhibitor kojic acid, which showed 79.500.32% inhibition at the same concentration. The cosmetic cream was able to inhibit the melanin production rate-limiting enzyme, tyrosinase, indicating that it can control hyperpigmentation and skin spots.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2023-03-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/316
10.14421/biomedich.2023.121.251-258
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 1 (2023); 251-258
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/316/190
Copyright (c) 2023 Alfred Ngenge Tamfu, Alain Yaya Koudoro, Selcuk Kucukaydin, Theophile Olaye, Pascal Dossa Cokou Agbangnan, Dominique Codjo Koko Sohounhloue, Felicien Avlessi
oai:ojs.sciencebiology.org:article/85
2020-04-06T11:50:09Z
BIOMEDICH:ART
Novel Approaches for Detection Fluorescent-Labeled by Cellvizio Lab System on Hippocampal CA1 Region
Shaimi, Asma Ulhusna
Aasim, Wan Raihana Wan
Abdullah, Hasmah
Choon, Tan Soo
Wei, Ang Chee
Ismail, Zalina
DHEAS; Alexa Fluor 488 dye; fluorescent labeling; Cell visio Lab system
Neurosteroids have been identified in the 1981. Dehydroepiandrosterone sulphate (DHEAS) is one of the vital neurosteroids that de novo synthesized in the nervous system from cholesterol precursor (Baulieu & Robel, 1998). The aim of the study is to develop a method for fluorescence labelling. Alexa Fluor 488 dye with DHEAS antibody can binds the DHEAS antibody in the rat brain monitored by Cellvizio Lab System. DHEAS antibody (IgG isotype antibodies) was fluorescently conjugated by an amine-reactive compound, Alexa Fluor 5-SDP ester 488 dye. The resultant Alexa Fluor 488-conjugated antibodies were collected and analyzed by UV-Vis spectrophotometer instrument. The absorbance of the protein-dye conjugate at 280 nm and 494 nm were measured. Then, the degree of labeling (DOL) was calculated to achieve the desired results. Fluorescence labelling were carried out into the CA1 region of hippocampus Sprague-Dawley rat. We reported that the conjugation was successful. Optimal labeling depending on degree of labeling (DOL) needs some necessity to achieve and effective binding to the target neurosteroid, DHEAS. Cellvizio Lab system connected with Fiber Fluorescence Microscopy (FFM) probe is presented as a new approach in real-time imaging of DHEAS. In conclusion, we have developed a new method of DHEAS-Alexa Fluor fluorescence labelling to visualize and evaluate the changes of DHEAS fluorescence level in the rat hippocampus. This novel approach as a diagnostic tool and can be used to better understand the mechanisms and functions of DHEAS and other neurosteroids in future research.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2019-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/85
10.14421/biomedich.2019.81.1-6
Biology, Medicine, & Natural Product Chemistry; Vol 8, No 1 (2019); 1-6
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/85/79
Copyright (c) 2019 Asma Ulhusna Shaimi, Wan Raihana Wan Aasim, Hasmah Abdullah, Tan Soo Choon, Ang Chee Wei, Zalina Ismail
oai:ojs.sciencebiology.org:article/365
2024-03-20T02:04:38Z
BIOMEDICH:ART
In Silico BCell Epitope Design of Zika Virus Vaccine Using “Zika Virus Isolate Zika Virus/H sapiensTc/Tha/2006/CVD_06020 Complete Genome”
Savitri, Lisa
Juwita, Syntia Tanu
Antoro, Ester Lianawati
Wulansari, Ida Septika
Sukmawati, Datin An Nisa
Freitas, João Gregório
Ornai, Andre Mario Sequeira Alves
Immunology
Vaccine; Zika Virus; B-Cell Epitope; In Silico
ZIKV has been found in the cerebrospinal fluid (CSF) and brains of adults infected with viruses that manifest neurological disorders. ZIKV is a mosquito-borne single-stranded RNA virus, which belongs to the family Flaviviridae. The efforts of the scientific community have rapidly increased knowledge about this virus. However, understanding the complexities of ZIKV infection, transmission and pathogenesis remains an urgent challenge. Therefore, it is critical to study competent vectors and natural reservoirs for ZIKV, viral genetic diversity and flavivirus coinfection. Due to the great challenges to develop a ZIKV vaccine, it is still not possible to be immunized against ZIKV infection and related pathologies. The methods are nucleotide search for the Zika virus was carried out in silico, using the NCBI bioinformatics application by providing access to biomedical and genomic information. /H. sapiens-tc/THA/2006/CVD_06-020, the complete genome was then searched for FASTA, then prediction of vaccine epitope using the IEDB. The vaccine candidate peptides were analyzed for their antigenicity using VaxiJen. Proteins were classified by AllerTop to known allergenicity, then ToxinPred to predict and design toxic/non-toxic peptides. There are 30 peptide sequences are predicted to be a candidate of peptides B-cell epitope zika virus vaccine design using “zika virus isolate zika virus/H. sapiens-tc/THA/2006/CVD_06-020, complete genome”.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
