Polyphenols Content and Antimicrobial, Antioxidant and Hemolytic Activities of Essential Oils from Four Selected Medicinal Plants Growing in Algeria

Noureddine Halla, Kebir Boucherit, Bankaddour Zeragui, Abdelkader Djelti, Ziane Belkhedim, Rachida Hassani, Saada Benatallah, Hassiba Djellouli, Oumlkheir Kacimi, Zahia Boucherit-Otmani

Abstract


The Saharan and steppe spontaneous plants are very characteristic because of their particular adaptation to the desert and extreme environment. Some species have pharmacological properties that give them a medicinal interest. The aim of the present work was to determine the polyphenol contents of essential oils obtained from four endemic plants growing in Algeria (Pituranthos scoparius, Myrtus nivellei, Rosmarinus officinalis and Mentha piperita), and study its biological activity, including antimicrobial, antioxidant, and hemolytic. The antimicrobial activity was evaluated by the microdilution method against twelve strains. The antioxidant activity was carried out by two methods (DPPH radical scavenging and reducing power). However, the hemolytic effect has been evaluated against the red blood cells. P. scoparius and M. piperita showed yields of essential oils higher than 1%. All the strains showed sensitivity against the essential oils tested with the exception of the C. albicans treated by R. officinalis essential oils. The most sensitive strain was C. albicans treated by P. scoparius essential oils by MIC of 0.0781 mg/mL, it was the same plant that shows the highest polyphenol content (14.78 ± 0.72 g GAE/g DS). The antioxidant activity by the DPPH method was greater for all essential oils tested by IC50 ranging from 0.69 ± 0.07 (R. officinalis) to 30.67 ± 2.12 mg/mL (M. nivellei). The R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid). In reducing iron, it was the R. officinalis essential oils which were found to be the most active with an EC50 concentration of 9.67 ± 1.36 mg/mL. After 120 min incubation, minimal haemolysis (10%) was obtained with essential oils of R. officinalis at a concentration of 0.39 mg/mL. We conclude that P. scoparius essential oils showed the high content of polyphenols and R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid).

Keywords


Polyphenols; Antimicrobial; Antioxidant; Essential oils; Hemolytic; Mentha piperita; Myrtus nivellei; Pituranthos scoparius; Rosmarinus officinalis; Sahara

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References


Abderrazak K, Messoued R, Azzedine Z (2013). Etude Phytochimique et de L’activité Antimicrobienne des Huiles Essentielles de Pituranthos Scoparius de la Region de Biskra (Sud-Est Algérien). Tunisian Journal of Medicinal Plants and Natural Products 10(2): xx-xx.

Akhtar N, Ihsan-ul-Haq Mirza B (2018). Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian journal of chemistry 11 (8): 1223-1235.

APS (Algeria Press Service) (2019). Environnement : l'Observatoire national de la biodiversité inscrit dans la loi de Finances 2020 (La ministre de l'Environnement et des Energies renouvelables, Fatima Zohra Zerouati). Algeria Press Service. http://www.aps.dz/economie/89734-environnement-l-observatoire-national-de-la-biodiversite-inscrit-dans-la-lf-2020.

Atanassova M, Georgieva S, Ivancheva K (2011). Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology & Metallurgy 46(1): 81-88.

Bolard J (1986). How do the polyene macrolide antibiotics affect the cellular membrane properties?. Biochimica et Biophysica Acta (BBA)-Reviews on Biomembranes 864(3-4): 258-303.

Boukhalfa D (2017). Contribution à l’étude des plantes aromatiques et médicinales de la région de l’Ahaggar (Doctoral dissertation, University of Algiers, Algeria). http://193.194.83.98/jspui/bitstream/1635/14379/1/BOUKHALFA_DJAMEL.pdf

Boutaghane N, Nacer A, Kabouche Z, Ait-Kaki B (2004). Comparative antibacterial activities of the essential oils of stems and seeds of Pituranthos scoparius from Algerian septentrional Sahara. Chemistry of natural compounds 40(6): 606-607.

