Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)

Rakhmiyati Rakhmiyati, Muhammad Ja’far Luthfi


Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.


Common house gecko (Hemidactylus frenatus); Autotomy; Alizarin Red- S Alcian Blue; cartilage; bone (osteon)

Full Text:



Alibardi. 2009. orphological and Cellular Aspects of Tail and Limb Regeneration in Lizards. 1 st Edition, pp: 1-49.

Alibardi. 2017. Review: Biological and Molecular Differences between Tail Regeneration and Limb Scarring in Lizard: An Inspiring Model Addressing Limb Regeneration in Amniotes. Journal of Ekperimental Zoology (Molecular and Developmental Evolution). 00B: 1–22.

Gilbert et al, 2013. The Anatomy and Histology of Caudal Autotomy and Regeneration in Lizards. Physiological and Biochemical Zoology. Vol. 86(6):631–644.

Jagnandan et al, 2014. Tail autotomy and subsequent regeneration alter the mechanics of locomotion in lizards. The Journal of Experimental Biology 217: 3891-3897.

Jacyniak et al, 2017. Tail regeneration and other phenomena of wound healing and tissue restoration in lizards. Journal of Experimental Biology. Vol. 220, pp: 2858-2869.

Kimball, 1983. Biologi. Edisi kelima. Jakarta: Erlangga.

Lin & Lin, 2017. Tail regeneration after autotomy revives survival: A case from a long-term monitored lizard population under avian predation. The Royal Society Publishing. pp: 1-9.

Londono et al, 2017. cartilage and Muscle Cell Fate and Origins during lizard Tail regeneration. Frontiers in Bioengineering and Biotechnology. Vol. 5, no. 70, pp: 1-9.

Lozito & Tuan, 2016. Lizard tail skeletal regeneration combines aspects of fracture healing and blastema-based regeneration. Development. Vol. 143, pp: 2946-2957.

Luthfi, M. J. 2002. Kalsifikasi Skeleton Aksial dan Kemampuan Autotomi Regenerat Ekor Kadal (Mabouya multifasciata Kuhl). Tesis. Universitas Gadjah Mada (Unpublished).

Maria, B. 1998. Struktur Vertebrae Caudales pada 5 Species Anggota Sub Ordo Lacertilia. Thesis. Universitas Gadjah Mada (Tidak Dipublikasikan).

Pratiwi, 2009. Struktur Organ Reproduksi dan Seksual Dimorfisme in Hemidactylus frenatus Dumeril & Bibron, 1836; Cosymbotus platyurus (Schneider, 1792); & Gekko gecko Linnaeus, 1758. Thesis Universitas Gadjah Mada (Unpublished).

Soesilo, N. P. 1992. Proses Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl). Biologi, Vol. 1, No. 4: 169-175.

Takahashi, 2009. Preferensi Pakan Cicak rumah (Hemidactylus frenatus, Gray 1825) dan Tokek (Gekko gecko, Linnaeus 1758) di Gamping, Sleman, Daerah Istimewa Yogyakarta. Thesis. Universitas Gadjah Mada (Unpublished)



  • There are currently no refbacks.

Copyright (c) 2018 Rakhmiyati, Muhammad Ja’far Luthfi

Biology, Medicine, & Natural Product Chemistry

ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Sunan Kalijaga State Islamic University &
Society for Indonesian Biodiversity

Creative Commons License
This work is licensed under a CC BY-NC