Isolation and Characterization of Sesquiterpenes from Stem Bark of Warburgia ugandensis Sprague

Teshome Gonfa Hordofa


Warburgia ugandensis Sprague is one of the medicinal plants traditionally used to treat a number of diseases like asthma, cough, diarrhea, common cold, stomachache and toothache in Ethiopia. However, there is still insufficient information on the isolation and evaluation of bioactive compounds from this plant species. Extraction, purification and isolation of the stem bark of this plant by dichloromethane and chloroform as solvents afforded two sesquiterpenes; namely, WU-1 (ugandensidial) and WU-2 (cinnamolide-3β-acetate) respectively. The structural elucidations of these bioactive compounds were accomplished by using a variety of spectroscopic methods (IR, UV and NMR). The spectroscopic results compared with the reported data in the literature.


Medicinal plants; Natural products; Sesquiterpenes; Warburgia ugandensis Sprague

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Butler, M.S. (2004). The Role of Natural Product Chemistry in Drug Discovery. J Nat Prod., 67(12) 2141-2153. DOI: 10.1021/np040106y

Dagne, E. (2009). Natural Database for Africa (NDA) On CD-ROM. Version1. Addis Ababa, Ethiopia.

Dharani, N., Rukunga, G., Yenesew A., Mbora A., Mwaura L., Dawson I.; Jamnadass R. (2008). Common Antimalarial Trees and Shrubs of East Africa.1st edition. World Agro-forestry Centre: Nairobi, Kenya. ISBN: 978-92-9059-238-9. DOI: 10.1016/j.phytochem.2005.07.018

Drage, S., Mitter, B., Muchugi, A., Trols, A., Jamnadass, R. H., Sessitsch, A., Hadacek, F. (2014). Antimicrobial drimane sesquiterpenes and their effect on endophyte communities in the medical tree Warburgia ugandensis. Front Microbiol. 5(13). DOI: 10.3389/fmicb.2014.00013

Geyid, A., Abebe, D., Debella, A., Makonnen, Z., Aberre, F., Teka, F., Kebede, T., Urga, K., Yersaw, K., Biza, T., Mariam, B.H., Guta. M. (2005). Screening of some medicinal plants of Ethiopia for their anti-macrobial properties and chemical profiles. J. Ethnopharmacol, 97, 421-427. DOI: 10.1016/j.jep.2004.08.021

Gómez, G. S., Pelacho, A., Gené, A., Capell, T., Christou, P. (2007). The genetic Manipulation of Medicinal and Aromatic Plants. Plant Cell Rep.,26, 1689-1715.DOI: 10.1007/s00299-007-0384-x

Karani, L. W., Tolo, F. M., Karanja, S.M., Khayeka, C. W. (2013). Safety and efficacy of Prunus africana and Warburgia ugandensis against induced asthma in BALB/c Mice. Eur. J. Med. Plants. 3(3) 345-368. DOI:10.9734/EJMP/2013/3669

Kioy, D., Gray, A. I., Waterman, P. G. (1990). A comparative study of the stem barks drimane sesquiterpenes and leaf volatile oils of W. ugandensis and W. stuhlmannii. Phytochemistry, 29(11) 3535–3538.DIO: 10.1016/0031-9422(90)85270-P

Maroyi, A. (2013). Warburgia salutaris (Bertol. f.) Chiov; A multi-use ethnomedicinal plant species. J. Med. Plant Res., 7(2) 53-60. DOI: 10.5897/JMPR12.1019

Maundu, P., Tengnas, B. (2005). Useful Trees and Shrubs for Kenya. World Agroforestry Centre Eastern and Central Africa Regional Programme (ICRAF-ECA), Nairobi, Kenya. ISBN 9966-70-8.

Mbwambo, Z. H., Erasto, P., Innocent, E., Masimba P. J. (2009). Antimicrobial and cytotoxic activities of fresh leaf extracts of Warburgia ugandensis. Tanzan. J. Health Res,11 (2) 75-78.

Mohammed G. R., Extraction, Isolation and Characterization of Natural Products from Medicinal Plants. International Journal of Basic Sciences and Applied Computing (IJBSAC)ISSN: 2394-367X, 2 (6) 2018

Muchugi, A., Muluvi, G. M., Kindt, R., Kadu, C. A. C., Simons, A. J., Jamnadass, R.H. (2008). Genetic structuring of important medicinal species of genus Warburgiaas revealed by AFLP analysis. Tree Genet Genomes, 4(4) 787-795. DOI: 10.1007/s11295-008-0151-3

Ngure, P. K., Tonui, W. K., Ingonga. J., Mutai, Ch., Kigondu, E., Ng’ang’a, Z., Rukunga, G., Kimutai, A. (2009). In vitro antileishmanial activity of extracts of Warburgia ugandensis (Canellaceae), a Kenyan medicinal plant. J. Med. Plants Res., 3(2)61-66. DOI 10.3923/rjmp.2009.61.68

Rabe, T., Van Staden, J. (2000). Isolation of an antibacterial sesquiterpenoid from Warburgia salutaris. J. Ethnopharmacol., 73(1-2) 171–174. DOI:10.1016/s0378-8741(00)00293-2

Rajab, M.S., Ndegwa, J.M. (2000). 11α-Hydroxymuzigadiolide, a novel drimane sesquiterpene from the stem barks of Warburgia ugandensis. Bull. Chem. Soc. Ethiop. 14(1) 45–49. ISSN 1011-3294

Rukutt, J. K., Ngigi, A. N., Ndalut, P. K. (2005). Evaluation of natural products as possible alternatives to methyl bromide in soil fumigation. J. Agri. Sci. Tech, 7(1) 41-52. DOI: 10.1016/j.phymed.2003.08.005

Saiprasanna, B., Manohar, B.S., Roja, R. Y., Prasanta, K. C., Rajeshree, P. (2012). Phytochemical investigation and study on antioxidant properties of Ocimumcanum hydro-alcoholic leaf extracts. J. Drug Deliv. Ther., 2(4) 122 -128. DOI:10.22270/jddt.v2i4.198

Sasidharan S., Chen Y., Saravanan D., Sundram K. M., Yoga L. L. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med., 8(1) 1-10, DOI: 10.4314/ajtcam.v8i1.60483.

Sukirtha, K., Growther, L. (2012). Antibacterial, antifungal and phytochemical analysis of selected medicinal plants.J. Nat. Prod. Plant Resour., 2(6) 644-648. (

Wube, A. A., Bucar, F., Gibbons, S., Asres, K., Rattray, L., Croft, S.L. (2010). Antiprotozoal activity of drimane and coloratane sesquiterpenes towards Trypanosoma brucei rhodesiense and Plasmodium falciparum in vitro. Phytother Res., 24(10) 1468-1472. DOI: 10.1002/ptr.3126

Wube, A. A., Bucar, F., Gibbons, S., Asres, K. (2005). Sesquiterpenes from Warburgia ugandensis and their antimycobacterial activity. Phytochemistry, 66(19) 2309-2315.

Xu, M., Litaudon, M., Krief, S., Martin, M., Kasenene, J., Kiremire, B., Dumontet, V., Guéritte, F. (2009). Ugandenial A, a new drimane-type sesquiterpenoid from Warburgia ugandensis. Molecules, 14(10) 3844-3850. DOI: 10.3390/molecules14103844



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Copyright (c) 2020 Teshome Gonfa Hordofa

Biology, Medicine, & Natural Product Chemistry

ISSN 2089-6514 (paper) - ISSN 2540-9328 (online)
Sunan Kalijaga State Islamic University &
Society for Indonesian Biodiversity

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