Bouzabata A, Bazzali O, Cabral C, Gonçalves MJ, Cruz MT, Bighelli A, Cavaleiro C, Casanova J, Salgueiro L, Tomi F (2013). New compounds, chemical composition, antifungal activity and cytotoxicity of the essential oil from Myrtus nivellei Batt. & Trab., an endemic species of Central Sahara. Journal of ethnopharmacology 149(3): 613-620.

Celiktas OY, Kocabas EH, Bedir E, Sukan FV, Ozek T, Baser KHC (2007). Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry 100(2): 553-559.

Chikhoune A, Damjan Pavleca J, Shashkov M, Berroua Z, Chebbi K, Bougherra H, Zeroual B, Aliane K, Gagaoua M, Boudjellal A, Vovk I, Krizman M (2017). Antioxidant effect induced by the essential oil of Pituranthos scoparius in a formulation of a whey spread emulsion. Journal of Food Processing and Preservation 41(5): 1-12.

CLSI (Clinical and Laboratory Standards Institute) (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard—9th ed. CLSI document M07-A9. USA : Clinical and Laboratory Standards Institute, Wayne, PA.

CLSI (Clinical and Laboratory Standards Institute) (2008). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, Approved Standard, 3rd ed. CLSI Document M27-A3. USA : Clinical and Laboratory Standards Institute, Wayne, PA.

Derwich E, Benziane Z, Taouil R, Senhaji O, Touzani M (2010). Aromatic plants of morocco: GC/MS analysis of the essential oils of leaves of Mentha piperita. Advances in Environmental Biology 4(1): 80-86.

Dewanto V, Wu X, Adom KK, Liu RH (2002). Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of agricultural and food chemistry 50(10): 3010-3014.

Djeddi S, Bouchenah N, Settar I, Skaltsa HD (2007). Composition and antimicrobial activity of the essential oil of Rosmarinus officinalis from Algeria. Chemistry of Natural Compounds 43(4): 487-490.

El Asbahani A, Miladi K, Badri W, Sala M, Addi EA, Casabianca H, El Mousadik A, Hartmann D, Jilale A, Renaud FNR, Elaissari A (2015). Essential oils: from extraction to encapsulation. International journal of pharmaceutics 483(1-2): 220-243.

Espinel-Ingroff A, Cantón E (2007). Antifungal Susceptibility Testing of Yeasts. In: Richard S, Lynn S-M, Avery CG (eds) Antimicrobial susceptibility testing protocols. Taylor & Francis Group LLC, pp. 173-208.

Gavahian M, Farhoosh R, Farahnaky A, Javidnia K, Shahidi F (2015). Evaluation of antioxidant activities of Mentha piperita essential oils obtained by different extraction methods. Iranian Food Science and Technology Research Journal 11(6): 729-737.

Gourine N, Merrad B, Yousfi M, Stocker P, Gaydou EM (2011). Chemical composition of the essential oil of Pituranthos scoparius. Natural product communications 6(8): 1151-1154.

Haddouchi F, Chaouche TM, Halla N (2016). Phytochemical screening, antioxidant activities and hemolytic power of four Saharan plants from Algeria. Phytothérapie, 1-9.

Halla N, Boucherit K, Boucherit-Otmani Z, Touati FZ, Rahmani N, Aid I (2019a). Ammodaucus leucotrichus and Citrullus colocynthis from Algerian Sahara: Ethnopharmacological application, phytochemical screening, polyphenols content and antioxidant activity of hydromethanolic extracts. Journal of King Saud University-Science 31(4): 541-548.

Halla N, Boucherit K, Zeragui B, Hellal D, Bentoumi A, Belouafi L, Chikhi I, Boucherit-Otmani Z. (2019b). Essential Oil of Artemisia judaica L.(ssp. Sahariensis) from Algerian Sahara: Antimicrobial Effects and Mechanisms of Action. Phytothérapie. DOI 10.3166/phyto-2019-0187.

Hammiche V, Maiza K (2006). Traditional medicine in Central Sahara: pharmacopoeia of Tassili N’ajjer. Journal of ethnopharmacology 105(3): 358-367.

İşcan G, Ki̇ri̇mer N, Kürkcüoǧlu M, Başer HC, Demirci F (2002). Antimicrobial screening of Mentha piperita essential oils. Journal of agricultural and food chemistry 50(14): 3943-3946.

