A Simple Technique for Rapid Assessment of Rat (Rattus norvegicus) Sperm Motility

Muhammad Ja’far Luthfi, Mahanem Mat Noor


Assesment of rat sperm motility can be carried out on sperm sample from epididimal source using minimal amount of equipment. This method will aid researcher and practitioner working in the field of sperm quality to determined rat sperm  motility rapidly and efficiently.


sperm quality; sperm motility; rat; haemocytometer

Full Text:



Atherton, R.W. 1977. Evaluation of sperm motility in Hafez, E.S.E.(ed). Techniques of Human Andrology. 175-179. Elsevier. Amsterdam.

Biggers, J.D., Whitten, W.K. &Whittingham, D. 1971. The culture of mouse embryos in vitro in Daniel, J.C. (ed). Methods in Mammalian Embryology. 86-116. Freeman, San Francisco, CA.

Björndahl, L. 2010. The usefulness and significance of assessing rapidly progressive

spermatozoa. Asian J Androl. 2010 12(1): 33–35.

Clegg, E.D., Perreault, S.D. & Klinefelter, G.R. 2001. Assesment of male reproductive toxicity in Hayes, A.W. (ed). Principles and methods of toxicology. 1264-1292. Fourth editions. Taylor & Francis. Philadelphia.

Das, R. P. 1985. Assessment of spermatozoal function. J. Biosci. 7 (2): 245–255.

Freund, M. & Carol, B. 1964. Factors affecting haemocytometer counts of sperm concentration in human semen. Journal of Reproduction and Fertility 8: 149-155.

Hamilton, D.W. 1975. Structure, function of the epithelium lining the ductuli efferents, ductus epididymis and ductus deferens in the rat in Hamilton, D.W. & Greep, R.O. (eds). Handbook of Physiology, Section VII, Endocrinology, vol.5, Male Reproductive System. 259-301. American Physiological Society, Washington D.C.

Kuster, C. 2005. Sperm concentration determination between hemacytometric and CASA systems: Why they can be different. Theriogenology 64: 614–617.

Luthfi, M.J. 2015. A simple and practical method for rat sperm epididymal sperm count (Rattus norvegicus). Biology, Medicine & Natural Product Chemistry 4 (1): 1-3.

Luthfi, M.J., Noor, M.M. 2015. Analisis Kualitas Sperma Tikus Percobaan (Jumlah, Motilitas, dan Morfologi). UNS Press. Surakarta. Indonesia.

Macpherson, M.L. 2001. How to Evaluate Semen in the Field. Proceedings of the Annual Convention of the AAEP. Volume 47. USA.

Martínez, C., Mar, C., Azcárate, M., Pascual, P., Aritzeta, J.M., López-Urrutia, A. 2000. Sperm motility index: a quick screening parameter from sperm quality analyser-IIB to rule out oligo- and asthenozoospermia in male fertility study. Human Reproduction 15 (8): 1727–1733.

Partodihardjo, S. 1992. Ilmu reproduksi hewan. Cetakan ke-3. 25-37. Penerbit Mutiara Sumber Widya. Jakarta.

Perreault, S.D. & Cancel, A. M. 2001. Significance of incorporating measures of sperm production and function into rat toxicology studies. Reproduction 121: 207–216.

Prasad, M.R.N., Chinoy, N.J., Kadam. K.M. 1972. Changes in succinic dehydrogenase levels in the rat epididymis under normal and altered physiologic conditions. Fertility and Sterility 23 (3): 186-190.

Strader, L.F., Linder, R. E. & Perreault. S.D. 1996. Comparison of rat epididymal sperm counts by IVOS HTM-IDENT and hemocytometer. Reproductive Toxicology 10 (6): 529-533.

Talarczyk-Desole, J., Anna Berger, A., Taszarek-Hauke1,G., Jan Hauke, J., Pawelczyk, L., Jedrzejczak, P. 2017. Manual vs. computer-assisted sperm analysis: can CASA replace manual assessment of human semen in clinical practice? Ginekologia Polska 88 (2): 56–60.

White, W.J. 2001. The use of laboratory animals in toxicologic research in Hayes A.W. (ed). Principles and Methods of Toxicology. Fourth Edition. 773-775. Taylor & Francis. Philadelphia.

WHO. 1999. Laboratory manual for the examination of human semen and semen-cervical mucus interaction . New York : Cambridge University Press.

WHO. 2010. WHO laboratory manual for theExamination and processing of human semen. Fifth Edition. WHO Press. Geneva, Switzerland.

Working, P.K. 1988. Male Reproductive Toxicology: Comparison of the Human to Animal Models. Environmental Health Perspectives 77: 37-44.

Yang, Y., Zhang, Y., Ding, J. 2019. Optimal analysis conditions for sperm motility parameters with a CASA system in a passerine bird, Passer montanus. Avian Res 10 (35) https://doi.org/10.1186/s40657-019-0174-5

DOI: https://doi.org/10.14421/biomedich.2020.92.%25p


  • There are currently no refbacks.

Copyright (c) 2020 Muhammad Ja’far Luthfi, Mahanem Mat Noor

Biology, Medicine, & Natural Product Chemistry

ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Sunan Kalijaga State Islamic University &
Society for Indonesian Biodiversity

Creative Commons License
This work is licensed under a CC BY-NC