LP3M Universitas Kadiri
2023-09-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/365
10.14421/biomedich.2023.122.525-531
Biology, Medicine, & Natural Product Chemistry; Vol 12, No 2 (2023); 525-531
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/365/224
Copyright (c) 2023 Lisa Savitri, Syntia Tanu Juwita, Ester Lianawati Antoro, Ida Septika Wulansari, Datin An Nisa Sukmawati, João Gregório Freitas, Andre Mario Sequeira Alves Ornai
oai:ojs.sciencebiology.org:article/146
2022-01-11T09:12:17Z
BIOMEDICH:ART
Computational Chemical Study of Pigment of Mangosteen (Garcinia mangostana) Rind Extract as Dye Compound in Dye-Sensitized Solar Cell (DSSC)
Krisdiyanto, Didik
Sudarlin, Sudarlin
Supriyati, Hikmah
α-mangostin; β-mangostin; energy HOMO-LUMO; DFT; TD-DFT; mangosteen rind; Dye-Sensitized Solar Cell
The electronic transition processes of α-mangostin and β-mangostin as dye compounds in DSSC and their ability to transfer electrons to semiconductors have been studied in theory. The research was carried out computationally using the NwChem application. The methods used are Density Functional Theory (DFT) for structure optimization and Time-Dependent DFT (TD-DFT) for electronic transitions. The results showed that the energy of HOMO β-mangostin was lower that it was more stable or easier to form bonds with semiconductors. Likewise, its LUMO energy is lower than α-mangostin that it is easier to inject electrons into the semiconductor. The energy difference of HOMO-LUMO β-mangostin is smaller than α-mangostin. This shows that β-mangostin is more easily excited or more efficient in exciting solar energy to convert it into electricity. This is consistent with the molecular spectra where λmaxβ-mangostin is at a higher wavelength. Orbital analysis shows electron injection of α-mangostin and β-mangostin into the semiconductor via double O atoms in each molecule. The injection is influenced by the bond length, where the bond length of α-mangostin to TiO2 is smaller than that of β-mangostin to TiO2.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
2021-07-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/146
10.14421/biomedich.2021.101.41-46
Biology, Medicine, & Natural Product Chemistry; Vol 10, No 1 (2021); 41-46
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/146/113
Copyright (c) 2021 Didik Krisdiyanto, Sudarlin, Hikmah Supriyati
oai:ojs.sciencebiology.org:article/243
2022-09-20T02:36:50Z
BIOMEDICH:ART
The Weight Performance Stability of Mice on Modeling Obesity-Associated Hyperglycemia Induced by Dextrose Monohydrate
Putra, Deksa Yudha Syach
Santoso, Setiyo Budi
Lutfiyati, Heni
Biology;Medicine
alloxan; preclinical research animals; streptozotocin; type 2 diabetes mellitus
Previously, streptozotocin and alloxan were employed to imitate hyperglycemia in mice. High doses of sucrose were also induced as an alternative. Due to body mass index has been associated with hyperglycemia, the evidence of weight body index in various induction alternate kinds, however, have not been fully reported. Here-in, we report on the weight performance stability of mice body weight induced by dextrose, streptozotocin, and alloxan. To begin, all mice were divided into six groups of five, with one reserve in each. Following seven days of acclimatization, the mice were induced for nine days of hyperglycemia modeling; alloxan (Groups A and D), streptozotocin (Group B and E), dextrose monohydrate (Groups C and F). On preclinical research animals modelling related to obesity-associated hyperglycemia in mice, dextrose monohydrate induction was most successful than streptozocotin and alloxan induction, which performed best during the induction period (31% weight growth) and after metformin intervention (36% weight growth). Overall, dextrose monohydrate is most suitable to be used for modeling type 2 diabetes mellitus test animals rather than alloxan and streptozocotin.
Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity
Universitas Muhammadiyah Magelang
2022-09-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://sciencebiology.org/index.php/BIOMEDICH/article/view/243
10.14421/biomedich.2022.112.169-173
Biology, Medicine, & Natural Product Chemistry; Vol 11, No 2 (2022); 169-173
2540-9328
2089-6514
eng
https://sciencebiology.org/index.php/BIOMEDICH/article/view/243/157
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/243/35
https://sciencebiology.org/index.php/BIOMEDICH/article/downloadSuppFile/243/36
Copyright (c) 2022 Deksa Yudha Syach Putra, Setiyo Budi Santoso, Heni Lutfiyati
2bcd430b84c4ec8aa7fc0f1395f249de