Jain AK, Thomas NS, Panchagnula R (2002). Transdermal drug delivery of imipramine hydrochloride.: I. Effect of terpenes. Journal of Controlled Release 79(1-3): 93-101.

Jordán MJ, Lax V, Rota MC, Lorán S, Sotomayor JA (2013). Effect of bioclimatic area on the essential oil composition and antibacterial activity of Rosmarinus officinalis L. Food Control 30(2): 463-468.

Kabouche Z, Boutaghane N, Laggoune S, Kabouche A, Ait-Kaki Z, Benlabed K (2005). Comparative antibacterial activity of five Lamiaceae essential oils from Algeria. International Journal of Aromatherapy 15(3):129-133.

Kalla A, Belkacemi D, Gherraf N, Zellagui A, Messai L, Ladjel S, Samir H, Brahim L, Chihi S (2010). Seasonal variability of essential oil content of Pituranthos scoparius. Asian Journal of Chemistry 22(4): 3065-3068.

Khaldi A (2014). La gestion non–durable de la steppe algérienne. VertigO-la revue électronique en sciences de l'environnement. https://doi.org/10.4000/vertigo.15152.

Ksouri A, Dob T, Belkebir A, Dahmane D, Nouasri A (2017). Volatile compounds and biological activities of aerial parts of Pituranthos scoparius (Coss and Dur) Schinz (Apiaceae) from Hoggar, southern Algeria. Tropical Journal of Pharmaceutical Research 16(1): 51-58.

Laghouiter OK, Gherib A, Laghouiter H (2015). Etude de l’activité antioxydante des huiles essentielles de certaines menthes cultivées dans la région de Ghardaïa. Revue ElWahat pour les Recherches et les Etudes 8(1) : 84-93.

Lee YL, Ding P (2016). Production of essential oil in plants: ontogeny, secretory structures and seasonal variations. Pertanika Journal of Scholarly Research Reviews 2(1):1-10.

Lograda T, Ramdani M, Kiram A, Chalard P, Figueredo G (2013).

Variation of essential oils composition of Pituranthos scoparius in Algeria. Global Journal of Research on Medicinal Plants & Indigenous Medicine 2(1): 1-11.

Maffei M, Canova D, Bertea CM, Scannerini S (1999). UV-A effects on photomorphogenesis and essential-oil composition in Mentha piperita. Journal of Photochemistry and Photobiology B: Biology 52(1-3): 105-110.

Mahboubi M, Kazempour N (2014). Chemical composition and antimicrobial activity of peppermint (Mentha piperita L.) Essential oil. Songklanakarin Journal of Science and Technology 36(1): 83-87.

Mendanha SA, Moura SS, Anjos JL, Valadares MC, Alonso A (2013). Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity. Toxicology in Vitro 27(1): 323-329.

Mohammadi A, Hashemi M, Hosseini M (2016). Antimicrobial Activity of Essential Oils of Cinnamomum zeylanicum, Mentha piperita, Zataria multiflora Boiss and Thymus vulgaris Against Pathogenic Bacteria. Medical Laboratory Journal 10(2): 32-40.

Halla N, Boucherit K, Boucherit-Otmani Z, Rahmoun N (2013). Erythrocyte toxicities of imidazolidinyl urea and diazolidinyl urea. Journal of Materials Science and Engineering. B 3(7B): 445.

Oyaizu M (1986). Studies on products of the browning reaction —Antioxidative Activities of Products of Browning Reaction prepared from glucosamine. The Japanese journal of nutrition and dietetics, 44(6): 307-315.

Pereira PC, Cebola MJ, Bernardo-Gil MG (2009). Evolution of the yields and composition of essential oil from Portuguese myrtle (Myrtus comunis L.) through the vegetative cycle. Molecules 14(8): 3094-3105.

Prieto P, Pineda M, Aguilar M (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry 269(2):337–41.

Qaiyumi S (2007). Macro- and Microdilution Methods of Antimicrobial Susceptibility Testing. In: Richard S, Lynn S-M, Avery CG (eds) Antimicrobial susceptibility testing protocols. Taylor & Francis Group LLC, pp. 75-80.

Rached W, Benamar H, Bennaceur M, Marouf A (2010). Screening of the antioxidant potential of some Algerian indigenous plants. Journal of Biological Sciences 10(4): 316-324.

Ramdane F, Essid R, Fares N, El Ouassis D, Aziz S, Mahammed MH, Didi Ould Hadj M, Limam F (2017). Antioxidant antileishmanial cytotoxic and antimicrobial activities of a local plant Myrtus nivellei from Algeria Sahara. Asian Pacific Journal of Tropical Biomedicine 7(8): 702-707.

Riachi LG, De Maria CA (2015). Peppermint antioxidants revisited. Food chemistry 176: 72-81.

Rohloff J, Dragland S, Mordal R, Iversen TH (2005). Effect of harvest time and drying method on biomass production, essential oil yield, and quality of peppermint (Mentha× piperita L.). Journal of agricultural and food chemistry 53(10): 4143-4148.

Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R (2005). Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food chemistry 91(4): 621-632.

Saharkhiz MJ, Motamedi M, Zomorodian K, Pakshir K, Miri R, Hemyari K (2012). Chemical composition, antifungal and antibiofilm activities of the essential oil of Mentha piperita L. ISRN pharmaceutics 2012 :1-6.

Samber N, Khan A, Varma A, Manzoor N (2015). Synergistic anti-candidal activity and mode of action of Mentha piperita essential oil and its major components. Pharmaceutical biology 53(10): 1496-1504.

Sangwan NS, Farooqi AHA, Shabih F, Sangwan RS (2001). Regulation of essential oil production in plants. Plant growth regulation 34(1): 3-21.

Seca AM, Pinto DC (2019). Biological Potential and Medical Use of Secondary Metabolites. Medicines 6(2): 66.

Sharafi SM, Rasooli I, Owlia P, Taghizadeh M, Astaneh SDA (2010). Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials. Pharmacognosy magazine 6(23): 147-153.

Silva LF, das Graças Cardoso M, Preté PSC, Teixeira ML, Nelson DL, Magalhães ML, Ferreira VRF, Souza RV, Soares LI, Marcussi S (2017). Essential Oils from Mentha viridis (L). L. and Mentha pulegium L.: Cytogenotoxic Effects on Human Cells. American Journal of Plant Sciences 8(6): 1423-1437.

Singh R, Shushni MA, Belkheir A (2015). Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry 8(3): 322-328.

Touaibia M, Chaouch FZ (2014). Evaluation of the antioxidant activity of aqueous, methanolic and ethanolic extracts of the Sahara-endemic species Myrtus nivellei Batt and Trab. (Myrtaceae). International Journal of Innovation and Applied Studies 6: 407-413.

Tyagi AK, Malik A (2011). Antimicrobial potential and chemical composition of Mentha piperita oil in liquid and vapour phase against food spoiling microorganisms. Food Control 22(11) : 1707-1714.

Vérité P, Nacer A, Kabouche Z, Seguin E (2004). Composition of seeds and stems essential oils of Pituranthos scoparius (Coss. & Dur.) Schinz. Flavour and fragrance journal 19(6): 562-564.

Wojdyło A, Oszmiański J, Czemerys R (2007). Antioxidant activity and

phenolic compounds in 32 selected herbs. Food chemistry 105(3): 940-949.

Zaouali Y, Bouzaine T, Boussaid M (2010). Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food and Chemical Toxicology 48(11): 3144-3152.

Zeragui B, Hachem K, Halla N, Kahloula K (2019). Essential Oil from Artemisia judaica L.(ssp. sahariensis) Flowers as a Natural Cosmetic Preservative: Chemical Composition, and Antioxidant and Antibacterial Activities. Journal of Essential Oil Bearing Plants 22(3): 685-694.

Zheng W, Wang SY (2001). Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food chemistry 49(11): 5165-5170.




DOI: https://doi.org/10.14421/biomedich.2020.92.65-75

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Copyright (c) 2020 Noureddine Halla, Kebir Boucherit, Bankaddour Zeragui, Abdelkader Djelti, Ziane Belkhedim, Rachida Hassani, Saada Benatallah, Hassiba Djellouli, Oumlkheir Kacimi, Zahia Boucherit-Otmani



Biology, Medicine, & Natural Product Chemistry
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